Q9Y3D7 (TIM16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM16

Alternative name(s):
Mitochondria-associated granulocyte macrophage CSF-signaling molecule
Presequence translocated-associated motor subunit PAM16

Gene names

Name:	PAM16
Synonyms:	MAGMAS, TIM16, TIMM16
ORF Names:	CGI-136

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity.
Subunit structure	Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 By similarity. Interacts with DNAJC19. Associates with the TIM23 complex.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.1.
Tissue specificity	Ubiquitously expressed. Ref.6
Induction	By CSF2/GM-CSF.
Domain	The J-like region, although related to the J domain does not have co-chaperone activity By similarity.
Sequence similarities	Belongs to the TIM16/PAM16 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 125	125	Mitochondrial import inner membrane translocase subunit TIM16		PRO_0000214078
Regions
	Region	58 – 110	53	J-like
Natural variations
	Natural variant	114	1	Q → K. Ref.1 Corresponds to variant rs11989 [ dbSNP | Ensembl ].		VAR_013764
Experimental info
	Mutagenesis	62	1	I → A, Q or W: Substantial loss of protein translocation into mitochondria in a heterologous system.
	Mutagenesis	85 – 87	3	DKS → HPD: No effect on protein translocation into mitochondria in a heterologous system.
	Mutagenesis	92	1	F → G: Partial loss of protein translocation into mitochondria in a heterologous system. Substantial loss of protein translocation into mitochondria in a heterologous system; when associated with G-93. Partial loss of DNAJC19-binding. Loss of DNAJC19-binding; when associated with G-93. Partial loss of inhibition of DNAJC19 stimulation of HSPA9 ATPase activity. Complete loss of inhibition of DNAJC19 stimulation of HSPA9 ATPase activity; when associated with G-93.
	Mutagenesis	93	1	Y → G: Partial loss of protein translocation into mitochondria in a heterologous system. Substantial loss of protein translocation into mitochondria in a heterologous system; when associated with G-92. Loss of DNAJC19-binding; when associated with G-92. Complete loss of inhibition of DNAJC19 stimulation of HSPA9 ATPase activity; when associated with G-92.
	Mutagenesis	94	1	L → A: No effect on protein translocation into mitochondria in a heterologous system.
	Mutagenesis	94	1	L → Q: Substantial loss of protein translocation into mitochondria in a heterologous system. Substantial loss of DNAJC19-binding. Partial loss of inhibition of DNAJC19 stimulation of HSPA9 ATPase activity.
	Sequence conflict	120	1	G → W in AAD34131. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Y3D7 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 5E7877B30CC89C61	FASTA	125	13,825
	10 20 30 40 50 60 MAKYLAQIIV MGVQVVGRAF ARALRQEFAA SRAAADARGR AGHRSAAASN LSGLSLQEAQ 70 80 90 100 110 120 QILNVSKLSP EEVQKNYEHL FKVNDKSVGG SFYLQSKVVR AKERLDEELK IQAQEDREKG QMPHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of Magmas, a novel mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction." Jubinsky P.T., Messer A., Bender J., Morris R.E., Ciraolo G.M., Witte D.P., Hawley R.G., Short M.K. Exp. Hematol. 29:1392-1402(2001) [PubMed: 11750097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, VARIANT LYS-114. Tissue: Peripheral blood.
[2]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[6]	"Magmas expression in neoplastic human prostate." Jubinsky P.T., Short M.K., Mutema G., Morris R.E., Ciraolo G.M., Li M. J. Mol. Histol. 36:69-75(2005) [PubMed: 15704001] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Role of Magmas in protein transport and human mitochondria biogenesis." Sinha D., Joshi N., Chittoor B., Samji P., D'Silva P. Hum. Mol. Genet. 19:1248-1262(2010) [PubMed: 20053669] [Abstract] Cited for: FUNCTION, INTERACTION WITH DNAJC19, ASSOCIATION WITH THE TIM23 COMPLEX, SUBCELLULAR LOCATION, MUTAGENESIS OF ILE-62; 85-ASP--SER-87; PHE-92; TYR-93 AND LEU-94.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF349455 mRNA. Translation: AAL57767.1. AF151894 mRNA. Translation: AAD34131.1. AK026514 mRNA. Translation: BAB15494.1. CR457362 mRNA. Translation: CAG33643.1. BC005024 mRNA. Translation: AAH05024.1.
IPI	IPI00218463.
RefSeq	NP_057153.8. NM_016069.9.
UniGene	Hs.437957.
3D structure databases
ProteinModelPortal	Q9Y3D7.
SMR	Q9Y3D7. Positions 54-113.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y3D7. 1 interaction.
MINT	MINT-1403487.
STRING	Q9Y3D7.
PTM databases
PhosphoSite	Q9Y3D7.
Polymorphism databases
DMDM	23503082.
Proteomic databases
PeptideAtlas	Q9Y3D7.
PRIDE	Q9Y3D7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000318059; ENSP00000315693; ENSG00000217930.
GeneID	51025.
KEGG	hsa:51025.
UCSC	uc002cwd.1. human.
Organism-specific databases
CTD	51025.
GeneCards	GC16M004357.
HGNC	HGNC:29679. PAM16.
MIM	614336. gene.
neXtProt	NX_Q9Y3D7.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20745.
GeneTree	ENSGT00390000012037.
HOGENOM	HBG734654.
HOVERGEN	HBG094040.
InParanoid	Q9Y3D7.
OMA	VMGAQVV.
OrthoDB	EOG4J9N1K.
PhylomeDB	Q9Y3D7.
Gene expression databases
ArrayExpress	Q9Y3D7.
Bgee	Q9Y3D7.
Genevestigator	Q9Y3D7.
Family and domain databases
InterPro	IPR005341. Protein_transpt. [Graphical view]
PANTHER	PTHR12388. Protein_transpt. 1 hit.
Pfam	PF03656. Pam16. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	53562.
SOURCE	Search...

Entry information

Entry name

TIM16_HUMAN

Accession

Primary (citable) accession number: Q9Y3D7
Secondary accession number(s): Q6I9Z3, Q9H5X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	September 19, 2002
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.