ID   LSM2_HUMAN              Reviewed;          95 AA.
AC   Q9Y333;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-JAN-2012, entry version 117.
DE   RecName: Full=U6 snRNA-associated Sm-like protein LSm2;
DE   AltName: Full=Protein G7b;
DE   AltName: Full=Small nuclear ribonuclear protein D homolog;
DE   AltName: Full=snRNP core Sm-like protein Sm-x5;
GN   Name=LSM2; Synonyms=C6orf28, G7B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=99452783; PubMed=10523320; DOI=10.1093/emboj/18.20.5789;
RA   Achsel T., Brahms H., Kastner B., Bachi A., Wilm M., Luehrmann R.;
RT   "A doughnut-shaped heteromer of human Sm-like proteins binds to the
RT   3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in
RT   vitro.";
RL   EMBO J. 18:5789-5802(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Olavesen M.G., Campbell R.D.;
RT   "Characterisation of the novel gene G7b located in the class III
RT   region of the human major histocompatibility complex.";
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Schmarda A., Fresser F., Paulmichl M.;
RT   "Human SMX5 homolog.";
RL   Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=14656967; DOI=10.1101/gr.1736803;
RA   Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S.,
RA   Campbell R.D., Hood L.;
RT   "Analysis of the gene-dense major histocompatibility complex class III
RT   region and its comparison to mouse.";
RL   Genome Res. 13:2621-2636(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Shiina S., Tamiya G., Oka A., Inoko H.;
RT   "Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region.";
RL   Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Hypothalamus;
RX   MEDLINE=20402571; PubMed=10931946; DOI=10.1073/pnas.160270997;
RA   Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X.,
RA   Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H.,
RA   Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M., Zhou J.,
RA   Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M.,
RA   Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.;
RT   "Gene expression profiling in the human hypothalamus-pituitary-adrenal
RT   axis and full-length cDNA cloning.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE
RP   SPLICEOSOMAL C COMPLEX.
RX   PubMed=11991638; DOI=10.1017/S1355838202021088;
RA   Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.;
RT   "Purification and characterization of native spliceosomes suitable for
RT   three-dimensional structural analysis.";
RL   RNA 8:426-439(2002).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-35, AND MASS
RP   SPECTROMETRY.
RX   PubMed=15592455; DOI=10.1038/nbt1046;
RA   Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,
RA   Zha X.-M., Polakiewicz R.D., Comb M.J.;
RT   "Immunoaffinity profiling of tyrosine phosphorylation in cancer
RT   cells.";
RL   Nat. Biotechnol. 23:94-101(2005).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-79, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Embryonic kidney;
RX   PubMed=17525332; DOI=10.1126/science.1140321;
RA   Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,
RA   Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,
RA   Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
RT   "ATM and ATR substrate analysis reveals extensive protein networks
RT   responsive to DNA damage.";
RL   Science 316:1160-1166(2007).
RN   [11]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
CC   -!- FUNCTION: Binds specifically to the 3'-terminal U-tract of U6
CC       snRNA. May be involved in pre-mRNA splicing.
CC   -!- SUBUNIT: Identified in the spliceosome C complex. LSm subunits
CC       form a heteromer with a donut shape.
CC   -!- INTERACTION:
CC       O15116:LSM1; NbExp=3; IntAct=EBI-347416, EBI-347619;
CC       P62310:LSM3; NbExp=8; IntAct=EBI-347416, EBI-348239;
CC       Q9UK45:LSM7; NbExp=4; IntAct=EBI-347416, EBI-348372;
CC       O95777:NAA38; NbExp=5; IntAct=EBI-347416, EBI-347779;
CC       Q9H9D4:ZNF408; NbExp=4; IntAct=EBI-347416, EBI-347633;
CC   -!- SUBCELLULAR LOCATION: Nucleus (Potential).
CC   -!- PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
CC   -!- SIMILARITY: Belongs to the snRNP Sm proteins family.
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DR   EMBL; AF182288; AAD56226.1; -; mRNA.
DR   EMBL; AJ245416; CAB52190.1; -; mRNA.
DR   EMBL; AF196468; AAG33023.1; -; mRNA.
DR   EMBL; AF134726; AAD21818.1; -; Genomic_DNA.
DR   EMBL; BA000025; BAB63302.1; -; Genomic_DNA.
DR   EMBL; AF136977; AAG49438.1; -; mRNA.
DR   EMBL; BC009192; AAH09192.1; -; mRNA.
DR   IPI; IPI00032460; -.
DR   RefSeq; NP_067000.1; NM_021177.4.
DR   UniGene; Hs.103106; -.
DR   ProteinModelPortal; Q9Y333; -.
DR   SMR; Q9Y333; 1-71.
DR   DIP; DIP-31139N; -.
DR   IntAct; Q9Y333; 20.
DR   MINT; MINT-1032572; -.
DR   STRING; Q9Y333; -.
DR   PhosphoSite; Q9Y333; -.
DR   DMDM; 10720079; -.
DR   PeptideAtlas; Q9Y333; -.
DR   PRIDE; Q9Y333; -.
DR   Ensembl; ENST00000375661; ENSP00000364813; ENSG00000204392.
DR   Ensembl; ENST00000383391; ENSP00000372883; ENSG00000172850.
DR   Ensembl; ENST00000424975; ENSP00000403345; ENSG00000231502.
DR   Ensembl; ENST00000432122; ENSP00000414006; ENSG00000224979.
DR   Ensembl; ENST00000434125; ENSP00000406280; ENSG00000225998.
DR   Ensembl; ENST00000455705; ENSP00000414634; ENSG00000236826.
DR   GeneID; 57819; -.
DR   KEGG; hsa:57819; -.
DR   UCSC; uc003nxg.1; human.
DR   CTD; 57819; -.
DR   GeneCards; GC06M031765; -.
DR   HGNC; HGNC:13940; LSM2.
DR   MIM; 607282; gene.
DR   neXtProt; NX_Q9Y333; -.
DR   GeneTree; ENSGT00390000016597; -.
DR   HOGENOM; HBG749684; -.
DR   HOVERGEN; HBG000486; -.
DR   InParanoid; Q9Y333; -.
DR   OMA; FIRGSTV; -.
DR   OrthoDB; EOG4Z36G9; -.
DR   PhylomeDB; Q9Y333; -.
DR   Reactome; REACT_111217; Metabolism.
DR   Reactome; REACT_1675; mRNA Processing.
DR   Reactome; REACT_71; Gene Expression.
DR   NextBio; 64772; -.
DR   CleanEx; HS_LSM2; -.
DR   Genevestigator; Q9Y333; -.
DR   GermOnline; ENSG00000204392; Homo sapiens.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0017070; F:U6 snRNA binding; NAS:UniProtKB.
DR   GO; GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; TAS:Reactome.
DR   GO; GO:0000398; P:nuclear mRNA splicing, via spliceosome; NAS:UniProtKB.
DR   InterPro; IPR010920; LSM-related_domain.
DR   InterPro; IPR001163; LSM_domain.
DR   InterPro; IPR006649; LSM_domain_euk/arc.
DR   InterPro; IPR016654; U6_snRNA_Lsm2.
DR   KO; K12621; -.
DR   PANTHER; PTHR13829; PTHR13829; 1.
DR   Pfam; PF01423; LSM; 1.
DR   PIRSF; PIRSF016394; U6_snRNA_Lsm2; 1.
DR   SMART; SM00651; Sm; 1.
DR   SUPFAM; SSF50182; Sm_like_riboprot; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; mRNA processing;
KW   mRNA splicing; Nucleus; Phosphoprotein; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Spliceosome.
FT   CHAIN         1     95       U6 snRNA-associated Sm-like protein LSm2.
FT                                /FTId=PRO_0000125556.
FT   MOD_RES      35     35       Phosphotyrosine.
FT   MOD_RES      79     79       Phosphothreonine.
SQ   SEQUENCE   95 AA;  10835 MW;  623591A09A6ABACE CRC64;
     MLFYSFFKSL VGKDVVVELK NDLSICGTLH SVDQYLNIKL TDISVTDPEK YPHMLSVKNC
     FIRGSVVRYV QLPADEVDTQ LLQDAARKEA LQQKQ
//