Reviewed, UniProtKB/Swiss-Prot Q9Y3L3 (3BP1_HUMAN)

Names and origin

Protein names

Recommended name:
    SH3 domain-binding protein 1
      Short name=3BP-1

Gene names

Name:

SH3BP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac By similarity.
Sequence similarities	Contains 1 BAR domain. Contains 1 Rho-GAP domain.
Sequence caution	The sequence BAC85842.1 differs from that shown. Reason: Erroneous termination at position 56. Translated as Cys.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH3-binding
Molecular function	GTPase activation
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HCK	P08631	1	EBI-346869,EBI-346340

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y3L3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y3L3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     440-452: DQAQLDAASVSSI → TEPARELGSQTLC
     453-701: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	SH3 domain-binding protein 1		PRO_0000056723
Regions
	Domain	17 – 262	246	BAR
	Domain	276 – 469	194	Rho-GAP
	Motif	617 – 625	9	SH3-binding By similarity
Natural variations
	Alternative sequence	440 – 452	13	DQAQL…SVSSI → TEPARELGSQTLC in isoform 2.		VSP_011373
	Alternative sequence	453 – 701	249	Missing in isoform 2.		VSP_011374
	Natural variant	431	1	L → P: dbSNP rs929038.		VAR_019642
	Natural variant	511	1	P → L: dbSNP rs929038.		VAR_033450
	Natural variant	656	1	S → F: dbSNP rs2269548.		VAR_033451

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 16, 2004. Version 3. Checksum: 877D144E81F0F974	FASTA	701	75,713
	10 20 30 40 50 60 MMKRQLHRMR QLAQTGSLGR TPETAEFLGE DLLQVEQRLE PAKRAAHNIH KRLQACLQGQ 70 80 90 100 110 120 SGADMDKRVK KLPLMALSTT MAESFKELDP DSSMGKALEM SCAIQNQLAR ILAEFEMTLE 130 140 150 160 170 180 RDVLQPLSRL SEEELPAILK HKKSLQKLVS DWNTLKSRLS QATKNSGSSQ GLGGSPGSHS 190 200 210 220 230 240 HTTMANKVET LKEEEEELKR KVEQCRDEYL ADLYHFVTKE DSYANYFIRL LEIQADYHRR 250 260 270 280 290 300 SLSSLDTALA ELRENHGQAD HSPSMTATHF PRVYGVSLAT HLQELGREIA LPIEACVMML 310 320 330 340 350 360 LSEGMKEEGL FRLAAGASVL KRLKQTMASD PHSLEEFCSD PHAVAGALKS YLRELPEPLM 370 380 390 400 410 420 TFDLYDDWMR AASLKEPGAR LQALQEVCSR LPPENLSNLR YLMKFLARLA EEQEVNKMTP 430 440 450 460 470 480 SNIAIVLGPN LLWPPEKEGD QAQLDAASVS SIQVVGVVEA LIQSADTLFP GDINFNVSGL 490 500 510 520 530 540 FSAVTLQDTV SDRLASEELP STAVPTPATT PAPAPAPAPA PAPALASAAT KERTESEVPP 550 560 570 580 590 600 RPASPKVTRS PPETAAPVED MARRTKRPAP ARPTMPPPQV SGSRSSPPAP PLPPGSGSPG 610 620 630 640 650 660 TPQALPRRLV GSSLRAPTVP PPLPPTPPQP ARRQSRRSPA SPSPASPGPA SPSPVSLSNP 670 680 690 700 AQVDLGAATA EGGAPEAISG VPTPPAIPPQ PRPRSLASET N
	Isoform 2 [UniParc]. Checksum: A2C33CFBB2532FD0		452	50,820
	10 20 30 40 50 60 MMKRQLHRMR QLAQTGSLGR TPETAEFLGE DLLQVEQRLE PAKRAAHNIH KRLQACLQGQ 70 80 90 100 110 120 SGADMDKRVK KLPLMALSTT MAESFKELDP DSSMGKALEM SCAIQNQLAR ILAEFEMTLE 130 140 150 160 170 180 RDVLQPLSRL SEEELPAILK HKKSLQKLVS DWNTLKSRLS QATKNSGSSQ GLGGSPGSHS 190 200 210 220 230 240 HTTMANKVET LKEEEEELKR KVEQCRDEYL ADLYHFVTKE DSYANYFIRL LEIQADYHRR 250 260 270 280 290 300 SLSSLDTALA ELRENHGQAD HSPSMTATHF PRVYGVSLAT HLQELGREIA LPIEACVMML 310 320 330 340 350 360 LSEGMKEEGL FRLAAGASVL KRLKQTMASD PHSLEEFCSD PHAVAGALKS YLRELPEPLM 370 380 390 400 410 420 TFDLYDDWMR AASLKEPGAR LQALQEVCSR LPPENLSNLR YLMKFLARLA EEQEVNKMTP 430 440 450 SNIAIVLGPN LLWPPEKEGT EPARELGSQT LC

References

[1]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
[3]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-701 (ISOFORM 1). Tissue: Pancreas.
[5]	The German cDNA consortium Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 360-701 (ISOFORM 1). Tissue: Melanoma.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR456576 mRNA. Translation: CAG30462.1. AK124370 mRNA. Translation: BAC85842.1. Different initiation. Z83844 Genomic DNA. Translation: CAI20370.2. BC008282 mRNA. Translation: AAH08282.1. Different initiation. AL157480 mRNA. Translation: CAB75671.2.
PIR	T46916.
RefSeq	NP_061830.3.
UniGene	Hs.601143
3D structure databases
HSSP	HSSP built from PDB template 1F7C based on UniProtKB Q98935.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y3L3.
Genome annotation databases
Ensembl	ENSG00000100092. Homo sapiens. [Contig view]
GeneID	23616.
KEGG	hsa:23616.
Organism-specific databases
H-InvDB	HIX0016449.
HGNC	HGNC:10824. SH3BP1.
HPA	HPA000757.
PharmGKB	PA35732.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q9Y3L3.
HOVERGEN	Q9Y3L3.
Enzyme and pathway databases
Reactome	REACT_11044. Signaling by Rho GTPases.
Gene expression databases
ArrayExpress	Q9Y3L3.
CleanEx	HS_SH3BP1.
GermOnline	ENSG00000100092. Homo sapiens.
Family and domain databases
InterPro	IPR004148. BAR. IPR000198. RhoGAP. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF03114. BAR. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. [Graphical view]
PROSITE	PS51021. BAR. 1 hit. PS50238. RHOGAP. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
NextBio	46338.

Entry information

Entry name

3BP1_HUMAN

Accession

Primary (citable) accession number: Q9Y3L3
Secondary accession number(s): Q5R3N0 , Q6IBZ2, Q6ZVL9, Q96HQ5, Q9NSQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	August 16, 2004
Last modified:	November 4, 2008
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)