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Protein

Nitric oxide synthase-interacting protein

Gene

NOSIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).By similarity3 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-203754. NOSIP mediated eNOS trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase-interacting protein
Alternative name(s):
E3 ubiquitin-protein ligase NOSIP (EC:2.3.2.27)
RING-type E3 ubiquitin transferase NOSIPCurated
eNOS-interacting protein
Gene namesi
Name:NOSIP
ORF Names:CGI-25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000142546.13.
HGNCiHGNC:17946. NOSIP.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi51070.
OpenTargetsiENSG00000142546.
PharmGKBiPA134989259.

Polymorphism and mutation databases

DMDMi74735248.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805851 – 301Nitric oxide synthase-interacting proteinAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y314.
MaxQBiQ9Y314.
PaxDbiQ9Y314.
PeptideAtlasiQ9Y314.
PRIDEiQ9Y314.

PTM databases

iPTMnetiQ9Y314.
PhosphoSitePlusiQ9Y314.

Expressioni

Tissue specificityi

Expressed in heart, brain and lung. Present in endothelial cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000142546.
CleanExiHS_NOSIP.
ExpressionAtlasiQ9Y314. baseline and differential.
GenevisibleiQ9Y314. HS.

Organism-specific databases

HPAiHPA043464.
HPA062132.

Interactioni

Subunit structurei

Interacts with NOS1 and NOS3 (PubMed:11149895, PubMed:15548660). Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi119261. 28 interactors.
IntActiQ9Y314. 4 interactors.
MINTiMINT-4832094.
STRINGi9606.ENSP00000375726.

Structurei

3D structure databases

ProteinModelPortaliQ9Y314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 75U-box-likeAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 101Nuclear localization signalAdd BLAST24

Domaini

The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.

Sequence similaritiesi

Belongs to the NOSIP family.Curated

Phylogenomic databases

eggNOGiKOG3039. Eukaryota.
ENOG410XQYR. LUCA.
GeneTreeiENSGT00390000015505.
HOGENOMiHOG000007498.
HOVERGENiHBG082046.
InParanoidiQ9Y314.
KOiK13125.
PhylomeDBiQ9Y314.
TreeFamiTF314268.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR016818. Nitric_oxide_synth-interacting.
IPR031790. Znf-NOSIP.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF15906. zf-NOSIP. 1 hit.
PIRSFiPIRSF023577. ENOS_interacting. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL
60 70 80 90 100
QPCHDPVVTP DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK
110 120 130 140 150
ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP
160 170 180 190 200
PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV
210 220 230 240 250
HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC
260 270 280 290 300
VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ

A
Length:301
Mass (Da):33,172
Last modified:November 1, 1999 - v1
Checksum:iFB77D6E31511C884
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031169168T → M1 PublicationCorresponds to variant dbSNP:rs17850728Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132959 mRNA. Translation: AAD27734.1.
BC009299 mRNA. Translation: AAH09299.1.
BC010077 mRNA. Translation: AAH10077.1.
BC011249 mRNA. Translation: AAH11249.1.
CCDSiCCDS12772.1.
RefSeqiNP_001257889.1. NM_001270960.1.
NP_057037.1. NM_015953.4.
XP_016882340.1. XM_017026851.1.
XP_016882341.1. XM_017026852.1.
UniGeneiHs.7236.

Genome annotation databases

EnsembliENST00000596358; ENSP00000470034; ENSG00000142546.
GeneIDi51070.
KEGGihsa:51070.
UCSCiuc002pol.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNOSIP_HUMAN
AccessioniPrimary (citable) accession number: Q9Y314
Secondary accession number(s): Q96FD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 1, 1999
Last modified: October 25, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families