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Protein

Nitric oxide synthase-interacting protein

Gene

NOSIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).By similarity3 Publications

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-203754. NOSIP mediated eNOS trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase-interacting protein
Alternative name(s):
E3 ubiquitin-protein ligase NOSIP (EC:6.3.2.-)
eNOS-interacting protein
Gene namesi
Name:NOSIP
ORF Names:CGI-25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:17946. NOSIP.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • Golgi membrane Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134989259.

Polymorphism and mutation databases

DMDMi74735248.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Nitric oxide synthase-interacting proteinPRO_0000280585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361PhosphoserineCombined sources
Modified residuei107 – 1071PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y314.
MaxQBiQ9Y314.
PaxDbiQ9Y314.
PeptideAtlasiQ9Y314.
PRIDEiQ9Y314.

PTM databases

iPTMnetiQ9Y314.
PhosphoSiteiQ9Y314.

Expressioni

Tissue specificityi

Expressed in heart, brain and lung. Present in endothelial cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000142546.
CleanExiHS_NOSIP.
ExpressionAtlasiQ9Y314. baseline and differential.
GenevisibleiQ9Y314. HS.

Organism-specific databases

HPAiHPA043464.
HPA062132.

Interactioni

Subunit structurei

Interacts with NOS1 and NOS3 (PubMed:11149895, PubMed:15548660). Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi119261. 27 interactions.
IntActiQ9Y314. 2 interactions.
MINTiMINT-4832094.
STRINGi9606.ENSP00000375726.

Structurei

3D structure databases

ProteinModelPortaliQ9Y314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 7521U-box-likeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi78 – 10124Nuclear localization signalAdd
BLAST

Domaini

The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.

Sequence similaritiesi

Belongs to the NOSIP family.Curated

Phylogenomic databases

eggNOGiKOG3039. Eukaryota.
ENOG410XQYR. LUCA.
GeneTreeiENSGT00390000015505.
HOGENOMiHOG000007498.
HOVERGENiHBG082046.
InParanoidiQ9Y314.
KOiK13125.
PhylomeDBiQ9Y314.
TreeFamiTF314268.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR016818. Nitric_oxide_synth-interacting.
IPR031790. Znf-NOSIP.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13063:SF10. PTHR13063:SF10. 1 hit.
PfamiPF15906. zf-NOSIP. 1 hit.
[Graphical view]
PIRSFiPIRSF023577. ENOS_interacting. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL
60 70 80 90 100
QPCHDPVVTP DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK
110 120 130 140 150
ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP
160 170 180 190 200
PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV
210 220 230 240 250
HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC
260 270 280 290 300
VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ

A
Length:301
Mass (Da):33,172
Last modified:November 1, 1999 - v1
Checksum:iFB77D6E31511C884
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti168 – 1681T → M.1 Publication
Corresponds to variant rs17850728 [ dbSNP | Ensembl ].
VAR_031169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132959 mRNA. Translation: AAD27734.1.
BC009299 mRNA. Translation: AAH09299.1.
BC010077 mRNA. Translation: AAH10077.1.
BC011249 mRNA. Translation: AAH11249.1.
CCDSiCCDS12772.1.
RefSeqiNP_001257889.1. NM_001270960.1.
NP_057037.1. NM_015953.4.
UniGeneiHs.7236.

Genome annotation databases

EnsembliENST00000596358; ENSP00000470034; ENSG00000142546.
GeneIDi51070.
KEGGihsa:51070.
UCSCiuc002pol.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132959 mRNA. Translation: AAD27734.1.
BC009299 mRNA. Translation: AAH09299.1.
BC010077 mRNA. Translation: AAH10077.1.
BC011249 mRNA. Translation: AAH11249.1.
CCDSiCCDS12772.1.
RefSeqiNP_001257889.1. NM_001270960.1.
NP_057037.1. NM_015953.4.
UniGeneiHs.7236.

3D structure databases

ProteinModelPortaliQ9Y314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119261. 27 interactions.
IntActiQ9Y314. 2 interactions.
MINTiMINT-4832094.
STRINGi9606.ENSP00000375726.

PTM databases

iPTMnetiQ9Y314.
PhosphoSiteiQ9Y314.

Polymorphism and mutation databases

DMDMi74735248.

Proteomic databases

EPDiQ9Y314.
MaxQBiQ9Y314.
PaxDbiQ9Y314.
PeptideAtlasiQ9Y314.
PRIDEiQ9Y314.

Protocols and materials databases

DNASUi51070.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000596358; ENSP00000470034; ENSG00000142546.
GeneIDi51070.
KEGGihsa:51070.
UCSCiuc002pol.5. human.

Organism-specific databases

CTDi51070.
GeneCardsiNOSIP.
HGNCiHGNC:17946. NOSIP.
HPAiHPA043464.
HPA062132.
MIMi616759. gene.
neXtProtiNX_Q9Y314.
PharmGKBiPA134989259.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3039. Eukaryota.
ENOG410XQYR. LUCA.
GeneTreeiENSGT00390000015505.
HOGENOMiHOG000007498.
HOVERGENiHBG082046.
InParanoidiQ9Y314.
KOiK13125.
PhylomeDBiQ9Y314.
TreeFamiTF314268.

Enzyme and pathway databases

ReactomeiR-HSA-203754. NOSIP mediated eNOS trafficking.

Miscellaneous databases

GeneWikiiNOSIP.
GenomeRNAii51070.
PROiQ9Y314.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000142546.
CleanExiHS_NOSIP.
ExpressionAtlasiQ9Y314. baseline and differential.
GenevisibleiQ9Y314. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR016818. Nitric_oxide_synth-interacting.
IPR031790. Znf-NOSIP.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13063:SF10. PTHR13063:SF10. 1 hit.
PfamiPF15906. zf-NOSIP. 1 hit.
[Graphical view]
PIRSFiPIRSF023577. ENOS_interacting. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNOSIP_HUMAN
AccessioniPrimary (citable) accession number: Q9Y314
Secondary accession number(s): Q96FD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.