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Protein

Calcium-binding protein 39

Gene

CAB39

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1.1 Publication

GO - Molecular functioni

  1. kinase binding Source: BHF-UCL
  2. protein kinase activator activity Source: UniProtKB
  3. protein serine/threonine kinase activator activity Source: ParkinsonsUK-UCL
  4. protein serine/threonine kinase activity Source: Ensembl

GO - Biological processi

  1. activation of protein kinase activity Source: Ensembl
  2. cell cycle arrest Source: Reactome
  3. cellular hypotonic response Source: ParkinsonsUK-UCL
  4. insulin receptor signaling pathway Source: Reactome
  5. intracellular signal transduction Source: ParkinsonsUK-UCL
  6. negative regulation of potassium ion transmembrane transport Source: ParkinsonsUK-UCL
  7. negative regulation of potassium ion transmembrane transporter activity Source: ParkinsonsUK-UCL
  8. negative regulation of rubidium ion transmembrane transporter activity Source: ParkinsonsUK-UCL
  9. negative regulation of rubidium ion transport Source: ParkinsonsUK-UCL
  10. positive regulation of peptidyl-serine phosphorylation Source: ParkinsonsUK-UCL
  11. positive regulation of peptidyl-threonine phosphorylation Source: ParkinsonsUK-UCL
  12. positive regulation of protein serine/threonine kinase activity Source: ParkinsonsUK-UCL
  13. protein heterooligomerization Source: Ensembl
  14. signal transduction by phosphorylation Source: ParkinsonsUK-UCL
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_21285. Regulation of AMPK activity via LKB1.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein 39
Alternative name(s):
MO25alpha
Protein Mo25
Gene namesi
Name:CAB39
Synonyms:MO25
ORF Names:CGI-66
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:20292. CAB39.

Subcellular locationi

  1. Cytoplasm Curated

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. extracellular vesicular exosome Source: UniProtKB
  3. serine/threonine protein kinase complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi240 – 2401R → A: Abolishes activation of STK11/LKB1; when associated with A-243. 1 Publication
Mutagenesisi243 – 2431F → A: Abolishes activation of STK11/LKB1; when associated with A-240. 1 Publication

Organism-specific databases

PharmGKBiPA128394663.

Polymorphism and mutation databases

BioMutaiCAB39.
DMDMi15214082.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Calcium-binding protein 39PRO_0000209824Add
BLAST

Proteomic databases

MaxQBiQ9Y376.
PaxDbiQ9Y376.
PeptideAtlasiQ9Y376.
PRIDEiQ9Y376.

PTM databases

PhosphoSiteiQ9Y376.

Expressioni

Gene expression databases

BgeeiQ9Y376.
CleanExiHS_CAB39.
ExpressionAtlasiQ9Y376. baseline and differential.
GenevestigatoriQ9Y376.

Organism-specific databases

HPAiCAB037112.

Interactioni

Subunit structurei

Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
OXSR1O957473EBI-306905,EBI-620853
STK11Q158315EBI-306905,EBI-306838
STK25O005063EBI-306905,EBI-618295
STK26Q9P2896EBI-306905,EBI-618239
STK39Q9UEW84EBI-306905,EBI-2680974
STRADAQ7RTN63EBI-306905,EBI-1109114
STRADBQ9C0K75EBI-306905,EBI-306893

Protein-protein interaction databases

BioGridi119696. 21 interactions.
DIPiDIP-31316N.
IntActiQ9Y376. 11 interactions.
MINTiMINT-5000103.
STRINGi9606.ENSP00000258418.

Structurei

Secondary structure

1
341
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 2716Combined sources
Beta strandi29 – 313Combined sources
Helixi34 – 5421Combined sources
Beta strandi58 – 614Combined sources
Helixi64 – 7714Combined sources
Helixi79 – 857Combined sources
Helixi87 – 893Combined sources
Helixi92 – 10615Combined sources
Helixi115 – 1217Combined sources
Helixi125 – 1328Combined sources
Helixi133 – 1353Combined sources
Turni137 – 1393Combined sources
Helixi140 – 15112Combined sources
Helixi154 – 1629Combined sources
Helixi164 – 1674Combined sources
Helixi168 – 1725Combined sources
Helixi178 – 19316Combined sources
Helixi196 – 20510Combined sources
Helixi207 – 21711Combined sources
Helixi223 – 23816Combined sources
Helixi240 – 2423Combined sources
Helixi243 – 2497Combined sources
Helixi253 – 26210Combined sources
Helixi268 – 28316Combined sources
Helixi289 – 2979Combined sources
Helixi299 – 30810Combined sources
Turni309 – 3124Combined sources
Helixi314 – 3163Combined sources
Helixi318 – 33114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UPKX-ray1.85A1-341[»]
1UPLX-ray2.60A/B1-341[»]
2WTKX-ray2.65A/D1-341[»]
3GNIX-ray2.35A1-341[»]
4FZAX-ray3.15A11-334[»]
4FZDX-ray3.25A11-334[»]
4FZFX-ray3.64A11-334[»]
4NZWX-ray3.58A8-334[»]
4O27X-ray3.18A11-333[»]
ProteinModelPortaliQ9Y376.
SMRiQ9Y376. Positions 2-336.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y376.

Family & Domainsi

Sequence similaritiesi

Belongs to the Mo25 family.Curated

Phylogenomic databases

eggNOGiNOG307443.
GeneTreeiENSGT00390000004360.
HOGENOMiHOG000234711.
HOVERGENiHBG050757.
InParanoidiQ9Y376.
KOiK08272.
OMAiCAEFLEA.
OrthoDBiEOG7CK371.
PhylomeDBiQ9Y376.
TreeFamiTF314910.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013878. Mo25.
[Graphical view]
PANTHERiPTHR10182. PTHR10182. 1 hit.
PfamiPF08569. Mo25. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y376-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFPFGKSHK SPADIVKNLK ESMAVLEKQD ISDKKAEKAT EEVSKNLVAM
60 70 80 90 100
KEILYGTNEK EPQTEAVAQL AQELYNSGLL STLVADLQLI DFEGKKDVAQ
110 120 130 140 150
IFNNILRRQI GTRTPTVEYI CTQQNILFML LKGYESPEIA LNCGIMLREC
160 170 180 190 200
IRHEPLAKII LWSEQFYDFF RYVEMSTFDI ASDAFATFKD LLTRHKLLSA
210 220 230 240 250
EFLEQHYDRF FSEYEKLLHS ENYVTKRQSL KLLGELLLDR HNFTIMTKYI
260 270 280 290 300
SKPENLKLMM NLLRDKSRNI QFEAFHVFKV FVANPNKTQP ILDILLKNQA
310 320 330 340
KLIEFLSKFQ NDRTEDEQFN DEKTYLVKQI RDLKRPAQQE A
Length:341
Mass (Da):39,869
Last modified:November 1, 1999 - v1
Checksum:iEC710A528B6F9811
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151824 mRNA. Translation: AAD34061.1.
AF113536 mRNA. Translation: AAF14873.1.
AK292382 mRNA. Translation: BAF85071.1.
AK315692 mRNA. Translation: BAG38055.1.
CH471063 Genomic DNA. Translation: EAW70932.1.
BC020570 mRNA. Translation: AAH20570.1.
CCDSiCCDS2478.1.
RefSeqiNP_001124321.1. NM_001130849.1.
NP_001124322.1. NM_001130850.1.
NP_057373.1. NM_016289.3.
UniGeneiHs.603930.

Genome annotation databases

EnsembliENST00000258418; ENSP00000258418; ENSG00000135932.
ENST00000409788; ENSP00000386238; ENSG00000135932.
ENST00000410084; ENSP00000386642; ENSG00000135932.
GeneIDi51719.
KEGGihsa:51719.
UCSCiuc002vqx.3. human.

Polymorphism and mutation databases

BioMutaiCAB39.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151824 mRNA. Translation: AAD34061.1.
AF113536 mRNA. Translation: AAF14873.1.
AK292382 mRNA. Translation: BAF85071.1.
AK315692 mRNA. Translation: BAG38055.1.
CH471063 Genomic DNA. Translation: EAW70932.1.
BC020570 mRNA. Translation: AAH20570.1.
CCDSiCCDS2478.1.
RefSeqiNP_001124321.1. NM_001130849.1.
NP_001124322.1. NM_001130850.1.
NP_057373.1. NM_016289.3.
UniGeneiHs.603930.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UPKX-ray1.85A1-341[»]
1UPLX-ray2.60A/B1-341[»]
2WTKX-ray2.65A/D1-341[»]
3GNIX-ray2.35A1-341[»]
4FZAX-ray3.15A11-334[»]
4FZDX-ray3.25A11-334[»]
4FZFX-ray3.64A11-334[»]
4NZWX-ray3.58A8-334[»]
4O27X-ray3.18A11-333[»]
ProteinModelPortaliQ9Y376.
SMRiQ9Y376. Positions 2-336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119696. 21 interactions.
DIPiDIP-31316N.
IntActiQ9Y376. 11 interactions.
MINTiMINT-5000103.
STRINGi9606.ENSP00000258418.

PTM databases

PhosphoSiteiQ9Y376.

Polymorphism and mutation databases

BioMutaiCAB39.
DMDMi15214082.

Proteomic databases

MaxQBiQ9Y376.
PaxDbiQ9Y376.
PeptideAtlasiQ9Y376.
PRIDEiQ9Y376.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000258418; ENSP00000258418; ENSG00000135932.
ENST00000409788; ENSP00000386238; ENSG00000135932.
ENST00000410084; ENSP00000386642; ENSG00000135932.
GeneIDi51719.
KEGGihsa:51719.
UCSCiuc002vqx.3. human.

Organism-specific databases

CTDi51719.
GeneCardsiGC02P231577.
HGNCiHGNC:20292. CAB39.
HPAiCAB037112.
MIMi612174. gene.
neXtProtiNX_Q9Y376.
PharmGKBiPA128394663.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG307443.
GeneTreeiENSGT00390000004360.
HOGENOMiHOG000234711.
HOVERGENiHBG050757.
InParanoidiQ9Y376.
KOiK08272.
OMAiCAEFLEA.
OrthoDBiEOG7CK371.
PhylomeDBiQ9Y376.
TreeFamiTF314910.

Enzyme and pathway databases

ReactomeiREACT_21285. Regulation of AMPK activity via LKB1.

Miscellaneous databases

ChiTaRSiCAB39. human.
EvolutionaryTraceiQ9Y376.
GeneWikiiCAB39.
GenomeRNAii51719.
NextBioi55768.
PROiQ9Y376.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y376.
CleanExiHS_CAB39.
ExpressionAtlasiQ9Y376. baseline and differential.
GenevestigatoriQ9Y376.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013878. Mo25.
[Graphical view]
PANTHERiPTHR10182. PTHR10182. 1 hit.
PfamiPF08569. Mo25. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "A novel gene expressed in the human hypothalamus."
    Jin W., Shi J., Ren S., Gu J., Fu S., Huang Q., Dong H., Yu Y., Fu G., Wang Y., Chen Z., Han Z.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hypothalamus.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Duodenum.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "Crystal structure of MO25 alpha in complex with the C-terminus of the pseudo kinase STE20-related adaptor."
    Milburn C.C., Boudeau J., Deak M., Alessi D.R., van Aalten D.M.
    Nat. Struct. Mol. Biol. 11:193-200(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 10-339.
  9. "Structure of the LKB1-STRAD-MO25 complex reveals an allosteric mechanism of kinase activation."
    Zeqiraj E., Filippi B.M., Deak M., Alessi D.R., van Aalten D.M.
    Science 326:1707-1711(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 1-341 IN COMPLEX WITH STK11/LKB1 AND STRADA, IDENTIFICATION IN A COMPLEX WITH STK11/LKB1 AND STRADA, FUNCTION, MUTAGENESIS OF ARG-240 AND PHE-243.

Entry informationi

Entry nameiCAB39_HUMAN
AccessioniPrimary (citable) accession number: Q9Y376
Secondary accession number(s): A8K8L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1999
Last modified: April 29, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.