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Protein

Mitochondrial fission 1 protein

Gene

FIS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.7 Publications

GO - Molecular functioni

  • protein-containing complex binding Source: Ensembl
  • signaling receptor binding Source: UniProtKB

GO - Biological processi

  • autophagy of mitochondrion Source: UniProtKB
  • calcium-mediated signaling using intracellular calcium source Source: UniProtKB
  • cellular response to glucose stimulus Source: Ensembl
  • cellular response to peptide Source: Ensembl
  • cellular response to thapsigargin Source: Ensembl
  • cellular response to toxic substance Source: Ensembl
  • mitochondrial fission Source: UniProtKB
  • mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
  • mitochondrial fusion Source: UniProtKB
  • mitochondrion morphogenesis Source: UniProtKB
  • negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKB
  • peroxisome fission Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • positive regulation of cytosolic calcium ion concentration Source: UniProtKB
  • positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  • positive regulation of mitochondrial calcium ion concentration Source: UniProtKB
  • positive regulation of mitochondrial fission Source: UniProtKB
  • positive regulation of neuron apoptotic process Source: Ensembl
  • positive regulation of protein targeting to membrane Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • protein targeting to mitochondrion Source: UniProtKB
  • regulation of mitochondrion organization Source: UniProtKB
  • release of cytochrome c from mitochondria Source: UniProtKB
  • response to flavonoid Source: Ensembl
  • response to fluoride Source: Ensembl
  • response to hypobaric hypoxia Source: Ensembl
  • response to muscle activity Source: Ensembl
  • response to nutrient levels Source: Ensembl

Keywordsi

Biological processApoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial fission 1 protein
Alternative name(s):
FIS1 homolog
Short name:
hFis1
Tetratricopeptide repeat protein 11
Short name:
TPR repeat protein 11
Gene namesi
Name:FIS1
Synonyms:TTC11
ORF Names:CGI-135
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000214253.8
HGNCiHGNC:21689 FIS1
MIMi609003 gene
neXtProtiNX_Q9Y3D6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 122CytoplasmicSequence analysisAdd BLAST122
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 152Mitochondrial intermembraneSequence analysis9

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14L → P: Approximately 40% of cells display fragmented mitochondria. 1 Publication1
Mutagenesisi42L → P: Less than 15% of cells display fragmented mitochondria. 1 Publication1
Mutagenesisi58L → P: Less than 15% of cells display fragmented mitochondria. 1 Publication1
Mutagenesisi77L → P: Less than 15% of cells display fragmented mitochondria. Shows greatly reduced binding to DNM1L. 1 Publication1
Mutagenesisi91L → P: Less than 15% of cells display fragmented mitochondria. Shows greatly reduced binding to DNM1L. 1 Publication1
Mutagenesisi110L → P: Approximately 40% of cells display fragmented mitochondria. No change in binding to DNM1L. 1 Publication1
Mutagenesisi149K → A: Protein localizes to both mitochondrion and endoplasmic reticulum. Protein localizes to endoplasmic reticulum only; when associated with A-151. 1 Publication1
Mutagenesisi151K → A: Protein localizes to both mitochondrion and endoplasmic reticulum. Protein localizes to endoplasmic reticulum only; when associated with A-149. 1 Publication1

Organism-specific databases

DisGeNETi51024
OpenTargetsiENSG00000214253
PharmGKBiPA134984211

Polymorphism and mutation databases

BioMutaiFIS1
DMDMi33112470

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063931 – 152Mitochondrial fission 1 proteinAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei10PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by MARCH5.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y3D6
MaxQBiQ9Y3D6
PaxDbiQ9Y3D6
PeptideAtlasiQ9Y3D6
PRIDEiQ9Y3D6
TopDownProteomicsiQ9Y3D6

2D gel databases

UCD-2DPAGEiQ9Y3D6

PTM databases

iPTMnetiQ9Y3D6
PhosphoSitePlusiQ9Y3D6

Expressioni

Gene expression databases

BgeeiENSG00000214253
CleanExiHS_FIS1
ExpressionAtlasiQ9Y3D6 baseline and differential
GenevisibleiQ9Y3D6 HS

Organism-specific databases

HPAiHPA017430

Interactioni

Subunit structurei

Interacts with DNM1L/DLP1 through the TPR region. Interacts with MARCH5. Interacts with MIEF1. Interacts with PEX11A, PEX11B and PEX11G (PubMed:20826455).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • signaling receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119230, 54 interactors
CORUMiQ9Y3D6
IntActiQ9Y3D6, 13 interactors
MINTiQ9Y3D6
STRINGi9606.ENSP00000223136

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 27Combined sources27
Helixi32 – 42Combined sources11
Beta strandi45 – 47Combined sources3
Helixi48 – 61Combined sources14
Turni62 – 64Combined sources3
Helixi67 – 83Combined sources17
Helixi87 – 100Combined sources14
Helixi105 – 120Combined sources16
Beta strandi129 – 133Combined sources5
Beta strandi142 – 144Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NZNX-ray2.00A1-123[»]
1PC2NMR-A1-145[»]
DisProtiDP00457
ProteinModelPortaliQ9Y3D6
SMRiQ9Y3D6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y3D6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati71 – 104TPRAdd BLAST34

Domaini

The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.

Sequence similaritiesi

Belongs to the FIS1 family.Curated

Keywords - Domaini

TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3364 Eukaryota
ENOG4111PPH LUCA
GeneTreeiENSGT00390000000592
HOGENOMiHOG000165386
HOVERGENiHBG081530
InParanoidiQ9Y3D6
KOiK17969
OMAiEMVGVQT
PhylomeDBiQ9Y3D6
TreeFamiTF315180

Family and domain databases

CDDicd12212 Fis1, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR016543 Fis1
IPR033745 Fis1_cytosol
IPR028061 Fis1_TPR_C
IPR028058 Fis1_TPR_N
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR13247 PTHR13247, 1 hit
PfamiView protein in Pfam
PF14853 Fis1_TPR_C, 1 hit
PF14852 Fis1_TPR_N, 1 hit
PIRSFiPIRSF008835 TPR_repeat_11_Fis1, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Y3D6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVLNELVS VEDLLKFEKK FQSEKAAGSV SKSTQFEYAW CLVRSKYNDD
60 70 80 90 100
IRKGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT
110 120 130 140 150
EPQNNQAKEL ERLIDKAMKK DGLVGMAIVG GMALGVAGLA GLIGLAVSKS

KS
Length:152
Mass (Da):16,938
Last modified:July 19, 2003 - v2
Checksum:i6E76EC02B3731A9B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45 – 46SK → TR in AAD34130 (PubMed:10810093).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151893 mRNA Translation: AAD34130.1
AC006329 Genomic DNA Translation: AAP22366.1
BC003540 mRNA Translation: AAH03540.1
BC009428 mRNA Translation: AAH09428.1
CCDSiCCDS43626.1
RefSeqiNP_057152.2, NM_016068.2
UniGeneiHs.423968

Genome annotation databases

EnsembliENST00000223136; ENSP00000223136; ENSG00000214253
GeneIDi51024
KEGGihsa:51024
UCSCiuc003uyj.5 human

Similar proteinsi

Entry informationi

Entry nameiFIS1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3D6
Secondary accession number(s): Q9BTP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 19, 2003
Last modified: May 23, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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