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Protein

DNA-directed RNA polymerase III subunit RPC10

Gene

POLR3K

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 2824C4-typeSequence analysisAdd
BLAST
Zinc fingeri65 – 10743TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA-directed RNA polymerase activity Source: ProtInc
  • nucleic acid binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-HSA-73780. RNA Polymerase III Chain Elongation.
R-HSA-73980. RNA Polymerase III Transcription Termination.
R-HSA-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-HSA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-HSA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-HSA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC10
Short name:
RNA polymerase III subunit C10
Alternative name(s):
DNA-directed RNA polymerase III subunit K
RNA polymerase III 12.5 kDa subunit
Short name:
RPC12.5
RNA polymerase III subunit C11
Short name:
HsC11p
Short name:
RPC11
Short name:
hRPC11
Gene namesi
Name:POLR3K
Synonyms:RPC11
ORF Names:My010
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14121. POLR3K.

Subcellular locationi

  • Nucleusnucleolus By similarity

GO - Cellular componenti

  • cytosol Source: Reactome
  • DNA-directed RNA polymerase III complex Source: ProtInc
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33521.

Polymorphism and mutation databases

BioMutaiPOLR3K.
DMDMi116242768.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 108108DNA-directed RNA polymerase III subunit RPC10PRO_0000121475Add
BLAST

Proteomic databases

EPDiQ9Y2Y1.
MaxQBiQ9Y2Y1.
PaxDbiQ9Y2Y1.
PeptideAtlasiQ9Y2Y1.
PRIDEiQ9Y2Y1.

PTM databases

iPTMnetiQ9Y2Y1.
PhosphoSiteiQ9Y2Y1.

Expressioni

Gene expression databases

BgeeiQ9Y2Y1.
CleanExiHS_POLR3K.
GenevisibleiQ9Y2Y1. HS.

Organism-specific databases

HPAiHPA005891.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

BioGridi119701. 17 interactions.
IntActiQ9Y2Y1. 3 interactions.
STRINGi9606.ENSP00000293860.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2Y1.
SMRiQ9Y2Y1. Positions 68-107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 2824C4-typeSequence analysisAdd
BLAST
Zinc fingeri65 – 10743TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2906. Eukaryota.
COG1594. LUCA.
HOGENOMiHOG000228131.
HOVERGENiHBG055716.
InParanoidiQ9Y2Y1.
KOiK03019.
OrthoDBiEOG741Z4G.
PhylomeDBiQ9Y2Y1.
TreeFamiTF103031.

Family and domain databases

InterProiIPR019761. DNA-dir_RNA_pol-M_15_CS.
IPR001529. DNA-dir_RNA_pol_M/15kDasu.
IPR012164. Rpa12/Rpb9/Rpc10/TFS.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF02150. RNA_POL_M_15KD. 1 hit.
PF01096. TFIIS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005586. RNApol_RpoM. 1 hit.
SMARTiSM00661. RPOL9. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
PROSITEiPS01030. RNA_POL_M_15KD. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y2Y1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLFCPGCGN GLIVEEGQRC HRFSCNTCPY VHNITRKVTN RKYPKLKEVD
60 70 80 90 100
DVLGGAAAWE NVDSTAESCP KCEHPRAYFM QLQTRSADEP MTTFYKCCNA

QCGHRWRD
Length:108
Mass (Da):12,336
Last modified:October 17, 2006 - v2
Checksum:i2C85F2F2AA907CE5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241S → A.5 Publications
Corresponds to variant rs183360 [ dbSNP | Ensembl ].
VAR_027918

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126531 Genomic DNA. Translation: AAD31424.1.
AF051316 mRNA. Translation: AAF18268.1.
AF060223 mRNA. Translation: AAG43123.1.
DQ431198 Genomic DNA. Translation: ABD95903.1.
AE006462 Genomic DNA. Translation: AAK61210.1.
Z69719 Genomic DNA. Translation: CAI95605.1.
BC011932 mRNA. Translation: AAH11932.1.
CCDSiCCDS10395.1.
RefSeqiNP_057394.3. NM_016310.4.
UniGeneiHs.632223.

Genome annotation databases

EnsembliENST00000293860; ENSP00000293860; ENSG00000161980.
GeneIDi51728.
KEGGihsa:51728.
UCSCiuc002cfi.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126531 Genomic DNA. Translation: AAD31424.1.
AF051316 mRNA. Translation: AAF18268.1.
AF060223 mRNA. Translation: AAG43123.1.
DQ431198 Genomic DNA. Translation: ABD95903.1.
AE006462 Genomic DNA. Translation: AAK61210.1.
Z69719 Genomic DNA. Translation: CAI95605.1.
BC011932 mRNA. Translation: AAH11932.1.
CCDSiCCDS10395.1.
RefSeqiNP_057394.3. NM_016310.4.
UniGeneiHs.632223.

3D structure databases

ProteinModelPortaliQ9Y2Y1.
SMRiQ9Y2Y1. Positions 68-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119701. 17 interactions.
IntActiQ9Y2Y1. 3 interactions.
STRINGi9606.ENSP00000293860.

PTM databases

iPTMnetiQ9Y2Y1.
PhosphoSiteiQ9Y2Y1.

Polymorphism and mutation databases

BioMutaiPOLR3K.
DMDMi116242768.

Proteomic databases

EPDiQ9Y2Y1.
MaxQBiQ9Y2Y1.
PaxDbiQ9Y2Y1.
PeptideAtlasiQ9Y2Y1.
PRIDEiQ9Y2Y1.

Protocols and materials databases

DNASUi51728.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000293860; ENSP00000293860; ENSG00000161980.
GeneIDi51728.
KEGGihsa:51728.
UCSCiuc002cfi.3. human.

Organism-specific databases

CTDi51728.
GeneCardsiPOLR3K.
H-InvDBHIX0012635.
HGNCiHGNC:14121. POLR3K.
HPAiHPA005891.
MIMi606007. gene.
neXtProtiNX_Q9Y2Y1.
PharmGKBiPA33521.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2906. Eukaryota.
COG1594. LUCA.
HOGENOMiHOG000228131.
HOVERGENiHBG055716.
InParanoidiQ9Y2Y1.
KOiK03019.
OrthoDBiEOG741Z4G.
PhylomeDBiQ9Y2Y1.
TreeFamiTF103031.

Enzyme and pathway databases

ReactomeiR-HSA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-HSA-73780. RNA Polymerase III Chain Elongation.
R-HSA-73980. RNA Polymerase III Transcription Termination.
R-HSA-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-HSA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-HSA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-HSA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

ChiTaRSiPOLR3K. human.
GeneWikiiPOLR3K.
GenomeRNAii51728.
PROiQ9Y2Y1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y2Y1.
CleanExiHS_POLR3K.
GenevisibleiQ9Y2Y1. HS.

Family and domain databases

InterProiIPR019761. DNA-dir_RNA_pol-M_15_CS.
IPR001529. DNA-dir_RNA_pol_M/15kDasu.
IPR012164. Rpa12/Rpb9/Rpc10/TFS.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF02150. RNA_POL_M_15KD. 1 hit.
PF01096. TFIIS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005586. RNApol_RpoM. 1 hit.
SMARTiSM00661. RPOL9. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
PROSITEiPS01030. RNA_POL_M_15KD. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination."
    Chedin S., Riva M., Schultz P., Sentenac A., Carles C.
    Genes Dev. 12:3857-3871(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-24.
  2. "Molecular identification and characteristics of hRPC11, the smallest specific subunit of human RNA polymerase III."
    Spakovskii G.V., Lebedenko E.N.
    Bioorg. Khim. 24:877-880(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-24.
  3. Mao Y.M., Xie Y., Zheng Z.H., Gu S.H., Ying K., Lin Q., Dai J.L., Tang R., Dong H., Wu X.Z.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-24.
    Tissue: Fetal brain.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-24.
  5. "Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16."
    Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R.
    Hum. Mol. Genet. 10:339-352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-24.
    Tissue: Uterus.
  8. "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits."
    Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N.
    Mol. Cell. Biol. 22:8044-8055(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POL III COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway."
    Chiu Y.-H., Macmillan J.B., Chen Z.J.
    Cell 138:576-591(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate."
    Ablasser A., Bauernfeind F., Hartmann G., Latz E., Fitzgerald K.A., Hornung V.
    Nat. Immunol. 10:1065-1072(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRPC10_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2Y1
Secondary accession number(s): Q1W6H4, Q96S35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 17, 2006
Last modified: July 6, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.