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Q9Y2Y4 (ZBT32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 32
Alternative name(s):
FANCC-interacting protein
Fanconi anemia zinc finger protein
Testis zinc finger protein
Zinc finger protein 538
Gene names
Name:ZBTB32
Synonyms:FAZF, TZFP, ZNF538
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells By similarity. Ref.2

Subunit structure

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16. Interacts with GATA3 By similarity. Ref.2 Ref.4

Subcellular location

Nucleus. Note: Located in nuclear speckles. Ref.2

Tissue specificity

Predominantly expressed in testis. Some isoforms are ubiquitously expressed. Ref.1

Domain

The C-terminal zinc finger domain functions as a transcriptional transactivator By similarity.

The BTB (POZ) domain possesses repressor activity By similarity.

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 1 BTB (POZ) domain.

Contains 3 C2H2-type zinc fingers.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Hoxa1P090223EBI-954098,EBI-3957603From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487Zinc finger and BTB domain-containing protein 32
PRO_0000273417

Regions

Domain29 – 8759BTB
Zinc finger373 – 39523C2H2-type 1
Zinc finger401 – 42323C2H2-type 2
Zinc finger428 – 45023C2H2-type 3

Natural variations

Natural variant1741R → S.
Corresponds to variant rs2227278 [ dbSNP | Ensembl ].
VAR_030145

Secondary structure

........................... 487
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Y2Y4 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: B528F3C2AFFC4838

FASTA48752,963
        10         20         30         40         50         60 
MSLPPIRLPS PYGSDRLVQL AARLRPALCD TLITVGSQEF PAHSLVLAGV SQQLGRRGQW 

        70         80         90        100        110        120 
ALGEGISPST FAQLLNFVYG ESVELQPGEL RPLQEAARAL GVQSLEEACW RARGDRAKKP 

       130        140        150        160        170        180 
DPGLKKHQEE PEKPSRNPER ELGDPGEKQK PEQVSRTGGR EQEMLHKHSP PRGRPEMAGA 

       190        200        210        220        230        240 
TQEAQQEQTR SKEKRLQAPV GQRGADGKHG VLTWLRENPG GSEESLRKLP GPLPPAGSLQ 

       250        260        270        280        290        300 
TSVTPRPSWA EAPWLVGGQP ALWSILLMPP RYGIPFYHST PTTGAWQEVW REQRIPLSLN 

       310        320        330        340        350        360 
APKGLWSQNQ LASSSPTPGS LPQGPAQLSP GEMEESDQGH TGALATCAGH EDKAGCPPRP 

       370        380        390        400        410        420 
HPPPAPPARS RPYACSVCGK RFSLKHQMET HYRVHTGEKP FSCSLCPQRS RDFSAMTKHL 

       430        440        450        460        470        480 
RTHGAAPYRC SLCGAGCPSL ASMQAHMRGH SPSQLPPGWT IRSTFLYSSS RPSRPSTSPC 


CPSSSTT 

« Hide

References

« Hide 'large scale' references
[1]"Identification and gene structure of a novel human PLZF-related transcription factor gene, TZFP."
Lin W.-C., Lai C.-H., Tang C.J., Huang C.-J., Tang T.K.
Biochem. Biophys. Res. Commun. 264:789-795(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"A novel BTB/POZ transcriptional repressor protein interacts with the Fanconi anemia group C protein and PLZF."
Hoatlin M.E., Zhi Y., Ball H., Silvey K., Melnick A., Stone S., Arai S., Hawe N., Owen G., Zelent A., Licht J.D.
Blood 94:3737-3747(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, INTERACTION WITH ZBTB16 AND FANCC.
Tissue: B-cell.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-294.
Tissue: Lymph.
[4]"Insights into strand exchange in BTB domain dimers from the crystal structures of FAZF and Miz1."
Stogios P.J., Cuesta-Seijo J.A., Chen L., Pomroy N.C., Prive G.G.
J. Mol. Biol. 400:983-997(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-117, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF130255 mRNA. Translation: AAD27708.1.
AF165097 mRNA. Translation: AAD48448.1.
BC017700 mRNA. Translation: AAH17700.1. Different termination.
PIRJC7126.
RefSeqNP_055198.1. NM_014383.1.
XP_005258796.1. XM_005258739.1.
UniGeneHs.99430.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3M5BX-ray2.00A/B1-117[»]
ProteinModelPortalQ9Y2Y4.
SMRQ9Y2Y4. Positions 5-113, 371-451.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117964. 13 interactions.
IntActQ9Y2Y4. 4 interactions.
MINTMINT-1452016.
STRING9606.ENSP00000262630.

PTM databases

PhosphoSiteQ9Y2Y4.

Polymorphism databases

DMDM74762048.

Proteomic databases

PaxDbQ9Y2Y4.
PRIDEQ9Y2Y4.

Protocols and materials databases

DNASU27033.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262630; ENSP00000262630; ENSG00000011590.
ENST00000392197; ENSP00000376035; ENSG00000011590.
ENST00000481182; ENSP00000433657; ENSG00000011590.
GeneID27033.
KEGGhsa:27033.
UCSCuc002oay.3. human.

Organism-specific databases

CTD27033.
GeneCardsGC19P036203.
HGNCHGNC:16763. ZBTB32.
HPAHPA052633.
MIM605859. gene.
neXtProtNX_Q9Y2Y4.
PharmGKBPA142670541.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG296621.
HOGENOMHOG000060112.
HOVERGENHBG079944.
InParanoidQ9Y2Y4.
KOK10506.
OMAGAWQEVW.
OrthoDBEOG7F7W8T.
PhylomeDBQ9Y2Y4.
TreeFamTF350825.

Enzyme and pathway databases

ReactomeREACT_216. DNA Repair.

Gene expression databases

ArrayExpressQ9Y2Y4.
BgeeQ9Y2Y4.
CleanExHS_ZBTB32.
GenevestigatorQ9Y2Y4.

Family and domain databases

Gene3D3.30.160.60. 2 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9Y2Y4.
GeneWikiZBTB32.
GenomeRNAi27033.
NextBio49578.
PROQ9Y2Y4.
SOURCESearch...

Entry information

Entry nameZBT32_HUMAN
AccessionPrimary (citable) accession number: Q9Y2Y4
Secondary accession number(s): Q8WVP2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM