Q9Y336 (SIGL9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialic acid-binding Ig-like lectin 9
Short name=Siglec-9

Alternative name(s):
CDw329
Protein FOAP-9
CD_antigen=CD329

Gene names

Name:	SIGLEC9
ORF Names:	UNQ668/PRO1302

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon.
Domain	Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Sequence similarities	Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW cell surface receptor signaling pathway Non-traceable author statement Ref.3. Source: UniProtKB
Cellular_component	integral to plasma membrane Non-traceable author statement Ref.3. Source: UniProtKB
Molecular_function	carbohydrate binding Non-traceable author statement Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 463	446	Sialic acid-binding Ig-like lectin 9		PRO_0000014949
Regions
	Topological domain	18 – 348	331	Extracellular Potential
	Transmembrane	349 – 369	21	Helical; Potential
	Topological domain	370 – 463	94	Cytoplasmic Potential
	Domain	20 – 140	121	Ig-like V-type
	Domain	146 – 229	84	Ig-like C2-type 1
	Domain	236 – 336	101	Ig-like C2-type 2
	Motif	431 – 436	6	ITIM motif
	Motif	454 – 459	6	SLAM-like motif
Sites
	Binding site	120	1	Sialic acid By similarity
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Disulfide bond	36 ↔ 170		By similarity
	Disulfide bond	41 ↔ 102		By similarity
	Disulfide bond	164 ↔ 213		By similarity
	Disulfide bond	272 ↔ 320		By similarity
Natural variations
	Natural variant	100	1	K → E. Ref.7 Corresponds to variant rs2075803 [ dbSNP | Ensembl ].		VAR_014254
	Natural variant	125	1	S → N. Corresponds to variant rs200658 [ dbSNP | Ensembl ].		VAR_014255
	Natural variant	131	1	K → Q. Corresponds to variant rs16988910 [ dbSNP | Ensembl ].		VAR_033621
	Natural variant	147	1	N → K. Corresponds to variant rs273687 [ dbSNP | Ensembl ].		VAR_014256
	Natural variant	315	1	A → E. Ref.4 Ref.7 Corresponds to variant rs2258983 [ dbSNP | Ensembl ].		VAR_014257
	Natural variant	316	1	A → D. Corresponds to variant rs273688 [ dbSNP | Ensembl ].		VAR_014258
	Natural variant	349	1	V → A. Corresponds to variant rs273690 [ dbSNP | Ensembl ].		VAR_033622
Experimental info
	Mutagenesis	120	1	R → K: Loss of sialic acid binding. Ref.3
	Sequence conflict	269	1	R → H in AAF87223. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Y336 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: 807BDCE0D18191F2	FASTA	463	50,082
	10 20 30 40 50 60 MLLLLLPLLW GRERAEGQTS KLLTMQSSVT VQEGLCVHVP CSFSYPSHGW IYPGPVVHGY 70 80 90 100 110 120 WFREGANTDQ DAPVATNNPA RAVWEETRDR FHLLGDPHTK NCTLSIRDAR RSDAGRYFFR 130 140 150 160 170 180 MEKGSIKWNY KHHRLSVNVT ALTHRPNILI PGTLESGCPQ NLTCSVPWAC EQGTPPMISW 190 200 210 220 230 240 IGTSVSPLDP STTRSSVLTL IPQPQDHGTS LTCQVTFPGA SVTTNKTVHL NVSYPPQNLT 250 260 270 280 290 300 MTVFQGDGTV STVLGNGSSL SLPEGQSLRL VCAVDAVDSN PPARLSLSWR GLTLCPSQPS 310 320 330 340 350 360 NPGVLELPWV HLRDAAEFTC RAQNPLGSQQ VYLNVSLQSK ATSGVTQGVV GGAGATALVF 370 380 390 400 410 420 LSFCVIFVVV RSCRKKSARP AAGVGDTGIE DANAVRGSAS QGPLTEPWAE DSPPDQPPPA 430 440 450 460 SARSSVGEGE LQYASLSFQM VKPWDSRGQE ATDTEYSEIK IHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and molecular characterization of a novel member of the siglec family (SIGLEC9)." Foussias G., Yousef G.M., Diamandis E.P. Genomics 67:171-178(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Bone marrow.
[2]	"Siglec-9, a novel sialic acid binding member of the immunoglobulin superfamily expressed broadly on human blood leukocytes." Zhang J.Q., Nicoll G., Jones C., Crocker P.R. J. Biol. Chem. 275:22121-22126(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning, characterization, and phylogenetic analysis of siglec-9, a new member of the CD33-related group of siglecs. Evidence for co-evolution with sialic acid synthesis pathways." Angata T., Varki A. J. Biol. Chem. 275:22127-22135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ARG-120. Tissue: Peripheral blood.
[4]	"Molecular cloning of a novel gene, FOAP-9, which are induced by oxydized LDL in human macrophages." Takayama K., Fujii Y., Turitani K., Naitou K., Kawaguchi A., Ukai Y., Amemiya C., Yajima Y., Yazaki M. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-315.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLU-100 AND GLU-315. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Siglec-9

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF135027 Genomic DNA. Translation: AAD26428.2. AF247180 mRNA. Translation: AAF87223.1. AF227924 mRNA. Translation: AAF71455.1. AB026265 mRNA. Translation: BAB41100.1. AY358913 mRNA. Translation: AAQ89272.1. AC011473 Genomic DNA. Translation: AAG23261.1. BC035365 mRNA. Translation: AAH35365.2.
IPI	IPI00004315.
RefSeq	NP_055256.1. NM_014441.2.
UniGene	Hs.245828.
3D structure databases
ProteinModelPortal	Q9Y336.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000250360.
Protein family/group databases
MEROPS	I43.001.
PTM databases
PhosphoSite	Q9Y336.
Polymorphism databases
DMDM	25009270.
Proteomic databases
PaxDb	Q9Y336.
PRIDE	Q9Y336.
Protocols and materials databases
DNASU	27180.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250360; ENSP00000250360; ENSG00000129450.
GeneID	27180.
KEGG	hsa:27180.
UCSC	uc002pvu.3. human.
Organism-specific databases
CTD	27180.
GeneCards	GC19P051628.
HGNC	HGNC:10878. SIGLEC9.
HPA	HPA010682.
MIM	605640. gene.
neXtProt	NX_Q9Y336.
PharmGKB	PA35779.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG147919.
HOGENOM	HOG000236324.
HOVERGEN	HBG036161.
InParanoid	Q9Y336.
KO	K06740.
OMA	TFSWIGV.
OrthoDB	EOG43BMP7.
PhylomeDB	Q9Y336.
Gene expression databases
Bgee	Q9Y336.
CleanEx	HS_SIGLEC9.
Genevestigator	Q9Y336.
GermOnline	ENSG00000129450. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 3 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. IPR013151. Immunoglobulin. [Graphical view]
Pfam	PF00047. ig. 1 hit. PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 3 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	27180.
NextBio	50008.
SOURCE	Search...

Entry information

Entry name

SIGL9_HUMAN

Accession

Primary (citable) accession number: Q9Y336
Secondary accession number(s): Q6GTU4, Q9BYI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.