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Q9Y3A3 (PHOCN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
MOB-like protein phocein
Alternative name(s):
2C4D
Class II mMOB1
Mob1 homolog 3
Short name=Mob3
Mps one binder kinase activator-like 3
Preimplantation protein 3
Gene names
Name:MOB4
Synonyms:MOB3, MOBKL3, PHOCN, PREI3
ORF Names:CGI-95
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in membrane trafficking, specifically in membrane budding reactions By similarity.

Subunit structure

Binds STRN4 By similarity. Interacts with DNM1 and EPS15 By similarity. Interacts with nucleoside diphosphate kinase By similarity. Binds STRN and STRN3. Part of a ternary complex containing MOB4/PHOCN, STRN and/or STRN3 and PPA2. Interacts with CTTNBP2 By similarity. Interacts with CTTNBP2NL. Ref.12 Ref.13

Subcellular location

Cytoplasmperinuclear region. Membrane; Peripheral membrane protein. Golgi apparatusGolgi stack membrane; Peripheral membrane protein. Note: In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks. Ref.12

Post-translational modification

Phosphorylated on serine residues. Ref.12

Sequence similarities

Belongs to the MOB1/phocein family.

Sequence caution

The sequence AAP97221.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAB45697.1 differs from that shown. Reason: Frameshift at position 168.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCytoplasm
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 11251078. Source: UniProtKB

Golgi cisterna membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y3A3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y3A3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9Y3A3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     20-40: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225MOB-like protein phocein
PRO_0000193576

Sites

Metal binding921Zinc By similarity
Metal binding971Zinc By similarity
Metal binding1691Zinc By similarity
Metal binding1741Zinc By similarity

Natural variations

Alternative sequence1 – 3232Missing in isoform 2.
VSP_012303
Alternative sequence20 – 4021Missing in isoform 3.
VSP_041091

Experimental info

Sequence conflict901S → G in BAB15635. Ref.7
Sequence conflict1351L → P in CAB45697. Ref.6
Sequence conflict1721F → S in BAB15635. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: D0EF1219711458BA

FASTA22526,032
        10         20         30         40         50         60 
MVMAEGTAVL RRNRPGTKAQ DFYNWPDESF DEMDSTLAVQ QYIQQNIRAD CSNIDKILEP 

        70         80         90        100        110        120 
PEGQDEGVWK YEHLRQFCLE LNGLAVKLQS ECHPDTCTQM TATEQWIFLC AAHKTPKECP 

       130        140        150        160        170        180 
AIDYTRHTLD GAACLLNSNK YFPSRVSIKE SSVAKLGSVC RRIYRIFSHA YFHHRQIFDE 

       190        200        210        220 
YENETFLCHR FTKFVMKYNL MSKDNLIVPI LEEEVQNSVS GESEA 

« Hide

Isoform 2 [UniParc].

Checksum: 37E70D59A5437A7A
Show »

FASTA19322,318
Isoform 3 [UniParc].

Checksum: 52D1517EA742A30C
Show »

FASTA20423,513

References

« Hide 'large scale' references
[1]"Human MOB3."
Kagaya S., Todokoro K., Kotani S.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning of a novel human cDNA homology to murine B6D2F1 clone 2C4D mRNA."
Hu P.R., Yu L., Zhao Y., Yue P., Li M.Z., Zhao S.Y.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Characterization of the human Mob-1 like proteins."
Florindo C.S., Tavares A.A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Adrenal gland.
[6]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[7]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain and Trachea.
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[9]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Urinary bladder.
[12]"A mammalian homolog of yeast MOB1 is both a member and a putative substrate of striatin family-protein phosphatase 2A complexes."
Moreno C.S., Lane W.S., Pallas D.C.
J. Biol. Chem. 276:24253-24260(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 57-70 AND 166-175, INTERACTION WITH STRN; STRN3 AND THE PPA2 COMPLEX, PHOSPHORYLATION, SUBCELLULAR LOCATION.
[13]"A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein."
Goudreault M., D'Ambrosio L.M., Kean M.J., Mullin M.J., Larsen B.G., Sanchez A., Chaudhry S., Chen G.I., Sicheri F., Nesvizhskii A.I., Aebersold R., Raught B., Gingras A.C.
Mol. Cell. Proteomics 8:157-171(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CTTNBP2NL.
[14]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015441 mRNA. Translation: BAB19057.1.
AF093825 mRNA. Translation: AAP97221.1. Different initiation.
AJ580638 mRNA. Translation: CAE45270.1.
AF151853 mRNA. Translation: AAD34090.1.
AF250319 mRNA. Translation: AAG44567.1.
AL080070 mRNA. Translation: CAB45697.1. Frameshift.
AK027043 mRNA. Translation: BAB15635.1.
AK292938 mRNA. Translation: BAF85627.1.
AK297514 mRNA. Translation: BAG59922.1.
CR457371 mRNA. Translation: CAG33652.1.
AC020550 Genomic DNA. Translation: AAX93147.1.
CH471063 Genomic DNA. Translation: EAW70164.1.
CH471063 Genomic DNA. Translation: EAW70167.1.
BC005237 mRNA. Translation: AAH05237.1.
PIRT12466.
RefSeqNP_001094289.1. NM_001100819.2.
NP_001191023.1. NM_001204094.1.
NP_056202.2. NM_015387.4.
NP_955776.1. NM_199482.3.
UniGeneHs.633165.

3D structure databases

ProteinModelPortalQ9Y3A3.
SMRQ9Y3A3. Positions 61-196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117369. 63 interactions.
IntActQ9Y3A3. 62 interactions.
MINTMINT-1380326.
STRING9606.ENSP00000315702.

PTM databases

PhosphoSiteQ9Y3A3.

Polymorphism databases

DMDM56749365.

2D gel databases

OGPQ9Y3A3.

Proteomic databases

PaxDbQ9Y3A3.
PRIDEQ9Y3A3.

Protocols and materials databases

DNASU25843.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000233892; ENSP00000233892; ENSG00000115540. [Q9Y3A3-2]
ENST00000323303; ENSP00000315702; ENSG00000115540. [Q9Y3A3-1]
ENST00000409360; ENSP00000387289; ENSG00000115540. [Q9Y3A3-2]
ENST00000448447; ENSP00000405354; ENSG00000115540. [Q9Y3A3-3]
GeneID25843.
KEGGhsa:25843.
UCSCuc002uum.4. human. [Q9Y3A3-1]
uc010fsn.3. human. [Q9Y3A3-3]
uc021vum.1. human. [Q9Y3A3-2]

Organism-specific databases

CTD25843.
GeneCardsGC02P198383.
HGNCHGNC:17261. MOB4.
HPACAB034082.
HPA044125.
MIM609361. gene.
neXtProtNX_Q9Y3A3.
PharmGKBPA162396053.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG297181.
HOVERGENHBG052490.
InParanoidQ9Y3A3.
OMADEPFEEM.
OrthoDBEOG7VDXQ5.
PhylomeDBQ9Y3A3.
TreeFamTF314078.

Gene expression databases

ArrayExpressQ9Y3A3.
BgeeQ9Y3A3.
CleanExHS_MOBKL3.
GenevestigatorQ9Y3A3.

Family and domain databases

Gene3D1.20.140.30. 1 hit.
InterProIPR005301. Mob1_phocein.
[Graphical view]
PANTHERPTHR22599. PTHR22599. 1 hit.
PfamPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMSSF101152. SSF101152. 1 hit.
ProtoNetSearch...

Other

GeneWikiMOBKL3.
GenomeRNAi25843.
NextBio47167.
PROQ9Y3A3.
SOURCESearch...

Entry information

Entry namePHOCN_HUMAN
AccessionPrimary (citable) accession number: Q9Y3A3
Secondary accession number(s): B4DML0 expand/collapse secondary AC list , Q53SE0, Q7Z4Y6, Q9H2P3, Q9H5J1, Q9Y4T8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM