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Q9Y3C0 (CCD53_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WASH complex subunit CCDC53
Alternative name(s):
Coiled-coil domain-containing protein 53
Gene names
Name:CCDC53
ORF Names:AD-016, CGI-116, x0009
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length194 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Ref.8

Subunit structure

Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Ref.8

Sequence similarities

Belongs to the CCDC53 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MCRS1Q96EZ83EBI-712969,EBI-348259
PBXIP1Q96AQ63EBI-712969,EBI-740845

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 194194WASH complex subunit CCDC53
PRO_0000076201

Regions

Coiled coil46 – 7429 Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10

Experimental info

Sequence conflict811D → G in BAD97059. Ref.5
Sequence conflict1101N → S in AAH10889. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9Y3C0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: B0CCF1AE76CDA6D2

FASTA19421,173
        10         20         30         40         50         60 
MDEDGLPLMG SGIDLTKVPA IQQKRTVAFL NQFVVHTVQF LNRFSTVCEE KLADLSLRIQ 

        70         80         90        100        110        120 
QIETTLNILD AKLSSIPGLD DVTVEVSPLN VTSVTNGAHP EATSEQPQQN STQDSGLQES 

       130        140        150        160        170        180 
EVSAENILTV AKDPRYARYL KMVQVGVPVM AIRNKMISEG LDPDLLERPD APVPDGESEK 

       190 
TVEESSDSES SFSD 

« Hide

References

« Hide 'large scale' references
[1]"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. expand/collapse author list , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Adrenal gland.
[2]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[5]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[8]"The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex."
Derivery E., Sousa C., Gautier J.J., Lombard B., Loew D., Gautreau A.
Dev. Cell 17:712-723(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE WASH COMPLEX, IDENTIFICATION IN THE WASH COMPLEX.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF155655 mRNA. Translation: AAF67012.1.
AF151874 mRNA. Translation: AAD34111.1.
CR457171 mRNA. Translation: CAG33452.1.
AK223339 mRNA. Translation: BAD97059.1.
AK314970 mRNA. Translation: BAG37471.1.
CH471054 Genomic DNA. Translation: EAW97687.1.
BC010889 mRNA. Translation: AAH10889.1.
CCDSCCDS44959.1.
RefSeqNP_057137.1. NM_016053.2.
UniGeneHs.405692.

3D structure databases

ProteinModelPortalQ9Y3C0.
SMRQ9Y3C0. Positions 15-67.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119225. 21 interactions.
IntActQ9Y3C0. 20 interactions.
MINTMINT-1369211.
STRING9606.ENSP00000240079.

PTM databases

PhosphoSiteQ9Y3C0.

Polymorphism databases

DMDM6831732.

Proteomic databases

MaxQBQ9Y3C0.
PaxDbQ9Y3C0.
PRIDEQ9Y3C0.

Protocols and materials databases

DNASU51019.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000240079; ENSP00000240079; ENSG00000120860.
GeneID51019.
KEGGhsa:51019.
UCSCuc010svw.2. human.

Organism-specific databases

CTD51019.
GeneCardsGC12M102406.
HGNCHGNC:24256. CCDC53.
HPAHPA038338.
HPA038339.
neXtProtNX_Q9Y3C0.
PharmGKBPA142672169.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG261899.
HOGENOMHOG000006526.
HOVERGENHBG009789.
InParanoidQ9Y3C0.
OMAHTVRFLN.
PhylomeDBQ9Y3C0.
TreeFamTF318955.

Gene expression databases

ArrayExpressQ9Y3C0.
BgeeQ9Y3C0.
CleanExHS_CCDC53.
GenevestigatorQ9Y3C0.

Family and domain databases

InterProIPR019309. WASH_CCDC53.
[Graphical view]
PfamPF10152. DUF2360. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCCDC53. human.
GeneWikiCCDC53.
GenomeRNAi51019.
NextBio53536.
PROQ9Y3C0.

Entry information

Entry nameCCD53_HUMAN
AccessionPrimary (citable) accession number: Q9Y3C0
Secondary accession number(s): B2RC74 expand/collapse secondary AC list , Q53FF0, Q6IAI4, Q96QK0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM