Q9Y3A3 (PHOCN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MOB-like protein phocein

Alternative name(s):
2C4D
Class II mMOB1
Mob1 homolog 3
Short name=Mob3
Mps one binder kinase activator-like 3
Preimplantation protein 3

Gene names

Name:	MOB4
Synonyms:	MOB3, MOBKL3, PHOCN, PREI3
ORF Names:	CGI-95

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in membrane trafficking, specifically in membrane budding reactions By similarity.
Subunit structure	Binds STRN4 By similarity. Interacts with DNM1 and EPS15 By similarity. Interacts with nucleoside diphosphate kinase By similarity. Binds STRN and STRN3. Part of a ternary complex containing MOB4/PHOCN, STRN and/or STRN3 and PPA2. Interacts with CTTNBP2 By similarity. Interacts with CTTNBP2NL. Ref.12 Ref.13
Subcellular location	Cytoplasm › perinuclear region. Membrane; Peripheral membrane protein. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein. Note: In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks. Ref.12
Post-translational modification	Phosphorylated on serine residues. Ref.12
Sequence similarities	Belongs to the MOB1/phocein family.
Sequence caution	The sequence AAP97221.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAB45697.1 differs from that shown. Reason: Frameshift at position 168.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 11251078. Source: UniProtKB Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTTNBP2NL	Q9P2B4	5	EBI-713935,EBI-1774273
STRN	O43815	3	EBI-713935,EBI-1046642
TRAF3IP3	Q9Y228	2	EBI-713935,EBI-765817

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y3A3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y3A3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9Y3A3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     20-40: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	MOB-like protein phocein		PRO_0000193576
Sites
	Metal binding	92	1	Zinc By similarity
	Metal binding	97	1	Zinc By similarity
	Metal binding	169	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
Amino acid modifications
	Modified residue	140	1	N6-acetyllysine Ref.14
Natural variations
	Alternative sequence	1 – 32	32	Missing in isoform 2.		VSP_012303
	Alternative sequence	20 – 40	21	Missing in isoform 3.		VSP_041091
Experimental info
	Sequence conflict	90	1	S → G in BAB15635. Ref.7
	Sequence conflict	135	1	L → P in CAB45697. Ref.6
	Sequence conflict	172	1	F → S in BAB15635. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D0EF1219711458BA	FASTA	225	26,032
	10 20 30 40 50 60 MVMAEGTAVL RRNRPGTKAQ DFYNWPDESF DEMDSTLAVQ QYIQQNIRAD CSNIDKILEP 70 80 90 100 110 120 PEGQDEGVWK YEHLRQFCLE LNGLAVKLQS ECHPDTCTQM TATEQWIFLC AAHKTPKECP 130 140 150 160 170 180 AIDYTRHTLD GAACLLNSNK YFPSRVSIKE SSVAKLGSVC RRIYRIFSHA YFHHRQIFDE 190 200 210 220 YENETFLCHR FTKFVMKYNL MSKDNLIVPI LEEEVQNSVS GESEA
	Isoform 2 [UniParc]. Checksum: 37E70D59A5437A7A	FASTA	193	22,318
	10 20 30 40 50 60 MDSTLAVQQY IQQNIRADCS NIDKILEPPE GQDEGVWKYE HLRQFCLELN GLAVKLQSEC 70 80 90 100 110 120 HPDTCTQMTA TEQWIFLCAA HKTPKECPAI DYTRHTLDGA ACLLNSNKYF PSRVSIKESS 130 140 150 160 170 180 VAKLGSVCRR IYRIFSHAYF HHRQIFDEYE NETFLCHRFT KFVMKYNLMS KDNLIVPILE 190 EEVQNSVSGE SEA
	Isoform 3 [UniParc]. Checksum: 52D1517EA742A30C	FASTA	204	23,513
	10 20 30 40 50 60 MVMAEGTAVL RRNRPGTKAQ YIQQNIRADC SNIDKILEPP EGQDEGVWKY EHLRQFCLEL 70 80 90 100 110 120 NGLAVKLQSE CHPDTCTQMT ATEQWIFLCA AHKTPKECPA IDYTRHTLDG AACLLNSNKY 130 140 150 160 170 180 FPSRVSIKES SVAKLGSVCR RIYRIFSHAY FHHRQIFDEY ENETFLCHRF TKFVMKYNLM 190 200 SKDNLIVPIL EEEVQNSVSG ESEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human MOB3." Kagaya S., Todokoro K., Kotani S. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning of a novel human cDNA homology to murine B6D2F1 clone 2C4D mRNA." Hu P.R., Yu L., Zhao Y., Yue P., Li M.Z., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Characterization of the human Mob-1 like proteins." Florindo C.S., Tavares A.A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Adrenal gland.
[6]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain and Trachea.
[8]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[9]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Urinary bladder.
[12]	"A mammalian homolog of yeast MOB1 is both a member and a putative substrate of striatin family-protein phosphatase 2A complexes." Moreno C.S., Lane W.S., Pallas D.C. J. Biol. Chem. 276:24253-24260(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 57-70 AND 166-175, INTERACTION WITH STRN; STRN3 AND THE PPA2 COMPLEX, PHOSPHORYLATION, SUBCELLULAR LOCATION.
[13]	"A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein." Goudreault M., D'Ambrosio L.M., Kean M.J., Mullin M.J., Larsen B.G., Sanchez A., Chaudhry S., Chen G.I., Sicheri F., Nesvizhskii A.I., Aebersold R., Raught B., Gingras A.C. Mol. Cell. Proteomics 8:157-171(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CTTNBP2NL.
[14]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-140, MASS SPECTROMETRY.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015441 mRNA. Translation: BAB19057.1. AF093825 mRNA. Translation: AAP97221.1. Different initiation. AJ580638 mRNA. Translation: CAE45270.1. AF151853 mRNA. Translation: AAD34090.1. AF250319 mRNA. Translation: AAG44567.1. AL080070 mRNA. Translation: CAB45697.1. Frameshift. AK027043 mRNA. Translation: BAB15635.1. AK292938 mRNA. Translation: BAF85627.1. AK297514 mRNA. Translation: BAG59922.1. CR457371 mRNA. Translation: CAG33652.1. AC020550 Genomic DNA. Translation: AAX93147.1. CH471063 Genomic DNA. Translation: EAW70164.1. CH471063 Genomic DNA. Translation: EAW70167.1. BC005237 mRNA. Translation: AAH05237.1.
IPI	IPI00386122. IPI00398774. IPI00856011.
PIR	T12466.
RefSeq	NP_001094289.1. NM_001100819.2. NP_001191023.1. NM_001204094.1. NP_056202.2. NM_015387.4. NP_955776.1. NM_199482.3.
UniGene	Hs.633165.
3D structure databases
ProteinModelPortal	Q9Y3A3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y3A3. 59 interactions.
MINT	MINT-1380326.
STRING	9606.ENSP00000315702.
PTM databases
PhosphoSite	Q9Y3A3.
Polymorphism databases
DMDM	56749365.
2D gel databases
OGP	Q9Y3A3.
Proteomic databases
PaxDb	Q9Y3A3.
PRIDE	Q9Y3A3.
Protocols and materials databases
DNASU	25843.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000233892; ENSP00000233892; ENSG00000115540. ENST00000323303; ENSP00000315702; ENSG00000115540. ENST00000409360; ENSP00000387289; ENSG00000115540. ENST00000448447; ENSP00000405354; ENSG00000115540.
GeneID	25843.
KEGG	hsa:25843.
UCSC	uc002uum.4. human. uc010fsn.3. human. uc021vum.1. human.
Organism-specific databases
CTD	25843.
GeneCards	GC02P198383.
HGNC	HGNC:17261. MOB4.
HPA	CAB034082. HPA044125.
MIM	609361. gene.
neXtProt	NX_Q9Y3A3.
PharmGKB	PA162396053.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG297181.
HOVERGEN	HBG052490.
InParanoid	Q9Y3A3.
OMA	CRWPDEP.
OrthoDB	EOG4Q2DGB.
PhylomeDB	Q9Y3A3.
Gene expression databases
ArrayExpress	Q9Y3A3.
Bgee	Q9Y3A3.
CleanEx	HS_MOBKL3.
Genevestigator	Q9Y3A3.
GermOnline	ENSG00000115540. Homo sapiens.
Family and domain databases
Gene3D	1.20.140.30. 1 hit.
InterPro	IPR005301. Mob1_phocein. [Graphical view]
PANTHER	PTHR22599. PTHR22599. 1 hit.
Pfam	PF03637. Mob1_phocein. 1 hit. [Graphical view]
SUPFAM	SSF101152. Mob1_phocein. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	25843.
NextBio	47167.
SOURCE	Search...

Entry information

Entry name

PHOCN_HUMAN

Accession

Primary (citable) accession number: Q9Y3A3
Secondary accession number(s): B4DML0 , Q53SE0, Q7Z4Y6, Q9H2P3, Q9H5J1, Q9Y4T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.