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Q9Y3D8

- KAD6_HUMAN

UniProt

Q9Y3D8 - KAD6_HUMAN

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Protein
Adenylate kinase isoenzyme 6
Gene
AK6, CINAP, AD-004, CGI-137
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. AMP and dAMP are the preferred substrates, but CMP and dCMP are also good substrates. IMP is phosphorylated to a much lesser extent. All nucleoside triphosphates ATP, GTP, UTP, CTP, dATP, dCTP, dGTP, and TTP are accepted as phosphate donors. CTP is the best phosphate donor, followed by UTP, ATP, GTP and dCTP. May have a role in nuclear energy homeostasis. Has also ATPase activity. May be involved in regulation of Cajal body (CB) formation.1 Publication

Catalytic activityi

ATP + AMP = 2 ADP.1 Publication

Kineticsi

kcat is 6.3 sec(-1) for adenylate kinase activity. kcat is 4.8 sec(-1) for ATPase activity.

  1. KM=192 µM for AMP3 Publications
  2. KM=45 µM for ATP (for adenylate kinase activity)
  3. KM=332 µM for ATP (for ATPase activity)

Vmax=1.27 µmol/min/mg enzyme for ATPase activity

Vmax=982 nmol/min/mg enzyme for the forward adenylate kinase reaction

Vmax=955 nmol/min/mg enzyme for the reverse adenylate kinase reaction

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391AMP Inferred
Binding sitei79 – 791ATP
Binding sitei105 – 1051ATP
Binding sitei109 – 1091ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186ATPUniRule annotation
Nucleotide bindingi148 – 1492ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity Source: UniProtKB-HAMAP
  3. adenylate kinase activity Source: UniProtKB
  4. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. nucleotide phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 6 (EC:2.7.4.3)
Short name:
AK6
Alternative name(s):
Adrenal gland protein AD-004
Coilin-interacting nuclear ATPase protein
Short name:
hCINAP
Dual activity adenylate kinase/ATPase
Short name:
AK/ATPase
Gene namesi
Name:AK6
Synonyms:CINAP
ORF Names:AD-004, CGI-137
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:49151. AK6.

Subcellular locationi

Nucleusnucleoplasm. NucleusCajal body
Note: Displays widespread diffuse nucleoplasmic distribution but not detected in nucleoli. Detected in Cajal bodies but not in all cells.2 Publications

GO - Cellular componenti

  1. Cajal body Source: UniProtKB
  2. nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi79 – 791H → G: Induces homodimerization. Reduces adenylate kinase activity by 72% and ATPase activity by 76%. Significantly changes Cajal body organization in the nucleus, resulting in enhanced apoptosis and reduced proliferation. 1 Publication

Organism-specific databases

PharmGKBiPA36317.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172Adenylate kinase isoenzyme 6UniRule annotation
PRO_0000153896Add
BLAST

Proteomic databases

MaxQBiQ9Y3D8.
PaxDbiQ9Y3D8.
PeptideAtlasiQ9Y3D8.
PRIDEiQ9Y3D8.

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, chorionic villi and the central nervous system.1 Publication

Gene expression databases

ArrayExpressiQ9Y3D8.
BgeeiQ9Y3D8.
GenevestigatoriQ9Y3D8.

Organism-specific databases

HPAiCAB005361.

Interactioni

Subunit structurei

Monomer and homodimer By similarity. Interacts with COIL (via C-terminus).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COILP384325EBI-2896123,EBI-945751

Protein-protein interaction databases

BioGridi112743. 70 interactions.
IntActiQ9Y3D8. 4 interactions.
MINTiMINT-1389230.
STRINGi9606.ENSP00000370201.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 94
Helixi16 – 2712
Beta strandi30 – 334
Helixi34 – 418
Beta strandi44 – 485
Turni49 – 524
Beta strandi53 – 564
Helixi58 – 7114
Beta strandi74 – 774
Helixi86 – 883
Beta strandi90 – 967
Helixi99 – 10810
Helixi113 – 12412
Helixi127 – 1359
Helixi138 – 1403
Beta strandi141 – 1455
Helixi149 – 16921

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RKBX-ray2.00A1-172[»]
3IIJX-ray1.76A1-172[»]
3IIKX-ray1.95A1-172[»]
3IILX-ray2.00A1-172[»]
3IIMX-ray2.00A1-172[»]
ProteinModelPortaliQ9Y3D8.
SMRiQ9Y3D8. Positions 1-172.

Miscellaneous databases

EvolutionaryTraceiQ9Y3D8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 5624NMPbindUniRule annotation
Add
BLAST
Regioni108 – 11811LIDUniRule annotation
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1936.
HOGENOMiHOG000224472.
HOVERGENiHBG052207.
InParanoidiQ9Y3D8.
KOiK14535.
OMAiDNVQCEI.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00039. Adenylate_kinase_AK6.
InterProiIPR020618. Adenyl_kinase_AK6.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Y3D8-1) [UniParc]FASTAAdd to Basket

Also known as: AK6

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLLPNILLTG TPGVGKTTLG KELASKSGLK YINVGDLARE EQLYDGYDEE    50
YDCPILDEDR VVDELDNQMR EGGVIVDYHG CDFFPERWFH IVFVLRTDTN 100
VLYERLETRG YNEKKLTDNI QCEIFQVLYE EATASYKEEI VHQLPSNKPE 150
ELENNVDQIL KWIEQWIKDH NS 172
Length:172
Mass (Da):20,061
Last modified:November 1, 1999 - v1
Checksum:i33F62318FB434301
GO
Isoform 2 (identifier: Q9Y3D8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-9: LLPNILLT → CHRKP

Note: Gene prediction based on EST data.

Show »
Length:169
Mass (Da):19,805
Checksum:i3F341AE6C5898C66
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 98LLPNILLT → CHRKP in isoform 2.
VSP_039714

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ878880 mRNA. Translation: CAI48030.1.
AJ878881 mRNA. Translation: CAI48031.1.
AF151895 mRNA. Translation: AAD34132.1.
AF110777 mRNA. Translation: AAF14860.1.
AC145132 Genomic DNA. No translation available.
CH471137 Genomic DNA. Translation: EAW51290.1.
CH471137 Genomic DNA. Translation: EAW51291.1.
CH471137 Genomic DNA. Translation: EAW51292.1.
CH471137 Genomic DNA. Translation: EAW51297.1.
BC007349 mRNA. Translation: AAH07349.1.
BC007426 mRNA. Translation: AAH07426.1.
CCDSiCCDS4001.1. [Q9Y3D8-1]
CCDS43324.1. [Q9Y3D8-2]
RefSeqiNP_001015891.1. NM_001015891.1. [Q9Y3D8-2]
NP_057367.1. NM_016283.4. [Q9Y3D8-1]
UniGeneiHs.653163.

Genome annotation databases

GeneIDi102157402.
KEGGihsa:102157402.
UCSCiuc003jwa.3. human. [Q9Y3D8-1]
uc003jwb.3. human. [Q9Y3D8-2]

Polymorphism databases

DMDMi6831735.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ878880 mRNA. Translation: CAI48030.1 .
AJ878881 mRNA. Translation: CAI48031.1 .
AF151895 mRNA. Translation: AAD34132.1 .
AF110777 mRNA. Translation: AAF14860.1 .
AC145132 Genomic DNA. No translation available.
CH471137 Genomic DNA. Translation: EAW51290.1 .
CH471137 Genomic DNA. Translation: EAW51291.1 .
CH471137 Genomic DNA. Translation: EAW51292.1 .
CH471137 Genomic DNA. Translation: EAW51297.1 .
BC007349 mRNA. Translation: AAH07349.1 .
BC007426 mRNA. Translation: AAH07426.1 .
CCDSi CCDS4001.1. [Q9Y3D8-1 ]
CCDS43324.1. [Q9Y3D8-2 ]
RefSeqi NP_001015891.1. NM_001015891.1. [Q9Y3D8-2 ]
NP_057367.1. NM_016283.4. [Q9Y3D8-1 ]
UniGenei Hs.653163.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RKB X-ray 2.00 A 1-172 [» ]
3IIJ X-ray 1.76 A 1-172 [» ]
3IIK X-ray 1.95 A 1-172 [» ]
3IIL X-ray 2.00 A 1-172 [» ]
3IIM X-ray 2.00 A 1-172 [» ]
ProteinModelPortali Q9Y3D8.
SMRi Q9Y3D8. Positions 1-172.
ModBasei Search...

Protein-protein interaction databases

BioGridi 112743. 70 interactions.
IntActi Q9Y3D8. 4 interactions.
MINTi MINT-1389230.
STRINGi 9606.ENSP00000370201.

Polymorphism databases

DMDMi 6831735.

Proteomic databases

MaxQBi Q9Y3D8.
PaxDbi Q9Y3D8.
PeptideAtlasi Q9Y3D8.
PRIDEi Q9Y3D8.

Protocols and materials databases

DNASUi 6880.
Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102157402.
KEGGi hsa:102157402.
UCSCi uc003jwa.3. human. [Q9Y3D8-1 ]
uc003jwb.3. human. [Q9Y3D8-2 ]

Organism-specific databases

CTDi 102157402.
GeneCardsi GC05M068646.
GC05M068647.
HGNCi HGNC:49151. AK6.
HPAi CAB005361.
neXtProti NX_Q9Y3D8.
PharmGKBi PA36317.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1936.
HOGENOMi HOG000224472.
HOVERGENi HBG052207.
InParanoidi Q9Y3D8.
KOi K14535.
OMAi DNVQCEI.

Miscellaneous databases

ChiTaRSi TAF9. human.
EvolutionaryTracei Q9Y3D8.
GenomeRNAii 102157402.
NextBioi 26873.

Gene expression databases

ArrayExpressi Q9Y3D8.
Bgeei Q9Y3D8.
Genevestigatori Q9Y3D8.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00039. Adenylate_kinase_AK6.
InterProi IPR020618. Adenyl_kinase_AK6.
IPR027417. P-loop_NTPase.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of hCINAP, a novel coilin-interacting protein encoded by a transcript from the transcription factor TAFIID32 locus."
    Santama N., Ogg S.C., Malekkou A., Zographos S.E., Weis K., Lamond A.I.
    J. Biol. Chem. 280:36429-36441(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH COIL, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    Tissue: Cervix carcinoma and Placenta.
  2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Adrenal gland.
  4. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  7. "Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004."
    Ren H., Liang Y., Li R., Ding H., Qiu S., Lu S., An J., Li L., Luo M., Zheng X., Su X.D.
    Acta Crystallogr. D 60:1292-1294(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "The crystal structure of human adenylate kinase 6: an adenylate kinase localized to the cell nucleus."
    Ren H., Wang L., Bennett M., Liang Y., Zheng X., Lu F., Li L., Nan J., Luo M., Eriksson S., Zhang C., Su X.-D.
    Proc. Natl. Acad. Sci. U.S.A. 102:303-308(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
  10. "hCINAP is an atypical mammalian nuclear adenylate kinase with an ATPase motif: structural and functional studies."
    Drakou C.E., Malekkou A., Hayes J.M., Lederer C.W., Leonidas D.D., Oikonomakos N.G., Lamond A.I., Santama N., Zographos S.E.
    Proteins 80:206-220(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) IN COMPLEX WITH ADP AND DADP, MUTAGENESIS OF HIS-79, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiKAD6_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3D8
Secondary accession number(s): A8MSZ6, Q5F2S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3

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