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Protein

Mitotic spindle-associated MMXD complex subunit MIP18

Gene

FAM96B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD.1 Publication

GO - Biological processi

  • chromosome segregation Source: UniProtKB
  • iron-sulfur cluster assembly Source: GO_Central
  • small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Chromosome partition

Enzyme and pathway databases

ReactomeiREACT_160176. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle-associated MMXD complex subunit MIP18
Alternative name(s):
MSS19-interacting protein of 18 kDa
Protein FAM96B
Gene namesi
Name:FAM96B
Synonyms:MIP18
ORF Names:CGI-128, HSPC118
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:24261. FAM96B.

Subcellular locationi

GO - Cellular componenti

  • CIA complex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • MMXD complex Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671830.

Polymorphism and mutation databases

BioMutaiFAM96B.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 163162Mitotic spindle-associated MMXD complex subunit MIP18PRO_0000212691Add
BLAST

Proteomic databases

MaxQBiQ9Y3D0.
PaxDbiQ9Y3D0.
PRIDEiQ9Y3D0.

PTM databases

PhosphoSiteiQ9Y3D0.

Expressioni

Gene expression databases

BgeeiQ9Y3D0.
CleanExiHS_FAM96B.
ExpressionAtlasiQ9Y3D0. baseline and differential.
GenevisibleiQ9Y3D0. HS.

Organism-specific databases

HPAiHPA041501.
HPA041736.

Interactioni

Subunit structurei

Component of the CIA complex. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with ERCC2 and MMS19; the interaction is direct.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIAO1O760717EBI-744045,EBI-725145

Protein-protein interaction databases

BioGridi119656. 64 interactions.
IntActiQ9Y3D0. 4 interactions.
MINTiMINT-5006739.
STRINGi9606.ENSP00000387471.

Structurei

3D structure databases

ProteinModelPortaliQ9Y3D0.
SMRiQ9Y3D0. Positions 43-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIP18 family.Curated

Phylogenomic databases

eggNOGiCOG5133.
GeneTreeiENSGT00390000017697.
HOVERGENiHBG029509.
InParanoidiQ9Y3D0.
OMAiRFKIDVH.
OrthoDBiEOG7034J5.
PhylomeDBiQ9Y3D0.
TreeFamiTF105940.

Family and domain databases

InterProiIPR002744. DUF59.
[Graphical view]
PfamiPF01883. DUF59. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y3D0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGGGGVGGG LLENANPLIY QRSGERPVTA GEEDEQVPDS IDAREIFDLI
60 70 80 90 100
RSINDPEHPL TLEELNVVEQ VRVQVSDPES TVAVAFTPTI PHCSMATLIG
110 120 130 140 150
LSIKVKLLRS LPQRFKMDVH ITPGTHASEH AVNKQLADKE RVAAALENTH
160
LLEVVNQCLS ARS
Length:163
Mass (Da):17,663
Last modified:November 1, 1999 - v1
Checksum:i323004BAB92B1486
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151886 mRNA. Translation: AAD34123.1.
AF161467 mRNA. Translation: AAF29082.1.
BC001733 mRNA. Translation: AAH01733.1.
BC005023 mRNA. Translation: AAH05023.1.
CCDSiCCDS45506.1.
RefSeqiNP_057146.1. NM_016062.3.
UniGeneiHs.9825.

Genome annotation databases

EnsembliENST00000422424; ENSP00000387471; ENSG00000166595.
GeneIDi51647.
KEGGihsa:51647.
UCSCiuc021tjy.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151886 mRNA. Translation: AAD34123.1.
AF161467 mRNA. Translation: AAF29082.1.
BC001733 mRNA. Translation: AAH01733.1.
BC005023 mRNA. Translation: AAH05023.1.
CCDSiCCDS45506.1.
RefSeqiNP_057146.1. NM_016062.3.
UniGeneiHs.9825.

3D structure databases

ProteinModelPortaliQ9Y3D0.
SMRiQ9Y3D0. Positions 43-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119656. 64 interactions.
IntActiQ9Y3D0. 4 interactions.
MINTiMINT-5006739.
STRINGi9606.ENSP00000387471.

PTM databases

PhosphoSiteiQ9Y3D0.

Polymorphism and mutation databases

BioMutaiFAM96B.

Proteomic databases

MaxQBiQ9Y3D0.
PaxDbiQ9Y3D0.
PRIDEiQ9Y3D0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000422424; ENSP00000387471; ENSG00000166595.
GeneIDi51647.
KEGGihsa:51647.
UCSCiuc021tjy.2. human.

Organism-specific databases

CTDi51647.
GeneCardsiGC16M066965.
HGNCiHGNC:24261. FAM96B.
HPAiHPA041501.
HPA041736.
MIMi614778. gene.
neXtProtiNX_Q9Y3D0.
PharmGKBiPA142671830.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5133.
GeneTreeiENSGT00390000017697.
HOVERGENiHBG029509.
InParanoidiQ9Y3D0.
OMAiRFKIDVH.
OrthoDBiEOG7034J5.
PhylomeDBiQ9Y3D0.
TreeFamiTF105940.

Enzyme and pathway databases

ReactomeiREACT_160176. Cytosolic iron-sulfur cluster assembly.

Miscellaneous databases

ChiTaRSiFAM96B. human.
GenomeRNAii51647.
NextBioi55596.
PROiQ9Y3D0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y3D0.
CleanExiHS_FAM96B.
ExpressionAtlasiQ9Y3D0. baseline and differential.
GenevisibleiQ9Y3D0. HS.

Family and domain databases

InterProiIPR002744. DUF59.
[Graphical view]
PfamiPF01883. DUF59. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye and Uterus.
  4. Bienvenut W.V., Waridel P., Quadroni M.
    Submitted (MAR-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-22; 117-134 AND 142-162, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryonic kidney.
  5. "MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation."
    Ito S., Tan L.J., Andoh D., Narita T., Seki M., Hirano Y., Narita K., Kuraoka I., Hiraoka Y., Tanaka K.
    Mol. Cell 39:632-640(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN MMXD COMPLEX, INTERACTION WITH ERCC2 AND MMS19, SUBCELLULAR LOCATION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity."
    Stehling O., Vashisht A.A., Mascarenhas J., Jonsson Z.O., Sharma T., Netz D.J., Pierik A.J., Wohlschlegel J.A., Lill R.
    Science 337:195-199(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CIA COMPLEX.
  9. "MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism."
    Gari K., Leon Ortiz A.M., Borel V., Flynn H., Skehel J.M., Boulton S.J.
    Science 337:243-245(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CIA COMPLEX.
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMIP18_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1999
Last modified: June 24, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.