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Q9Y3Q7 (ADA18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 18

Short name=ADAM 18
Alternative name(s):
Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
Short name=tMDC III
Gene names
Name:ADAM18
Synonyms:TMDC3
ORF Names:UNQ858/PRO1867
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed specifically in testis.

Domain

A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding By similarity.

Post-translational modification

The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y3Q7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y3Q7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     197-220: Missing.
Isoform 3 (identifier: Q9Y3Q7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     175-182: IKNLSKLL → VTVIILML
     183-739: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Propeptide17 – 184168 Potential
PRO_0000029096
Chain185 – 739555Disintegrin and metalloproteinase domain-containing protein 18
PRO_0000029097

Regions

Topological domain177 – 687511Extracellular Potential
Transmembrane688 – 70821Helical; Potential
Topological domain709 – 73931Cytoplasmic Potential
Domain184 – 381198Peptidase M12B
Domain390 – 47990Disintegrin
Domain620 – 65435EGF-like
Compositional bias480 – 622143Cys-rich

Amino acid modifications

Glycosylation361N-linked (GlcNAc...) Potential
Glycosylation761N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Potential
Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation3591N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation5611N-linked (GlcNAc...) Potential
Glycosylation6111N-linked (GlcNAc...) Potential
Glycosylation6251N-linked (GlcNAc...) Potential
Disulfide bond293 ↔ 376 By similarity
Disulfide bond335 ↔ 360 By similarity
Disulfide bond337 ↔ 342 By similarity
Disulfide bond450 ↔ 471 By similarity
Disulfide bond624 ↔ 636 By similarity
Disulfide bond630 ↔ 642 By similarity
Disulfide bond644 ↔ 653 By similarity

Natural variations

Alternative sequence175 – 1828IKNLSKLL → VTVIILML in isoform 3.
VSP_043285
Alternative sequence183 – 739557Missing in isoform 3.
VSP_043286
Alternative sequence197 – 22024Missing in isoform 2.
VSP_012033
Natural variant1701P → S in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066312
Natural variant2121V → F.
Corresponds to variant rs10093794 [ dbSNP | Ensembl ].
VAR_051588
Natural variant2841V → G in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066313
Natural variant3441M → I in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066314
Natural variant3621M → K in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066315
Natural variant5361S → L in a cutaneous metastatic melanoma sample; somatic mutation. Ref.6
VAR_066316

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 2D8BE9A975072CDD

FASTA73982,856
        10         20         30         40         50         60 
MFLLLALLTE LGRLQAHEGS EGIFLHVTVP RKIKSNDSEV SERKMIYIIT IDGQPYTLHL 

        70         80         90        100        110        120 
GKQSFLPQNF LVYTYNETGS LHSVSPYFMM HCHYQGYAAE FPNSFVTLSI CSGLRGFLQF 

       130        140        150        160        170        180 
ENISYGIEPV ESSARFEHII YQMKNNDPNV SILAVNYSHI WQKDQPYKVP LNSQIKNLSK 

       190        200        210        220        230        240 
LLPQYLEIYI IVEKALYDYM GSEMMAVTQK IVQVIGLVNT MFTQFKLTVI LSSLELWSNE 

       250        260        270        280        290        300 
NQISTSGDAD DILQRFLAWK RDYLILRPHD IAYLLVYRKH PKYVGATFPG TVCNKSYDAG 

       310        320        330        340        350        360 
IAMYPDAIGL EGFSVIIAQL LGLNVGLTYD DITQCFCLRA TCIMNHEAVS ASGRKIFSNC 

       370        380        390        400        410        420 
SMHDYRYFVS KFETKCLQKL SNLQPLHQNQ PVCGNGILES NEECDCGNKN ECQFKKCCDY 

       430        440        450        460        470        480 
NTCKLKGSVK CGSGPCCTSK CELSIAGTPC RKSIDPECDF TEYCNGTSSN CVPDTYALNG 

       490        500        510        520        530        540 
RLCKLGTAYC YNGQCQTTDN QCAKIFGKGA QGAPFACFKE VNSLHERSEN CGFKNSQPLP 

       550        560        570        580        590        600 
CERKDVLCGK LACVQPHKNA NKSDAQSTVY SYIQDHVCVS IATGSSMRSD GTDNAYVADG 

       610        620        630        640        650        660 
TMCGPEMYCV NKTCRKVHLM GYNCNATTKC KGKGICNNFG NCQCFPGHRP PDCKFQFGSP 

       670        680        690        700        710        720 
GGSIDDGNFQ KSGDFYTEKG YNTHWNNWFI LSFCIFLPFF IVFTTVIFKR NEISKSCNRE 

       730 
NAEYNRNSSV VSESDDVGH 

« Hide

Isoform 2 [UniParc].

Checksum: EF8EB71EBA6AB9DB
Show »

FASTA71580,183
Isoform 3 [UniParc].

Checksum: 8B524D83BC944C8B
Show »

FASTA18220,853

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the human tMDC III sperm surface protein transcript."
Hall L., Frayne J., Dimsey E.A.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[4]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Testis.
[6]"Analysis of the disintegrin-metalloproteinases family reveals ADAM29 and ADAM7 are often mutated in melanoma."
Wei X., Moncada-Pazos A., Cal S., Soria-Valles C., Gartner J., Rudloff U., Lin J.C., Rosenberg S.A., Lopez-Otin C., Samuels Y.
Hum. Mutat. 32:E2148-E2175(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS SER-170; GLY-284; ILE-344; LYS-362 AND LEU-536.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ133004 mRNA. Translation: CAB40812.1.
AY358321 mRNA. Translation: AAQ88687.1.
AK313961 mRNA. Translation: BAG36677.1.
AC109633 Genomic DNA. No translation available.
AC136365 Genomic DNA. No translation available.
BC034624 mRNA. Translation: AAH34624.1.
BC070279 mRNA. Translation: AAH70279.1.
BC121045 mRNA. Translation: AAI21046.1.
CCDSCCDS55225.1. [Q9Y3Q7-3]
CCDS6113.1. [Q9Y3Q7-1]
RefSeqNP_001177885.1. NM_001190956.1. [Q9Y3Q7-3]
NP_055052.1. NM_014237.2. [Q9Y3Q7-1]
UniGeneHs.127930.

3D structure databases

ProteinModelPortalQ9Y3Q7.
SMRQ9Y3Q7. Positions 184-657.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000265707.

Protein family/group databases

MEROPSM12.957.

PTM databases

PhosphoSiteQ9Y3Q7.

Polymorphism databases

DMDM20137582.

Proteomic databases

PaxDbQ9Y3Q7.
PRIDEQ9Y3Q7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265707; ENSP00000265707; ENSG00000168619. [Q9Y3Q7-1]
ENST00000379866; ENSP00000369195; ENSG00000168619. [Q9Y3Q7-2]
ENST00000520772; ENSP00000429908; ENSG00000168619. [Q9Y3Q7-3]
GeneID8749.
KEGGhsa:8749.
UCSCuc003xnh.3. human. [Q9Y3Q7-3]
uc003xni.3. human. [Q9Y3Q7-1]
uc010lwx.3. human. [Q9Y3Q7-2]

Organism-specific databases

CTD8749.
GeneCardsGC08P039459.
HGNCHGNC:196. ADAM18.
HPAHPA027605.
neXtProtNX_Q9Y3Q7.
PharmGKBPA24513.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG329803.
HOGENOMHOG000230883.
HOVERGENHBG103628.
InParanoidQ9Y3Q7.
KOK16909.
OMADYMGSEM.
OrthoDBEOG7W1537.
PhylomeDBQ9Y3Q7.
TreeFamTF314733.

Gene expression databases

ArrayExpressQ9Y3Q7.
BgeeQ9Y3Q7.
CleanExHS_ADAM18.
GenevestigatorQ9Y3Q7.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMSSF57552. SSF57552. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSADAM18. human.
GeneWikiADAM18.
GenomeRNAi8749.
NextBio32825.
PROQ9Y3Q7.

Entry information

Entry nameADA18_HUMAN
AccessionPrimary (citable) accession number: Q9Y3Q7
Secondary accession number(s): B2R9Y0 expand/collapse secondary AC list , Q0VAI4, Q6IRW9, Q6UXJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM