Q9Y3C0 (CCD53_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WASH complex subunit CCDC53

Alternative name(s):
Coiled-coil domain-containing protein 53

Gene names

Name:	CCDC53
ORF Names:	AD-016, CGI-116, x0009

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Ref.8
Subunit structure	Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Ref.8
Sequence similarities	Belongs to the CCDC53 family.

Ontologies

Keywords
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	WASH complex Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MCRS1	Q96EZ8	3	EBI-712969,EBI-348259
PBXIP1	Q96AQ6	3	EBI-712969,EBI-740845

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	WASH complex subunit CCDC53		PRO_0000076201
Regions
	Coiled coil	46 – 74	29	Potential
Experimental info
	Sequence conflict	81	1	D → G in BAD97059. Ref.5
	Sequence conflict	110	1	N → S in AAH10889. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9Y3C0 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: B0CCF1AE76CDA6D2	FASTA	194	21,173
	10 20 30 40 50 60 MDEDGLPLMG SGIDLTKVPA IQQKRTVAFL NQFVVHTVQF LNRFSTVCEE KLADLSLRIQ 70 80 90 100 110 120 QIETTLNILD AKLSSIPGLD DVTVEVSPLN VTSVTNGAHP EATSEQPQQN STQDSGLQES 130 140 150 160 170 180 EVSAENILTV AKDPRYARYL KMVQVGVPVM AIRNKMISEG LDPDLLERPD APVPDGESEK 190 TVEESSDSES SFSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland.
[2]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[8]	"The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex." Derivery E., Sousa C., Gautier J.J., Lombard B., Loew D., Gautreau A. Dev. Cell 17:712-723(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE WASH COMPLEX, IDENTIFICATION IN THE WASH COMPLEX.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF155655 mRNA. Translation: AAF67012.1. AF151874 mRNA. Translation: AAD34111.1. CR457171 mRNA. Translation: CAG33452.1. AK223339 mRNA. Translation: BAD97059.1. AK314970 mRNA. Translation: BAG37471.1. CH471054 Genomic DNA. Translation: EAW97687.1. BC010889 mRNA. Translation: AAH10889.1.
IPI	IPI00032848.
RefSeq	NP_057137.1. NM_016053.2.
UniGene	Hs.405692.
3D structure databases
ProteinModelPortal	Q9Y3C0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y3C0. 19 interactions.
MINT	MINT-1369211.
STRING	9606.ENSP00000240079.
PTM databases
PhosphoSite	Q9Y3C0.
Polymorphism databases
DMDM	6831732.
Proteomic databases
PaxDb	Q9Y3C0.
PRIDE	Q9Y3C0.
Protocols and materials databases
DNASU	51019.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000240079; ENSP00000240079; ENSG00000120860.
GeneID	51019.
KEGG	hsa:51019.
UCSC	uc010svw.2. human.
Organism-specific databases
CTD	51019.
GeneCards	GC12M102406.
HGNC	HGNC:24256. CCDC53.
HPA	HPA038338.
neXtProt	NX_Q9Y3C0.
PharmGKB	PA142672169.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG261899.
HOGENOM	HOG000006526.
HOVERGEN	HBG009789.
InParanoid	Q9Y3C0.
OMA	TSEQSQQ.
OrthoDB	EOG48GW4G.
Gene expression databases
ArrayExpress	Q9Y3C0.
Bgee	Q9Y3C0.
CleanEx	HS_CCDC53.
Genevestigator	Q9Y3C0.
GermOnline	ENSG00000120860. Homo sapiens.
Family and domain databases
InterPro	IPR019309. WASH_CCDC53. [Graphical view]
Pfam	PF10152. DUF2360. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CCDC53. human.
GenomeRNAi	51019.
NextBio	53536.

Entry information

Entry name

CCD53_HUMAN

Accession

Primary (citable) accession number: Q9Y3C0
Secondary accession number(s): B2RC74 , Q53FF0, Q6IAI4, Q96QK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.