Q9Y3B8 (ORN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligoribonuclease, mitochondrial
EC=3.1.-.-

Alternative name(s):
RNA exonuclease 2 homolog
Small fragment nuclease

Gene names

Name:	REXO2
Synonyms:	SFN, SMFN
ORF Names:	CGI-114

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	3'-to-5' exoribonuclease specific for small oligoribonucleotides. Active on small (primarily </=5 nucleotides in length) single-stranded RNA and DNA oligomers. May have a role in cellular nucleotide recycling.
Cofactor	Manganese.
Enzyme regulation	Inhibited by adenosine 3',5'-bisphosphate. Ref.8
Subunit structure	Homotetramer Probable.
Subcellular location	Isoform 1: Mitochondrion. Isoform 2: Nucleus.
Sequence similarities	Belongs to the oligoribonuclease family. Contains 1 exonuclease domain.

Ontologies

Keywords
Cellular component	Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Manganese
Molecular function	Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleotide metabolic process Traceable author statement Ref.6. Source: ProtInc
Cellular_component	mitochondrion Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	3'-5' exonuclease activity Inferred from direct assay Ref.6. Source: MGI nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y3B8-1)

Also known as: Sfn-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y3B8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion Potential
	Chain	26 – 237	212	Oligoribonuclease, mitochondrial		PRO_0000020273
Regions
	Domain	43 – 207	165	Exonuclease
Sites
	Active site	164	1	Potential
Amino acid modifications
	Modified residue	122	1	Phosphotyrosine Ref.7
	Modified residue	184	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	1 – 38	38	Missing in isoform 2.		VSP_003775
Experimental info
	Mutagenesis	168	1	D → A: Abolishes activity.
	Sequence conflict	103	1	K → R in CAB53690. Ref.2
	Sequence conflict	103	1	K → R in CAG38531. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Sfn-alpha) [UniParc]. Last modified October 17, 2006. Version 3. Checksum: A0343A8918B11B82	FASTA	237	26,833
	10 20 30 40 50 60 MLGGSLGSRL LRGVGGSHGR FGARGVREGG AAMAAGESMA QRMVWVDLEM TGLDIEKDQI 70 80 90 100 110 120 IEMACLITDS DLNILAEGPN LIIKQPDELL DSMSDWCKEH HGKSGLTKAV KESTITLQQA 130 140 150 160 170 180 EYEFLSFVRQ QTPPGLCPLA GNSVHEDKKF LDKYMPQFMK HLHYRIIDVS TVKELCRRWY 190 200 210 220 230 PEEYEFAPKK AASHRALDDI SESIKELQFY RNNIFKKKID EKKRKIIENG ENEKTVS
	Isoform 2 [UniParc]. Checksum: E96469443EFF140A	FASTA	199	23,208
	10 20 30 40 50 60 MAQRMVWVDL EMTGLDIEKD QIIEMACLIT DSDLNILAEG PNLIIKQPDE LLDSMSDWCK 70 80 90 100 110 120 EHHGKSGLTK AVKESTITLQ QAEYEFLSFV RQQTPPGLCP LAGNSVHEDK KFLDKYMPQF 130 140 150 160 170 180 MKHLHYRIID VSTVKELCRR WYPEEYEFAP KKAASHRALD DISESIKELQ FYRNNIFKKK 190 IDEKKRKIIE NGENEKTVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney proximal tubule.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Placenta.
[6]	"The human homolog of Escherichia coli Orn degrades small single-stranded RNA and DNA oligomers." Nguyen L.H., Erzberger J.P., Root J., Wilson D.M. III J. Biol. Chem. 275:25900-25906(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-122, MASS SPECTROMETRY.
[8]	"Oligoribonuclease is a common downstream target of lithium-induced pAp accumulation in Escherichia coli and human cells." Mechold U., Ogryzko V., Ngo S., Danchin A. Nucleic Acids Res. 34:2364-2373(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF151872 mRNA. Translation: AAD34109.1. AL110239 mRNA. Translation: CAB53690.1. CR533500 mRNA. Translation: CAG38531.1. AK223200 mRNA. Translation: BAD96920.1. BC105024 mRNA. Translation: AAI05025.1. BC105026 mRNA. Translation: AAI05027.1. BC107887 mRNA. Translation: AAI07888.1.
IPI	IPI00032830. IPI00217330.
PIR	T14770.
RefSeq	NP_056338.2. NM_015523.3.
UniGene	Hs.677190. Hs.714231.
3D structure databases
ProteinModelPortal	Q9Y3B8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000265881.
PTM databases
PhosphoSite	Q9Y3B8.
Polymorphism databases
DMDM	116242694.
Proteomic databases
PaxDb	Q9Y3B8.
PRIDE	Q9Y3B8.
Protocols and materials databases
DNASU	25996.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265881; ENSP00000265881; ENSG00000076043.
GeneID	25996.
KEGG	hsa:25996.
UCSC	uc001poy.3. human.
Organism-specific databases
CTD	25996.
GeneCards	GC11P114311.
H-InvDB	HIX0010145.
HGNC	HGNC:17851. REXO2.
HPA	HPA038450. HPA038451.
MIM	607149. gene.
neXtProt	NX_Q9Y3B8.
PharmGKB	PA142671085.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1949.
HOGENOM	HOG000246596.
HOVERGEN	HBG002323.
InParanoid	Q9Y3B8.
KO	K13288.
OMA	LEAFFHY.
OrthoDB	EOG437RG0.
PhylomeDB	Q9Y3B8.
Gene expression databases
ArrayExpress	Q9Y3B8.
Bgee	Q9Y3B8.
CleanEx	HS_REXO2. HS_SFN.
Genevestigator	Q9Y3B8.
GermOnline	ENSG00000076043. Homo sapiens.
Family and domain databases
InterPro	IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR022894. Oligoribonuclease. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF00929. RNase_T. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	REXO2. human.
GenomeRNAi	25996.
NextBio	47710.
SOURCE	Search...

Entry information

Entry name

ORN_HUMAN

Accession

Primary (citable) accession number: Q9Y3B8
Secondary accession number(s): Q32Q18 , Q53FT1, Q6FIC6, Q9UFY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.