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Protein

Plakophilin-3

Gene

PKP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in junctional plaques.

GO - Molecular functioni

  • alpha-catenin binding Source: BHF-UCL
  • cell adhesion molecule binding Source: UniProtKB

GO - Biological processi

  • desmosome assembly Source: UniProtKB
  • establishment of protein localization to plasma membrane Source: UniProtKB
  • single organismal cell-cell adhesion Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

SignaLinkiQ9Y446.

Names & Taxonomyi

Protein namesi
Recommended name:
Plakophilin-3
Gene namesi
Name:PKP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9025. PKP3.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: ProtInc
  • desmosome Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33358.

Polymorphism and mutation databases

BioMutaiPKP3.
DMDMi20139301.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 797797Plakophilin-3PRO_0000064287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei123 – 1231PhosphoserineBy similarity
Modified residuei180 – 1801PhosphoserineCombined sources
Modified residuei183 – 1831PhosphoserineCombined sources
Modified residuei238 – 2381PhosphoserineCombined sources
Modified residuei240 – 2401PhosphoserineCombined sources
Modified residuei250 – 2501PhosphothreonineCombined sources
Modified residuei313 – 3131PhosphoserineCombined sources
Modified residuei314 – 3141PhosphoserineCombined sources
Modified residuei331 – 3311PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y446.
MaxQBiQ9Y446.
PaxDbiQ9Y446.
PeptideAtlasiQ9Y446.
PRIDEiQ9Y446.

PTM databases

iPTMnetiQ9Y446.
PhosphoSiteiQ9Y446.
SwissPalmiQ9Y446.

Expressioni

Tissue specificityi

Isoform PKP3a is found in desmosomes of most simple and stratified epithelia. Not found in foreskin fibroblasts and various sarcoma-derived cell lines. Beside dendritic reticular cells of lymphatic follicles not found in non-epithelial desmosome-bearing tissues. Isoform PKP3b is abundant in the desmosomes of stratified epithelial cell but absent in simple epithelial cells, it is also expressed in the colon and its tumors.1 Publication

Gene expression databases

BgeeiQ9Y446.
CleanExiHS_PKP3.
ExpressionAtlasiQ9Y446. baseline and differential.
GenevisibleiQ9Y446. HS.

Organism-specific databases

HPAiCAB012993.

Interactioni

GO - Molecular functioni

  • alpha-catenin binding Source: BHF-UCL
  • cell adhesion molecule binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116357. 54 interactions.
IntActiQ9Y446. 12 interactions.
MINTiMINT-3975143.
STRINGi9606.ENSP00000331678.

Structurei

3D structure databases

ProteinModelPortaliQ9Y446.
SMRiQ9Y446. Positions 318-781.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati305 – 34844ARM 1Add
BLAST
Repeati351 – 39040ARM 2Add
BLAST
Repeati393 – 43240ARM 3Add
BLAST
Repeati449 – 48739ARM 4Add
BLAST
Repeati491 – 53646ARM 5Add
BLAST
Repeati596 – 63742ARM 6Add
BLAST
Repeati645 – 68440ARM 7Add
BLAST
Repeati689 – 73042ARM 8Add
BLAST

Sequence similaritiesi

Belongs to the beta-catenin family.Curated
Contains 8 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1048. Eukaryota.
ENOG410Y21Q. LUCA.
GeneTreeiENSGT00760000119167.
HOGENOMiHOG000115567.
HOVERGENiHBG106682.
InParanoidiQ9Y446.
OMAiNARNKDE.
OrthoDBiEOG7RFTGQ.
PhylomeDBiQ9Y446.
TreeFamiTF321877.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR028434. Plakophilin-3.
IPR028435. Plakophilin/d_Catenin.
[Graphical view]
PANTHERiPTHR10372. PTHR10372. 1 hit.
PTHR10372:SF1. PTHR10372:SF1. 1 hit.
PfamiPF00514. Arm. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 4 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform PKP3a (identifier: Q9Y446-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQDGNFLLSA LQPEAGVCSL ALPSDLQLDR RGAEGPEAER LRAARVQEQV
60 70 80 90 100
RARLLQLGQQ PRHNGAAEPE PEAETARGTS RGQYHTLQAG FSSRSQGLSG
110 120 130 140 150
DKTSGFRPIA KPAYSPASWS SRSAVDLSCS RRLSSAHNGG SAFGAAGYGG
160 170 180 190 200
AQPTPPMPTR PVSFHERGGV GSRADYDTLS LRSLRLGPGG LDDRYSLVSE
210 220 230 240 250
QLEPAATSTY RAFAYERQAS SSSSRAGGLD WPEATEVSPS RTIRAPAVRT
260 270 280 290 300
LQRFQSSHRS RGVGGAVPGA VLEPVARAPS VRSLSLSLAD SGHLPDVHGF
310 320 330 340 350
NSYGSHRTLQ RLSSGFDDID LPSAVKYLMA SDPNLQVLGA AYIQHKCYSD
360 370 380 390 400
AAAKKQARSL QAVPRLVKLF NHANQEVQRH ATGAMRNLIY DNADNKLALV
410 420 430 440 450
EENGIFELLR TLREQDDELR KNVTGILWNL SSSDHLKDRL ARDTLEQLTD
460 470 480 490 500
LVLSPLSGAG GPPLIQQNAS EAEIFYNATG FLRNLSSASQ ATRQKMRECH
510 520 530 540 550
GLVDALVTSI NHALDAGKCE DKSVENAVCV LRNLSYRLYD EMPPSALQRL
560 570 580 590 600
EGRGRRDLAG APPGEVVGCF TPQSRRLREL PLAADALTFA EVSKDPKGLE
610 620 630 640 650
WLWSPQIVGL YNRLLQRCEL NRHTTEAAAG ALQNITAGDR RWAGVLSRLA
660 670 680 690 700
LEQERILNPL LDRVRTADHH QLRSLTGLIR NLSRNARNKD EMSTKVVSHL
710 720 730 740 750
IEKLPGSVGE KSPPAEVLVN IIAVLNNLVV ASPIAARDLL YFDGLRKLIF
760 770 780 790
IKKKRDSPDS EKSSRAASSL LANLWQYNKL HRDFRAKGYR KEDFLGP
Length:797
Mass (Da):87,082
Last modified:November 1, 1999 - v1
Checksum:iD43C7E77FA805E7E
GO
Isoform PKP3b (identifier: Q9Y446-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MQDGNFLLSALQ → MESWTPRPSAVASGMSWEAGGIRTTSR

Show »
Length:812
Mass (Da):88,655
Checksum:i174072FD73D1512A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti719 – 7191V → A in BAD97231 (Ref. 4) Curated
Sequence conflicti730 – 7301V → G in BAD97231 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1212MQDGN…LSALQ → MESWTPRPSAVASGMSWEAG GIRTTSR in isoform PKP3b. 2 PublicationsVSP_053646Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98265 mRNA. Translation: CAB44310.1.
AF053719 mRNA. Translation: AAF23050.1.
FN421477 mRNA. Translation: CAZ65731.1.
AK223511 mRNA. Translation: BAD97231.1.
BC000081 mRNA. Translation: AAH00081.1.
DA439471 mRNA. No translation available.
CCDSiCCDS7695.1. [Q9Y446-1]
RefSeqiNP_001289958.1. NM_001303029.1. [Q9Y446-2]
NP_009114.1. NM_007183.3. [Q9Y446-1]
UniGeneiHs.534395.

Genome annotation databases

EnsembliENST00000331563; ENSP00000331678; ENSG00000184363. [Q9Y446-1]
GeneIDi11187.
KEGGihsa:11187.
UCSCiuc001lpc.3. human. [Q9Y446-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98265 mRNA. Translation: CAB44310.1.
AF053719 mRNA. Translation: AAF23050.1.
FN421477 mRNA. Translation: CAZ65731.1.
AK223511 mRNA. Translation: BAD97231.1.
BC000081 mRNA. Translation: AAH00081.1.
DA439471 mRNA. No translation available.
CCDSiCCDS7695.1. [Q9Y446-1]
RefSeqiNP_001289958.1. NM_001303029.1. [Q9Y446-2]
NP_009114.1. NM_007183.3. [Q9Y446-1]
UniGeneiHs.534395.

3D structure databases

ProteinModelPortaliQ9Y446.
SMRiQ9Y446. Positions 318-781.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116357. 54 interactions.
IntActiQ9Y446. 12 interactions.
MINTiMINT-3975143.
STRINGi9606.ENSP00000331678.

PTM databases

iPTMnetiQ9Y446.
PhosphoSiteiQ9Y446.
SwissPalmiQ9Y446.

Polymorphism and mutation databases

BioMutaiPKP3.
DMDMi20139301.

Proteomic databases

EPDiQ9Y446.
MaxQBiQ9Y446.
PaxDbiQ9Y446.
PeptideAtlasiQ9Y446.
PRIDEiQ9Y446.

Protocols and materials databases

DNASUi11187.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331563; ENSP00000331678; ENSG00000184363. [Q9Y446-1]
GeneIDi11187.
KEGGihsa:11187.
UCSCiuc001lpc.3. human. [Q9Y446-1]

Organism-specific databases

CTDi11187.
GeneCardsiPKP3.
HGNCiHGNC:9025. PKP3.
HPAiCAB012993.
MIMi605561. gene.
neXtProtiNX_Q9Y446.
PharmGKBiPA33358.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1048. Eukaryota.
ENOG410Y21Q. LUCA.
GeneTreeiENSGT00760000119167.
HOGENOMiHOG000115567.
HOVERGENiHBG106682.
InParanoidiQ9Y446.
OMAiNARNKDE.
OrthoDBiEOG7RFTGQ.
PhylomeDBiQ9Y446.
TreeFamiTF321877.

Enzyme and pathway databases

SignaLinkiQ9Y446.

Miscellaneous databases

ChiTaRSiPKP3. human.
GeneWikiiPKP3.
GenomeRNAii11187.
PROiQ9Y446.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y446.
CleanExiHS_PKP3.
ExpressionAtlasiQ9Y446. baseline and differential.
GenevisibleiQ9Y446. HS.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR028434. Plakophilin-3.
IPR028435. Plakophilin/d_Catenin.
[Graphical view]
PANTHERiPTHR10372. PTHR10372. 1 hit.
PTHR10372:SF1. PTHR10372:SF1. 1 hit.
PfamiPF00514. Arm. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 4 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Plakophilin 3 -- a novel cell-type-specific desmosomal plaque protein."
    Schmidt A., Langbein L., Praetzel S., Rode M., Rackwitz H.-R., Franke W.W.
    Differentiation 64:291-306(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PKP3A).
    Tissue: Colon carcinoma.
  2. "Plakophilin-3, a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells."
    Bonne S., van Hengel J., Nollet F., Kools P., van Roy F.
    J. Cell Sci. 112:2265-2276(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PKP3A).
  3. "An alternative promoter of the human plakophilin-3 gene controls the expression of the new isoform PKP3b."
    Muhmer M., Ditthardt D., Jakel J., Wischmann V., Moll R., Schmidt A.
    Cell Tissue Res. 355:143-162(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PKP3B), ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY.
  4. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PKP3A).
    Tissue: Colon.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PKP3A).
    Tissue: Placenta.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-178 (ISOFORM PKP3B).
    Tissue: Tongue.
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180; SER-238; SER-240; SER-313 AND SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238; SER-313 AND SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180; SER-183; SER-238; THR-250; SER-313; SER-314 AND SER-331, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.

Entry informationi

Entry nameiPKP3_HUMAN
AccessioniPrimary (citable) accession number: Q9Y446
Secondary accession number(s): F8J390, Q53EX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: November 1, 1999
Last modified: July 6, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.