Reviewed, UniProtKB/Swiss-Prot Q9Y385 (UB2J1_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 J1
    EC=6.3.2.19
Alternative name(s):
    Non-canonical ubiquitin-conjugating enzyme 1
      Short name=NCUBE1
    Yeast ubiquitin-conjugating enzyme UBC6 homolog E
    HSUBC6e

Gene names

Name:	UBE2J1
Synonyms:	NCUBE1
ORF Names:	CGI-76, HSPC153, HSPC205

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type IV membrane protein.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAF36125.1 differs from that shown. Reason: Frameshift at position 119.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ubiquitin-conjugating enzyme E2 J1		PRO_0000082594
Regions
	Topological domain	1 – 282	282	Cytoplasmic Potential
	Transmembrane	283 – 303	21	Anchor for type IV membrane protein Potential
	Topological domain	304 – 318	15	Lumenal Potential
Sites
	Active site	91	1	Glycyl thioester intermediate
Amino acid modifications
	Modified residue	266	1	Phosphoserine
	Modified residue	268	1	Phosphoserine
Natural variations
	Natural variant	55	1	G → V: dbSNP rs8099.		VAR_019689
	Natural variant	229	1	L → V: dbSNP rs10502.		VAR_019690
Experimental info
	Mutagenesis	91	1	C → S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER
	Sequence conflict	32 – 33	2	QP → HA in AAD34071. Ref.3
	Sequence conflict	276	1	Q → H in AAF21505. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Y385-1 [UniParc]. Last modified September 13, 2004. Version 2. Checksum: CFF5FE00C2BBD39E	FASTA	318	35,199
	10 20 30 40 50 60 METRYNLKSP AVKRLMKEAA ELKDPTDHYH AQPLEDNLFE WHFTVRGPPD SDFDGGVYHG 70 80 90 100 110 120 RIVLPPEYPM KPPSIILLTA NGRFEVGKKI CLSISGHHPE TWQPSWSIRT ALLAIIGFMP 130 140 150 160 170 180 TKGEGAIGSL DYTPEERRAL AKKSQDFCCE GCGSAMKDVL LPLKSGSDSS QADQEAKELA 190 200 210 220 230 240 RQISFKAEVN SSGKTISESD LNHSFSLTDL QDDIPTTFQG ATASTSYGLQ NSSAASFHQP 250 260 270 280 290 300 TQPVAKNTSM SPRQRRAQQQ SQRRLSTSPD VIQGHQPRDN HTDHGGSAVL IVILTLALAA 310 LIFRRIYLAN EYIFDFEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a family of non-canonical ubiquitin-conjugating enzymes structurally related to yeast UBC6." Lester D.H., Farquharson C., Russell G.C., Houston B. Biochem. Biophys. Res. Commun. 269:474-480(2000) [PubMed: 10708578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A role for mammalian Ubc6 homologues in ER-associated protein degradation." Lenk U., Yu H., Walter J., Gelman M.S., Hartmann E., Kopito R.R., Sommer T. J. Cell Sci. 115:3007-3014(2002) [PubMed: 12082160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-55, FUNCTION, MUTAGENESIS OF CYS-91, SUBCELLULAR LOCATION.
[3]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-229. Tissue: Umbilical cord blood.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-229. Tissue: Muscle.
[7]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266 AND SER-268, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ245898 mRNA. Translation: CAB83212.1. U93243 mRNA. Translation: AAF21505.1. AF151039 mRNA. Translation: AAF36125.1. Frameshift. AF151834 mRNA. Translation: AAD34071.1. Different initiation. AF161502 mRNA. Translation: AAF29117.1. AL138717, AL139804 Genomic DNA. Translation: CAH70228.1. AL139804, AL138717 Genomic DNA. Translation: CAI19635.1. BC013973 mRNA. Translation: AAH13973.1.
RefSeq	NP_057105.2.
UniGene	Hs.163776
3D structure databases
HSSP	HSSP built from PDB template 1QCQ based on UniProtKB P15731.
ModBase	Search...
PTM databases
PhosphoSite	Q9Y385.
2-D gel databases
OGP	Q9Y385.
Proteomic databases
PeptideAtlas	Q9Y385.
Genome annotation databases
Ensembl	ENSG00000198833. Homo sapiens. [Contig view]
GeneID	51465.
KEGG	hsa:51465.
Organism-specific databases
H-InvDB	HIX0023025.
HGNC	HGNC:17598. UBE2J1.
HPA	HPA003509.
PharmGKB	PA134906541.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q9Y385.
Gene expression databases
ArrayExpress	Q9Y385.
CleanEx	HS_UBE2J1.
GermOnline	ENSG00000198833. Homo sapiens.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
NextBio	55093.

Entry information

Entry name

UB2J1_HUMAN

Accession

Primary (citable) accession number: Q9Y385
Secondary accession number(s): Q5W0N4 , Q9BZ32, Q9NQL3, Q9NY66, Q9P011, Q9P0S0, Q9UF10

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	September 13, 2004
Last modified:	November 4, 2008
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)