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Protein

Protein chibby homolog 1

Gene

CBY1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the Wnt/Wingless pathway by binding to beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.2 Publications

GO - Molecular functioni

  1. beta-catenin binding Source: UniProtKB
  2. identical protein binding Source: UniProtKB

GO - Biological processi

  1. cardiac muscle cell differentiation Source: UniProtKB
  2. fat cell differentiation Source: UniProtKB
  3. negative regulation of transcription, DNA-templated Source: UniProtKB
  4. negative regulation of Wnt signaling pathway Source: UniProtKB
  5. protein localization Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Differentiation

Enzyme and pathway databases

ReactomeiREACT_264178. deactivation of the beta-catenin transactivating complex.
SignaLinkiQ9Y3M2.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein chibby homolog 1
Alternative name(s):
ARPP-binding protein
Cytosolic leucine-rich protein
PIGEA-14
PKD2 interactor, Golgi and endoplasmic reticulum-associated 1
Gene namesi
Name:CBY1
Synonyms:ARB1, C22orf2, CBY, PGEA1
ORF Names:HRIHFB2025
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:1307. CBY1.

Subcellular locationi

  1. Nucleus speckle
  2. Golgi apparatustrans-Golgi network

  3. Note: Nuclear, in a punctate manner. Also found in the trans-Golgi.

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. nuclear speck Source: UniProtKB-SubCell
  3. nucleoplasm Source: Reactome
  4. nucleus Source: UniProtKB
  5. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25886.

Polymorphism and mutation databases

BioMutaiCBY1.
DMDMi20454882.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 126126Protein chibby homolog 1PRO_0000058354Add
BLAST

Proteomic databases

MaxQBiQ9Y3M2.
PaxDbiQ9Y3M2.
PRIDEiQ9Y3M2.

PTM databases

PhosphoSiteiQ9Y3M2.

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors.1 Publication

Gene expression databases

BgeeiQ9Y3M2.
CleanExiHS_CBY1.
ExpressionAtlasiQ9Y3M2. baseline and differential.
GenevestigatoriQ9Y3M2.

Interactioni

Subunit structurei

Homodimer. Interacts with polycystin-2/PKD2 and GM130. Interacts with the C-terminal region of beta-catenin.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CFAP53Q96M913EBI-947308,EBI-742422
FAM92A1A1XBS53EBI-947308,EBI-2349888
TTC23LQ6PF05-33EBI-947308,EBI-10182647

Protein-protein interaction databases

BioGridi117311. 9 interactions.
DIPiDIP-29651N.
IntActiQ9Y3M2. 10 interactions.
MINTiMINT-2871551.
STRINGi9606.ENSP00000216029.

Structurei

3D structure databases

DisProtiDP00709.
ProteinModelPortaliQ9Y3M2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 11253Minimal region for the interaction with PKD2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili67 – 12559Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the chibby family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG39404.
GeneTreeiENSGT00510000047043.
HOGENOMiHOG000006694.
HOVERGENiHBG050816.
InParanoidiQ9Y3M2.
OMAiLEYGTPT.
PhylomeDBiQ9Y3M2.
TreeFamiTF324419.

Family and domain databases

InterProiIPR028118. Chibby_fam.
[Graphical view]
PfamiPF14645. Chibby. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y3M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFFGNTFSP KKTPPRKSAS LSNLHSLDRS TREVELGLEY GSPTMNLAGQ
60 70 80 90 100
SLKFENGQWI AETGVSGGVD RREVQRLRRR NQQLEEENNL LRLKVDILLD
110 120
MLSESTAESH LMEKELDELR ISRKRK
Length:126
Mass (Da):14,470
Last modified:November 1, 1999 - v1
Checksum:i0D243AD2CC436E55
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731E → K in AAL56062 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331041 mRNA. Translation: AAL56062.1.
AB111855 mRNA. Translation: BAC78839.1.
AL136686 mRNA. Translation: CAB66621.1.
AL050345 mRNA. Translation: CAB43547.1.
CR456410 mRNA. Translation: CAG30296.1.
AK311928 mRNA. Translation: BAG34869.1.
AL021707 Genomic DNA. Translation: CAB63442.1.
CH471095 Genomic DNA. Translation: EAW60254.1.
BC016139 mRNA. Translation: AAH16139.1.
AB015347 mRNA. Translation: BAA88119.1.
BK005534 Genomic DNA. Translation: DAA05582.1.
CCDSiCCDS13974.1.
RefSeqiNP_001002880.2. NM_001002880.1.
NP_056188.1. NM_015373.3.
UniGeneiHs.334911.
Hs.744261.

Genome annotation databases

EnsembliENST00000216029; ENSP00000216029; ENSG00000100211.
ENST00000396811; ENSP00000380026; ENSG00000100211.
GeneIDi25776.
KEGGihsa:25776.
UCSCiuc003awb.4. human.

Polymorphism and mutation databases

BioMutaiCBY1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331041 mRNA. Translation: AAL56062.1.
AB111855 mRNA. Translation: BAC78839.1.
AL136686 mRNA. Translation: CAB66621.1.
AL050345 mRNA. Translation: CAB43547.1.
CR456410 mRNA. Translation: CAG30296.1.
AK311928 mRNA. Translation: BAG34869.1.
AL021707 Genomic DNA. Translation: CAB63442.1.
CH471095 Genomic DNA. Translation: EAW60254.1.
BC016139 mRNA. Translation: AAH16139.1.
AB015347 mRNA. Translation: BAA88119.1.
BK005534 Genomic DNA. Translation: DAA05582.1.
CCDSiCCDS13974.1.
RefSeqiNP_001002880.2. NM_001002880.1.
NP_056188.1. NM_015373.3.
UniGeneiHs.334911.
Hs.744261.

3D structure databases

DisProtiDP00709.
ProteinModelPortaliQ9Y3M2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117311. 9 interactions.
DIPiDIP-29651N.
IntActiQ9Y3M2. 10 interactions.
MINTiMINT-2871551.
STRINGi9606.ENSP00000216029.

PTM databases

PhosphoSiteiQ9Y3M2.

Polymorphism and mutation databases

BioMutaiCBY1.
DMDMi20454882.

Proteomic databases

MaxQBiQ9Y3M2.
PaxDbiQ9Y3M2.
PRIDEiQ9Y3M2.

Protocols and materials databases

DNASUi25776.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216029; ENSP00000216029; ENSG00000100211.
ENST00000396811; ENSP00000380026; ENSG00000100211.
GeneIDi25776.
KEGGihsa:25776.
UCSCiuc003awb.4. human.

Organism-specific databases

CTDi25776.
GeneCardsiGC22P039052.
HGNCiHGNC:1307. CBY1.
MIMi607757. gene.
neXtProtiNX_Q9Y3M2.
PharmGKBiPA25886.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG39404.
GeneTreeiENSGT00510000047043.
HOGENOMiHOG000006694.
HOVERGENiHBG050816.
InParanoidiQ9Y3M2.
OMAiLEYGTPT.
PhylomeDBiQ9Y3M2.
TreeFamiTF324419.

Enzyme and pathway databases

ReactomeiREACT_264178. deactivation of the beta-catenin transactivating complex.
SignaLinkiQ9Y3M2.

Miscellaneous databases

ChiTaRSiCBY1. human.
GeneWikiiCBY1.
GenomeRNAii25776.
NextBioi46914.
PROiQ9Y3M2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y3M2.
CleanExiHS_CBY1.
ExpressionAtlasiQ9Y3M2. baseline and differential.
GenevestigatoriQ9Y3M2.

Family and domain databases

InterProiIPR028118. Chibby_fam.
[Graphical view]
PfamiPF14645. Chibby. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel cytosolic leucine-rich protein."
    Huang C.-H.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Human ARPP-binding protein, arb1."
    Moriyama M., Nakada C., Tsukamoto Y., Baba T., Kondo G., Ishiguro N., Horiuchi M., Sekine C., Maeda A.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Reevaluating human gene annotation: a second-generation analysis of chromosome 22."
    Collins J.E., Goward M.E., Cole C.G., Smink L.J., Huckle E.J., Knowles S., Bye J.M., Beare D.M., Dunham I.
    Genome Res. 13:27-36(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  7. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  10. "HRI NTT human fetal brain cDNA project."
    Ueki N.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-126.
    Tissue: Fetal brain.
  11. "Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway."
    Takemaru K., Yamaguchi S., Lee Y.S., Zhang Y., Carthew R.W., Moon R.T.
    Nature 422:905-909(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH BETA-CATENIN.
  12. "PIGEA-14, a novel coiled-coil protein affecting the intracellular distribution of polycystin-2."
    Hidaka S., Koenecke V., Osten L., Witzgall R.
    J. Biol. Chem. 279:35009-35016(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PKD2 AND GM130.
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCBY1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3M2
Secondary accession number(s): B2R4S2, Q66GT6, Q9UIK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1999
Last modified: April 29, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

'Chibby' is Japanese for 'small'; the gene was so named for the RNAi phenotype seen in flies.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.