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Q9Y3C7

- MED31_HUMAN

UniProt

Q9Y3C7 - MED31_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 31

Gene

MED31

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA polymerase II transcription cofactor activity Source: InterPro

    GO - Biological processi

    1. gene expression Source: Reactome
    2. limb development Source: Ensembl
    3. negative regulation of fibroblast proliferation Source: Ensembl
    4. protein complex assembly Source: Ensembl
    5. transcription initiation from RNA polymerase II promoter Source: Reactome

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 31
    Alternative name(s):
    Mediator complex subunit 31
    Mediator complex subunit SOH1
    Short name:
    hSOH1
    Gene namesi
    Name:MED31
    Synonyms:SOH1
    ORF Names:CGI-125
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:24260. MED31.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: InterPro
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134884310.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 131130Mediator of RNA polymerase II transcription subunit 31PRO_0000212527Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9Y3C7.
    PaxDbiQ9Y3C7.
    PRIDEiQ9Y3C7.

    PTM databases

    PhosphoSiteiQ9Y3C7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Y3C7.
    BgeeiQ9Y3C7.
    CleanExiHS_MED31.
    GenevestigatoriQ9Y3C7.

    Organism-specific databases

    HPAiHPA035947.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GADD45GO952572EBI-394707,EBI-448202
    HTTP428583EBI-394707,EBI-466029

    Protein-protein interaction databases

    BioGridi119211. 69 interactions.
    IntActiQ9Y3C7. 60 interactions.
    MINTiMINT-1418286.
    STRINGi9606.ENSP00000225728.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y3C7.
    SMRiQ9Y3C7. Positions 17-79.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi122 – 1254Poly-Gln

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 31 family.Curated

    Phylogenomic databases

    eggNOGiCOG5088.
    HOGENOMiHOG000242147.
    HOVERGENiHBG052449.
    InParanoidiQ9Y3C7.
    KOiK15153.
    OMAiSDDTGNR.
    OrthoDBiEOG761BWW.
    PhylomeDBiQ9Y3C7.
    TreeFamiTF105799.

    Family and domain databases

    InterProiIPR008831. Mediator_Med31.
    [Graphical view]
    PANTHERiPTHR13186. PTHR13186. 1 hit.
    PfamiPF05669. Med31. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9Y3C7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN    50
    YLKYLLYWKD PEYAKYLKYP QCLHMLELLQ YEHFRKELVN AQCAKFIDEQ 100
    QILHWQHYSR KRMRLQQALA EQQQQNNTSG K 131
    Length:131
    Mass (Da):15,805
    Last modified:November 1, 1999 - v1
    Checksum:i5DC672ADB994D015
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF151883 mRNA. Translation: AAD34120.1.
    AK311875 mRNA. Translation: BAG34816.1.
    CH471108 Genomic DNA. Translation: EAW90301.1.
    BC012539 mRNA. Translation: AAH12539.1.
    CCDSiCCDS11078.1.
    RefSeqiNP_057144.1. NM_016060.2.
    UniGeneiHs.567493.

    Genome annotation databases

    EnsembliENST00000225728; ENSP00000225728; ENSG00000108590.
    GeneIDi51003.
    KEGGihsa:51003.
    UCSCiuc002gdg.4. human.

    Polymorphism databases

    DMDMi38258656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF151883 mRNA. Translation: AAD34120.1 .
    AK311875 mRNA. Translation: BAG34816.1 .
    CH471108 Genomic DNA. Translation: EAW90301.1 .
    BC012539 mRNA. Translation: AAH12539.1 .
    CCDSi CCDS11078.1.
    RefSeqi NP_057144.1. NM_016060.2.
    UniGenei Hs.567493.

    3D structure databases

    ProteinModelPortali Q9Y3C7.
    SMRi Q9Y3C7. Positions 17-79.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119211. 69 interactions.
    IntActi Q9Y3C7. 60 interactions.
    MINTi MINT-1418286.
    STRINGi 9606.ENSP00000225728.

    PTM databases

    PhosphoSitei Q9Y3C7.

    Polymorphism databases

    DMDMi 38258656.

    Proteomic databases

    MaxQBi Q9Y3C7.
    PaxDbi Q9Y3C7.
    PRIDEi Q9Y3C7.

    Protocols and materials databases

    DNASUi 51003.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000225728 ; ENSP00000225728 ; ENSG00000108590 .
    GeneIDi 51003.
    KEGGi hsa:51003.
    UCSCi uc002gdg.4. human.

    Organism-specific databases

    CTDi 51003.
    GeneCardsi GC17M006546.
    HGNCi HGNC:24260. MED31.
    HPAi HPA035947.
    neXtProti NX_Q9Y3C7.
    PharmGKBi PA134884310.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5088.
    HOGENOMi HOG000242147.
    HOVERGENi HBG052449.
    InParanoidi Q9Y3C7.
    KOi K15153.
    OMAi SDDTGNR.
    OrthoDBi EOG761BWW.
    PhylomeDBi Q9Y3C7.
    TreeFami TF105799.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    GeneWikii MED31.
    GenomeRNAii 51003.
    NextBioi 53464.
    PROi Q9Y3C7.

    Gene expression databases

    ArrayExpressi Q9Y3C7.
    Bgeei Q9Y3C7.
    CleanExi HS_MED31.
    Genevestigatori Q9Y3C7.

    Family and domain databases

    InterProi IPR008831. Mediator_Med31.
    [Graphical view ]
    PANTHERi PTHR13186. PTHR13186. 1 hit.
    Pfami PF05669. Med31. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
      Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
      Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    5. "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."
      Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G.
      Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SMCC COMPLEX.
    6. "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro."
      Kang Y.K., Guermah M., Yuan C.-X., Roeder R.G.
      Proc. Natl. Acad. Sci. U.S.A. 99:2642-2647(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH ESR1 AND ESR2.
    7. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
      Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED19.
    8. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    9. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    10. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMED31_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y3C7
    Secondary accession number(s): B2R4L9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2003
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3