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Q9Y336 (SIGL9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialic acid-binding Ig-like lectin 9

Short name=Siglec-9
Alternative name(s):
CDw329
Protein FOAP-9
CD_antigen=CD329
Gene names
Name:SIGLEC9
ORF Names:UNQ668/PRO1302
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon.

Domain

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Sequence similarities

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   LigandLectin
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface receptor signaling pathway

Non-traceable author statement Ref.3. Source: UniProtKB

   Cellular_componentintegral component of plasma membrane

Non-traceable author statement Ref.3. Source: UniProtKB

   Molecular_functioncarbohydrate binding

Non-traceable author statement Ref.3. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y336-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y336-2)

The sequence of this isoform differs from the canonical sequence as follows:
     402-463: GPLTEPWAED...TEYSEIKIHR → ILNHFIGFPT...FHWFFLICVG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 463446Sialic acid-binding Ig-like lectin 9
PRO_0000014949

Regions

Topological domain18 – 348331Extracellular Potential
Transmembrane349 – 36921Helical; Potential
Topological domain370 – 46394Cytoplasmic Potential
Domain20 – 140121Ig-like V-type
Domain146 – 22984Ig-like C2-type 1
Domain236 – 336101Ig-like C2-type 2
Motif431 – 4366ITIM motif
Motif454 – 4596SLAM-like motif

Sites

Binding site1201Sialic acid By similarity

Amino acid modifications

Glycosylation1011N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation2251N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation2381N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential
Disulfide bond36 ↔ 170 By similarity
Disulfide bond41 ↔ 102 By similarity
Disulfide bond164 ↔ 213 By similarity
Disulfide bond272 ↔ 320 By similarity

Natural variations

Alternative sequence402 – 46362GPLTE…IKIHR → ILNHFIGFPTFLGLGFEFLL NLRDLCCHPDSEFYVYHFSH FRLIKNIAGEIVWSLEGKIL WLLDVSDFFHWFFLICVG in isoform 2.
VSP_054106
Natural variant1001K → E. Ref.8
Corresponds to variant rs2075803 [ dbSNP | Ensembl ].
VAR_014254
Natural variant1251S → N.
Corresponds to variant rs200658 [ dbSNP | Ensembl ].
VAR_014255
Natural variant1311K → Q.
Corresponds to variant rs16988910 [ dbSNP | Ensembl ].
VAR_033621
Natural variant1471N → K.
Corresponds to variant rs273687 [ dbSNP | Ensembl ].
VAR_014256
Natural variant3151A → E. Ref.4 Ref.8
Corresponds to variant rs2258983 [ dbSNP | Ensembl ].
VAR_014257
Natural variant3161A → D.
Corresponds to variant rs273688 [ dbSNP | Ensembl ].
VAR_014258
Natural variant3491V → A.
Corresponds to variant rs273690 [ dbSNP | Ensembl ].
VAR_033622

Experimental info

Mutagenesis1201R → K: Loss of sialic acid binding. Ref.3
Sequence conflict2691R → H in AAF87223. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 2.
Checksum: 807BDCE0D18191F2

FASTA46350,082
        10         20         30         40         50         60 
MLLLLLPLLW GRERAEGQTS KLLTMQSSVT VQEGLCVHVP CSFSYPSHGW IYPGPVVHGY 

        70         80         90        100        110        120 
WFREGANTDQ DAPVATNNPA RAVWEETRDR FHLLGDPHTK NCTLSIRDAR RSDAGRYFFR 

       130        140        150        160        170        180 
MEKGSIKWNY KHHRLSVNVT ALTHRPNILI PGTLESGCPQ NLTCSVPWAC EQGTPPMISW 

       190        200        210        220        230        240 
IGTSVSPLDP STTRSSVLTL IPQPQDHGTS LTCQVTFPGA SVTTNKTVHL NVSYPPQNLT 

       250        260        270        280        290        300 
MTVFQGDGTV STVLGNGSSL SLPEGQSLRL VCAVDAVDSN PPARLSLSWR GLTLCPSQPS 

       310        320        330        340        350        360 
NPGVLELPWV HLRDAAEFTC RAQNPLGSQQ VYLNVSLQSK ATSGVTQGVV GGAGATALVF 

       370        380        390        400        410        420 
LSFCVIFVVV RSCRKKSARP AAGVGDTGIE DANAVRGSAS QGPLTEPWAE DSPPDQPPPA 

       430        440        450        460 
SARSSVGEGE LQYASLSFQM VKPWDSRGQE ATDTEYSEIK IHR 

« Hide

Isoform 2 [UniParc].

Checksum: BA36ABD3025CFC13
Show »

FASTA47952,492

References

« Hide 'large scale' references
[1]"Identification and molecular characterization of a novel member of the siglec family (SIGLEC9)."
Foussias G., Yousef G.M., Diamandis E.P.
Genomics 67:171-178(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
Tissue: Bone marrow.
[2]"Siglec-9, a novel sialic acid binding member of the immunoglobulin superfamily expressed broadly on human blood leukocytes."
Zhang J.Q., Nicoll G., Jones C., Crocker P.R.
J. Biol. Chem. 275:22121-22126(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Cloning, characterization, and phylogenetic analysis of siglec-9, a new member of the CD33-related group of siglecs. Evidence for co-evolution with sialic acid synthesis pathways."
Angata T., Varki A.
J. Biol. Chem. 275:22127-22135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), MUTAGENESIS OF ARG-120.
Tissue: Peripheral blood.
[4]"Molecular cloning of a novel gene, FOAP-9, which are induced by oxydized LDL in human macrophages."
Takayama K., Fujii Y., Turitani K., Naitou K., Kawaguchi A., Ukai Y., Amemiya C., Yajima Y., Yazaki M.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLU-315.
[5]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLU-100 AND GLU-315.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF135027 Genomic DNA. Translation: AAD26428.2.
AF247180 mRNA. Translation: AAF87223.1.
AF227924 mRNA. Translation: AAF71455.1.
AB026265 mRNA. Translation: BAB41100.1.
AY358913 mRNA. Translation: AAQ89272.1.
AK297043 mRNA. Translation: BAG59570.1.
AC011473 Genomic DNA. Translation: AAG23261.1.
BC035365 mRNA. Translation: AAH35365.2.
CCDSCCDS12825.1.
RefSeqNP_001185487.1. NM_001198558.1. [Q9Y336-2]
NP_055256.1. NM_014441.2. [Q9Y336-1]
UniGeneHs.245828.

3D structure databases

ProteinModelPortalQ9Y336.
SMRQ9Y336. Positions 23-233.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000250360.

Protein family/group databases

MEROPSI43.001.

PTM databases

PhosphoSiteQ9Y336.

Polymorphism databases

DMDM25009270.

Proteomic databases

PaxDbQ9Y336.
PRIDEQ9Y336.

Protocols and materials databases

DNASU27180.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000250360; ENSP00000250360; ENSG00000129450.
GeneID27180.
KEGGhsa:27180.
UCSCuc002pvu.3. human. [Q9Y336-1]

Organism-specific databases

CTD27180.
GeneCardsGC19P051628.
HGNCHGNC:10878. SIGLEC9.
HPAHPA010682.
MIM605640. gene.
neXtProtNX_Q9Y336.
PharmGKBPA35779.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG147919.
HOGENOMHOG000236324.
HOVERGENHBG036161.
InParanoidQ9Y336.
KOK06740.
OMAFREKANI.
PhylomeDBQ9Y336.

Gene expression databases

BgeeQ9Y336.
CleanExHS_SIGLEC9.
GenevestigatorQ9Y336.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 2 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSIGLEC9.
GenomeRNAi27180.
NextBio50008.
PROQ9Y336.
SOURCESearch...

Entry information

Entry nameSIGL9_HUMAN
AccessionPrimary (citable) accession number: Q9Y336
Secondary accession number(s): Q6GTU4, Q9BYI9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM