Q9Y337 (KLK5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein-5
EC=3.4.21.-

Alternative name(s):
Kallikrein-like protein 2
Short name=KLK-L2
Stratum corneum tryptic enzyme

Gene names

Name:	KLK5
Synonyms:	SCTE
ORF Names:	UNQ570/PRO1132

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in desquamation.
Enzyme regulation	Inhibited by Zn2+. Ref.12
Subunit structure	Interacts with SPINK9. Ref.10
Subcellular location	Secreted.
Tissue specificity	Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. Ref.11
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	epidermis development Traceable author statement Ref.1. Source: ProtInc positive regulation of G-protein coupled receptor protein signaling pathway Inferred from direct assay PubMed 16885167. Source: UniProtKB proteolysis Non-traceable author statement. Source: UniProtKB
Cellular_component	epidermal lamellar body Inferred from direct assay PubMed 15675955. Source: UniProtKB extracellular space Traceable author statement PubMed 10608802. Source: ProtInc
Molecular_function	serine-type endopeptidase activity Inferred from direct assay PubMed 16885167. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Potential

Chain

23 – 293

271

Kallikrein-5

PRO_0000027939

Regions

Domain

67 – 290

224

Peptidase S1

Sites

Active site

108

1

Charge relay system Ref.12

Active site

153

1

Charge relay system Ref.12

Active site

245

1

Charge relay system Ref.12

Site

150

1

Major binding site for inhibitory zinc

Amino acid modifications

Glycosylation

69

1

N-linked (GlcNAc...) Potential

Glycosylation

173

1

N-linked (GlcNAc...) Potential

Glycosylation

208

1

N-linked (GlcNAc...) Ref.12

Glycosylation

252

1

N-linked (GlcNAc...) Potential

Disulfide bond

73 ↔ 206

Ref.12

Disulfide bond

93 ↔ 109

Ref.12

Disulfide bond

178 ↔ 279

Ref.12

Disulfide bond

185 ↔ 251

Ref.12

Disulfide bond

217 ↔ 231

Ref.12

Disulfide bond

241 ↔ 266

Ref.12

Natural variations

Natural variant

55

1

G → R.
Corresponds to variant rs2232532 [ dbSNP | Ensembl ].

VAR_051853

Natural variant

153

1

N → D. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Ref.9
Corresponds to variant rs183854 [ dbSNP | Ensembl ].

VAR_051854

Experimental info

Sequence conflict

25 – 56

32

Missing in AAG33358. Ref.3

Secondary structure

1

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293

Helix Strand Turn

Details...

Beta strand

81 – 86

6

Turn

87 – 89

3

Beta strand

90 – 99

10

Beta strand

102 – 105

4

Helix

107 – 109

3

Beta strand

115 – 119

5

Beta strand

122 – 124

3

Beta strand

132 – 141

10

Beta strand

147 – 149

3

Beta strand

155 – 161

7

Beta strand

167 – 169

3

Beta strand

184 – 191

8

Beta strand

193 – 197

5

Beta strand

205 – 211

7

Helix

214 – 220

7

Turn

222 – 224

3

Beta strand

229 – 232

4

Beta strand

248 – 251

4

Beta strand

254 – 261

8

Beta strand

273 – 277

5

Helix

278 – 280

3

Helix

282 – 291

10

Sequences

	Sequence		Length	Mass (Da)
	Q9Y337 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: D92C9F98055131E4	FASTA	293	32,019
	10 20 30 40 50 60 MATARPPWMW VLCALITALL LGVTEHVLAN NDVSCDHPSN TVPSGSNQDL GAGAGEDARS 70 80 90 100 110 120 DDSSSRIING SDCDMHTQPW QAALLLRPNQ LYCGAVLVHP QWLLTAAHCR KKVFRVRLGH 130 140 150 160 170 180 YSLSPVYESG QQMFQGVKSI PHPGYSHPGH SNNLMLIKLN RRIRPTKDVR PINVSSHCPS 190 200 210 220 230 240 AGTKCLVSGW GTTKSPQVHF PKVLQCLNIS VLSQKRCEDA YPRQIDDTMF CAGDKAGRDS 250 260 270 280 290 CQGDSGGPVV CNGSLQGLVS WGDYPCARPN RPGVYTNLCK FTKWIQETIQ ANS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, molecular cloning, and expression of a human stratum corneum trypsin-like serine protease with possible function in desquamation." Brattsand M., Egelrud T. J. Biol. Chem. 274:30033-30040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-153. Tissue: Stratum corneum.
[2]	"Identification of novel human kallikrein-like genes on chromosome 19q13.3-q13.4." Yousef G.M., Luo L.-Y., Diamandis E.P. Anticancer Res. 19:2843-2852(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASP-153.
[3]	"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region." Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K. Gene 257:119-130(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASP-153.
[4]	"Differential expression of a human kallikrein 5 (KLK5) splice variant in ovarian and prostate cancer." Kurlender L., Yousef G.M., Memari N., Robb J.D., Michael I.P., Borgono C., Katsaros D., Stephan C., Jung K., Diamandis E.P. Tumor Biol. 25:149-156(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-153.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-153.
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-153.
[7]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASP-153.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-153. Tissue: Ovary.
[10]	"SPINK9: a selective, skin-specific Kazal-type serine protease inhibitor." Brattsand M., Stefansson K., Hubiche T., Nilsson S.K., Egelrud T. J. Invest. Dermatol. 129:1656-1665(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SPINK9.
[11]	"Identification of lympho-epithelial Kazal-type inhibitor 2 in human skin as a kallikrein-related peptidase 5-specific protease inhibitor." Meyer-Hoffert U., Wu Z., Schroeder J.M. PLoS ONE 4:E4372-E4372(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[12]	"Structural basis of the zinc inhibition of human tissue kallikrein 5." Debela M., Goettig P., Magdolen V., Huber R., Schechter N.M., Bode W. J. Mol. Biol. 373:1017-1031(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 67-293 ALONE AND IN COMPLEX WITH ZINC, ENZYME REGULATION, ACTIVE SITE, GLYCOSYLATION AT ASN-208, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF168768 mRNA. Translation: AAF03101.1.
AF135028 Genomic DNA. Translation: AAD26429.1.
AF243527 Genomic DNA. Translation: AAG33358.1.
AY279380 mRNA. Translation: AAP42275.1.
AY279381 mRNA. Translation: AAP42276.1.
AY359010 mRNA. Translation: AAQ89369.1.
BT006867 mRNA. Translation: AAP35513.1.
AC011483 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71946.1.
BC008036 mRNA. Translation: AAH08036.1.

IPI

IPI00032464.

RefSeq

NP_001070959.1. NM_001077491.1.
NP_001070960.1. NM_001077492.1.
NP_036559.1. NM_012427.4.

UniGene

Hs.50915.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PSX	X-ray	2.30	A	67-293	[»]
2PSY	X-ray	2.30	A	67-293	[»]

ProteinModelPortal

Q9Y337.

SMR

Q9Y337. Positions 35-293.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000337733.

Protein family/group databases

MEROPS

S01.017.

PTM databases

PhosphoSite

Q9Y337.

Polymorphism databases

DMDM

296434569.

Proteomic databases

PaxDb

Q9Y337.

PRIDE

Q9Y337.

Protocols and materials databases

DNASU

25818.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000336334; ENSP00000337733; ENSG00000167754.
ENST00000391809; ENSP00000375685; ENSG00000167754.
ENST00000593428; ENSP00000471966; ENSG00000167754.

GeneID

25818.

KEGG

hsa:25818.

Organism-specific databases

CTD

25818.

GeneCards

GC19M051446.

HGNC

HGNC:6366. KLK5.

HPA

CAB025503.
CAB026341.
HPA014343.

MIM

605643. gene.

neXtProt

NX_Q9Y337.

PharmGKB

PA30155.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5640.

HOGENOM

HOG000251820.

HOVERGEN

HBG013304.

InParanoid

Q9Y337.

KO

K09617.

OMA

YESGQQM.

OrthoDB

EOG40K80B.

PhylomeDB

Q9Y337.

Gene expression databases

Bgee

Q9Y337.

CleanEx

HS_KLK5.

Genevestigator

Q9Y337.

GermOnline

ENSG00000167754. Homo sapiens.

Family and domain databases

InterPro

IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL4447.

EvolutionaryTrace

Q9Y337.

GenomeRNAi

25818.

NextBio

47059.

PMAP-CutDB

Q9Y337.

SOURCE

Search...

Entry information

Entry name

KLK5_HUMAN

Accession

Primary (citable) accession number: Q9Y337
Secondary accession number(s): Q53ZR3, Q9HBG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.