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Q9Y3B8 (ORN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oligoribonuclease, mitochondrial

EC=3.1.-.-
Alternative name(s):
RNA exonuclease 2 homolog
Small fragment nuclease
Gene names
Name:REXO2
Synonyms:SFN, SMFN
ORF Names:CGI-114
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

3'-to-5' exoribonuclease specific for small oligoribonucleotides. Active on small (primarily </=5 nucleotides in length) single-stranded RNA and DNA oligomers. May have a role in cellular nucleotide recycling. HAMAP-Rule MF_00045

Cofactor

Manganese.

Enzyme regulation

Inhibited by adenosine 3',5'-bisphosphate. Ref.8

Subunit structure

Homotetramer Probable.

Subcellular location

Isoform 1: Mitochondrion HAMAP-Rule MF_00045.

Isoform 2: Nucleus HAMAP-Rule MF_00045.

Sequence similarities

Belongs to the oligoribonuclease family.

Contains 1 exonuclease domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y3B8-1)

Also known as: Sfn-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y3B8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2525Mitochondrion Potential
Chain26 – 237212Oligoribonuclease, mitochondrial HAMAP-Rule MF_00045
PRO_0000020273

Regions

Domain43 – 207165Exonuclease

Sites

Active site1641 Potential

Amino acid modifications

Modified residue1221Phosphotyrosine Ref.7
Modified residue1731N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 3838Missing in isoform 2.
VSP_003775

Experimental info

Mutagenesis1681D → A: Abolishes activity.
Sequence conflict1031K → R in CAB53690. Ref.2
Sequence conflict1031K → R in CAG38531. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Sfn-alpha) [UniParc].

Last modified October 17, 2006. Version 3.
Checksum: A0343A8918B11B82

FASTA23726,833
        10         20         30         40         50         60 
MLGGSLGSRL LRGVGGSHGR FGARGVREGG AAMAAGESMA QRMVWVDLEM TGLDIEKDQI 

        70         80         90        100        110        120 
IEMACLITDS DLNILAEGPN LIIKQPDELL DSMSDWCKEH HGKSGLTKAV KESTITLQQA 

       130        140        150        160        170        180 
EYEFLSFVRQ QTPPGLCPLA GNSVHEDKKF LDKYMPQFMK HLHYRIIDVS TVKELCRRWY 

       190        200        210        220        230 
PEEYEFAPKK AASHRALDDI SESIKELQFY RNNIFKKKID EKKRKIIENG ENEKTVS 

« Hide

Isoform 2 [UniParc].

Checksum: E96469443EFF140A
Show »

FASTA19923,208

References

« Hide 'large scale' references
[1]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney.
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney proximal tubule.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Placenta.
[6]"The human homolog of Escherichia coli Orn degrades small single-stranded RNA and DNA oligomers."
Nguyen L.H., Erzberger J.P., Root J., Wilson D.M. III
J. Biol. Chem. 275:25900-25906(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Oligoribonuclease is a common downstream target of lithium-induced pAp accumulation in Escherichia coli and human cells."
Mechold U., Ogryzko V., Ngo S., Danchin A.
Nucleic Acids Res. 34:2364-2373(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF151872 mRNA. Translation: AAD34109.1.
AL110239 mRNA. Translation: CAB53690.1.
CR533500 mRNA. Translation: CAG38531.1.
AK223200 mRNA. Translation: BAD96920.1.
BC105024 mRNA. Translation: AAI05025.1.
BC105026 mRNA. Translation: AAI05027.1.
BC107887 mRNA. Translation: AAI07888.1.
PIRT14770.
RefSeqNP_056338.2. NM_015523.3.
UniGeneHs.677190.
Hs.714231.

3D structure databases

ProteinModelPortalQ9Y3B8.
SMRQ9Y3B8. Positions 41-216.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117473. 2 interactions.
MINTMINT-3085757.
STRING9606.ENSP00000265881.

PTM databases

PhosphoSiteQ9Y3B8.

Polymorphism databases

DMDM116242694.

Proteomic databases

PaxDbQ9Y3B8.
PRIDEQ9Y3B8.

Protocols and materials databases

DNASU25996.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265881; ENSP00000265881; ENSG00000076043. [Q9Y3B8-1]
GeneID25996.
KEGGhsa:25996.
UCSCuc001poy.3. human. [Q9Y3B8-1]

Organism-specific databases

CTD25996.
GeneCardsGC11P114311.
H-InvDBHIX0010145.
HGNCHGNC:17851. REXO2.
HPAHPA038450.
HPA038451.
MIM607149. gene.
neXtProtNX_Q9Y3B8.
PharmGKBPA142671085.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1949.
HOGENOMHOG000246596.
HOVERGENHBG002323.
InParanoidQ9Y3B8.
KOK13288.
OMAAFFHYRN.
OrthoDBEOG7J4483.
PhylomeDBQ9Y3B8.
TreeFamTF314084.

Gene expression databases

ArrayExpressQ9Y3B8.
BgeeQ9Y3B8.
CleanExHS_REXO2.
HS_SFN.
GenevestigatorQ9Y3B8.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00045. Oligoribonuclease.
InterProIPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR022894. Oligoribonuclease.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 1 hit.
ProtoNetSearch...

Other

ChiTaRSREXO2. human.
GeneWikiREXO2.
GenomeRNAi25996.
NextBio47710.
PROQ9Y3B8.
SOURCESearch...

Entry information

Entry nameORN_HUMAN
AccessionPrimary (citable) accession number: Q9Y3B8
Secondary accession number(s): Q32Q18 expand/collapse secondary AC list , Q53FT1, Q6FIC6, Q9UFY7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM