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Protein

MOB-like protein phocein

Gene

MOB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in membrane trafficking, specifically in membrane budding reactions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921ZincBy similarity
Metal bindingi97 – 971ZincBy similarity
Metal bindingi169 – 1691ZincBy similarity
Metal bindingi174 – 1741ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
MOB-like protein phocein
Alternative name(s):
2C4D
Class II mMOB1
Mob1 homolog 3
Short name:
Mob3
Mps one binder kinase activator-like 3
Preimplantation protein 3
Gene namesi
Name:MOB4
Synonyms:MOB3, MOBKL3, PHOCN, PREI3
ORF Names:CGI-95
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:17261. MOB4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162396053.

Polymorphism and mutation databases

BioMutaiMOB4.
DMDMi56749365.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225MOB-like protein phoceinPRO_0000193576Add
BLAST

Post-translational modificationi

Phosphorylated on serine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Y3A3.
PaxDbiQ9Y3A3.
PRIDEiQ9Y3A3.

2D gel databases

OGPiQ9Y3A3.

PTM databases

PhosphoSiteiQ9Y3A3.

Expressioni

Gene expression databases

BgeeiQ9Y3A3.
CleanExiHS_MOBKL3.
ExpressionAtlasiQ9Y3A3. baseline and differential.
GenevestigatoriQ9Y3A3.

Organism-specific databases

HPAiCAB034082.
HPA044125.

Interactioni

Subunit structurei

Binds STRN4 (By similarity). Interacts with DNM1 and EPS15 (By similarity). Interacts with nucleoside diphosphate kinase (By similarity). Binds STRN and STRN3. Part of a ternary complex containing MOB4/PHOCN, STRN and/or STRN3 and PPA2. Interacts with CTTNBP2 (By similarity). Interacts with CTTNBP2NL.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTTNBP2NLQ9P2B46EBI-713935,EBI-1774273
STRNO438154EBI-713935,EBI-1046642
TRAF3IP3Q9Y2282EBI-713935,EBI-765817

Protein-protein interaction databases

BioGridi117369. 64 interactions.
IntActiQ9Y3A3. 62 interactions.
MINTiMINT-1380326.
STRINGi9606.ENSP00000315702.

Structurei

3D structure databases

ProteinModelPortaliQ9Y3A3.
SMRiQ9Y3A3. Positions 61-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MOB1/phocein family.Curated

Phylogenomic databases

eggNOGiNOG297181.
GeneTreeiENSGT00390000012143.
HOVERGENiHBG052490.
InParanoidiQ9Y3A3.
OrthoDBiEOG7VDXQ5.
PhylomeDBiQ9Y3A3.
TreeFamiTF314078.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. Mob1_phocein.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y3A3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMAEGTAVL RRNRPGTKAQ DFYNWPDESF DEMDSTLAVQ QYIQQNIRAD
60 70 80 90 100
CSNIDKILEP PEGQDEGVWK YEHLRQFCLE LNGLAVKLQS ECHPDTCTQM
110 120 130 140 150
TATEQWIFLC AAHKTPKECP AIDYTRHTLD GAACLLNSNK YFPSRVSIKE
160 170 180 190 200
SSVAKLGSVC RRIYRIFSHA YFHHRQIFDE YENETFLCHR FTKFVMKYNL
210 220
MSKDNLIVPI LEEEVQNSVS GESEA
Length:225
Mass (Da):26,032
Last modified:November 1, 1999 - v1
Checksum:iD0EF1219711458BA
GO
Isoform 2 (identifier: Q9Y3A3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: No experimental confirmation available.

Show »
Length:193
Mass (Da):22,318
Checksum:i37E70D59A5437A7A
GO
Isoform 3 (identifier: Q9Y3A3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-40: Missing.

Show »
Length:204
Mass (Da):23,513
Checksum:i52D1517EA742A30C
GO

Sequence cautioni

The sequence AAP97221.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB45697.1 differs from that shown. Reason: Frameshift at position 168. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901S → G in BAB15635 (PubMed:14702039).Curated
Sequence conflicti135 – 1351L → P in CAB45697 (PubMed:11230166).Curated
Sequence conflicti172 – 1721F → S in BAB15635 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3232Missing in isoform 2. 1 PublicationVSP_012303Add
BLAST
Alternative sequencei20 – 4021Missing in isoform 3. 1 PublicationVSP_041091Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015441 mRNA. Translation: BAB19057.1.
AF093825 mRNA. Translation: AAP97221.1. Different initiation.
AJ580638 mRNA. Translation: CAE45270.1.
AF151853 mRNA. Translation: AAD34090.1.
AF250319 mRNA. Translation: AAG44567.1.
AL080070 mRNA. Translation: CAB45697.1. Frameshift.
AK027043 mRNA. Translation: BAB15635.1.
AK292938 mRNA. Translation: BAF85627.1.
AK297514 mRNA. Translation: BAG59922.1.
CR457371 mRNA. Translation: CAG33652.1.
AC020550 Genomic DNA. Translation: AAX93147.1.
CH471063 Genomic DNA. Translation: EAW70164.1.
CH471063 Genomic DNA. Translation: EAW70167.1.
BC005237 mRNA. Translation: AAH05237.1.
CCDSiCCDS2321.1. [Q9Y3A3-1]
CCDS2322.1. [Q9Y3A3-2]
CCDS46480.1. [Q9Y3A3-3]
PIRiT12466.
RefSeqiNP_001094289.1. NM_001100819.2. [Q9Y3A3-3]
NP_001191023.1. NM_001204094.1. [Q9Y3A3-2]
NP_056202.2. NM_015387.4. [Q9Y3A3-1]
NP_955776.1. NM_199482.3. [Q9Y3A3-2]
UniGeneiHs.633165.

Genome annotation databases

EnsembliENST00000233892; ENSP00000233892; ENSG00000115540. [Q9Y3A3-2]
ENST00000323303; ENSP00000315702; ENSG00000115540. [Q9Y3A3-1]
ENST00000409360; ENSP00000387289; ENSG00000115540. [Q9Y3A3-2]
ENST00000448447; ENSP00000405354; ENSG00000115540. [Q9Y3A3-3]
GeneIDi25843.
KEGGihsa:25843.
UCSCiuc002uum.4. human. [Q9Y3A3-1]
uc010fsn.3. human. [Q9Y3A3-3]
uc021vum.1. human. [Q9Y3A3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015441 mRNA. Translation: BAB19057.1.
AF093825 mRNA. Translation: AAP97221.1. Different initiation.
AJ580638 mRNA. Translation: CAE45270.1.
AF151853 mRNA. Translation: AAD34090.1.
AF250319 mRNA. Translation: AAG44567.1.
AL080070 mRNA. Translation: CAB45697.1. Frameshift.
AK027043 mRNA. Translation: BAB15635.1.
AK292938 mRNA. Translation: BAF85627.1.
AK297514 mRNA. Translation: BAG59922.1.
CR457371 mRNA. Translation: CAG33652.1.
AC020550 Genomic DNA. Translation: AAX93147.1.
CH471063 Genomic DNA. Translation: EAW70164.1.
CH471063 Genomic DNA. Translation: EAW70167.1.
BC005237 mRNA. Translation: AAH05237.1.
CCDSiCCDS2321.1. [Q9Y3A3-1]
CCDS2322.1. [Q9Y3A3-2]
CCDS46480.1. [Q9Y3A3-3]
PIRiT12466.
RefSeqiNP_001094289.1. NM_001100819.2. [Q9Y3A3-3]
NP_001191023.1. NM_001204094.1. [Q9Y3A3-2]
NP_056202.2. NM_015387.4. [Q9Y3A3-1]
NP_955776.1. NM_199482.3. [Q9Y3A3-2]
UniGeneiHs.633165.

3D structure databases

ProteinModelPortaliQ9Y3A3.
SMRiQ9Y3A3. Positions 61-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117369. 64 interactions.
IntActiQ9Y3A3. 62 interactions.
MINTiMINT-1380326.
STRINGi9606.ENSP00000315702.

PTM databases

PhosphoSiteiQ9Y3A3.

Polymorphism and mutation databases

BioMutaiMOB4.
DMDMi56749365.

2D gel databases

OGPiQ9Y3A3.

Proteomic databases

MaxQBiQ9Y3A3.
PaxDbiQ9Y3A3.
PRIDEiQ9Y3A3.

Protocols and materials databases

DNASUi25843.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000233892; ENSP00000233892; ENSG00000115540. [Q9Y3A3-2]
ENST00000323303; ENSP00000315702; ENSG00000115540. [Q9Y3A3-1]
ENST00000409360; ENSP00000387289; ENSG00000115540. [Q9Y3A3-2]
ENST00000448447; ENSP00000405354; ENSG00000115540. [Q9Y3A3-3]
GeneIDi25843.
KEGGihsa:25843.
UCSCiuc002uum.4. human. [Q9Y3A3-1]
uc010fsn.3. human. [Q9Y3A3-3]
uc021vum.1. human. [Q9Y3A3-2]

Organism-specific databases

CTDi25843.
GeneCardsiGC02P198383.
HGNCiHGNC:17261. MOB4.
HPAiCAB034082.
HPA044125.
MIMi609361. gene.
neXtProtiNX_Q9Y3A3.
PharmGKBiPA162396053.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG297181.
GeneTreeiENSGT00390000012143.
HOVERGENiHBG052490.
InParanoidiQ9Y3A3.
OrthoDBiEOG7VDXQ5.
PhylomeDBiQ9Y3A3.
TreeFamiTF314078.

Miscellaneous databases

GeneWikiiMOBKL3.
GenomeRNAii25843.
NextBioi47167.
PROiQ9Y3A3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y3A3.
CleanExiHS_MOBKL3.
ExpressionAtlasiQ9Y3A3. baseline and differential.
GenevestigatoriQ9Y3A3.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. Mob1_phocein.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human MOB3."
    Kagaya S., Todokoro K., Kotani S.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning of a novel human cDNA homology to murine B6D2F1 clone 2C4D mRNA."
    Hu P.R., Yu L., Zhao Y., Yue P., Li M.Z., Zhao S.Y.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Characterization of the human Mob-1 like proteins."
    Florindo C.S., Tavares A.A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Adrenal gland.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  7. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Brain and Trachea.
  8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  9. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  10. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  11. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Urinary bladder.
  12. "A mammalian homolog of yeast MOB1 is both a member and a putative substrate of striatin family-protein phosphatase 2A complexes."
    Moreno C.S., Lane W.S., Pallas D.C.
    J. Biol. Chem. 276:24253-24260(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 57-70 AND 166-175, INTERACTION WITH STRN; STRN3 AND THE PPA2 COMPLEX, PHOSPHORYLATION, SUBCELLULAR LOCATION.
  13. "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein."
    Goudreault M., D'Ambrosio L.M., Kean M.J., Mullin M.J., Larsen B.G., Sanchez A., Chaudhry S., Chen G.I., Sicheri F., Nesvizhskii A.I., Aebersold R., Raught B., Gingras A.C.
    Mol. Cell. Proteomics 8:157-171(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CTTNBP2NL.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPHOCN_HUMAN
AccessioniPrimary (citable) accession number: Q9Y3A3
Secondary accession number(s): B4DML0
, Q53SE0, Q7Z4Y6, Q9H2P3, Q9H5J1, Q9Y4T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 1, 1999
Last modified: April 29, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.