Q9Y496 (KIF3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIF3A

Alternative name(s):
Microtubule plus end-directed kinesin motor 3A

Gene names

Name:	KIF3A
Synonyms:	KIF3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation By similarity.
Subunit structure	Heterodimer of KIF3A and KIF3B. Interacts with PIFO. Interacts with CLN3. Ref.6 Ref.9
Subcellular location	Cytoplasm › cytoskeleton Probable. Cell projection › cilium By similarity.
Sequence similarities	Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain.
Sequence caution	The sequence AAC04475.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD93017.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cilium biogenesis/degradation
Cellular component	Cell projection Cilium Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior pattern specification Inferred from electronic annotation. Source: Compara antigen processing and presentation of exogenous peptide antigen via MHC class II Traceable author statement. Source: Reactome blood coagulation Traceable author statement. Source: Reactome cilium assembly Inferred from sequence or structural similarity. Source: UniProtKB determination of left/right symmetry Inferred from electronic annotation. Source: Compara dorsal/ventral neural tube patterning Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from electronic annotation. Source: Compara kidney development Inferred from electronic annotation. Source: Compara organelle organization Traceable author statement PubMed 1447303. Source: ProtInc plus-end-directed vesicle transport along microtubule Traceable author statement PubMed 16298999. Source: BHF-UCL smoothened signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	centrosome Inferred from direct assay PubMed 16298999. Source: BHF-UCL cytosol Traceable author statement. Source: Reactome kinesin II complex Inferred from direct assay PubMed 16298999. Source: BHF-UCL photoreceptor connecting cilium Inferred from electronic annotation. Source: Compara primary cilium Inferred from sequence or structural similarity. Source: UniProtKB spindle microtubule Inferred from direct assay PubMed 16298999. Source: BHF-UCL
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW plus-end-directed microtubule motor activity Traceable author statement PubMed 16298999. Source: BHF-UCL
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DISC1	Q9NRI5	3	EBI-1104844,EBI-529989

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 699	699	Kinesin-like protein KIF3A		PRO_0000125393
Regions
	Domain	1 – 350	350	Kinesin-motor
	Nucleotide binding	100 – 107	8	ATP By similarity
	Region	697 – 699	3	Globular
	Coiled coil	355 – 590	236	Potential
Amino acid modifications
	Modified residue	633	1	Phosphothreonine By similarity
	Modified residue	687	1	Phosphoserine Ref.5 Ref.7
Natural variations
	Natural variant	172	1	K → I. Ref.4 Corresponds to variant rs17854353 [ dbSNP | Ensembl ].		VAR_055319
Experimental info
	Sequence conflict	151	1	E → G in AAH45542. Ref.4
	Sequence conflict	170	1	E → A in AAC72294. Ref.1
	Sequence conflict	409	1	R → RDQA in BAD93017. Ref.2
	Sequence conflict	409	1	R → RDQT in AAH45542. Ref.4
	Sequence conflict	409	1	R → RIQI in AAC72294. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Y496 [UniParc]. Last modified May 18, 2010. Version 4. Checksum: A0BF5BB1F699DF12	FASTA	699	80,041
	10 20 30 40 50 60 MPINKSEKPE SCDNVKVVVR CRPLNEREKS MCYKQAVSVD EMRGTITVHK TDSSNEPPKT 70 80 90 100 110 120 FTFDTVFGPE SKQLDVYNLT ARPIIDSVLE GYNGTIFAYG QTGTGKTFTM EGVRAIPELR 130 140 150 160 170 180 GIIPNSFAHI FGHIAKAEGD TRFLVRVSYL EIYNEEVRDL LGKDQTQRLE VKERPDVGVY 190 200 210 220 230 240 IKDLSAYVVN NADDMDRIMT LGHKNRSVGA TNMNEHSSRS HAIFTITIEC SEKGIDGNMH 250 260 270 280 290 300 VRMGKLHLVD LAGSERQAKT GATGQRLKEA TKINLSLSTL GNVISALVDG KSTHVPYRNS 310 320 330 340 350 360 KLTRLLQDSL GGNSKTMMCA NIGPADYNYD ETISTLRYAN RAKNIKNKAR INEDPKDALL 370 380 390 400 410 420 RQFQKEIEEL KKKLEEGEEI SGSDISGSEE DDDEEGEVGE DGEKRKKRRG KKKVSPDKMI 430 440 450 460 470 480 EMQAKIDEER KALETKLDME EEERNKARAE LEKREKDLLK AQQEHQSLLE KLSALEKKVI 490 500 510 520 530 540 VGGVDLLAKA EEQEKLLEES NMELEERRKR AEQLRRELEE KEQERLDIEE KYTSLQEEAQ 550 560 570 580 590 600 GKTKKLKKVW TMLMAAKSEM ADLQQEHQRE IEGLLENIRQ LSRELRLQML IIDNFIPRDY 610 620 630 640 650 660 QEMIENYVHW NEDIGEWQLK CVAYTGNNMR KQTPVPDKKE KDPFEVDLSH VYLAYTEESL 670 680 690 RQSLMKLERP RTSKGKARPK TGRRKRSAKP ETVIDSLLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Photoreceptor localization of the KIF3A and KIF3B subunits of the heterotrimeric microtubule motor kinesin II in vertebrate retina." Whitehead J.L., Wang S.Y., Bost-Usinger L., Hoang E., Frazer K.A., Burnside B. Exp. Eye Res. 69:491-503(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-172. Tissue: Testis.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-687, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Pitchfork regulates primary cilia disassembly and left-right asymmetry." Kinzel D., Boldt K., Davis E.E., Burtscher I., Trumbach D., Diplas B., Attie-Bitach T., Wurst W., Katsanis N., Ueffing M., Lickert H. Dev. Cell 19:66-77(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PIFO.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-687, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Neuronal ceroid lipofuscinosis protein CLN3 interacts with motor proteins and modifies location of late endosomal compartments." Uusi-Rauva K., Kyttala A., van der Kant R., Vesa J., Tanhuanpaa K., Neefjes J., Olkkonen V.M., Jalanko A. Cell. Mol. Life Sci. 69:2075-2089(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CLN3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF041853 mRNA. Translation: AAC72294.1. AB209780 mRNA. Translation: BAD93017.1. Different initiation. AC004039 Genomic DNA. No translation available. AC004237 Genomic DNA. Translation: AAC04475.1. Sequence problems. BC045542 mRNA. Translation: AAH45542.1.
IPI	IPI00000455.
RefSeq	NP_008985.3. NM_007054.5.
UniGene	Hs.43670.
3D structure databases
ProteinModelPortal	Q9Y496.
SMR	Q9Y496. Positions 13-391.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-33237N.
IntAct	Q9Y496. 7 interactions.
STRING	9606.ENSP00000368020.
PTM databases
PhosphoSite	Q9Y496.
Polymorphism databases
DMDM	296439481.
Proteomic databases
PaxDb	Q9Y496.
PRIDE	Q9Y496.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378746; ENSP00000368020; ENSG00000131437.
GeneID	11127.
KEGG	hsa:11127.
UCSC	uc003kxo.3. human.
Organism-specific databases
CTD	11127.
GeneCards	GC05M132059.
H-InvDB	HIX0024788.
HGNC	HGNC:6319. KIF3A.
HPA	CAB037085.
MIM	604683. gene.
neXtProt	NX_Q9Y496.
PharmGKB	PA30102.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5059.
HOGENOM	HOG000116164.
HOVERGEN	HBG052255.
KO	K10394.
Enzyme and pathway databases
Pathway_Interaction_DB	hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins.
Reactome	REACT_11123. Membrane Trafficking. REACT_604. Hemostasis. REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q9Y496.
Bgee	Q9Y496.
CleanEx	HS_KIF3A.
Genevestigator	Q9Y496.
GermOnline	ENSG00000131437. Homo sapiens.
Family and domain databases
Gene3D	3.40.850.10. 1 hit.
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5544.
ChiTaRS	KIF3A. human.
GenomeRNAi	11127.
NextBio	42288.
SOURCE	Search...

Entry information

Entry name

KIF3A_HUMAN

Accession

Primary (citable) accession number: Q9Y496
Secondary accession number(s): A8MSW9 , Q59EN1, Q86XE9, Q9Y6V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 104 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.