Q9Y3D8 (KAD6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate kinase isoenzyme 6
EC=2.7.4.3

Alternative name(s):
ATP-AMP transphosphorylase 6
Coilin-interacting nuclear ATPase protein
Short name=hCINAP

Gene names

Name:	TAF9
Synonyms:	AK6, CINAP
ORF Names:	AD-004, CGI-137

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Broad activity as an NMP kinase. AMP and dAMP are the preferred substrates of all tested NMPs, but CMP and dCMP are also good substrates. IMP can be phosphorylated, but to a much lesser extent. Adenylate and cytidylate can serve as phosphate acceptors. Ref.8
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Interacts with COIL (via C-terminus). Ref.1
Subcellular location	Nucleus › nucleoplasm. Nucleus › Cajal body. Note: Displays widespread diffuse nucleoplasmic distribution but not detected in nucleoli. Detected in Cajal bodies but not in all cells. Ref.1 Ref.8
Sequence similarities	Belongs to the adenylate kinase family. AK6 subfamily.
Biophysicochemical properties	Kinetic parameters: KM=75.3 µM for ATP Ref.1 Vmax=1.27 µmol/min/mg enzyme

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Cajal body Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from direct assay Ref.8. Source: UniProtKB protein binding Inferred from physical interaction Ref.1. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COIL	P38432	5	EBI-2896123,EBI-945751

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoform 1 and isoform 2 are produced by the same gene but are translated from different initiation codons and use different reading frames so they display no identity in their protein sequences. This arrangement is conserved in mammals but these proteins are produced by separate genes in non-mammalian species.

Isoform 1 (identifier: Q9Y3D8-1)

Also known as: AK6;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q16594-1)

Also known as: TAF9;

The sequence of this isoform can be found in the external entry Q16594.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 3 (identifier: Q9Y3D8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-9: LLPNILLT → CHRKP

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 172

172

Adenylate kinase isoenzyme 6

PRO_0000153896

Regions

Nucleotide binding

10 – 17

8

ATP Potential

Natural variations

Alternative sequence

2 – 9

8

LLPNILLT → CHRKP in isoform 3.

VSP_039714

Secondary structure

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172

Helix Strand Turn

Details...

Beta strand

6 – 9

4

Helix

16 – 27

12

Beta strand

30 – 33

4

Helix

34 – 40

7

Beta strand

49 – 52

4

Helix

58 – 71

14

Beta strand

74 – 77

4

Helix

86 – 88

3

Beta strand

90 – 96

7

Helix

99 – 108

10

Helix

113 – 124

12

Helix

127 – 135

9

Helix

138 – 140

3

Beta strand

141 – 145

5

Helix

149 – 169

21

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (AK6) [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 33F62318FB434301	FASTA	172	20,061
	10 20 30 40 50 60 MLLPNILLTG TPGVGKTTLG KELASKSGLK YINVGDLARE EQLYDGYDEE YDCPILDEDR 70 80 90 100 110 120 VVDELDNQMR EGGVIVDYHG CDFFPERWFH IVFVLRTDTN VLYERLETRG YNEKKLTDNI 130 140 150 160 170 QCEIFQVLYE EATASYKEEI VHQLPSNKPE ELENNVDQIL KWIEQWIKDH NS
	Isoform 2 (TAF9) [UniParc]. See Q16594.		–	–
	Isoform 3 [UniParc]. Checksum: 3F341AE6C5898C66	FASTA	169	19,805
	10 20 30 40 50 60 MCHRKPGTPG VGKTTLGKEL ASKSGLKYIN VGDLAREEQL YDGYDEEYDC PILDEDRVVD 70 80 90 100 110 120 ELDNQMREGG VIVDYHGCDF FPERWFHIVF VLRTDTNVLY ERLETRGYNE KKLTDNIQCE 130 140 150 160 IFQVLYEEAT ASYKEEIVHQ LPSNKPEELE NNVDQILKWI EQWIKDHNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of hCINAP, a novel coilin-interacting protein encoded by a transcript from the transcription factor TAFIID32 locus." Santama N., Ogg S.C., Malekkou A., Zographos S.E., Weis K., Lamond A.I. J. Biol. Chem. 280:36429-36441(2005) [PubMed: 16079131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH COIL, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING. Tissue: Cervix carcinoma and Placenta.
[2]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland.
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"The crystal structure of human adenylate kinase 6: an adenylate kinase localized to the cell nucleus." Ren H., Wang L., Bennett M., Liang Y., Zheng X., Lu F., Li L., Nan J., Luo M., Eriksson S., Zhang C., Su X.-D. Proc. Natl. Acad. Sci. U.S.A. 102:303-308(2005) [PubMed: 15630091] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ878880 mRNA. Translation: CAI48030.1.
AJ878881 mRNA. Translation: CAI48031.1.
AF151895 mRNA. Translation: AAD34132.1.
AF110777 mRNA. Translation: AAF14860.1.
AC145132 Genomic DNA. No translation available.
CH471137 Genomic DNA. Translation: EAW51290.1.
CH471137 Genomic DNA. Translation: EAW51291.1.
CH471137 Genomic DNA. Translation: EAW51292.1.
CH471137 Genomic DNA. Translation: EAW51297.1.
BC007349 mRNA. Translation: AAH07349.1.
BC007426 mRNA. Translation: AAH07426.1.

IPI

IPI00032879.
IPI00556532.

RefSeq

NP_001015891.1. NM_001015891.1.
NP_057367.1. NM_016283.4.

UniGene

Hs.653163.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RKB	X-ray	2.00	A	1-172	[»]
3IIJ	X-ray	1.76	A	1-172	[»]
3IIK	X-ray	1.95	A	1-172	[»]
3IIL	X-ray	2.00	A	1-172	[»]
3IIM	X-ray	2.00	A	1-172	[»]

ProteinModelPortal

Q9Y3D8.

SMR

Q9Y3D8. Positions 1-172.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y3D8. 3 interactions.

MINT

MINT-1389230.

STRING

Q9Y3D8.

Polymorphism databases

DMDM

6831735.

Proteomic databases

PeptideAtlas

Q9Y3D8.

PRIDE

Q9Y3D8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000380818; ENSP00000370197; ENSG00000085231.
ENST00000380822; ENSP00000370201; ENSG00000085231.

GeneID

6880.

KEGG

hsa:6880.

UCSC

uc003jwa.1. human.

Organism-specific databases

CTD

6880.

GeneCards

GC05M068683.

HGNC

HGNC:11542. TAF9.

HPA

CAB005361.

neXtProt

NX_Q9Y3D8.

PharmGKB

PA36317.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00390000001626.

HOGENOM

HBG646389.

HOVERGEN

HBG052207.

InParanoid

Q9Y3D8.

OMA

GQLYDGY.

OrthoDB

EOG4X3H2N.

PhylomeDB

Q9Y3D8.

Gene expression databases

ArrayExpress

Q9Y3D8.

Bgee

Q9Y3D8.

Genevestigator

Q9Y3D8.

GermOnline

ENSG00000185057. Homo sapiens.

Family and domain databases

InterPro

IPR003959. ATPase_AAA_core.
[Graphical view]

KO

K14535.

Pfam

PF00004. AAA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

26873.

Entry information

Entry name

KAD6_HUMAN

Accession

Primary (citable) accession number: Q9Y3D8
Secondary accession number(s): A8MSZ6, Q5F2S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.