true
2006-10-31
2024-03-27
184
RTCB_HUMAN
Reevaluating human gene annotation: a second-generation analysis of chromosome 22.
Collins J.E.
Goward M.E.
Cole C.G.
Smink L.J.
Huckle E.J.
Knowles S.
Bye J.M.
Beare D.M.
Dunham I.
doi:10.1101/gr.695703
2003
Genome Res.
13
27-36
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.
Zhang Q.-H.
Ye M.
Wu X.-Y.
Ren S.-X.
Zhao M.
Zhao C.-J.
Fu G.
Shen Y.
Fan H.-Y.
Lu G.
Zhong M.
Xu X.-R.
Han Z.-G.
Zhang J.-W.
Tao J.
Huang Q.-H.
Zhou J.
Hu G.-X.
Gu J.
Chen S.-J.
Chen Z.
doi:10.1101/gr.140200
2000
Genome Res.
10
1546-1560
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
Umbilical cord blood
Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.
Hu R.-M.
Han Z.-G.
Song H.-D.
Peng Y.-D.
Huang Q.-H.
Ren S.-X.
Gu Y.-J.
Huang C.-H.
Li Y.-B.
Jiang C.-L.
Fu G.
Zhang Q.-H.
Gu B.-W.
Dai M.
Mao Y.-F.
Gao G.-F.
Rong R.
Ye M.
Zhou J.
Xu S.-H.
Gu J.
Shi J.-X.
Jin W.-R.
Zhang C.-K.
Wu T.-M.
Huang G.-Y.
Chen Z.
Chen M.-D.
Chen J.-L.
doi:10.1073/pnas.160270997
2000
Proc. Natl. Acad. Sci. U.S.A.
97
9543-9548
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
Adrenal gland
Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).
Ebert L.
Schick M.
Neubert P.
Schatten R.
Henze S.
Korn B.
2004-06
EMBL/GenBank/DDBJ
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
A genome annotation-driven approach to cloning the human ORFeome.
Collins J.E.
Wright C.L.
Edwards C.A.
Davis M.P.
Grinham J.A.
Cole C.G.
Goward M.E.
Aguado B.
Mallya M.
Mokrab Y.
Huckle E.J.
Beare D.M.
Dunham I.
doi:10.1186/gb-2004-5-10-r84
2004
Genome Biol.
5
R84.1-R84.11
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
Complete sequencing and characterization of 21,243 full-length human cDNAs.
Ota T.
Suzuki Y.
Nishikawa T.
Otsuki T.
Sugiyama T.
Irie R.
Wakamatsu A.
Hayashi K.
Sato H.
Nagai K.
Kimura K.
Makita H.
Sekine M.
Obayashi M.
Nishi T.
Shibahara T.
Tanaka T.
Ishii S.
Yamamoto J.
Saito K.
Kawai Y.
Isono Y.
Nakamura Y.
Nagahari K.
Murakami K.
Yasuda T.
Iwayanagi T.
Wagatsuma M.
Shiratori A.
Sudo H.
Hosoiri T.
Kaku Y.
Kodaira H.
Kondo H.
Sugawara M.
Takahashi M.
Kanda K.
Yokoi T.
Furuya T.
Kikkawa E.
Omura Y.
Abe K.
Kamihara K.
Katsuta N.
Sato K.
Tanikawa M.
Yamazaki M.
Ninomiya K.
Ishibashi T.
Yamashita H.
Murakawa K.
Fujimori K.
Tanai H.
Kimata M.
Watanabe M.
Hiraoka S.
Chiba Y.
Ishida S.
Ono Y.
Takiguchi S.
Watanabe S.
Yosida M.
Hotuta T.
Kusano J.
Kanehori K.
Takahashi-Fujii A.
Hara H.
Tanase T.-O.
Nomura Y.
Togiya S.
Komai F.
Hara R.
Takeuchi K.
Arita M.
Imose N.
Musashino K.
Yuuki H.
Oshima A.
Sasaki N.
Aotsuka S.
Yoshikawa Y.
Matsunawa H.
Ichihara T.
Shiohata N.
Sano S.
Moriya S.
Momiyama H.
Satoh N.
Takami S.
Terashima Y.
Suzuki O.
Nakagawa S.
Senoh A.
Mizoguchi H.
Goto Y.
Shimizu F.
Wakebe H.
Hishigaki H.
Watanabe T.
Sugiyama A.
Takemoto M.
Kawakami B.
Yamazaki M.'
Watanabe K.
Kumagai A.
Itakura S.
Fukuzumi Y.
Fujimori Y.
Komiyama M.
Tashiro H.
Tanigami A.
Fujiwara T.
Ono T.
Yamada K.
Fujii Y.
Ozaki K.
Hirao M.
Ohmori Y.
Kawabata A.
Hikiji T.
Kobatake N.
Inagaki H.
Ikema Y.
Okamoto S.
Okitani R.
Kawakami T.
Noguchi S.
Itoh T.
Shigeta K.
Senba T.
Matsumura K.
Nakajima Y.
Mizuno T.
Morinaga M.
Sasaki M.
Togashi T.
Oyama M.
Hata H.
Watanabe M.'
Komatsu T.
Mizushima-Sugano J.
Satoh T.
Shirai Y.
Takahashi Y.
Nakagawa K.
Okumura K.
Nagase T.
Nomura N.
Kikuchi H.
Masuho Y.
Yamashita R.
Nakai K.
Yada T.
Nakamura Y.'
Ohara O.
Isogai T.
Sugano S.
doi:10.1038/ng1285
2004
Nat. Genet.
36
40-45
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
Thalamus
The DNA sequence of human chromosome 22.
Dunham I.
Hunt A.R.
Collins J.E.
Bruskiewich R.
Beare D.M.
Clamp M.
Smink L.J.
Ainscough R.
Almeida J.P.
Babbage A.K.
Bagguley C.
Bailey J.
Barlow K.F.
Bates K.N.
Beasley O.P.
Bird C.P.
Blakey S.E.
Bridgeman A.M.
Buck D.
Burgess J.
Burrill W.D.
Burton J.
Carder C.
Carter N.P.
Chen Y.
Clark G.
Clegg S.M.
Cobley V.E.
Cole C.G.
Collier R.E.
Connor R.
Conroy D.
Corby N.R.
Coville G.J.
Cox A.V.
Davis J.
Dawson E.
Dhami P.D.
Dockree C.
Dodsworth S.J.
Durbin R.M.
Ellington A.G.
Evans K.L.
Fey J.M.
Fleming K.
French L.
Garner A.A.
Gilbert J.G.R.
Goward M.E.
Grafham D.V.
Griffiths M.N.D.
Hall C.
Hall R.E.
Hall-Tamlyn G.
Heathcott R.W.
Ho S.
Holmes S.
Hunt S.E.
Jones M.C.
Kershaw J.
Kimberley A.M.
King A.
Laird G.K.
Langford C.F.
Leversha M.A.
Lloyd C.
Lloyd D.M.
Martyn I.D.
Mashreghi-Mohammadi M.
Matthews L.H.
Mccann O.T.
Mcclay J.
Mclaren S.
McMurray A.A.
Milne S.A.
Mortimore B.J.
Odell C.N.
Pavitt R.
Pearce A.V.
Pearson D.
Phillimore B.J.C.T.
Phillips S.H.
Plumb R.W.
Ramsay H.
Ramsey Y.
Rogers L.
Ross M.T.
Scott C.E.
Sehra H.K.
Skuce C.D.
Smalley S.
Smith M.L.
Soderlund C.
Spragon L.
Steward C.A.
Sulston J.E.
Swann R.M.
Vaudin M.
Wall M.
Wallis J.M.
Whiteley M.N.
Willey D.L.
Williams L.
Williams S.A.
Williamson H.
Wilmer T.E.
Wilming L.
Wright C.L.
Hubbard T.
Bentley D.R.
Beck S.
Rogers J.
Shimizu N.
Minoshima S.
Kawasaki K.
Sasaki T.
Asakawa S.
Kudoh J.
Shintani A.
Shibuya K.
Yoshizaki Y.
Aoki N.
Mitsuyama S.
Roe B.A.
Chen F.
Chu L.
Crabtree J.
Deschamps S.
Do A.
Do T.
Dorman A.
Fang F.
Fu Y.
Hu P.
Hua A.
Kenton S.
Lai H.
Lao H.I.
Lewis J.
Lewis S.
Lin S.-P.
Loh P.
Malaj E.
Nguyen T.
Pan H.
Phan S.
Qi S.
Qian Y.
Ray L.
Ren Q.
Shaull S.
Sloan D.
Song L.
Wang Q.
Wang Y.
Wang Z.
White J.
Willingham D.
Wu H.
Yao Z.
Zhan M.
Zhang G.
Chissoe S.
Murray J.
Miller N.
Minx P.
Fulton R.
Johnson D.
Bemis G.
Bentley D.
Bradshaw H.
Bourne S.
Cordes M.
Du Z.
Fulton L.
Goela D.
Graves T.
Hawkins J.
Hinds K.
Kemp K.
Latreille P.
Layman D.
Ozersky P.
Rohlfing T.
Scheet P.
Walker C.
Wamsley A.
Wohldmann P.
Pepin K.
Nelson J.
Korf I.
Bedell J.A.
Hillier L.W.
Mardis E.
Waterston R.
Wilson R.
Emanuel B.S.
Shaikh T.
Kurahashi H.
Saitta S.
Budarf M.L.
McDermid H.E.
Johnson A.
Wong A.C.C.
Morrow B.E.
Edelmann L.
Kim U.J.
Shizuya H.
Simon M.I.
Dumanski J.P.
Peyrard M.
Kedra D.
Seroussi E.
Fransson I.
Tapia I.
Bruder C.E.
O'Brien K.P.
Wilkinson P.
Bodenteich A.
Hartman K.
Hu X.
Khan A.S.
Lane L.
Tilahun Y.
Wright H.
doi:10.1038/990031
1999
Nature
402
489-495
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
Mural R.J.
Istrail S.
Sutton G.G.
Florea L.
Halpern A.L.
Mobarry C.M.
Lippert R.
Walenz B.
Shatkay H.
Dew I.
Miller J.R.
Flanigan M.J.
Edwards N.J.
Bolanos R.
Fasulo D.
Halldorsson B.V.
Hannenhalli S.
Turner R.
Yooseph S.
Lu F.
Nusskern D.R.
Shue B.C.
Zheng X.H.
Zhong F.
Delcher A.L.
Huson D.H.
Kravitz S.A.
Mouchard L.
Reinert K.
Remington K.A.
Clark A.G.
Waterman M.S.
Eichler E.E.
Adams M.D.
Hunkapiller M.W.
Myers E.W.
Venter J.C.
2005-07
EMBL/GenBank/DDBJ
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
The MGC Project Team
doi:10.1101/gr.2596504
2004
Genome Res.
14
2121-2127
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
VARIANT PHE-343
Choriocarcinoma
Lung carcinoma
Bienvenut W.V.
Boldt K.
von Kriegsheim A.F.
Kolch W.
2007-07
UniProtKB
PROTEIN SEQUENCE OF 4-14; 45-51; 56-81; 141-158; 199-206; 264-279; 298-308; 442-460 AND 466-476
IDENTIFICATION BY MASS SPECTROMETRY
Hepatoma
The full-ORF clone resource of the German cDNA consortium.
Bechtel S.
Rosenfelder H.
Duda A.
Schmidt C.P.
Ernst U.
Wellenreuther R.
Mehrle A.
Schuster C.
Bahr A.
Bloecker H.
Heubner D.
Hoerlein A.
Michel G.
Wedler H.
Koehrer K.
Ottenwaelder B.
Poustka A.
Wiemann S.
Schupp I.
doi:10.1186/1471-2164-8-399
2007
BMC Genomics
8
399
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 309-505
Testis
Proteomic characterization of the human centrosome by protein correlation profiling.
Andersen J.S.
Wilkinson C.J.
Mayor T.
Mortensen P.
Nigg E.A.
Mann M.
doi:10.1038/nature02166
2003
Nature
426
570-574
IDENTIFICATION BY MASS SPECTROMETRY
Lymphoblast
Kinesin transports RNA: isolation and characterization of an RNA-transporting granule.
Kanai Y.
Dohmae N.
Hirokawa N.
doi:10.1016/j.neuron.2004.07.022
2004
Neuron
43
513-525
IDENTIFICATION IN A RNA TRANSPORT COMPLEX
Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.
Guo D.
Han J.
Adam B.-L.
Colburn N.H.
Wang M.-H.
Dong Z.
Eizirik D.L.
She J.-X.
Wang C.-Y.
doi:10.1016/j.bbrc.2005.09.191
2005
Biochem. Biophys. Res. Commun.
337
1308-1318
IDENTIFICATION BY MASS SPECTROMETRY
Initial characterization of the human central proteome.
Burkard T.R.
Planyavsky M.
Kaupe I.
Breitwieser F.P.
Buerckstuemmer T.
Bennett K.L.
Superti-Furga G.
Colinge J.
doi:10.1186/1752-0509-5-17
2011
BMC Syst. Biol.
5
17
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
HSPC117 is the essential subunit of a human tRNA splicing ligase complex.
Popow J.
Englert M.
Weitzer S.
Schleiffer A.
Mierzwa B.
Mechtler K.
Trowitzsch S.
Will C.L.
Luhrmann R.
Soll D.
Martinez J.
doi:10.1126/science.1197847
2011
Science
331
760-764
FUNCTION
CATALYTIC ACTIVITY
IDENTIFICATION BY MASS SPECTROMETRY
IDENTIFICATION IN THE TRNA SPLICING LIGASE COMPLEX
MUTAGENESIS OF CYS-122
Toward a comprehensive characterization of a human cancer cell phosphoproteome.
Zhou H.
Di Palma S.
Preisinger C.
Peng M.
Polat A.N.
Heck A.J.
Mohammed S.
doi:10.1021/pr300630k
2013
J. Proteome Res.
12
260-271
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
Cervix carcinoma
Erythroleukemia
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
Bian Y.
Song C.
Cheng K.
Dong M.
Wang F.
Huang J.
Sun D.
Wang L.
Ye M.
Zou H.
doi:10.1016/j.jprot.2013.11.014
2014
J. Proteomics
96
253-262
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
Liver
Analysis of orthologous groups reveals archease and DDX1 as tRNA splicing factors.
Popow J.
Jurkin J.
Schleiffer A.
Martinez J.
doi:10.1038/nature13284
2014
Nature
511
104-107
FUNCTION
CATALYTIC ACTIVITY
REACTION MECHANISM
ACTIVITY REGULATION
IDENTIFICATION IN THE TRNA SPLICING LIGASE COMPLEX
hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex.
Perez-Gonzalez A.
Pazo A.
Navajas R.
Ciordia S.
Rodriguez-Frandsen A.
Nieto A.
doi:10.1371/journal.pone.0090957
2014
PLoS ONE
9
E90957
SUBCELLULAR LOCATION
N-terminome analysis of the human mitochondrial proteome.
Vaca Jacome A.S.
Rabilloud T.
Schaeffer-Reiss C.
Rompais M.
Ayoub D.
Lane L.
Bairoch A.
Van Dorsselaer A.
Carapito C.
doi:10.1002/pmic.201400617
2015
Proteomics
15
2519-2524
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.
Hendriks I.A.
Lyon D.
Young C.
Jensen L.J.
Vertegaal A.C.
Nielsen M.L.
doi:10.1038/nsmb.3366
2017
Nat. Struct. Mol. Biol.
24
325-336
SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-496
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
Frameshift.
Frameshift.
2.30
A/B=2-505
358
tRNA-splicing ligase complex
85
Sucrose
1 site, 1 O-linked glycan (1 site)
267 antibodies from 29 providers
human
RTCB
Low tissue specificity
gene
Eukaryota
2681
tRNA processing in the nucleus
641 hits in 1140 CRISPR screens
human
Tbio
Protein
Expressed in jejunal mucosa and 216 other cell types or tissues
tRNA-splicing ligase RtcB
RtcB_euk
RtcB
RtcB-like_sf
RtcB_euk
RNA-SPLICING LIGASE RTCB HOMOLOG
UNCHARACTERIZED
RtcB
Hypothetical protein PH1602
UPF0027
HS
RNA-splicing ligase RtcB homolog
6.5.1.8
3'-phosphate/5'-hydroxy nucleic acid ligase
RTCB
C22orf28
HSPC117
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Binds 2 manganese ions per subunit.
Protein archease stimulates the activity of the tRNA ligase complex with high efficiency in the presence of GTP.
Catalytic component of the tRNA-splicing ligase complex.
Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low.
Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.
Belongs to the RtcB family.
RNA-splicing ligase RtcB homolog
55210
1
505
GMP-histidine intermediate
428
119
122
226
230
227
259
353
354
402
405
409
431
504
Phosphoserine
300
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
496
A
153
F
343
Abolishes tRNA ligase activity.
A
P
296
V
303
V
R
427
5
9
12
16
19
22
34
37
40
54
65
73
82
89
92
94
96
103
113
115
123
132
133
136
137
139
140
150
165
174
176
181
187
191
194
197
204
206
209
215
216
218
228
238
240
245
253
260
263
283
291
293
301
335
340
344
347
363
366
376
390
392
393
395
398
414
416
422
423
426
436
439
445
454
458
462
464
470
472
474
478
487
490
1
2
false
3
false
4
false
2
APPL1
RTRAF
ZBTB8OS
1999-11-01
1
55210
99736fcbe3fed94ce485fed1e47d0d54
MSRSYNDELQFLEKINKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIGNVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSARAKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG
true
true
true
true
true
true
true
true
true
true
true
true
true
true
true
true
true