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Q9Y3C8

- UFC1_HUMAN

UniProt

Q9Y3C8 - UFC1_HUMAN

Protein

Ubiquitin-fold modifier-conjugating enzyme 1

Gene

UFC1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 3 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei116 – 1161Glycyl thioester intermediate

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. UFM1 conjugating enzyme activity Source: UniProtKB

    GO - Biological processi

    1. protein ufmylation Source: UniProtKB
    2. response to endoplasmic reticulum stress Source: MGI

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-fold modifier-conjugating enzyme 1
    Short name:
    Ufm1-conjugating enzyme 1
    Gene namesi
    Name:UFC1
    ORF Names:CGI-126, HSPC155
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:26941. UFC1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi30 – 301Q → A: Does not affect neither UBA5-binding nor thioester formation with UFM1. 1 Publication
    Mutagenesisi33 – 331K → A: Impairs binding to UBA5 and thioester formation with UFM1. 1 Publication
    Mutagenesisi116 – 1161C → S: Instead of the formation of an intermediate complex with a thiol ester bond between UFC1 (E2-like enzyme) and UFM1 (substrate), a stable complex with an O-ester bond is formed. 1 Publication

    Organism-specific databases

    PharmGKBiPA142670644.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 167167Ubiquitin-fold modifier-conjugating enzyme 1PRO_0000082613Add
    BLAST

    Proteomic databases

    MaxQBiQ9Y3C8.
    PaxDbiQ9Y3C8.
    PeptideAtlasiQ9Y3C8.
    PRIDEiQ9Y3C8.

    PTM databases

    PhosphoSiteiQ9Y3C8.

    Expressioni

    Gene expression databases

    BgeeiQ9Y3C8.
    CleanExiHS_UFC1.
    GenevestigatoriQ9Y3C8.

    Organism-specific databases

    HPAiHPA027481.
    HPA028722.

    Interactioni

    Subunit structurei

    Interacts with UBA5. Interacts with UFL1.2 Publications

    Protein-protein interaction databases

    BioGridi119577. 26 interactions.
    IntActiQ9Y3C8. 1 interaction.
    STRINGi9606.ENSP00000356982.

    Structurei

    Secondary structure

    1
    167
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 109
    Helixi26 – 4823
    Beta strandi54 – 585
    Beta strandi64 – 7310
    Beta strandi76 – 8510
    Turni88 – 925
    Helixi100 – 1023
    Turni103 – 1053
    Beta strandi107 – 1093
    Beta strandi113 – 1153
    Helixi121 – 1288
    Helixi134 – 1407
    Helixi142 – 15615
    Beta strandi162 – 1643

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2K07NMR-A1-167[»]
    2Z6OX-ray1.60A1-167[»]
    2Z6PX-ray1.80A1-167[»]
    3EVXX-ray2.54A/B/C/D1-167[»]
    ProteinModelPortaliQ9Y3C8.
    SMRiQ9Y3C8. Positions 1-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9Y3C8.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG318908.
    HOVERGENiHBG054254.
    InParanoidiQ9Y3C8.
    KOiK12165.
    OMAiGTKWFGK.
    OrthoDBiEOG7JMGFR.
    PhylomeDBiQ9Y3C8.
    TreeFamiTF313587.

    Family and domain databases

    InterProiIPR016135. UBQ-conjugating_enzyme/RWD.
    IPR014806. Ufc1.
    [Graphical view]
    PANTHERiPTHR12921. PTHR12921. 1 hit.
    PfamiPF08694. UFC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF008716. DUF1782. 1 hit.
    SUPFAMiSSF54495. SSF54495. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9Y3C8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD    50
    NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP 100
    ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI 150
    PDLIQKGVIQ HKEKCNQ 167
    Length:167
    Mass (Da):19,458
    Last modified:October 17, 2006 - v3
    Checksum:i1675D9187DC43E14
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti159 – 1591I → N in BAF85465. (PubMed:14702039)Curated
    Sequence conflicti160 – 1601Q → H in BAD15374. (PubMed:15071506)Curated
    Sequence conflicti160 – 1601Q → H in AAF29119. (PubMed:11042152)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti90 – 901Y → C.1 Publication
    Corresponds to variant rs17849932 [ dbSNP | Ensembl ].
    VAR_028312

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB154405 mRNA. Translation: BAD15374.1.
    AF151884 mRNA. Translation: AAD34121.1.
    AF161504 mRNA. Translation: AAF29119.1.
    AK292776 mRNA. Translation: BAF85465.1.
    AL590714 Genomic DNA. Translation: CAH72141.1.
    CH471121 Genomic DNA. Translation: EAW52646.1.
    CH471121 Genomic DNA. Translation: EAW52647.1.
    BC005187 mRNA. Translation: AAH05187.1.
    CCDSiCCDS1220.1.
    RefSeqiNP_057490.2. NM_016406.3.
    UniGeneiHs.301412.

    Genome annotation databases

    EnsembliENST00000368003; ENSP00000356982; ENSG00000143222.
    GeneIDi51506.
    KEGGihsa:51506.
    UCSCiuc001fyd.4. human.

    Polymorphism databases

    DMDMi116242840.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB154405 mRNA. Translation: BAD15374.1 .
    AF151884 mRNA. Translation: AAD34121.1 .
    AF161504 mRNA. Translation: AAF29119.1 .
    AK292776 mRNA. Translation: BAF85465.1 .
    AL590714 Genomic DNA. Translation: CAH72141.1 .
    CH471121 Genomic DNA. Translation: EAW52646.1 .
    CH471121 Genomic DNA. Translation: EAW52647.1 .
    BC005187 mRNA. Translation: AAH05187.1 .
    CCDSi CCDS1220.1.
    RefSeqi NP_057490.2. NM_016406.3.
    UniGenei Hs.301412.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2K07 NMR - A 1-167 [» ]
    2Z6O X-ray 1.60 A 1-167 [» ]
    2Z6P X-ray 1.80 A 1-167 [» ]
    3EVX X-ray 2.54 A/B/C/D 1-167 [» ]
    ProteinModelPortali Q9Y3C8.
    SMRi Q9Y3C8. Positions 1-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119577. 26 interactions.
    IntActi Q9Y3C8. 1 interaction.
    STRINGi 9606.ENSP00000356982.

    PTM databases

    PhosphoSitei Q9Y3C8.

    Polymorphism databases

    DMDMi 116242840.

    Proteomic databases

    MaxQBi Q9Y3C8.
    PaxDbi Q9Y3C8.
    PeptideAtlasi Q9Y3C8.
    PRIDEi Q9Y3C8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000368003 ; ENSP00000356982 ; ENSG00000143222 .
    GeneIDi 51506.
    KEGGi hsa:51506.
    UCSCi uc001fyd.4. human.

    Organism-specific databases

    CTDi 51506.
    GeneCardsi GC01P161122.
    HGNCi HGNC:26941. UFC1.
    HPAi HPA027481.
    HPA028722.
    MIMi 610554. gene.
    neXtProti NX_Q9Y3C8.
    PharmGKBi PA142670644.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG318908.
    HOVERGENi HBG054254.
    InParanoidi Q9Y3C8.
    KOi K12165.
    OMAi GTKWFGK.
    OrthoDBi EOG7JMGFR.
    PhylomeDBi Q9Y3C8.
    TreeFami TF313587.

    Miscellaneous databases

    ChiTaRSi UFC1. human.
    EvolutionaryTracei Q9Y3C8.
    GenomeRNAii 51506.
    NextBioi 55190.
    PROi Q9Y3C8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9Y3C8.
    CleanExi HS_UFC1.
    Genevestigatori Q9Y3C8.

    Family and domain databases

    InterProi IPR016135. UBQ-conjugating_enzyme/RWD.
    IPR014806. Ufc1.
    [Graphical view ]
    PANTHERi PTHR12921. PTHR12921. 1 hit.
    Pfami PF08694. UFC1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF008716. DUF1782. 1 hit.
    SUPFAMi SSF54495. SSF54495. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier."
      Komatsu M., Chiba T., Tatsumi K., Iemura S., Tanida I., Okazaki N., Ueno T., Kominami E., Natsume T., Tanaka K.
      EMBO J. 23:1977-1986(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-116.
    2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
      Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
      Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Umbilical cord blood.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Trachea.
    5. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-90.
      Tissue: Urinary bladder.
    8. Cited for: INTERACTION WITH UFL1.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "GFT NMR based resonance assignment for the 21 kDa human protein UFC1."
      Liu G., Aramini J., Atreya H.S., Eletsky A., Xiao R., Acton T., Ma L., Montelione G.T., Szyperski T.
      J. Biomol. NMR 32:261-261(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY STRUCTURE BY NMR.
    11. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), INTERACTION WITH UBA5, MUTAGENESIS OF GLN-30 AND LYS-33.
    12. "NMR and X-RAY structures of human E2-like ubiquitin-fold modifier conjugating enzyme 1 (UFC1) reveal structural and functional conservation in the metazoan UFM1-UBA5-UFC1 ubiquination pathway."
      Liu G., Forouhar F., Eletsky A., Atreya H.S., Aramini J.M., Xiao R., Huang Y.J., Abashidze M., Seetharaman J., Liu J., Rost B., Acton T., Montelione G.T., Hunt J.F., Szyperski T.
      J. Struct. Funct. Genomics 10:127-136(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.54 ANGSTROMS), STRUCTURE BY NMR.

    Entry informationi

    Entry nameiUFC1_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y3C8
    Secondary accession number(s): A8K9R1
    , D3DVF9, Q549X0, Q5VTX1, Q9BS96, Q9P009
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3