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Reviewed, UniProtKB/Swiss-Prot Q9Y3V2 (RWDD3_HUMAN)

Last modified January 19, 2010. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    RWD domain-containing protein 3
Alternative name(s):
    RWD-containing sumoylation enhancer
Gene names
Name: RWDD3
Synonyms: RSUME
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination. Ref.6

Subcellular location

Nucleus. Cytoplasm. Note: Colocalizes with UBC9/UBE2I in nuclear spots. Ref.6

Tissue specificity

Expressed in a wide number of tissues with highest expression in cerebellum, pituitary, heart, kidney, liver, stomach, pancreas, prostate and spleen. Low levels in thalamus, spinal cord, esophagus, thymus, lung and peripheral blood leukocytes. Ref.6

Induction

Induced by hypoxia. Ref.6

Domain

The RWD domain is required for the sumoylation enhancement activity By similarity.

Sequence similarities

Contains 1 RWD domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionprotein binding Ref.6

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y3V2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y3V2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     192-195: EYLI → VPKS
     196-267: Missing.
Isoform 3 (identifier: Q9Y3V2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MAEPVQEELSVLAAIFCRPHEWEVLSRS → MFLSCGSLHQRGP
     177-267: KIILILLQGD...FSEFVLALVK → VLDSSENLQSRCGLKWKEMQREND

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267RWD domain-containing protein 3
PRO_0000097545

Regions

Domain7 – 114108RWD

Natural variations

Alternative sequence1 – 2828MAEPV…VLSRS → MFLSCGSLHQRGP in isoform 3.
VSP_035411
Alternative sequence177 – 26791KIILI…LALVK → VLDSSENLQSRCGLKWKEMQ REND in isoform 3.
VSP_035412
Alternative sequence192 – 1954EYLI → VPKS in isoform 2.
VSP_034176
Alternative sequence196 – 26772Missing in isoform 2.
VSP_034177
Natural variant471A → V: dbSNP rs259358. Ref.4
VAR_034420
Natural variant861K → N: dbSNP rs2296308. Ref.4
VAR_024346

Experimental info

Mutagenesis61 – 622YP → AA: Abolishes enhancement of IKBA sumoylation. Ref.6
Sequence conflict791Q → R in CAG38524. Ref.3

Secondary structure

........................ 267
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 10, 2008. Version 3.
Checksum: 5C7943F4D4CF41EA

FASTA26730,530
        10         20         30         40         50         60 
MAEPVQEELS VLAAIFCRPH EWEVLSRSET DGTVFRIHTK AEGFMDADIP LELVFHLPVN 

        70         80         90        100        110        120 
YPSCLPGISI NSEQLTRAQC VTVKEKLLEQ AESLLSEPMV HELVLWIQQN LRHILSQPET 

       130        140        150        160        170        180 
GSGSEKCTFS TSTTMDDGLW ITLLHLDHMR AKTKYVKIVE KWASDLRLTG RLMFMGKIIL 

       190        200        210        220        230        240 
ILLQGDRNNL KEYLILQKTS KVDVDSSGKK CKEKMISVLF ETKVQTEHKR FLAFEVKEYS 

       250        260 
ALDELQKEFE TAGLKKLFSE FVLALVK 

« Hide

Isoform 2.

Checksum: BED3EE497BEC142B
Show »

FASTA19522,140
Isoform 3.

Checksum: D5AE4A4E63917E8B
Show »

FASTA18521,048

References

« Hide 'large scale' references
[1]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Kidney.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Thymus.
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS VAL-47 AND ASN-86.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[6]"RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia."
Carbia-Nagashima A., Gerez J., Perez-Castro C., Paez-Pereda M., Silberstein S., Stalla G.K., Holsboer F., Arzt E.
Cell 131:309-323(2007) [PubMed: 17956732] [Abstract]
Cited for: FUNCTION, INTERACTION WITH UBE2I, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF 61-TYR-PRO-62.
[7]"Solution structure of the RWD domain of human RWD domain containing protein 3."
RIKEN structural genomics initiative (RSGI)
Submitted (AUG-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 1-121.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL050062 mRNA. Translation: CAB43254.2.
AK292665 mRNA. Translation: BAF85354.1.
AK292699 mRNA. Translation: BAF85388.1.
CR533493 mRNA. Translation: CAG38524.1.
AC092802 Genomic DNA. No translation available.
BC010936 mRNA. No translation available.
BC125142 mRNA. Translation: AAI25143.1.
IPIIPI00642965.
IPI00646504.
IPI00790337.
RefSeqNP_001121614.1.
UniGeneHs.709591

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EBKNMR-A1-121[»]
ModBaseSearch...

Protein-protein interaction databases

IntActQ9Y3V2. 4 interactions.
STRINGQ9Y3V2.

Genome annotation databases

EnsemblENST00000370202; ENSP00000359221; ENSG00000122481; Homo sapiens. [Genome view]
GeneID25950.
KEGGhsa:25950.
UCSCuc001drf.2. human.
uc009wdu.1. human.

Organism-specific databases

CTD25950.
GeneCardsGC01P095411.
H-InvDBHIX0000800.
HGNCHGNC:21393. RWDD3.
PharmGKBPA134974302.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG14218.
HOGENOMHBG445870.
HOVERGENQ9Y3V2.
InParanoidQ9Y3V2.

Gene expression databases

ArrayExpressQ9Y3V2.
BgeeQ9Y3V2.
CleanExHS_RWDD3.
GenevestigatorQ9Y3V2.
GermOnlineENSG00000122481. Homo sapiens.

Family and domain databases

InterProIPR006575. RWD.
IPR016135. UBQ-conjugat/RWD-like.
[Graphical view]
Gene3DG3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PfamPF05773. RWD. 1 hit.
[Graphical view]
SMARTSM00591. RWD. 1 hit.
[Graphical view]
PROSITEPS50908. RWD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRWDD3_HUMAN
AccessionPrimary (citable) accession number: Q9Y3V2
Secondary accession number(s): A6NP44 expand/collapse secondary AC list , A8K9F0, Q08AJ7, Q6FID3, Q9BX35
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 10, 2008
Last modified: January 19, 2010
This is version 70 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents