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Q9Y3S1

- WNK2_HUMAN

UniProt

Q9Y3S1 - WNK2_HUMAN

Protein

Serine/threonine-protein kinase WNK2

Gene

WNK2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 4 (02 Feb 2004)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activation requires autophosphorylation of Ser-356. Phosphorylation of Ser-352 also promotes increased activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei207 – 2071ATPBy similarityPROSITE-ProRule annotation
    Active sitei323 – 3231Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi201 – 2099ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. intracellular signal transduction Source: UniProtKB
    2. negative regulation of cell proliferation Source: UniProtKB
    3. negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
    4. positive regulation of sodium ion transmembrane transporter activity Source: UniProtKB
    5. protein autophosphorylation Source: UniProtKB
    6. protein phosphorylation Source: UniProtKB
    7. regulation of ion homeostasis Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_160189. Stimuli-sensing channels.
    SignaLinkiQ9Y3S1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase WNK2 (EC:2.7.11.1)
    Alternative name(s):
    Antigen NY-CO-43
    Protein kinase lysine-deficient 2
    Protein kinase with no lysine 2
    Serologically defined colon cancer antigen 43
    Gene namesi
    Name:WNK2
    Synonyms:KIAA1760, PRKWNK2, SDCCAG43
    ORF Names:P/OKcl.13
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:14542. WNK2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33783.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22972297Serine/threonine-protein kinase WNK2PRO_0000086822Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei45 – 451Phosphoserine2 Publications
    Modified residuei352 – 3521Phosphoserine; by autocatalysisBy similarity
    Modified residuei356 – 3561Phosphoserine; by autocatalysisBy similarity
    Modified residuei560 – 5601Phosphoserine2 Publications
    Modified residuei1150 – 11501Phosphoserine2 Publications
    Modified residuei1262 – 12621Phosphoserine2 Publications
    Modified residuei1588 – 15881Phosphoserine2 Publications
    Modified residuei1685 – 16851Phosphoserine2 Publications
    Modified residuei1736 – 17361Phosphoserine2 Publications
    Modified residuei1817 – 18171Phosphoserine4 Publications
    Modified residuei1818 – 18181Phosphoserine4 Publications
    Modified residuei1862 – 18621Phosphoserine3 Publications
    Modified residuei1889 – 18891PhosphoserineBy similarity
    Modified residuei2067 – 20671Phosphoserine2 Publications

    Post-translational modificationi

    Autophosphorylated.5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Y3S1.
    PaxDbiQ9Y3S1.
    PRIDEiQ9Y3S1.

    PTM databases

    PhosphoSiteiQ9Y3S1.

    Expressioni

    Tissue specificityi

    Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon.3 Publications

    Gene expression databases

    ArrayExpressiQ9Y3S1.
    BgeeiQ9Y3S1.
    CleanExiHS_WNK2.
    GenevestigatoriQ9Y3S1.

    Organism-specific databases

    HPAiCAB017813.
    HPA015555.
    HPA016519.

    Interactioni

    Subunit structurei

    Forms a complex with the phosphorylated form of STK39.By similarity

    Protein-protein interaction databases

    BioGridi122423. 7 interactions.
    IntActiQ9Y3S1. 9 interactions.
    MINTiMINT-7241250.
    STRINGi9606.ENSP00000297954.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y3S1.
    SMRiQ9Y3S1. Positions 184-545.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini195 – 453259Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000139922.
    HOVERGENiHBG050345.
    InParanoidiQ9Y3S1.
    KOiK08867.
    OMAiVKLPHPP.
    PhylomeDBiQ9Y3S1.
    TreeFamiTF315363.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR024678. Kinase_OSR1/WNK_CCT.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF12202. OSR1_C. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q9Y3S1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDGDGGRRDV PGTLMEPGRG AGPAGMAEPR AKAARPGPQR FLRRSVVESD     50
    QEEPPGLEAA EAPGPQPPQP LQRRVLLLCK TRRLIAERAR GRPAAPAPAA 100
    LVAQPGAPGA PADAGPEPVG TQEPGPDPIA AAVETAPAPD GGPREEAAAT 150
    VRKEDEGAAE AKPEPGRTRR DEPEEEEDDE DDLKAVATSL DGRFLKFDIE 200
    LGRGSFKTVY KGLDTETWVE VAWCELQDRK LTKLERQRFK EEAEMLKGLQ 250
    HPNIVRFYDF WESSAKGKRC IVLVTELMTS GTLKTYLKRF KVMKPKVLRS 300
    WCRQILKGLL FLHTRTPPII HRDLKCDNIF ITGPTGSVKI GDLGLATLKR 350
    ASFAKSVIGT PEFMAPEMYE EHYDESVDVY AFGMCMLEMA TSEYPYSECQ 400
    NAAQIYRKVT CGIKPASFEK VHDPEIKEII GECICKNKEE RYEIKDLLSH 450
    AFFAEDTGVR VELAEEDHGR KSTIALRLWV EDPKKLKGKP KDNGAIEFTF 500
    DLEKETPDEV AQEMIESGFF HESDVKIVAK SIRDRVALIQ WRRERIWPAL 550
    QPKEQQDVGS PDKARGPPVP LQVQVTYHAQ AGQPGPPEPE EPEADQHLLP 600
    PTLPTSATSL ASDSTFDSGQ GSTVYSDSQS SQQSVMLGSL ADAAPSPAQC 650
    VCSPPVSEGP VLPQSLPSLG AYQQPTAAPG LPVGSVPAPA CPPSLQQHFP 700
    DPAMSFAPVL PPPSTPMPTG PGQPAPPGQQ PPPLAQPTPL PQVLAPQPVV 750
    PLQPVPPHLP PYLAPASQVG APAQLKPLQM PQAPLQPLAQ VPPQMPPIPV 800
    VPPITPLAGI DGLPPALPDL PTATVPPVPP PQYFSPAVIL PSLAAPLPPA 850
    SPALPLQAVK LPHPPGAPLA MPCRTIVPNA PATIPLLAVA PPGVAALSIH 900
    SAVAQLPGQP VYPAAFPQMA PTDVPPSPHH TVQNMRATPP QPALPPQPTL 950
    PPQPVLPPQP TLPPQPVLPP QPTRPPQPVL PPQPMLPPQP VLPPQPALPV 1000
    RPEPLQPHLP EQAAPAATPG SQILLGHPAP YAVDVAAQVP TVPVPPAAVL 1050
    SPPLPEVLLP AAPELLPQFP SSLATVSASV QSVPTQTATL LPPANPPLPG 1100
    GPGIASPCPT VQLTVEPVQE EQASQDKPPG LPQSCESYGG SDVTSGKELS 1150
    DSCEGAFGGG RLEGRAARKH HRRSTRARSR QERASRPRLT ILNVCNTGDK 1200
    MVECQLETHN HKMVTFKFDL DGDAPDEIAT YMVEHDFILQ AERETFIEQM 1250
    KDVMDKAEDM LSEDTDADRG SDPGTSPPHL STCGLGTGEE SRQSQANAPV 1300
    YQQNVLHTGK RWFIICPVAE HPAPEAPESS PPLPLSSLPP EASQGPCRGL 1350
    TLPCLPWRRA ACGAVFLSLF SAESAQSKQP PDSAPYKDQL SSKEQPSFLA 1400
    SQQLLSQAGP SNPPGAPPAP LAPSSPPVTA LPQDGAAPAT STMPEPASGT 1450
    ASQAGGPGTP QGLTSELETS QPLAETHEAP LAVQPLVVGL APCTPAPEAA 1500
    STRDASAPRE PLPPPAPEPS PHSGTPQPAL GQPAPLLPAA VGAVSLATSQ 1550
    LPSPPLGPTV PPQPPSALES DGEGPPPRVG FVDSTIKSLD EKLRTLLYQE 1600
    HVPTSSASAG TPVEVGDRDF TLEPLRGDQP RSEVCGGDLA LPPVPKEAVS 1650
    GRVQLPQPLV EKSELAPTRG AVMEQGTSSS MTAESSPRSM LGYDRDGRQV 1700
    ASDSHVVPSV PQDVPAFVRP ARVEPTDRDG GEAGESSAEP PPSDMGTVGG 1750
    QASHPQTLGA RALGSPRKRP EQQDVSSPAK TVGRFSVVST QDEWTLASPH 1800
    SLRYSAPPDV YLDEAPSSPD VKLAVRRAQT ASSIEVGVGE PVSSDSGDEG 1850
    PRARPPVQKQ ASLPVSGSVA GDFVKKATAF LQRPSRAGSL GPETPSRVGM 1900
    KVPTISVTSF HSQSSYISSD NDSELEDADI KKELQSLREK HLKEISELQS 1950
    QQKQEIEALY RRLGKPLPPN VGFFHTAPPT GRRRKTSKSK LKAGKLLNPL 2000
    VRQLKVVASS TGHLADSSRG PPAKDPAQAS VGLTADSTGL SGKAVQTQQP 2050
    CSVRASLSSD ICSGLASDGG GARGQGWTVY HPTSERVTYK SSSKPRARFL 2100
    SGPVSVSIWS ALKRLCLGKE HSSRSSTSSL APGPEPGPQP ALHVQAQVNN 2150
    SNNKKGTFTD DLHKLVDEWT SKTVGAAQLK PTLNQLKQTQ KLQDMEAQAG 2200
    WAAPGEARAM TAPRAGVGMP RLPPAPGPLS TTVIPGAAPT LSVPTPDGAL 2250
    GTARRNQVWF GLRVPPTACC GHSTQPRGGQ RVGSKTASFA ASDPVRS 2297
    Length:2,297
    Mass (Da):242,676
    Last modified:February 2, 2004 - v4
    Checksum:i33D2EF2BFA6A1BD2
    GO
    Isoform 21 Publication (identifier: Q9Y3S1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1345-1381: Missing.
         2247-2261: DGALGTARRNQVWFG → GSCGPRAVSTPTSYT
         2262-2297: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:2,224
    Mass (Da):234,948
    Checksum:iBB5BC60D70A6E6D7
    GO
    Isoform 31 Publication (identifier: Q9Y3S1-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-14: Missing.
         680-731: Missing.
         843-845: LAA → RTR
         846-2297: Missing.

    Note: Incomplete sequence.1 Publication

    Show »
    Length:779
    Mass (Da):84,868
    Checksum:iCC1BEDD3568635CD
    GO
    Isoform 41 Publication (identifier: Q9Y3S1-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2248-2254: GALGTAR → PESEKPD
         2255-2297: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:2,254
    Mass (Da):238,279
    Checksum:i8C4E5F1DB7EAD99A
    GO

    Sequence cautioni

    The sequence AAH37965.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti828 – 8281V → M.
    Corresponds to variant rs10761203 [ dbSNP | Ensembl ].
    VAR_057114
    Natural varianti974 – 9741R → L.
    Corresponds to variant rs10114908 [ dbSNP | Ensembl ].
    VAR_059773

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1414Missing in isoform 3. 1 PublicationVSP_050639Add
    BLAST
    Alternative sequencei680 – 73152Missing in isoform 3. 1 PublicationVSP_050640Add
    BLAST
    Alternative sequencei843 – 8453LAA → RTR in isoform 3. 1 PublicationVSP_050641
    Alternative sequencei846 – 22971452Missing in isoform 3. 1 PublicationVSP_050642Add
    BLAST
    Alternative sequencei1345 – 138137Missing in isoform 2. 1 PublicationVSP_050643Add
    BLAST
    Alternative sequencei2247 – 226115DGALG…QVWFG → GSCGPRAVSTPTSYT in isoform 2. 1 PublicationVSP_050644Add
    BLAST
    Alternative sequencei2248 – 22547GALGTAR → PESEKPD in isoform 4. 1 PublicationVSP_050645
    Alternative sequencei2255 – 229743Missing in isoform 4. 1 PublicationVSP_050646Add
    BLAST
    Alternative sequencei2262 – 229736Missing in isoform 2. 1 PublicationVSP_050647Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ242724 mRNA. Translation: CAB44308.5.
    AL390760, AL354991, AL583839 Genomic DNA. Translation: CAH73069.3.
    AL390760, AL354991, AL583839 Genomic DNA. Translation: CAH73070.2.
    AL583839, AL354991, AL390760 Genomic DNA. Translation: CAI12344.3.
    AL583839, AL354991, AL390760 Genomic DNA. Translation: CAI12345.2.
    AL354991, AL390760, AL583839 Genomic DNA. Translation: CAI14449.3.
    AL354991, AL390760, AL583839 Genomic DNA. Translation: CAI14450.2.
    AB044546 mRNA. Translation: BAB18648.1.
    AB051547 mRNA. Translation: BAB21851.2.
    BC037965 mRNA. Translation: AAH37965.1. Different initiation.
    RefSeqiNP_001269323.1. NM_001282394.1. [Q9Y3S1-1]
    XP_005252197.1. XM_005252140.1. [Q9Y3S1-2]
    UniGeneiHs.654856.

    Genome annotation databases

    EnsembliENST00000297954; ENSP00000297954; ENSG00000165238. [Q9Y3S1-1]
    GeneIDi65268.
    KEGGihsa:65268.
    UCSCiuc004ati.1. human. [Q9Y3S1-1]
    uc011lud.1. human. [Q9Y3S1-2]

    Polymorphism databases

    DMDMi41688799.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ242724 mRNA. Translation: CAB44308.5 .
    AL390760 , AL354991 , AL583839 Genomic DNA. Translation: CAH73069.3 .
    AL390760 , AL354991 , AL583839 Genomic DNA. Translation: CAH73070.2 .
    AL583839 , AL354991 , AL390760 Genomic DNA. Translation: CAI12344.3 .
    AL583839 , AL354991 , AL390760 Genomic DNA. Translation: CAI12345.2 .
    AL354991 , AL390760 , AL583839 Genomic DNA. Translation: CAI14449.3 .
    AL354991 , AL390760 , AL583839 Genomic DNA. Translation: CAI14450.2 .
    AB044546 mRNA. Translation: BAB18648.1 .
    AB051547 mRNA. Translation: BAB21851.2 .
    BC037965 mRNA. Translation: AAH37965.1 . Different initiation.
    RefSeqi NP_001269323.1. NM_001282394.1. [Q9Y3S1-1 ]
    XP_005252197.1. XM_005252140.1. [Q9Y3S1-2 ]
    UniGenei Hs.654856.

    3D structure databases

    ProteinModelPortali Q9Y3S1.
    SMRi Q9Y3S1. Positions 184-545.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122423. 7 interactions.
    IntActi Q9Y3S1. 9 interactions.
    MINTi MINT-7241250.
    STRINGi 9606.ENSP00000297954.

    Chemistry

    BindingDBi Q9Y3S1.
    ChEMBLi CHEMBL5639.
    GuidetoPHARMACOLOGYi 2281.

    PTM databases

    PhosphoSitei Q9Y3S1.

    Polymorphism databases

    DMDMi 41688799.

    Proteomic databases

    MaxQBi Q9Y3S1.
    PaxDbi Q9Y3S1.
    PRIDEi Q9Y3S1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000297954 ; ENSP00000297954 ; ENSG00000165238 . [Q9Y3S1-1 ]
    GeneIDi 65268.
    KEGGi hsa:65268.
    UCSCi uc004ati.1. human. [Q9Y3S1-1 ]
    uc011lud.1. human. [Q9Y3S1-2 ]

    Organism-specific databases

    CTDi 65268.
    GeneCardsi GC09P095947.
    HGNCi HGNC:14542. WNK2.
    HPAi CAB017813.
    HPA015555.
    HPA016519.
    MIMi 606249. gene.
    neXtProti NX_Q9Y3S1.
    PharmGKBi PA33783.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000139922.
    HOVERGENi HBG050345.
    InParanoidi Q9Y3S1.
    KOi K08867.
    OMAi VKLPHPP.
    PhylomeDBi Q9Y3S1.
    TreeFami TF315363.

    Enzyme and pathway databases

    Reactomei REACT_160189. Stimuli-sensing channels.
    SignaLinki Q9Y3S1.

    Miscellaneous databases

    GeneWikii WNK2.
    GenomeRNAii 65268.
    NextBioi 67408.
    PROi Q9Y3S1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y3S1.
    Bgeei Q9Y3S1.
    CleanExi HS_WNK2.
    Genevestigatori Q9Y3S1.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR024678. Kinase_OSR1/WNK_CCT.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF12202. OSR1_C. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "WNK kinases, a novel protein kinase subfamily in multi-cellular organisms."
      Verissimo F., Jordan P.
      Oncogene 20:5562-5569(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHROMOSOMAL LOCATION.
      Tissue: Colon epithelium1 Publication.
    2. Jordan P.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Molecular basis of T cell-mediated recognition of pancreatic cancer cells."
      Ito M., Shichijo S., Tsuda N., Ochi M., Harashima N., Saito N., Itoh K.
      Cancer Res. 61:2038-2046(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-845 (ISOFORM 3).
      Tissue: Pancreatic cancer1 Publication.
    5. "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O.
      DNA Res. 7:347-355(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-2297 (ISOFORM 2).
      Tissue: BrainImported.
    6. Ohara O., Nagase T., Kikuno R.
      Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1609-2297 (ISOFORM 4).
      Tissue: Lung1 Publication.
    8. "Protein kinase WNK2 inhibits cell proliferation by negatively modulating the activation of MEK1/ERK1/2."
      Moniz S., Verissimo F., Matos P., Brazao R., Silva E., Kotelevets L., Kotevelets L., Chastre E., Gespach C., Jordan P.
      Oncogene 26:6071-6081(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION.
    9. "WNK2 modulates MEK1 activity through the Rho GTPase pathway."
      Moniz S., Matos P., Jordan P.
      Cell. Signal. 20:1762-1768(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1817 AND SER-1818, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1817 AND SER-1818, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    13. Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-45; SER-560; SER-1150; SER-1262; SER-1588; SER-1685; SER-1736; SER-1817; SER-1818; SER-1862 AND SER-2067.
    14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1862, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiWNK2_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y3S1
    Secondary accession number(s): Q5VWF1
    , Q5VWF2, Q8IY36, Q9C0A3, Q9H3P4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: February 2, 2004
    Last modified: October 1, 2014
    This is version 133 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3