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Protein

Transmembrane emp24 domain-containing protein 7

Gene

TMED7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Appears to play a role in the biosynthesis of secreted cargo including processing and post-translational modifications.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 7
Alternative name(s):
p24 family protein gamma-3
Short name:
p24gamma3
p27
Gene namesi
Name:TMED7
ORF Names:CGI-109
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:24253. TMED7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini35 – 187153LumenalSequence analysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence analysisAdd
BLAST
Topological domaini209 – 22416CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • COPII vesicle coat Source: UniProtKB
  • COPI vesicle coat Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment membrane Source: Reactome
  • endoplasmic reticulum membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • transport vesicle Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134891536.
PA165660570.

Polymorphism and mutation databases

BioMutaiTMED7.
DMDMi62299080.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Sequence analysisAdd
BLAST
Chaini35 – 224190Transmembrane emp24 domain-containing protein 7PRO_0000010395Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-linked glycosylated in complex form containing terminal sialic acid.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9Y3B3.
MaxQBiQ9Y3B3.
PaxDbiQ9Y3B3.
PRIDEiQ9Y3B3.
TopDownProteomicsiQ9Y3B3-1. [Q9Y3B3-1]

PTM databases

iPTMnetiQ9Y3B3.
PhosphoSiteiQ9Y3B3.
SwissPalmiQ9Y3B3.

Expressioni

Gene expression databases

BgeeiQ9Y3B3.
CleanExiHS_TMED7.
ExpressionAtlasiQ9Y3B3. baseline and differential.
GenevisibleiQ9Y3B3. HS.

Organism-specific databases

HPAiCAB025883.
HPA008960.

Interactioni

Subunit structurei

Predominantly monomeric and to lesser extent homodimeric in endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED9 and TMED10. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED7.3 Publications

Protein-protein interaction databases

BioGridi119221. 15 interactions.
IntActiQ9Y3B3. 11 interactions.
STRINGi9606.ENSP00000405926.

Structurei

3D structure databases

ProteinModelPortaliQ9Y3B3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 12883GOLDPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi211 – 22414COPI vesicle coat-bindingSequence analysisAdd
BLAST
Motifi211 – 2122COPII vesicle coat-bindingSequence analysis

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ9Y3B3.
KOiK14825.
OMAiIMVLRNF.
OrthoDBiEOG70ZZP7.
PhylomeDBiQ9Y3B3.
TreeFamiTF313000.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015718. TMED7.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PTHR22811:SF49. PTHR22811:SF49. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y3B3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRPGSAQRW AAVAGRWGCR LLALLLLVPG PGGASEITFE LPDNAKQCFY
60 70 80 90 100
EDIAQGTKCT LEFQVITGGH YDVDCRLEDP DGKVLYKEMK KQYDSFTFTA
110 120 130 140 150
SKNGTYKFCF SNEFSTFTHK TVYFDFQVGE DPPLFPSENR VSALTQMESA
160 170 180 190 200
CVSIHEALKS VIDYQTHFRL REAQGRSRAE DLNTRVAYWS VGEALILLVV
210 220
SIGQVFLLKS FFSDKRTTTT RVGS
Length:224
Mass (Da):25,172
Last modified:March 29, 2005 - v2
Checksum:i9D0D2110579837B3
GO
Isoform 2 (identifier: Q9Y3B3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-224: Missing.

Note: No experimental confirmation available.
Show »
Length:188
Mass (Da):21,233
Checksum:i15A10CC4BD553DB3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 2323MPRPG…CRLLA → MGSTVPRSASVLLLL in AAD34104 (PubMed:10810093).CuratedAdd
BLAST
Sequence conflicti27 – 3711LVPGPGGASEI → RRAEQPCGAEL in AAD34104 (PubMed:10810093).CuratedAdd
BLAST
Sequence conflicti59 – 591C → S in AAD34104 (PubMed:10810093).Curated
Sequence conflicti131 – 1399DPPLFPSEN → THLCFLVD in AAD34104 (PubMed:10810093).Curated
Sequence conflicti213 – 2131S → P in BAB55166 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei189 – 22436Missing in isoform 2. 1 PublicationVSP_046247Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151867 mRNA. Translation: AAD34104.1.
AK027512 mRNA. Translation: BAB55166.1.
AK074962 mRNA. Translation: BAC11318.1.
AK075218 mRNA. Translation: BAC11479.1.
AC010226 Genomic DNA. No translation available.
BC019349 mRNA. Translation: AAH19349.1.
CCDSiCCDS4120.1. [Q9Y3B3-1]
RefSeqiNP_001157941.1. NM_001164469.3. [Q9Y3B3-2]
NP_861974.1. NM_181836.5. [Q9Y3B3-1]
UniGeneiHs.642817.
Hs.718838.

Genome annotation databases

EnsembliENST00000456936; ENSP00000405926; ENSG00000134970. [Q9Y3B3-1]
GeneIDi100302736.
51014.
KEGGihsa:51014.
UCSCiuc003krf.4. human. [Q9Y3B3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151867 mRNA. Translation: AAD34104.1.
AK027512 mRNA. Translation: BAB55166.1.
AK074962 mRNA. Translation: BAC11318.1.
AK075218 mRNA. Translation: BAC11479.1.
AC010226 Genomic DNA. No translation available.
BC019349 mRNA. Translation: AAH19349.1.
CCDSiCCDS4120.1. [Q9Y3B3-1]
RefSeqiNP_001157941.1. NM_001164469.3. [Q9Y3B3-2]
NP_861974.1. NM_181836.5. [Q9Y3B3-1]
UniGeneiHs.642817.
Hs.718838.

3D structure databases

ProteinModelPortaliQ9Y3B3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119221. 15 interactions.
IntActiQ9Y3B3. 11 interactions.
STRINGi9606.ENSP00000405926.

PTM databases

iPTMnetiQ9Y3B3.
PhosphoSiteiQ9Y3B3.
SwissPalmiQ9Y3B3.

Polymorphism and mutation databases

BioMutaiTMED7.
DMDMi62299080.

Proteomic databases

EPDiQ9Y3B3.
MaxQBiQ9Y3B3.
PaxDbiQ9Y3B3.
PRIDEiQ9Y3B3.
TopDownProteomicsiQ9Y3B3-1. [Q9Y3B3-1]

Protocols and materials databases

DNASUi51014.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000456936; ENSP00000405926; ENSG00000134970. [Q9Y3B3-1]
GeneIDi100302736.
51014.
KEGGihsa:51014.
UCSCiuc003krf.4. human. [Q9Y3B3-1]

Organism-specific databases

CTDi100302736.
51014.
GeneCardsiTMED7.
HGNCiHGNC:24253. TMED7.
HPAiCAB025883.
HPA008960.
neXtProtiNX_Q9Y3B3.
PharmGKBiPA134891536.
PA165660570.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ9Y3B3.
KOiK14825.
OMAiIMVLRNF.
OrthoDBiEOG70ZZP7.
PhylomeDBiQ9Y3B3.
TreeFamiTF313000.

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiQ9Y3B3.

Gene expression databases

BgeeiQ9Y3B3.
CleanExiHS_TMED7.
ExpressionAtlasiQ9Y3B3. baseline and differential.
GenevisibleiQ9Y3B3. HS.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015718. TMED7.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PTHR22811:SF49. PTHR22811:SF49. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Placenta.
  4. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  6. "Localization and recycling of gp27 (hp24gamma3): complex formation with other p24 family members."
    Fullekrug J., Suganuma T., Tang B.L., Hong W., Storrie B., Nilsson T.
    Mol. Biol. Cell 10:1939-1955(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, SUBUNIT.
  7. "Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway."
    Jenne N., Frey K., Brugger B., Wieland F.T.
    J. Biol. Chem. 277:46504-46511(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT.
  8. "Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins."
    Bethune J., Kol M., Hoffmann J., Reckmann I., Brugger B., Wieland F.
    Mol. Cell. Biol. 26:8011-8021(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH COPG1.

Entry informationi

Entry nameiTMED7_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3B3
Secondary accession number(s): Q8NBU8, Q8WUU6, Q96K51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: March 29, 2005
Last modified: June 8, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.