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Q9Y3S1

- WNK2_HUMAN

UniProt

Q9Y3S1 - WNK2_HUMAN

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Protein

Serine/threonine-protein kinase WNK2

Gene

WNK2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activation requires autophosphorylation of Ser-356. Phosphorylation of Ser-352 also promotes increased activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei207 – 2071ATPBy similarityPROSITE-ProRule annotation
Active sitei323 – 3231Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi201 – 2099ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. intracellular signal transduction Source: UniProtKB
  2. negative regulation of cell proliferation Source: UniProtKB
  3. negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
  4. positive regulation of sodium ion transmembrane transporter activity Source: UniProtKB
  5. protein autophosphorylation Source: UniProtKB
  6. protein phosphorylation Source: UniProtKB
  7. regulation of ion homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_160189. Stimuli-sensing channels.
SignaLinkiQ9Y3S1.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase WNK2 (EC:2.7.11.1)
Alternative name(s):
Antigen NY-CO-43
Protein kinase lysine-deficient 2
Protein kinase with no lysine 2
Serologically defined colon cancer antigen 43
Gene namesi
Name:WNK2
Synonyms:KIAA1760, PRKWNK2, SDCCAG43
ORF Names:P/OKcl.13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:14542. WNK2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33783.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22972297Serine/threonine-protein kinase WNK2PRO_0000086822Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451Phosphoserine1 Publication
Modified residuei352 – 3521Phosphoserine; by autocatalysisBy similarity
Modified residuei356 – 3561Phosphoserine; by autocatalysisBy similarity
Modified residuei560 – 5601Phosphoserine1 Publication
Modified residuei1150 – 11501Phosphoserine1 Publication
Modified residuei1262 – 12621Phosphoserine1 Publication
Modified residuei1588 – 15881Phosphoserine1 Publication
Modified residuei1685 – 16851Phosphoserine1 Publication
Modified residuei1736 – 17361Phosphoserine1 Publication
Modified residuei1817 – 18171Phosphoserine3 Publications
Modified residuei1818 – 18181Phosphoserine3 Publications
Modified residuei1862 – 18621Phosphoserine2 Publications
Modified residuei1889 – 18891PhosphoserineBy similarity
Modified residuei2067 – 20671Phosphoserine1 Publication

Post-translational modificationi

Autophosphorylated.5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Y3S1.
PaxDbiQ9Y3S1.
PRIDEiQ9Y3S1.

PTM databases

PhosphoSiteiQ9Y3S1.

Expressioni

Tissue specificityi

Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon.3 Publications

Gene expression databases

BgeeiQ9Y3S1.
CleanExiHS_WNK2.
ExpressionAtlasiQ9Y3S1. baseline and differential.
GenevestigatoriQ9Y3S1.

Organism-specific databases

HPAiCAB017813.
HPA015555.
HPA016519.

Interactioni

Subunit structurei

Forms a complex with the phosphorylated form of STK39.By similarity

Protein-protein interaction databases

BioGridi122423. 9 interactions.
IntActiQ9Y3S1. 9 interactions.
MINTiMINT-7241250.
STRINGi9606.ENSP00000297954.

Structurei

3D structure databases

ProteinModelPortaliQ9Y3S1.
SMRiQ9Y3S1. Positions 184-545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 453259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074385.
HOGENOMiHOG000139922.
HOVERGENiHBG050345.
InParanoidiQ9Y3S1.
KOiK08867.
OMAiVKLPHPP.
PhylomeDBiQ9Y3S1.
TreeFamiTF315363.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q9Y3S1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGDGGRRDV PGTLMEPGRG AGPAGMAEPR AKAARPGPQR FLRRSVVESD
60 70 80 90 100
QEEPPGLEAA EAPGPQPPQP LQRRVLLLCK TRRLIAERAR GRPAAPAPAA
110 120 130 140 150
LVAQPGAPGA PADAGPEPVG TQEPGPDPIA AAVETAPAPD GGPREEAAAT
160 170 180 190 200
VRKEDEGAAE AKPEPGRTRR DEPEEEEDDE DDLKAVATSL DGRFLKFDIE
210 220 230 240 250
LGRGSFKTVY KGLDTETWVE VAWCELQDRK LTKLERQRFK EEAEMLKGLQ
260 270 280 290 300
HPNIVRFYDF WESSAKGKRC IVLVTELMTS GTLKTYLKRF KVMKPKVLRS
310 320 330 340 350
WCRQILKGLL FLHTRTPPII HRDLKCDNIF ITGPTGSVKI GDLGLATLKR
360 370 380 390 400
ASFAKSVIGT PEFMAPEMYE EHYDESVDVY AFGMCMLEMA TSEYPYSECQ
410 420 430 440 450
NAAQIYRKVT CGIKPASFEK VHDPEIKEII GECICKNKEE RYEIKDLLSH
460 470 480 490 500
AFFAEDTGVR VELAEEDHGR KSTIALRLWV EDPKKLKGKP KDNGAIEFTF
510 520 530 540 550
DLEKETPDEV AQEMIESGFF HESDVKIVAK SIRDRVALIQ WRRERIWPAL
560 570 580 590 600
QPKEQQDVGS PDKARGPPVP LQVQVTYHAQ AGQPGPPEPE EPEADQHLLP
610 620 630 640 650
PTLPTSATSL ASDSTFDSGQ GSTVYSDSQS SQQSVMLGSL ADAAPSPAQC
660 670 680 690 700
VCSPPVSEGP VLPQSLPSLG AYQQPTAAPG LPVGSVPAPA CPPSLQQHFP
710 720 730 740 750
DPAMSFAPVL PPPSTPMPTG PGQPAPPGQQ PPPLAQPTPL PQVLAPQPVV
760 770 780 790 800
PLQPVPPHLP PYLAPASQVG APAQLKPLQM PQAPLQPLAQ VPPQMPPIPV
810 820 830 840 850
VPPITPLAGI DGLPPALPDL PTATVPPVPP PQYFSPAVIL PSLAAPLPPA
860 870 880 890 900
SPALPLQAVK LPHPPGAPLA MPCRTIVPNA PATIPLLAVA PPGVAALSIH
910 920 930 940 950
SAVAQLPGQP VYPAAFPQMA PTDVPPSPHH TVQNMRATPP QPALPPQPTL
960 970 980 990 1000
PPQPVLPPQP TLPPQPVLPP QPTRPPQPVL PPQPMLPPQP VLPPQPALPV
1010 1020 1030 1040 1050
RPEPLQPHLP EQAAPAATPG SQILLGHPAP YAVDVAAQVP TVPVPPAAVL
1060 1070 1080 1090 1100
SPPLPEVLLP AAPELLPQFP SSLATVSASV QSVPTQTATL LPPANPPLPG
1110 1120 1130 1140 1150
GPGIASPCPT VQLTVEPVQE EQASQDKPPG LPQSCESYGG SDVTSGKELS
1160 1170 1180 1190 1200
DSCEGAFGGG RLEGRAARKH HRRSTRARSR QERASRPRLT ILNVCNTGDK
1210 1220 1230 1240 1250
MVECQLETHN HKMVTFKFDL DGDAPDEIAT YMVEHDFILQ AERETFIEQM
1260 1270 1280 1290 1300
KDVMDKAEDM LSEDTDADRG SDPGTSPPHL STCGLGTGEE SRQSQANAPV
1310 1320 1330 1340 1350
YQQNVLHTGK RWFIICPVAE HPAPEAPESS PPLPLSSLPP EASQGPCRGL
1360 1370 1380 1390 1400
TLPCLPWRRA ACGAVFLSLF SAESAQSKQP PDSAPYKDQL SSKEQPSFLA
1410 1420 1430 1440 1450
SQQLLSQAGP SNPPGAPPAP LAPSSPPVTA LPQDGAAPAT STMPEPASGT
1460 1470 1480 1490 1500
ASQAGGPGTP QGLTSELETS QPLAETHEAP LAVQPLVVGL APCTPAPEAA
1510 1520 1530 1540 1550
STRDASAPRE PLPPPAPEPS PHSGTPQPAL GQPAPLLPAA VGAVSLATSQ
1560 1570 1580 1590 1600
LPSPPLGPTV PPQPPSALES DGEGPPPRVG FVDSTIKSLD EKLRTLLYQE
1610 1620 1630 1640 1650
HVPTSSASAG TPVEVGDRDF TLEPLRGDQP RSEVCGGDLA LPPVPKEAVS
1660 1670 1680 1690 1700
GRVQLPQPLV EKSELAPTRG AVMEQGTSSS MTAESSPRSM LGYDRDGRQV
1710 1720 1730 1740 1750
ASDSHVVPSV PQDVPAFVRP ARVEPTDRDG GEAGESSAEP PPSDMGTVGG
1760 1770 1780 1790 1800
QASHPQTLGA RALGSPRKRP EQQDVSSPAK TVGRFSVVST QDEWTLASPH
1810 1820 1830 1840 1850
SLRYSAPPDV YLDEAPSSPD VKLAVRRAQT ASSIEVGVGE PVSSDSGDEG
1860 1870 1880 1890 1900
PRARPPVQKQ ASLPVSGSVA GDFVKKATAF LQRPSRAGSL GPETPSRVGM
1910 1920 1930 1940 1950
KVPTISVTSF HSQSSYISSD NDSELEDADI KKELQSLREK HLKEISELQS
1960 1970 1980 1990 2000
QQKQEIEALY RRLGKPLPPN VGFFHTAPPT GRRRKTSKSK LKAGKLLNPL
2010 2020 2030 2040 2050
VRQLKVVASS TGHLADSSRG PPAKDPAQAS VGLTADSTGL SGKAVQTQQP
2060 2070 2080 2090 2100
CSVRASLSSD ICSGLASDGG GARGQGWTVY HPTSERVTYK SSSKPRARFL
2110 2120 2130 2140 2150
SGPVSVSIWS ALKRLCLGKE HSSRSSTSSL APGPEPGPQP ALHVQAQVNN
2160 2170 2180 2190 2200
SNNKKGTFTD DLHKLVDEWT SKTVGAAQLK PTLNQLKQTQ KLQDMEAQAG
2210 2220 2230 2240 2250
WAAPGEARAM TAPRAGVGMP RLPPAPGPLS TTVIPGAAPT LSVPTPDGAL
2260 2270 2280 2290
GTARRNQVWF GLRVPPTACC GHSTQPRGGQ RVGSKTASFA ASDPVRS
Length:2,297
Mass (Da):242,676
Last modified:February 2, 2004 - v4
Checksum:i33D2EF2BFA6A1BD2
GO
Isoform 21 Publication (identifier: Q9Y3S1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1345-1381: Missing.
     2247-2261: DGALGTARRNQVWFG → GSCGPRAVSTPTSYT
     2262-2297: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:2,224
Mass (Da):234,948
Checksum:iBB5BC60D70A6E6D7
GO
Isoform 31 Publication (identifier: Q9Y3S1-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: Missing.
     680-731: Missing.
     843-845: LAA → RTR
     846-2297: Missing.

Note: Incomplete sequence.1 Publication

Show »
Length:779
Mass (Da):84,868
Checksum:iCC1BEDD3568635CD
GO
Isoform 41 Publication (identifier: Q9Y3S1-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2248-2254: GALGTAR → PESEKPD
     2255-2297: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:2,254
Mass (Da):238,279
Checksum:i8C4E5F1DB7EAD99A
GO

Sequence cautioni

The sequence AAH37965.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti828 – 8281V → M.
Corresponds to variant rs10761203 [ dbSNP | Ensembl ].
VAR_057114
Natural varianti974 – 9741R → L.
Corresponds to variant rs10114908 [ dbSNP | Ensembl ].
VAR_059773

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1414Missing in isoform 3. 1 PublicationVSP_050639Add
BLAST
Alternative sequencei680 – 73152Missing in isoform 3. 1 PublicationVSP_050640Add
BLAST
Alternative sequencei843 – 8453LAA → RTR in isoform 3. 1 PublicationVSP_050641
Alternative sequencei846 – 22971452Missing in isoform 3. 1 PublicationVSP_050642Add
BLAST
Alternative sequencei1345 – 138137Missing in isoform 2. 1 PublicationVSP_050643Add
BLAST
Alternative sequencei2247 – 226115DGALG…QVWFG → GSCGPRAVSTPTSYT in isoform 2. 1 PublicationVSP_050644Add
BLAST
Alternative sequencei2248 – 22547GALGTAR → PESEKPD in isoform 4. 1 PublicationVSP_050645
Alternative sequencei2255 – 229743Missing in isoform 4. 1 PublicationVSP_050646Add
BLAST
Alternative sequencei2262 – 229736Missing in isoform 2. 1 PublicationVSP_050647Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242724 mRNA. Translation: CAB44308.5.
AL390760, AL354991, AL583839 Genomic DNA. Translation: CAH73069.3.
AL390760, AL354991, AL583839 Genomic DNA. Translation: CAH73070.2.
AL583839, AL354991, AL390760 Genomic DNA. Translation: CAI12344.3.
AL583839, AL354991, AL390760 Genomic DNA. Translation: CAI12345.2.
AL354991, AL390760, AL583839 Genomic DNA. Translation: CAI14449.3.
AL354991, AL390760, AL583839 Genomic DNA. Translation: CAI14450.2.
AB044546 mRNA. Translation: BAB18648.1.
AB051547 mRNA. Translation: BAB21851.2.
BC037965 mRNA. Translation: AAH37965.1. Different initiation.
CCDSiCCDS75858.1. [Q9Y3S1-1]
RefSeqiNP_001269323.1. NM_001282394.1. [Q9Y3S1-1]
XP_005252197.1. XM_005252140.1. [Q9Y3S1-2]
UniGeneiHs.654856.

Genome annotation databases

EnsembliENST00000297954; ENSP00000297954; ENSG00000165238. [Q9Y3S1-1]
GeneIDi65268.
KEGGihsa:65268.
UCSCiuc004ati.1. human. [Q9Y3S1-1]
uc011lud.1. human. [Q9Y3S1-2]

Polymorphism databases

DMDMi41688799.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242724 mRNA. Translation: CAB44308.5 .
AL390760 , AL354991 , AL583839 Genomic DNA. Translation: CAH73069.3 .
AL390760 , AL354991 , AL583839 Genomic DNA. Translation: CAH73070.2 .
AL583839 , AL354991 , AL390760 Genomic DNA. Translation: CAI12344.3 .
AL583839 , AL354991 , AL390760 Genomic DNA. Translation: CAI12345.2 .
AL354991 , AL390760 , AL583839 Genomic DNA. Translation: CAI14449.3 .
AL354991 , AL390760 , AL583839 Genomic DNA. Translation: CAI14450.2 .
AB044546 mRNA. Translation: BAB18648.1 .
AB051547 mRNA. Translation: BAB21851.2 .
BC037965 mRNA. Translation: AAH37965.1 . Different initiation.
CCDSi CCDS75858.1. [Q9Y3S1-1 ]
RefSeqi NP_001269323.1. NM_001282394.1. [Q9Y3S1-1 ]
XP_005252197.1. XM_005252140.1. [Q9Y3S1-2 ]
UniGenei Hs.654856.

3D structure databases

ProteinModelPortali Q9Y3S1.
SMRi Q9Y3S1. Positions 184-545.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122423. 9 interactions.
IntActi Q9Y3S1. 9 interactions.
MINTi MINT-7241250.
STRINGi 9606.ENSP00000297954.

Chemistry

BindingDBi Q9Y3S1.
ChEMBLi CHEMBL5639.
GuidetoPHARMACOLOGYi 2281.

PTM databases

PhosphoSitei Q9Y3S1.

Polymorphism databases

DMDMi 41688799.

Proteomic databases

MaxQBi Q9Y3S1.
PaxDbi Q9Y3S1.
PRIDEi Q9Y3S1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000297954 ; ENSP00000297954 ; ENSG00000165238 . [Q9Y3S1-1 ]
GeneIDi 65268.
KEGGi hsa:65268.
UCSCi uc004ati.1. human. [Q9Y3S1-1 ]
uc011lud.1. human. [Q9Y3S1-2 ]

Organism-specific databases

CTDi 65268.
GeneCardsi GC09P095947.
HGNCi HGNC:14542. WNK2.
HPAi CAB017813.
HPA015555.
HPA016519.
MIMi 606249. gene.
neXtProti NX_Q9Y3S1.
PharmGKBi PA33783.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074385.
HOGENOMi HOG000139922.
HOVERGENi HBG050345.
InParanoidi Q9Y3S1.
KOi K08867.
OMAi VKLPHPP.
PhylomeDBi Q9Y3S1.
TreeFami TF315363.

Enzyme and pathway databases

Reactomei REACT_160189. Stimuli-sensing channels.
SignaLinki Q9Y3S1.

Miscellaneous databases

ChiTaRSi WNK2. human.
GeneWikii WNK2.
GenomeRNAii 65268.
NextBioi 67408.
PROi Q9Y3S1.
SOURCEi Search...

Gene expression databases

Bgeei Q9Y3S1.
CleanExi HS_WNK2.
ExpressionAtlasi Q9Y3S1. baseline and differential.
Genevestigatori Q9Y3S1.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "WNK kinases, a novel protein kinase subfamily in multi-cellular organisms."
    Verissimo F., Jordan P.
    Oncogene 20:5562-5569(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHROMOSOMAL LOCATION.
    Tissue: Colon epithelium1 Publication.
  2. Jordan P.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Molecular basis of T cell-mediated recognition of pancreatic cancer cells."
    Ito M., Shichijo S., Tsuda N., Ochi M., Harashima N., Saito N., Itoh K.
    Cancer Res. 61:2038-2046(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-845 (ISOFORM 3).
    Tissue: Pancreatic cancer1 Publication.
  5. "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O.
    DNA Res. 7:347-355(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-2297 (ISOFORM 2).
    Tissue: BrainImported.
  6. Ohara O., Nagase T., Kikuno R.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1609-2297 (ISOFORM 4).
    Tissue: Lung1 Publication.
  8. "Protein kinase WNK2 inhibits cell proliferation by negatively modulating the activation of MEK1/ERK1/2."
    Moniz S., Verissimo F., Matos P., Brazao R., Silva E., Kotelevets L., Kotevelets L., Chastre E., Gespach C., Jordan P.
    Oncogene 26:6071-6081(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION.
  9. "WNK2 modulates MEK1 activity through the Rho GTPase pathway."
    Moniz S., Matos P., Jordan P.
    Cell. Signal. 20:1762-1768(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1817 AND SER-1818, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1817 AND SER-1818, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  13. Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-45; SER-560; SER-1150; SER-1262; SER-1588; SER-1685; SER-1736; SER-1817; SER-1818; SER-1862 AND SER-2067.
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1862, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiWNK2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3S1
Secondary accession number(s): Q5VWF1
, Q5VWF2, Q8IY36, Q9C0A3, Q9H3P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: November 26, 2014
This is version 135 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3