Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F-box only protein 7

Gene

FBXO7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5. Plays a role downstream of PINK1 in the clearance of damaged mitochondria via selective autophagy (mitophagy) by targeting PARK2 to dysfunctional depolarized mitochondria. Promotes MFN1 ubiquitination.3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • protein kinase binding Source: ParkinsonsUK-UCL
  • ubiquitin binding Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL
  • ubiquitin-protein transferase activity Source: ProtInc

GO - Biological processi

  • mitophagy Source: UniProtKB
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
  • negative regulation of G1/S transition of mitotic cell cycle Source: Ensembl
  • negative regulation of lymphocyte differentiation Source: Ensembl
  • negative regulation of oxidative stress-induced neuron death Source: ParkinsonsUK-UCL
  • positive regulation of mitophagy Source: ParkinsonsUK-UCL
  • protein targeting to mitochondrion Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: ParkinsonsUK-UCL
  • regulation of locomotion Source: ParkinsonsUK-UCL
  • regulation of neuron projection development Source: ParkinsonsUK-UCL
  • regulation of protein stability Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100225-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 7
Gene namesi
Name:FBXO7
Synonyms:FBX7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:13586. FBXO7.

Subcellular locationi

GO - Cellular componenti

  • classical Lewy body Source: ParkinsonsUK-UCL
  • cytoplasm Source: ParkinsonsUK-UCL
  • cytosol Source: UniProtKB
  • glial cytoplasmic inclusion Source: ParkinsonsUK-UCL
  • Lewy body core Source: ParkinsonsUK-UCL
  • Lewy body corona Source: ParkinsonsUK-UCL
  • Lewy neurite Source: ParkinsonsUK-UCL
  • mitochondrion Source: UniProtKB
  • nucleus Source: ParkinsonsUK-UCL
  • protein complex Source: LIFEdb
  • SCF ubiquitin ligase complex Source: ParkinsonsUK-UCL
  • ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Involvement in diseasei

Parkinson disease 15 (PARK15)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA neurodegenerative disorder characterized by parkinsonian and pyramidal signs. Clinical manifestations include tremor, bradykinesia, rigidity, postural instability, spasticity, mainly in the lower limbs, and hyperreflexia.
See also OMIM:260300
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047938378R → G in PARK15; no effect on interaction with PARK2. 2 PublicationsCorresponds to variant rs71799110dbSNPEnsembl.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi22T → M: Impairs interaction with PARK2. 1 Publication1
Mutagenesisi253V → E: Abolishes interaction with PSMF1. 1 Publication1

Keywords - Diseasei

Disease mutation, Neurodegeneration, Parkinsonism

Organism-specific databases

DisGeNETi25793.
MalaCardsiFBXO7.
MIMi260300. phenotype.
OpenTargetsiENSG00000100225.
Orphaneti171695. Parkinsonian-pyramidal syndrome.
PharmGKBiPA28047.

Polymorphism and mutation databases

BioMutaiFBXO7.
DMDMi13124249.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198851 – 522F-box only protein 7Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei432Omega-N-methylarginineBy similarity1
Modified residuei451Omega-N-methylarginineBy similarity1
Modified residuei518Asymmetric dimethylarginineBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

EPDiQ9Y3I1.
PaxDbiQ9Y3I1.
PeptideAtlasiQ9Y3I1.
PRIDEiQ9Y3I1.

PTM databases

iPTMnetiQ9Y3I1.
PhosphoSitePlusiQ9Y3I1.
SwissPalmiQ9Y3I1.

Expressioni

Gene expression databases

BgeeiENSG00000100225.
CleanExiHS_FBXO7.
ExpressionAtlasiQ9Y3I1. baseline and differential.
GenevisibleiQ9Y3I1. HS.

Organism-specific databases

HPAiCAB034296.
HPA032113.
HPA032114.

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO7) formed of CUL1, SKP1, RBX1 and FBXO7. Interacts via its C-terminal proline-rich region with DLGAP5. Interacts with BIRC2. Interacts with CDK6 and promotes its interaction with D-type cyclin. Isoform 1 interacts (via the N-terminal Ubl domain) with PARK2. Isoforms that lack the ubiquitin-like domain do not interact with PARK2. Isoform 1 and isoform 2 interact (via N-terminal region) with PINK1. Isoform 3 does not interact with PINK1. Interacts with PSMF1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PARK2O6026010EBI-1161222,EBI-716346
PINK1Q9BXM78EBI-1161222,EBI-2846068
PSMA3P257883EBI-1161222,EBI-348380
PSME3P612893EBI-1161222,EBI-355546
PSMF1Q925304EBI-1161222,EBI-945916
SKP1P632087EBI-1161222,EBI-307486

GO - Molecular functioni

  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • protein kinase binding Source: ParkinsonsUK-UCL
  • ubiquitin binding Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi117326. 71 interactors.
DIPiDIP-36125N.
IntActiQ9Y3I1. 36 interactors.
MINTiMINT-108554.
STRINGi9606.ENSP00000266087.

Structurei

Secondary structure

1522
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi181 – 190Combined sources10
Helixi195 – 209Combined sources15
Beta strandi213 – 216Combined sources4
Turni220 – 222Combined sources3
Beta strandi228 – 230Combined sources3
Beta strandi233 – 239Combined sources7
Helixi241 – 243Combined sources3
Beta strandi247 – 255Combined sources9
Beta strandi258 – 267Combined sources10
Beta strandi270 – 279Combined sources10
Helixi281 – 283Combined sources3
Helixi287 – 289Combined sources3
Helixi294 – 297Combined sources4
Helixi301 – 311Combined sources11
Helixi313 – 323Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4L9CX-ray2.10A/B180-335[»]
4L9HX-ray2.00A180-335[»]
ProteinModelPortaliQ9Y3I1.
SMRiQ9Y3I1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini329 – 375F-boxPROSITE-ProRule annotationAdd BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 88Ubiquitin-likeAdd BLAST88
Regioni92 – 129Important for interaction with PINK11 PublicationAdd BLAST38
Regioni129 – 169Important for interaction with CDK6Add BLAST41
Regioni180 – 324Important for dimerization and interaction with PSMF11 PublicationAdd BLAST145
Regioni381 – 522Important for interaction with CDK6Add BLAST142

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi481 – 484RFDP motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi423 – 514Pro-richAdd BLAST92

Domaini

The ubiquitin-like region mediates interaction with PARK2.
The proline-rich region is important for protein-protein interactions.

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGV9. Eukaryota.
ENOG410XQUZ. LUCA.
GeneTreeiENSGT00390000006670.
HOGENOMiHOG000112551.
HOVERGENiHBG005648.
InParanoidiQ9Y3I1.
KOiK10293.
OMAiPMLCSES.
OrthoDBiEOG091G0FNN.
PhylomeDBiQ9Y3I1.
TreeFamiTF329830.

Family and domain databases

InterProiIPR001810. F-box_dom.
IPR021625. PI31_Prot_N.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF11566. PI31_Prot_N. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y3I1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLRVRLLKR TWPLEVPETE PTLGHLRSHL RQSLLCTWGY SSNTRFTITL
60 70 80 90 100
NYKDPLTGDE ETLASYGIVS GDLICLILQD DIPAPNIPSS TDSEHSSLQN
110 120 130 140 150
NEQPSLATSS NQTSMQDEQP SDSFQGQAAQ SGVWNDDSML GPSQNFEAES
160 170 180 190 200
IQDNAHMAEG TGFYPSEPML CSESVEGQVP HSLETLYQSA DCSDANDALI
210 220 230 240 250
VLIHLLMLES GYIPQGTEAK ALSMPEKWKL SGVYKLQYMH PLCEGSSATL
260 270 280 290 300
TCVPLGNLIV VNATLKINNE IRSVKRLQLL PESFICKEKL GENVANIYKD
310 320 330 340 350
LQKLSRLFKD QLVYPLLAFT RQALNLPDVF GLVVLPLELK LRIFRLLDVR
360 370 380 390 400
SVLSLSAVCR DLFTASNDPL LWRFLYLRDF RDNTVRVQDT DWKELYRKRH
410 420 430 440 450
IQRKESPKGR FVMLLPSSTH TIPFYPNPLH PRPFPSSRLP PGIIGGEYDQ
460 470 480 490 500
RPTLPYVGDP ISSLIPGPGE TPSQFPPLRP RFDPVGPLPG PNPILPGRGG
510 520
PNDRFPFRPS RGRPTDGRLS FM
Length:522
Mass (Da):58,503
Last modified:November 1, 1999 - v1
Checksum:iC4E5E70A0747287A
GO
Isoform 2 (identifier: Q9Y3I1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     80-91: DDIPAPNIPSST → MARPPGGSGPLL

Show »
Length:443
Mass (Da):49,358
Checksum:i263A319CE0096FFB
GO
Isoform 3 (identifier: Q9Y3I1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Note: No experimental confirmation available.
Show »
Length:408
Mass (Da):45,781
Checksum:iFE4FE5ECFEAC37C5
GO

Sequence cautioni

The sequence AAF04471 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79Q → H in AAF04471 (PubMed:10531035).Curated1
Sequence conflicti84A → P in AAF04471 (PubMed:10531035).Curated1
Sequence conflicti154N → S in BAG63187 (PubMed:14702039).Curated1
Sequence conflicti169M → L in AAF04471 (PubMed:10531035).Curated1
Sequence conflicti224M → L in AAF04471 (PubMed:10531035).Curated1
Sequence conflicti241P → H in AAF04471 (PubMed:10531035).Curated1
Sequence conflicti328D → N in AAF04471 (PubMed:10531035).Curated1
Sequence conflicti413M → L in AAF04471 (PubMed:10531035).Curated1
Sequence conflicti475F → L in AAF04471 (PubMed:10531035).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021408115M → I.4 PublicationsCorresponds to variant rs11107dbSNPEnsembl.1
Natural variantiVAR_047938378R → G in PARK15; no effect on interaction with PARK2. 2 PublicationsCorresponds to variant rs71799110dbSNPEnsembl.1
Natural variantiVAR_066022481R → C Found in two patients with Kufor-Rakeb syndrome also carrying R-877 in ATP13A2. 1 PublicationCorresponds to variant rs148272407dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0447231 – 114Missing in isoform 3. 1 PublicationAdd BLAST114
Alternative sequenceiVSP_0410731 – 79Missing in isoform 2. 1 PublicationAdd BLAST79
Alternative sequenceiVSP_04107480 – 91DDIPA…IPSST → MARPPGGSGPLL in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233225 mRNA. Translation: AAF67155.1.
AL050254 mRNA. Translation: CAB43356.1.
CR456491 mRNA. Translation: CAG30377.1.
AK297841 mRNA. Translation: BAG60175.1.
AK301716 mRNA. Translation: BAG63187.1.
AL021937, AL035068, Z71183 Genomic DNA. Translation: CAI19587.1.
AL021937, AL035068, Z71183 Genomic DNA. Translation: CAI19588.2.
AL035068, AL021937, Z71183 Genomic DNA. Translation: CAI19333.1.
AL035068, AL021937, Z71183 Genomic DNA. Translation: CAI19334.2.
Z71183, AL021937, AL035068 Genomic DNA. Translation: CAI18782.1.
Z71183, AL021937, AL035068 Genomic DNA. Translation: CAI18783.2.
BC008361 mRNA. Translation: AAH08361.1.
AF129537 mRNA. Translation: AAF04471.1. Different initiation.
CCDSiCCDS13907.1. [Q9Y3I1-1]
CCDS46695.1. [Q9Y3I1-2]
CCDS58806.1. [Q9Y3I1-3]
RefSeqiNP_001028196.1. NM_001033024.1. [Q9Y3I1-2]
NP_001244919.1. NM_001257990.1. [Q9Y3I1-3]
NP_036311.3. NM_012179.3. [Q9Y3I1-1]
UniGeneiHs.5912.

Genome annotation databases

EnsembliENST00000266087; ENSP00000266087; ENSG00000100225. [Q9Y3I1-1]
ENST00000397426; ENSP00000380571; ENSG00000100225. [Q9Y3I1-3]
ENST00000452138; ENSP00000388547; ENSG00000100225. [Q9Y3I1-2]
GeneIDi25793.
KEGGihsa:25793.
UCSCiuc003amq.4. human. [Q9Y3I1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233225 mRNA. Translation: AAF67155.1.
AL050254 mRNA. Translation: CAB43356.1.
CR456491 mRNA. Translation: CAG30377.1.
AK297841 mRNA. Translation: BAG60175.1.
AK301716 mRNA. Translation: BAG63187.1.
AL021937, AL035068, Z71183 Genomic DNA. Translation: CAI19587.1.
AL021937, AL035068, Z71183 Genomic DNA. Translation: CAI19588.2.
AL035068, AL021937, Z71183 Genomic DNA. Translation: CAI19333.1.
AL035068, AL021937, Z71183 Genomic DNA. Translation: CAI19334.2.
Z71183, AL021937, AL035068 Genomic DNA. Translation: CAI18782.1.
Z71183, AL021937, AL035068 Genomic DNA. Translation: CAI18783.2.
BC008361 mRNA. Translation: AAH08361.1.
AF129537 mRNA. Translation: AAF04471.1. Different initiation.
CCDSiCCDS13907.1. [Q9Y3I1-1]
CCDS46695.1. [Q9Y3I1-2]
CCDS58806.1. [Q9Y3I1-3]
RefSeqiNP_001028196.1. NM_001033024.1. [Q9Y3I1-2]
NP_001244919.1. NM_001257990.1. [Q9Y3I1-3]
NP_036311.3. NM_012179.3. [Q9Y3I1-1]
UniGeneiHs.5912.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4L9CX-ray2.10A/B180-335[»]
4L9HX-ray2.00A180-335[»]
ProteinModelPortaliQ9Y3I1.
SMRiQ9Y3I1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117326. 71 interactors.
DIPiDIP-36125N.
IntActiQ9Y3I1. 36 interactors.
MINTiMINT-108554.
STRINGi9606.ENSP00000266087.

PTM databases

iPTMnetiQ9Y3I1.
PhosphoSitePlusiQ9Y3I1.
SwissPalmiQ9Y3I1.

Polymorphism and mutation databases

BioMutaiFBXO7.
DMDMi13124249.

Proteomic databases

EPDiQ9Y3I1.
PaxDbiQ9Y3I1.
PeptideAtlasiQ9Y3I1.
PRIDEiQ9Y3I1.

Protocols and materials databases

DNASUi25793.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000266087; ENSP00000266087; ENSG00000100225. [Q9Y3I1-1]
ENST00000397426; ENSP00000380571; ENSG00000100225. [Q9Y3I1-3]
ENST00000452138; ENSP00000388547; ENSG00000100225. [Q9Y3I1-2]
GeneIDi25793.
KEGGihsa:25793.
UCSCiuc003amq.4. human. [Q9Y3I1-1]

Organism-specific databases

CTDi25793.
DisGeNETi25793.
GeneCardsiFBXO7.
GeneReviewsiFBXO7.
HGNCiHGNC:13586. FBXO7.
HPAiCAB034296.
HPA032113.
HPA032114.
MalaCardsiFBXO7.
MIMi260300. phenotype.
605648. gene.
neXtProtiNX_Q9Y3I1.
OpenTargetsiENSG00000100225.
Orphaneti171695. Parkinsonian-pyramidal syndrome.
PharmGKBiPA28047.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGV9. Eukaryota.
ENOG410XQUZ. LUCA.
GeneTreeiENSGT00390000006670.
HOGENOMiHOG000112551.
HOVERGENiHBG005648.
InParanoidiQ9Y3I1.
KOiK10293.
OMAiPMLCSES.
OrthoDBiEOG091G0FNN.
PhylomeDBiQ9Y3I1.
TreeFamiTF329830.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000100225-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiFBXO7. human.
GeneWikiiFBXO7.
GenomeRNAii25793.
PROiQ9Y3I1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100225.
CleanExiHS_FBXO7.
ExpressionAtlasiQ9Y3I1. baseline and differential.
GenevisibleiQ9Y3I1. HS.

Family and domain databases

InterProiIPR001810. F-box_dom.
IPR021625. PI31_Prot_N.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF11566. PI31_Prot_N. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBX7_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3I1
Secondary accession number(s): B4DNB3
, B4DWX5, Q5TGC4, Q5TI86, Q96HM6, Q9UF21, Q9UKT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.