Reviewed,
UniProtKB/Swiss-Prot Q9Y385 (UB2J1_HUMAN)
Last modified
November 4, 2008.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ubiquitin-conjugating enzyme E2 J1 EC=6.3.2.19 Alternative name(s): Non-canonical ubiquitin-conjugating enzyme 1 Short name=NCUBE1 Yeast ubiquitin-conjugating enzyme UBC6 homolog E HSUBC6e | ||||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 318 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum. |
| Catalytic activity | ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type IV membrane protein. |
| Sequence similarities | Belongs to the ubiquitin-conjugating enzyme family. |
| Sequence caution | The sequence AAF36125.1 differs from that shown. Reason: Frameshift at position 119. |
Ontologies
Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Cellular component | Endoplasmic reticulum Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Transmembrane |
| Molecular function | Ligase |
| PTM | Phosphoprotein |
Gene Ontology (GO) | |
| Biological process | ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 318 | 318 | Ubiquitin-conjugating enzyme E2 J1 | PRO_0000082594 | |||||
Regions | |||||||||
| Topological domain | 1 – 282 | 282 | Cytoplasmic Potential | ||||||
| Transmembrane | 283 – 303 | 21 | Anchor for type IV membrane protein Potential | ||||||
| Topological domain | 304 – 318 | 15 | Lumenal Potential | ||||||
Sites | |||||||||
| Active site | 91 | 1 | Glycyl thioester intermediate | ||||||
Amino acid modifications | |||||||||
| Modified residue | 266 | 1 | Phosphoserine | ||||||
| Modified residue | 268 | 1 | Phosphoserine | ||||||
Natural variations | |||||||||
| Natural variant | 55 | 1 | G → V: dbSNP rs8099. | VAR_019689 | |||||
| Natural variant | 229 | 1 | L → V: dbSNP rs10502. | VAR_019690 | |||||
Experimental info | |||||||||
| Mutagenesis | 91 | 1 | C → S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER | ||||||
| Sequence conflict | 32 – 33 | 2 | QP → HA in AAD34071. Ref.3 | ||||||
| Sequence conflict | 276 | 1 | Q → H in AAF21505. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of a family of non-canonical ubiquitin-conjugating enzymes structurally related to yeast UBC6." Lester D.H., Farquharson C., Russell G.C., Houston B. Biochem. Biophys. Res. Commun. 269:474-480(2000) [PubMed: 10708578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "A role for mammalian Ubc6 homologues in ER-associated protein degradation." Lenk U., Yu H., Walter J., Gelman M.S., Hartmann E., Kopito R.R., Sommer T. J. Cell Sci. 115:3007-3014(2002) [PubMed: 12082160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-55, FUNCTION, MUTAGENESIS OF CYS-91, SUBCELLULAR LOCATION. |
| [3] | "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. Chen Z.Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-229. Tissue: Umbilical cord blood. |
| [5] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-229. Tissue: Muscle. |
| [7] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266 AND SER-268, MASS SPECTROMETRY. Tissue: Epithelium. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AJ245898 mRNA. Translation: CAB83212.1. U93243 mRNA. Translation: AAF21505.1. AF151039 mRNA. Translation: AAF36125.1. Frameshift. AF151834 mRNA. Translation: AAD34071.1. Different initiation. AF161502 mRNA. Translation: AAF29117.1. AL138717, AL139804 Genomic DNA. Translation: CAH70228.1. AL139804, AL138717 Genomic DNA. Translation: CAI19635.1. BC013973 mRNA. Translation: AAH13973.1. | |
| RefSeq | NP_057105.2. |
| UniGene | Hs.163776 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1QCQ based on UniProtKB P15731. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q9Y385. |
2-D gel databases | |
| OGP | Q9Y385. |
Proteomic databases | |
| PeptideAtlas | Q9Y385. |
Genome annotation databases | |
| Ensembl | ENSG00000198833. Homo sapiens. [Contig view] |
| GeneID | 51465. |
| KEGG | hsa:51465. |
Organism-specific databases | |
| H-InvDB | HIX0023025. |
| HGNC | HGNC:17598. UBE2J1. |
| HPA | HPA003509. |
| PharmGKB | PA134906541. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOVERGEN | Q9Y385. |
Gene expression databases | |
| ArrayExpress | Q9Y385. |
| CleanEx | HS_UBE2J1. |
| GermOnline | ENSG00000198833. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view] |
| Gene3D | G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit. |
| PANTHER | PTHR11621. UBQ-conjugat_E2. 1 hit. |
| Pfam | PF00179. UQ_con. 1 hit. [Graphical view] |
| ProDom | PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00212. UBCc. 1 hit. [Graphical view] |
| PROSITE | PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view] |
| BLOCKS | Search... |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 55093. |
Entry information
| Entry name | UB2J1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9Y385 Secondary accession number(s): Q5W0N4 Q9UF10 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

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