Reviewed, UniProtKB/Swiss-Prot Q9Y383 (LC7L2_HUMAN)

Names and origin

Protein names

Recommended name:
    Putative RNA-binding protein Luc7-like 2

Gene names

Name:	LUC7L2
ORF Names:	CGI-59, CGI-74

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May bind to RNA via its Arg/Ser-rich domain.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4 Ref.5 Ref.6 Ref.7
Sequence similarities	Belongs to the Luc7 family. Contains 1 C2H2-type zinc finger.
Sequence caution	The sequence AAD34069.1 differs from that shown. Reason: Frameshift at position 388.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RNPS1	Q15287	1	EBI-352851,EBI-395959
YWHAG	P61981	1	EBI-352851,EBI-359832
YWHAQ	P27348	1	EBI-352851,EBI-359854

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y383-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y383-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MSAQAQMRAMLDQLMGTSRDG → MPAYLNLQGSVRKAPHSPSR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Putative RNA-binding protein Luc7-like 2		PRO_0000187282
Regions
	Zinc finger	188 – 215	28	C2H2-type; atypical
	Coiled coil	102 – 177	76	Potential
	Compositional bias	239 – 388	150	Arg/Ser-rich
Amino acid modifications
	Modified residue	17	1	Phosphothreonine Ref.5
	Modified residue	18	1	Phosphoserine Ref.5 Ref.7
	Modified residue	111	1	Phosphothreonine Ref.6
	Modified residue	354	1	Phosphoserine Ref.5
	Modified residue	358	1	Phosphoserine Ref.4 Ref.5
	Modified residue	383	1	Phosphoserine Ref.7
	Modified residue	384	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 21	21	MSAQA…TSRDG → MPAYLNLQGSVRKAPHSPSR in isoform 2.		VSP_010217
	Natural variant	361	1	D → E: dbSNP rs3757435.		VAR_034067
Experimental info
	Sequence conflict	27	1	R → Q in BAA91737. Ref.2
	Sequence conflict	389 – 390	2	AG → QR in AAD34054. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 1C559CCE0F23F693	FASTA	392	46,514
	10 20 30 40 50 60 MSAQAQMRAM LDQLMGTSRD GDTTRQRIKF SDDRVCKSHL LNCCPHDVLS GTRMDLGECL 70 80 90 100 110 120 KVHDLALRAD YEIASKEQDF FFELDAMDHL QSFIADCDRR TEVAKKRLAE TQEEISAEVA 130 140 150 160 170 180 AKAERVHELN EEIGKLLAKV EQLGAEGNVE ESQKVMDEVE KARAKKREAE EVYRNSMPAS 190 200 210 220 230 240 SFQQQKLRVC EVCSAYLGLH DNDRRLADHF GGKLHLGFIE IREKLEELKR VVAEKQEKRN 250 260 270 280 290 300 QERLKRREER EREEREKLRR SRSHSKNPKR SRSREHRRHR SRSMSRERKR RTRSKSREKR 310 320 330 340 350 360 HRHRSRSSSR SRSRSHQRSR HSSRDRSRER SKRRSSKERF RDQDLASCDR DRSSRDRSPR 370 380 390 DRDRKDKKRS YESANGRSED RRSSEEREAG EI
	Isoform 2. Checksum: 68FBCFE447A3E0E5	FASTA	391	46,425
	10 20 30 40 50 60 MPAYLNLQGS VRKAPHSPSR DTTRQRIKFS DDRVCKSHLL NCCPHDVLSG TRMDLGECLK 70 80 90 100 110 120 VHDLALRADY EIASKEQDFF FELDAMDHLQ SFIADCDRRT EVAKKRLAET QEEISAEVAA 130 140 150 160 170 180 KAERVHELNE EIGKLLAKVE QLGAEGNVEE SQKVMDEVEK ARAKKREAEE VYRNSMPASS 190 200 210 220 230 240 FQQQKLRVCE VCSAYLGLHD NDRRLADHFG GKLHLGFIEI REKLEELKRV VAEKQEKRNQ 250 260 270 280 290 300 ERLKRREERE REEREKLRRS RSHSKNPKRS RSREHRRHRS RSMSRERKRR TRSKSREKRH 310 320 330 340 350 360 RHRSRSSSRS RSRSHQRSRH SSRDRSRERS KRRSSKERFR DQDLASCDRD RSSRDRSPRD 370 380 390 RDRKDKKRSY ESANGRSEDR RSSEEREAGE I

References

[1]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Skin and Uterus.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17; SER-18; SER-354 AND SER-358, MASS SPECTROMETRY.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-111, MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-383 AND SER-384, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF151817 mRNA. Translation: AAD34054.1. AF151832 mRNA. Translation: AAD34069.1. Frameshift. AK001476 mRNA. Translation: BAA91713.1. AK001519 mRNA. Translation: BAA91737.1. AK022895 mRNA. Translation: BAB14297.1. BC017163 mRNA. Translation: AAH17163.1. BC042625 mRNA. Translation: AAH42625.1. BC050708 mRNA. Translation: AAH50708.1. BC056886 mRNA. Translation: AAH56886.1.
IPI	IPI00006932. IPI00216804.
RefSeq	NP_057103.2.
UniGene	Hs.370475
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y383. 38 interactions.
PTM databases
PhosphoSite	Q9Y383.
Proteomic databases
PRIDE	Q9Y383.
Genome annotation databases
Ensembl	ENSG00000146963. Homo sapiens. [Contig view]
GeneID	51631.
KEGG	hsa:51631.
UCSC	uc003vux.1. human. uc003vuy.1. human.
Organism-specific databases
GeneCards	GC07P138695.
HGNC	HGNC:21608. LUC7L2.
PharmGKB	PA134873425.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9Y383.
HOVERGEN	Q9Y383.
OMA	Q9Y383. NPKRSRS.
Gene expression databases
ArrayExpress	Q9Y383.
Bgee	Q9Y383.
CleanEx	HS_LUC7L2.
GermOnline	ENSG00000146963. Homo sapiens.
Family and domain databases
InterPro	IPR004882. LUC7_rel. [Graphical view]
PANTHER	PTHR12375. LUC7_rel. 1 hit.
Pfam	PF03194. LUC7. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	55557.
PMAP-CutDB	Q9Y383.

Entry information

Entry name

LC7L2_HUMAN

Accession

Primary (citable) accession number: Q9Y383
Secondary accession number(s): Q8IUP9 , Q9NVL3, Q9NVN7, Q9UQN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	July 7, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)