Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Signaling threshold-regulating transmembrane adapter 1

Gene

SIT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.1 Publication

GO - Molecular functioni

  • kinase binding Source: UniProtKB
  • SH2 domain binding Source: HGNC

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • regulation of T cell activation Source: UniProtKB
  • signal transduction Source: ProtInc
  • T cell homeostasis Source: InterPro

Keywordsi

Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

SignaLinkiQ9Y3P8.

Names & Taxonomyi

Protein namesi
Recommended name:
Signaling threshold-regulating transmembrane adapter 1
Alternative name(s):
SHP2-interacting transmembrane adapter protein
Suppression-inducing transmembrane adapter 1
gp30/40
Gene namesi
Name:SIT1
Synonyms:SIT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000137078.8.
HGNCiHGNC:17710. SIT1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 40ExtracellularSequence analysisAdd BLAST16
Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
Topological domaini62 – 196CytoplasmicSequence analysisAdd BLAST135

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26N → Q: Abolishes glycosylation. 1 Publication1
Mutagenesisi90Y → F: Reduces interaction with GRB2. Abolishes interaction with GRB2; when associated with F-188. 1 Publication1
Mutagenesisi127Y → F: No effect on interaction with PTPN11 or GRB2. 1 Publication1
Mutagenesisi148Y → F: Reduces interaction with PTPN11, no effect on inhibition of NF-AT activation. 2 Publications1
Mutagenesisi169Y → F: Abolishes interaction with CSK and impairs inhibition of NF-AT activation. 1 Publication1
Mutagenesisi188Y → F: Reduces interaction with GRB2. Abolishes interaction with GRB2; when associated with F-90. 1 Publication1

Organism-specific databases

DisGeNETi27240.
OpenTargetsiENSG00000137078.
PharmGKBiPA142670914.

Polymorphism and mutation databases

BioMutaiSIT1.
DMDMi74753488.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000004515225 – 196Signaling threshold-regulating transmembrane adapter 1Add BLAST172

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi26N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi27InterchainCurated
Modified residuei80PhosphoserineCombined sources1
Modified residuei83PhosphoserineBy similarity1
Modified residuei90PhosphotyrosineCombined sources1 Publication1
Modified residuei102PhosphoserineCombined sources1
Modified residuei127Phosphotyrosine1 Publication1
Modified residuei144PhosphothreonineCombined sources1
Modified residuei148PhosphotyrosineCombined sources2 Publications1
Modified residuei169Phosphotyrosine1 Publication1
Modified residuei182PhosphoserineCombined sources1
Modified residuei188PhosphotyrosineCombined sources1 Publication1

Post-translational modificationi

Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Y3P8.
PaxDbiQ9Y3P8.
PeptideAtlasiQ9Y3P8.
PRIDEiQ9Y3P8.

PTM databases

iPTMnetiQ9Y3P8.
PhosphoSitePlusiQ9Y3P8.

Expressioni

Tissue specificityi

Specifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level). Expressed in T- and B-cell lymphoma.2 Publications

Gene expression databases

BgeeiENSG00000137078.
CleanExiHS_SIT1.
ExpressionAtlasiQ9Y3P8. baseline and differential.
GenevisibleiQ9Y3P8. HS.

Organism-specific databases

HPAiHPA018506.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. When phosphorylated, interacts with PTPN11/SHP2, GRB2 and CSK.2 Publications

GO - Molecular functioni

  • kinase binding Source: UniProtKB
  • SH2 domain binding Source: HGNC

Protein-protein interaction databases

BioGridi118088. 5 interactors.
IntActiQ9Y3P8. 1 interactor.
MINTiMINT-8020372.
STRINGi9606.ENSP00000259608.

Structurei

3D structure databases

ProteinModelPortaliQ9Y3P8.
SMRiQ9Y3P8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 93Interaction with GRB21 Publication4
Regioni146 – 151Interaction with PTPN116
Regioni169 – 172Interaction with CSK1 Publication4
Regioni188 – 191Interaction with GRB21 Publication4

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYYR. Eukaryota.
ENOG4111C3Q. LUCA.
GeneTreeiENSGT00390000016476.
HOGENOMiHOG000231524.
HOVERGENiHBG080311.
InParanoidiQ9Y3P8.
PhylomeDBiQ9Y3P8.
TreeFamiTF337816.

Family and domain databases

InterProiView protein in InterPro
IPR033269. Sit1.
PANTHERiPTHR15604. PTHR15604. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD358400. PD358400. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y3P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQADPRLRA VCLWTLTSAA MSRGDNCTDL LALGIPSITQ AWGLWVLLGA
60 70 80 90 100
VTLLFLISLA AHLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR
110 120 130 140 150
LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE
160 170 180 190
VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS
Length:196
Mass (Da):21,126
Last modified:November 1, 1999 - v1
Checksum:i2A0C48C9466F7F07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010059 mRNA. Translation: CAB41504.1.
AJ271888 Genomic DNA. Translation: CAC81313.1.
AK314758 mRNA. Translation: BAG37296.1.
AL357874 Genomic DNA. No translation available.
CH471071 Genomic DNA. Translation: EAW58370.1.
BC102029 mRNA. Translation: AAI02030.1.
BC104491 mRNA. Translation: AAI04492.1.
BC107484 mRNA. Translation: AAI07485.1.
CCDSiCCDS6582.1.
RefSeqiNP_055265.1. NM_014450.2.
UniGeneiHs.88012.

Genome annotation databases

EnsembliENST00000259608; ENSP00000259608; ENSG00000137078.
GeneIDi27240.
KEGGihsa:27240.
UCSCiuc003zxe.3. human.

Similar proteinsi

Entry informationi

Entry nameiSIT1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3P8
Secondary accession number(s): B2RBP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1999
Last modified: September 27, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

In contrast to its orthologs it harbors a signal sequence.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot