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Protein

Mitochondrial fission 1 protein

Gene

FIS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.7 Publications

GO - Molecular functioni

  • receptor binding Source: UniProtKB

GO - Biological processi

  • calcium-mediated signaling using intracellular calcium source Source: UniProtKB
  • mitochondrial fission Source: UniProtKB
  • mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
  • mitochondrial fusion Source: UniProtKB
  • mitochondrion morphogenesis Source: UniProtKB
  • mitophagy Source: UniProtKB
  • negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKB
  • peroxisome fission Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • positive regulation of cytosolic calcium ion concentration Source: UniProtKB
  • positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  • positive regulation of mitochondrial calcium ion concentration Source: UniProtKB
  • positive regulation of mitochondrial fission Source: UniProtKB
  • positive regulation of protein targeting to membrane Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • protein targeting to mitochondrion Source: UniProtKB
  • regulation of mitochondrion organization Source: UniProtKB
  • release of cytochrome c from mitochondria Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BioCyciZFISH:G66-31020-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial fission 1 protein
Alternative name(s):
FIS1 homolog
Short name:
hFis1
Tetratricopeptide repeat protein 11
Short name:
TPR repeat protein 11
Gene namesi
Name:FIS1
Synonyms:TTC11
ORF Names:CGI-135
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:21689. FIS1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 122CytoplasmicSequence analysisAdd BLAST122
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 152Mitochondrial intermembraneSequence analysis9

GO - Cellular componenti

  • endoplasmic reticulum Source: GOC
  • integral component of mitochondrial outer membrane Source: UniProtKB
  • integral component of peroxisomal membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • mitochondrion Source: UniProtKB
  • peroxisome Source: UniProtKB
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14L → P: Approximately 40% of cells display fragmented mitochondria. 1 Publication1
Mutagenesisi42L → P: Less than 15% of cells display fragmented mitochondria. 1 Publication1
Mutagenesisi58L → P: Less than 15% of cells display fragmented mitochondria. 1 Publication1
Mutagenesisi77L → P: Less than 15% of cells display fragmented mitochondria. Shows greatly reduced binding to DNM1L. 1 Publication1
Mutagenesisi91L → P: Less than 15% of cells display fragmented mitochondria. Shows greatly reduced binding to DNM1L. 1 Publication1
Mutagenesisi110L → P: Approximately 40% of cells display fragmented mitochondria. No change in binding to DNM1L. 1 Publication1
Mutagenesisi149K → A: Protein localizes to both mitochondrion and endoplasmic reticulum. Protein localizes to endoplasmic reticulum only; when associated with A-151. 1 Publication1
Mutagenesisi151K → A: Protein localizes to both mitochondrion and endoplasmic reticulum. Protein localizes to endoplasmic reticulum only; when associated with A-149. 1 Publication1

Organism-specific databases

DisGeNETi51024.
OpenTargetsiENSG00000214253.
PharmGKBiPA134984211.

Polymorphism and mutation databases

BioMutaiFIS1.
DMDMi33112470.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063931 – 152Mitochondrial fission 1 proteinAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei10PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by MARCH5.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y3D6.
MaxQBiQ9Y3D6.
PaxDbiQ9Y3D6.
PeptideAtlasiQ9Y3D6.
PRIDEiQ9Y3D6.
TopDownProteomicsiQ9Y3D6.

2D gel databases

UCD-2DPAGEQ9Y3D6.

PTM databases

iPTMnetiQ9Y3D6.
PhosphoSitePlusiQ9Y3D6.

Expressioni

Gene expression databases

BgeeiENSG00000214253.
CleanExiHS_FIS1.
ExpressionAtlasiQ9Y3D6. baseline and differential.
GenevisibleiQ9Y3D6. HS.

Organism-specific databases

HPAiHPA017430.

Interactioni

Subunit structurei

Interacts with DNM1L/DLP1 through the TPR region. Interacts with MARCH5. Interacts with MIEF1. Interacts with PEX11A, PEX11B and PEX11G (PubMed:20826455).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCAP31P515728EBI-3385283,EBI-77683
CCDC155Q8N6L03EBI-3385283,EBI-749265
MIEF1Q9NQG64EBI-3385283,EBI-740987
NOD2Q9HC292EBI-3385283,EBI-7445625

GO - Molecular functioni

  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119230. 27 interactors.
IntActiQ9Y3D6. 12 interactors.
MINTiMINT-2844167.
STRINGi9606.ENSP00000223136.

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 27Combined sources27
Helixi32 – 42Combined sources11
Beta strandi45 – 47Combined sources3
Helixi48 – 61Combined sources14
Turni62 – 64Combined sources3
Helixi67 – 83Combined sources17
Helixi87 – 100Combined sources14
Helixi105 – 120Combined sources16
Beta strandi129 – 133Combined sources5
Beta strandi142 – 144Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NZNX-ray2.00A1-123[»]
1PC2NMR-A1-145[»]
DisProtiDP00457.
ProteinModelPortaliQ9Y3D6.
SMRiQ9Y3D6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y3D6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati71 – 104TPRAdd BLAST34

Domaini

The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.

Sequence similaritiesi

Belongs to the FIS1 family.Curated
Contains 1 TPR repeat.Curated

Keywords - Domaini

TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3364. Eukaryota.
ENOG4111PPH. LUCA.
GeneTreeiENSGT00390000000592.
HOGENOMiHOG000165386.
HOVERGENiHBG081530.
InParanoidiQ9Y3D6.
KOiK17969.
OMAiEGYIGMA.
PhylomeDBiQ9Y3D6.
TreeFamiTF315180.

Family and domain databases

CDDicd12212. Fis1. 1 hit.
Gene3Di1.25.40.10. 1 hit.
InterProiIPR016543. Fis1.
IPR033745. Fis1_cytosol.
IPR028061. Fis1_TPR_C.
IPR028058. Fis1_TPR_N.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13247. PTHR13247. 1 hit.
PfamiPF14853. Fis1_TPR_C. 1 hit.
PF14852. Fis1_TPR_N. 1 hit.
[Graphical view]
PIRSFiPIRSF008835. TPR_repeat_11_Fis1. 1 hit.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y3D6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVLNELVS VEDLLKFEKK FQSEKAAGSV SKSTQFEYAW CLVRSKYNDD
60 70 80 90 100
IRKGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT
110 120 130 140 150
EPQNNQAKEL ERLIDKAMKK DGLVGMAIVG GMALGVAGLA GLIGLAVSKS

KS
Length:152
Mass (Da):16,938
Last modified:July 19, 2003 - v2
Checksum:i6E76EC02B3731A9B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45 – 46SK → TR in AAD34130 (PubMed:10810093).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151893 mRNA. Translation: AAD34130.1.
AC006329 Genomic DNA. Translation: AAP22366.1.
BC003540 mRNA. Translation: AAH03540.1.
BC009428 mRNA. Translation: AAH09428.1.
CCDSiCCDS43626.1.
RefSeqiNP_057152.2. NM_016068.2.
UniGeneiHs.423968.

Genome annotation databases

EnsembliENST00000223136; ENSP00000223136; ENSG00000214253.
GeneIDi51024.
KEGGihsa:51024.
UCSCiuc003uyj.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151893 mRNA. Translation: AAD34130.1.
AC006329 Genomic DNA. Translation: AAP22366.1.
BC003540 mRNA. Translation: AAH03540.1.
BC009428 mRNA. Translation: AAH09428.1.
CCDSiCCDS43626.1.
RefSeqiNP_057152.2. NM_016068.2.
UniGeneiHs.423968.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NZNX-ray2.00A1-123[»]
1PC2NMR-A1-145[»]
DisProtiDP00457.
ProteinModelPortaliQ9Y3D6.
SMRiQ9Y3D6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119230. 27 interactors.
IntActiQ9Y3D6. 12 interactors.
MINTiMINT-2844167.
STRINGi9606.ENSP00000223136.

PTM databases

iPTMnetiQ9Y3D6.
PhosphoSitePlusiQ9Y3D6.

Polymorphism and mutation databases

BioMutaiFIS1.
DMDMi33112470.

2D gel databases

UCD-2DPAGEQ9Y3D6.

Proteomic databases

EPDiQ9Y3D6.
MaxQBiQ9Y3D6.
PaxDbiQ9Y3D6.
PeptideAtlasiQ9Y3D6.
PRIDEiQ9Y3D6.
TopDownProteomicsiQ9Y3D6.

Protocols and materials databases

DNASUi51024.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223136; ENSP00000223136; ENSG00000214253.
GeneIDi51024.
KEGGihsa:51024.
UCSCiuc003uyj.5. human.

Organism-specific databases

CTDi51024.
DisGeNETi51024.
GeneCardsiFIS1.
HGNCiHGNC:21689. FIS1.
HPAiHPA017430.
MIMi609003. gene.
neXtProtiNX_Q9Y3D6.
OpenTargetsiENSG00000214253.
PharmGKBiPA134984211.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3364. Eukaryota.
ENOG4111PPH. LUCA.
GeneTreeiENSGT00390000000592.
HOGENOMiHOG000165386.
HOVERGENiHBG081530.
InParanoidiQ9Y3D6.
KOiK17969.
OMAiEGYIGMA.
PhylomeDBiQ9Y3D6.
TreeFamiTF315180.

Enzyme and pathway databases

BioCyciZFISH:G66-31020-MONOMER.

Miscellaneous databases

ChiTaRSiFIS1. human.
EvolutionaryTraceiQ9Y3D6.
GeneWikiiFIS1.
GenomeRNAii51024.
PROiQ9Y3D6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000214253.
CleanExiHS_FIS1.
ExpressionAtlasiQ9Y3D6. baseline and differential.
GenevisibleiQ9Y3D6. HS.

Family and domain databases

CDDicd12212. Fis1. 1 hit.
Gene3Di1.25.40.10. 1 hit.
InterProiIPR016543. Fis1.
IPR033745. Fis1_cytosol.
IPR028061. Fis1_TPR_C.
IPR028058. Fis1_TPR_N.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13247. PTHR13247. 1 hit.
PfamiPF14853. Fis1_TPR_C. 1 hit.
PF14852. Fis1_TPR_N. 1 hit.
[Graphical view]
PIRSFiPIRSF008835. TPR_repeat_11_Fis1. 1 hit.
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFIS1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3D6
Secondary accession number(s): Q9BTP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 19, 2003
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.