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Protein

Sialic acid-binding Ig-like lectin 9

Gene

SIGLEC9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei120Sialic acidBy similarity1

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processCell adhesion
LigandLectin

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid-binding Ig-like lectin 9
Short name:
Siglec-9
Alternative name(s):
CDw329
Protein FOAP-9
CD_antigen: CD329
Gene namesi
Name:SIGLEC9
ORF Names:UNQ668/PRO1302
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000129450.8.
HGNCiHGNC:10878. SIGLEC9.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 348ExtracellularSequence analysisAdd BLAST331
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Topological domaini370 – 463CytoplasmicSequence analysisAdd BLAST94

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120R → K: Loss of sialic acid binding. 1 Publication1

Organism-specific databases

DisGeNETi27180.
OpenTargetsiENSG00000129450.
PharmGKBiPA35779.

Polymorphism and mutation databases

BioMutaiSIGLEC9.
DMDMi25009270.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001494918 – 463Sialic acid-binding Ig-like lectin 9Add BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 170PROSITE-ProRule annotation
Disulfide bondi41 ↔ 102PROSITE-ProRule annotation
Glycosylationi101N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi138N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi164 ↔ 213PROSITE-ProRule annotation
Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi231N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi238N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi272 ↔ 320PROSITE-ProRule annotation
Glycosylationi334N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ9Y336.
PaxDbiQ9Y336.
PeptideAtlasiQ9Y336.
PRIDEiQ9Y336.

PTM databases

iPTMnetiQ9Y336.
PhosphoSitePlusiQ9Y336.

Expressioni

Tissue specificityi

Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon.

Gene expression databases

BgeeiENSG00000129450.
CleanExiHS_SIGLEC9.
ExpressionAtlasiQ9Y336. baseline and differential.
GenevisibleiQ9Y336. HS.

Organism-specific databases

HPAiHPA010682.

Interactioni

Protein-protein interaction databases

BioGridi118056. 2 interactors.
IntActiQ9Y336. 1 interactor.
STRINGi9606.ENSP00000413861.

Structurei

3D structure databases

ProteinModelPortaliQ9Y336.
SMRiQ9Y336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 140Ig-like V-typeAdd BLAST121
Domaini146 – 229Ig-like C2-type 1Add BLAST84
Domaini236 – 336Ig-like C2-type 2Add BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi431 – 436ITIM motif6
Motifi454 – 459SLAM-like motif6

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJT6. Eukaryota.
ENOG410YKZU. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000236324.
HOVERGENiHBG036161.
InParanoidiQ9Y336.
KOiK06740.
OMAiKTKWNYK.
OrthoDBiEOG091G0445.
PhylomeDBiQ9Y336.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiView protein in InterPro
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
PfamiView protein in Pfam
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 3 hits.
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y336-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLLLPLLW GRERAEGQTS KLLTMQSSVT VQEGLCVHVP CSFSYPSHGW
60 70 80 90 100
IYPGPVVHGY WFREGANTDQ DAPVATNNPA RAVWEETRDR FHLLGDPHTK
110 120 130 140 150
NCTLSIRDAR RSDAGRYFFR MEKGSIKWNY KHHRLSVNVT ALTHRPNILI
160 170 180 190 200
PGTLESGCPQ NLTCSVPWAC EQGTPPMISW IGTSVSPLDP STTRSSVLTL
210 220 230 240 250
IPQPQDHGTS LTCQVTFPGA SVTTNKTVHL NVSYPPQNLT MTVFQGDGTV
260 270 280 290 300
STVLGNGSSL SLPEGQSLRL VCAVDAVDSN PPARLSLSWR GLTLCPSQPS
310 320 330 340 350
NPGVLELPWV HLRDAAEFTC RAQNPLGSQQ VYLNVSLQSK ATSGVTQGVV
360 370 380 390 400
GGAGATALVF LSFCVIFVVV RSCRKKSARP AAGVGDTGIE DANAVRGSAS
410 420 430 440 450
QGPLTEPWAE DSPPDQPPPA SARSSVGEGE LQYASLSFQM VKPWDSRGQE
460
ATDTEYSEIK IHR
Length:463
Mass (Da):50,082
Last modified:May 1, 2000 - v2
Checksum:i807BDCE0D18191F2
GO
Isoform 2 (identifier: Q9Y336-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     402-463: GPLTEPWAED...TEYSEIKIHR → ILNHFIGFPT...FHWFFLICVG

Show »
Length:479
Mass (Da):52,492
Checksum:iBA36ABD3025CFC13
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti269R → H in AAF87223 (PubMed:10801862).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014254100K → E1 PublicationCorresponds to variant dbSNP:rs2075803Ensembl.1
Natural variantiVAR_014255125S → N. Corresponds to variant dbSNP:rs200658Ensembl.1
Natural variantiVAR_033621131K → Q. Corresponds to variant dbSNP:rs16988910Ensembl.1
Natural variantiVAR_014256147N → K. Corresponds to variant dbSNP:rs273687Ensembl.1
Natural variantiVAR_014257315A → E2 PublicationsCorresponds to variant dbSNP:rs2258983Ensembl.1
Natural variantiVAR_014258316A → D. Corresponds to variant dbSNP:rs273688Ensembl.1
Natural variantiVAR_033622349V → A. Corresponds to variant dbSNP:rs273690Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054106402 – 463GPLTE…IKIHR → ILNHFIGFPTFLGLGFEFLL NLRDLCCHPDSEFYVYHFSH FRLIKNIAGEIVWSLEGKIL WLLDVSDFFHWFFLICVG in isoform 2. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135027 Genomic DNA. Translation: AAD26428.2.
AF247180 mRNA. Translation: AAF87223.1.
AF227924 mRNA. Translation: AAF71455.1.
AB026265 mRNA. Translation: BAB41100.1.
AY358913 mRNA. Translation: AAQ89272.1.
AK297043 mRNA. Translation: BAG59570.1.
AC011473 Genomic DNA. Translation: AAG23261.1.
BC035365 mRNA. Translation: AAH35365.2.
CCDSiCCDS12825.1. [Q9Y336-1]
CCDS56100.1. [Q9Y336-2]
RefSeqiNP_001185487.1. NM_001198558.1. [Q9Y336-2]
NP_055256.1. NM_014441.2. [Q9Y336-1]
UniGeneiHs.245828.

Genome annotation databases

EnsembliENST00000250360; ENSP00000250360; ENSG00000129450. [Q9Y336-1]
ENST00000440804; ENSP00000413861; ENSG00000129450. [Q9Y336-2]
GeneIDi27180.
KEGGihsa:27180.
UCSCiuc002pvu.4. human. [Q9Y336-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSIGL9_HUMAN
AccessioniPrimary (citable) accession number: Q9Y336
Secondary accession number(s): Q6GTU4, Q9BYI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families