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Protein

Ubiquitin-fold modifier-conjugating enzyme 1

Gene

UFC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei116 – 1161Glycyl thioester intermediate

GO - Molecular functioni

  • UFM1 transferase activity Source: UniProtKB

GO - Biological processi

  • protein K69-linked ufmylation Source: UniProtKB
  • protein ufmylation Source: UniProtKB
  • response to endoplasmic reticulum stress Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-fold modifier-conjugating enzyme 1
Short name:
Ufm1-conjugating enzyme 1
Gene namesi
Name:UFC1
ORF Names:CGI-126, HSPC155
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26941. UFC1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301Q → A: Does not affect neither UBA5-binding nor thioester formation with UFM1. 1 Publication
Mutagenesisi33 – 331K → A: Impairs binding to UBA5 and thioester formation with UFM1. 1 Publication
Mutagenesisi116 – 1161C → S: Instead of the formation of an intermediate complex with a thiol ester bond between UFC1 (E2-like enzyme) and UFM1 (substrate), a stable complex with an O-ester bond is formed. 1 Publication

Organism-specific databases

PharmGKBiPA142670644.

Polymorphism and mutation databases

BioMutaiUFC1.
DMDMi116242840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 167167Ubiquitin-fold modifier-conjugating enzyme 1PRO_0000082613Add
BLAST

Proteomic databases

MaxQBiQ9Y3C8.
PaxDbiQ9Y3C8.
PeptideAtlasiQ9Y3C8.
PRIDEiQ9Y3C8.

PTM databases

PhosphoSiteiQ9Y3C8.

Expressioni

Gene expression databases

BgeeiQ9Y3C8.
CleanExiHS_UFC1.
GenevisibleiQ9Y3C8. HS.

Organism-specific databases

HPAiHPA027481.
HPA028722.

Interactioni

Subunit structurei

Interacts with UBA5. Interacts with UFL1.2 Publications

Protein-protein interaction databases

BioGridi119577. 27 interactions.
IntActiQ9Y3C8. 2 interactions.
STRINGi9606.ENSP00000356982.

Structurei

Secondary structure

1
167
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 109Combined sources
Helixi26 – 4823Combined sources
Beta strandi54 – 585Combined sources
Beta strandi64 – 7310Combined sources
Beta strandi76 – 8510Combined sources
Turni88 – 925Combined sources
Helixi100 – 1023Combined sources
Turni103 – 1053Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi113 – 1153Combined sources
Helixi121 – 1288Combined sources
Helixi134 – 1407Combined sources
Helixi142 – 15615Combined sources
Beta strandi162 – 1643Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K07NMR-A1-167[»]
2Z6OX-ray1.60A1-167[»]
2Z6PX-ray1.80A1-167[»]
3EVXX-ray2.54A/B/C/D1-167[»]
ProteinModelPortaliQ9Y3C8.
SMRiQ9Y3C8. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y3C8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG318908.
GeneTreeiENSGT00390000008196.
HOVERGENiHBG054254.
InParanoidiQ9Y3C8.
KOiK12165.
OMAiEHFKPLW.
OrthoDBiEOG7JMGFR.
PhylomeDBiQ9Y3C8.
TreeFamiTF313587.

Family and domain databases

InterProiIPR016135. UBQ-conjugating_enzyme/RWD.
IPR014806. Ufc1.
[Graphical view]
PANTHERiPTHR12921. PTHR12921. 1 hit.
PfamiPF08694. UFC1. 1 hit.
[Graphical view]
PIRSFiPIRSF008716. DUF1782. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y3C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD
60 70 80 90 100
NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP
110 120 130 140 150
ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI
160
PDLIQKGVIQ HKEKCNQ
Length:167
Mass (Da):19,458
Last modified:October 17, 2006 - v3
Checksum:i1675D9187DC43E14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 1591I → N in BAF85465 (PubMed:14702039).Curated
Sequence conflicti160 – 1601Q → H in BAD15374 (PubMed:15071506).Curated
Sequence conflicti160 – 1601Q → H in AAF29119 (PubMed:11042152).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti90 – 901Y → C.1 Publication
Corresponds to variant rs17849932 [ dbSNP | Ensembl ].
VAR_028312

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154405 mRNA. Translation: BAD15374.1.
AF151884 mRNA. Translation: AAD34121.1.
AF161504 mRNA. Translation: AAF29119.1.
AK292776 mRNA. Translation: BAF85465.1.
AL590714 Genomic DNA. Translation: CAH72141.1.
CH471121 Genomic DNA. Translation: EAW52646.1.
CH471121 Genomic DNA. Translation: EAW52647.1.
BC005187 mRNA. Translation: AAH05187.1.
CCDSiCCDS1220.1.
RefSeqiNP_057490.2. NM_016406.3.
UniGeneiHs.301412.

Genome annotation databases

EnsembliENST00000368003; ENSP00000356982; ENSG00000143222.
GeneIDi51506.
KEGGihsa:51506.
UCSCiuc001fyd.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154405 mRNA. Translation: BAD15374.1.
AF151884 mRNA. Translation: AAD34121.1.
AF161504 mRNA. Translation: AAF29119.1.
AK292776 mRNA. Translation: BAF85465.1.
AL590714 Genomic DNA. Translation: CAH72141.1.
CH471121 Genomic DNA. Translation: EAW52646.1.
CH471121 Genomic DNA. Translation: EAW52647.1.
BC005187 mRNA. Translation: AAH05187.1.
CCDSiCCDS1220.1.
RefSeqiNP_057490.2. NM_016406.3.
UniGeneiHs.301412.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K07NMR-A1-167[»]
2Z6OX-ray1.60A1-167[»]
2Z6PX-ray1.80A1-167[»]
3EVXX-ray2.54A/B/C/D1-167[»]
ProteinModelPortaliQ9Y3C8.
SMRiQ9Y3C8. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119577. 27 interactions.
IntActiQ9Y3C8. 2 interactions.
STRINGi9606.ENSP00000356982.

PTM databases

PhosphoSiteiQ9Y3C8.

Polymorphism and mutation databases

BioMutaiUFC1.
DMDMi116242840.

Proteomic databases

MaxQBiQ9Y3C8.
PaxDbiQ9Y3C8.
PeptideAtlasiQ9Y3C8.
PRIDEiQ9Y3C8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368003; ENSP00000356982; ENSG00000143222.
GeneIDi51506.
KEGGihsa:51506.
UCSCiuc001fyd.4. human.

Organism-specific databases

CTDi51506.
GeneCardsiGC01P161122.
HGNCiHGNC:26941. UFC1.
HPAiHPA027481.
HPA028722.
MIMi610554. gene.
neXtProtiNX_Q9Y3C8.
PharmGKBiPA142670644.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG318908.
GeneTreeiENSGT00390000008196.
HOVERGENiHBG054254.
InParanoidiQ9Y3C8.
KOiK12165.
OMAiEHFKPLW.
OrthoDBiEOG7JMGFR.
PhylomeDBiQ9Y3C8.
TreeFamiTF313587.

Miscellaneous databases

ChiTaRSiUFC1. human.
EvolutionaryTraceiQ9Y3C8.
GenomeRNAii51506.
NextBioi55190.
PROiQ9Y3C8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y3C8.
CleanExiHS_UFC1.
GenevisibleiQ9Y3C8. HS.

Family and domain databases

InterProiIPR016135. UBQ-conjugating_enzyme/RWD.
IPR014806. Ufc1.
[Graphical view]
PANTHERiPTHR12921. PTHR12921. 1 hit.
PfamiPF08694. UFC1. 1 hit.
[Graphical view]
PIRSFiPIRSF008716. DUF1782. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier."
    Komatsu M., Chiba T., Tatsumi K., Iemura S., Tanida I., Okazaki N., Ueno T., Kominami E., Natsume T., Tanaka K.
    EMBO J. 23:1977-1986(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-116.
  2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Trachea.
  5. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-90.
    Tissue: Urinary bladder.
  8. Cited for: INTERACTION WITH UFL1.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "GFT NMR based resonance assignment for the 21 kDa human protein UFC1."
    Liu G., Aramini J., Atreya H.S., Eletsky A., Xiao R., Acton T., Ma L., Montelione G.T., Szyperski T.
    J. Biomol. NMR 32:261-261(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY STRUCTURE BY NMR.
  11. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), INTERACTION WITH UBA5, MUTAGENESIS OF GLN-30 AND LYS-33.
  12. "NMR and X-RAY structures of human E2-like ubiquitin-fold modifier conjugating enzyme 1 (UFC1) reveal structural and functional conservation in the metazoan UFM1-UBA5-UFC1 ubiquination pathway."
    Liu G., Forouhar F., Eletsky A., Atreya H.S., Aramini J.M., Xiao R., Huang Y.J., Abashidze M., Seetharaman J., Liu J., Rost B., Acton T., Montelione G.T., Hunt J.F., Szyperski T.
    J. Struct. Funct. Genomics 10:127-136(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.54 ANGSTROMS), STRUCTURE BY NMR.

Entry informationi

Entry nameiUFC1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3C8
Secondary accession number(s): A8K9R1
, D3DVF9, Q549X0, Q5VTX1, Q9BS96, Q9P009
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 17, 2006
Last modified: June 24, 2015
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.