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Q9Y337 (KLK5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-5

EC=3.4.21.-
Alternative name(s):
Kallikrein-like protein 2
Short name=KLK-L2
Stratum corneum tryptic enzyme
Gene names
Name:KLK5
Synonyms:SCTE
ORF Names:UNQ570/PRO1132
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in desquamation.

Enzyme regulation

Inhibited by Zn2+. Ref.12

Subunit structure

Interacts with SPINK9. Ref.10

Subcellular location

Secreted.

Tissue specificity

Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. Ref.11

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 293271Kallikrein-5
PRO_0000027939

Regions

Domain67 – 290224Peptidase S1

Sites

Active site1081Charge relay system Ref.12
Active site1531Charge relay system Ref.12
Active site2451Charge relay system Ref.12
Site1501Major binding site for inhibitory zinc

Amino acid modifications

Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Ref.12
Glycosylation2521N-linked (GlcNAc...) Potential
Disulfide bond73 ↔ 206 Ref.12
Disulfide bond93 ↔ 109 Ref.12
Disulfide bond178 ↔ 279 Ref.12
Disulfide bond185 ↔ 251 Ref.12
Disulfide bond217 ↔ 231 Ref.12
Disulfide bond241 ↔ 266 Ref.12

Natural variations

Natural variant551G → R.
Corresponds to variant rs2232532 [ dbSNP | Ensembl ].
VAR_051853
Natural variant1531N → D. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Ref.9
Corresponds to variant rs183854 [ dbSNP | Ensembl ].
VAR_051854

Experimental info

Sequence conflict25 – 5632Missing in AAG33358. Ref.3

Secondary structure

.......................................... 293
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Y337 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: D92C9F98055131E4

FASTA29332,019
        10         20         30         40         50         60 
MATARPPWMW VLCALITALL LGVTEHVLAN NDVSCDHPSN TVPSGSNQDL GAGAGEDARS 

        70         80         90        100        110        120 
DDSSSRIING SDCDMHTQPW QAALLLRPNQ LYCGAVLVHP QWLLTAAHCR KKVFRVRLGH 

       130        140        150        160        170        180 
YSLSPVYESG QQMFQGVKSI PHPGYSHPGH SNNLMLIKLN RRIRPTKDVR PINVSSHCPS 

       190        200        210        220        230        240 
AGTKCLVSGW GTTKSPQVHF PKVLQCLNIS VLSQKRCEDA YPRQIDDTMF CAGDKAGRDS 

       250        260        270        280        290 
CQGDSGGPVV CNGSLQGLVS WGDYPCARPN RPGVYTNLCK FTKWIQETIQ ANS 

« Hide

References

« Hide 'large scale' references
[1]"Purification, molecular cloning, and expression of a human stratum corneum trypsin-like serine protease with possible function in desquamation."
Brattsand M., Egelrud T.
J. Biol. Chem. 274:30033-30040(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-153.
Tissue: Stratum corneum.
[2]"Identification of novel human kallikrein-like genes on chromosome 19q13.3-q13.4."
Yousef G.M., Luo L.-Y., Diamandis E.P.
Anticancer Res. 19:2843-2852(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASP-153.
[3]"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region."
Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K.
Gene 257:119-130(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASP-153.
[4]"Differential expression of a human kallikrein 5 (KLK5) splice variant in ovarian and prostate cancer."
Kurlender L., Yousef G.M., Memari N., Robb J.D., Michael I.P., Borgono C., Katsaros D., Stephan C., Jung K., Diamandis E.P.
Tumor Biol. 25:149-156(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-153.
[5]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-153.
[6]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-153.
[7]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASP-153.
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-153.
Tissue: Ovary.
[10]"SPINK9: a selective, skin-specific Kazal-type serine protease inhibitor."
Brattsand M., Stefansson K., Hubiche T., Nilsson S.K., Egelrud T.
J. Invest. Dermatol. 129:1656-1665(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPINK9.
[11]"Identification of lympho-epithelial Kazal-type inhibitor 2 in human skin as a kallikrein-related peptidase 5-specific protease inhibitor."
Meyer-Hoffert U., Wu Z., Schroeder J.M.
PLoS ONE 4:E4372-E4372(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[12]"Structural basis of the zinc inhibition of human tissue kallikrein 5."
Debela M., Goettig P., Magdolen V., Huber R., Schechter N.M., Bode W.
J. Mol. Biol. 373:1017-1031(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 67-293 ALONE AND IN COMPLEX WITH ZINC, ENZYME REGULATION, ACTIVE SITE, GLYCOSYLATION AT ASN-208, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF168768 mRNA. Translation: AAF03101.1.
AF135028 Genomic DNA. Translation: AAD26429.1.
AF243527 Genomic DNA. Translation: AAG33358.1.
AY279380 mRNA. Translation: AAP42275.1.
AY279381 mRNA. Translation: AAP42276.1.
AY359010 mRNA. Translation: AAQ89369.1.
BT006867 mRNA. Translation: AAP35513.1.
AC011483 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71946.1.
BC008036 mRNA. Translation: AAH08036.1.
CCDSCCDS12810.1.
RefSeqNP_001070959.1. NM_001077491.1.
NP_001070960.1. NM_001077492.1.
NP_036559.1. NM_012427.4.
UniGeneHs.50915.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2PSXX-ray2.30A67-293[»]
2PSYX-ray2.30A67-293[»]
ProteinModelPortalQ9Y337.
SMRQ9Y337. Positions 35-293.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117346. 1 interaction.
STRING9606.ENSP00000337733.

Chemistry

ChEMBLCHEMBL4447.

Protein family/group databases

MEROPSS01.017.

PTM databases

PhosphoSiteQ9Y337.

Polymorphism databases

DMDM296434569.

Proteomic databases

PaxDbQ9Y337.
PRIDEQ9Y337.

Protocols and materials databases

DNASU25818.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336334; ENSP00000337733; ENSG00000167754.
ENST00000391809; ENSP00000375685; ENSG00000167754.
ENST00000593428; ENSP00000471966; ENSG00000167754.
GeneID25818.
KEGGhsa:25818.
UCSCuc002pue.3. human.

Organism-specific databases

CTD25818.
GeneCardsGC19M051446.
HGNCHGNC:6366. KLK5.
HPACAB025503.
CAB026341.
HPA014343.
MIM605643. gene.
neXtProtNX_Q9Y337.
PharmGKBPA30155.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ9Y337.
KOK09617.
OMAAAHCRKK.
OrthoDBEOG75B84T.
PhylomeDBQ9Y337.
TreeFamTF331065.

Gene expression databases

ArrayExpressQ9Y337.
BgeeQ9Y337.
CleanExHS_KLK5.
GenevestigatorQ9Y337.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9Y337.
GeneWikiKLK5.
GenomeRNAi25818.
NextBio47059.
PMAP-CutDBQ9Y337.
PROQ9Y337.
SOURCESearch...

Entry information

Entry nameKLK5_HUMAN
AccessionPrimary (citable) accession number: Q9Y337
Secondary accession number(s): Q53ZR3, Q9HBG8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM