Reviewed, UniProtKB/Swiss-Prot Q9Y2Y0 (AR2BP_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor-like protein 2-binding protein
      Short name=ARF-like 2-binding protein
Alternative name(s):
    Binder of ARF2 protein 1

Gene names

Name:	ARL2BP
Synonyms:	BART, BART1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role as an effector of the ADP-ribosylation factor-like protein 2, ARL2.
Subunit structure	Interacts with GTP bound ARL2 and ARL3; the complex ARL2-ARL2BP as well as ARL2BP alone, binds to ANT1. Ref.1 Ref.5 Ref.6
Subcellular location	Cytoplasm. Mitochondrion intermembrane space. Cytoplasm › cytoskeleton › centrosome.
Tissue specificity	Ubiquitous. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.8
Sequence similarities	Belongs to the ARL2BP family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	signal transduction Ref.1 Traceable author statement. Source: ProtInc
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	small GTPase regulator activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2Y0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2Y0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.
     12-13: SF → MR

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 163

163

ADP-ribosylation factor-like protein 2-binding protein

PRO_0000287113

Amino acid modifications

Modified residue

157

1

Phosphoserine Ref.8

Natural variations

Alternative sequence

1 – 11

11

Missing in isoform 2.

VSP_025317

Alternative sequence

12 – 13

2

SF → MR in isoform 2.

VSP_025318

Natural variant

87

1

E → K: dbSNP rs7198865.

VAR_053904

Experimental info

Sequence conflict

27

1

Y → C in AAH94878. Ref.4

Sequence conflict

50

1

L → Q in AAH94878. Ref.4

Sequence conflict

61

1

I → T in AAH94878. Ref.4

Sequence conflict

83

1

Q → E in AAH94878. Ref.4

Sequence conflict

87

1

E → G in AAH94878. Ref.4

Sequence conflict

154

1

L → T in AAH94878. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

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163

Helix Strand Turn

Details...

Helix

20 – 24

5

Helix

28 – 32

5

Beta strand

33 – 35

3

Helix

36 – 48

13

Helix

49 – 51

3

Beta strand

54 – 57

4

Helix

62 – 84

23

Helix

90 – 97

8

Turn

98 – 100

3

Helix

109 – 114

6

Helix

118 – 132

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: E35EB5AC73FC1FEC	FASTA	163	18,822
	10 20 30 40 50 60 MDALEGESFA LSFSSASDAE FDAVVGYLED IIMDDEFQLL QRNFMDKYYL EFEDTEENKL 70 80 90 100 110 120 IYTPIFNEYI SLVEKYIEEQ LLQRIPEFNM AAFTTTLQHH KDEVAGDIFD MLLTFTDFLA 130 140 150 160 FKEMFLDYRA EKEGRGLDLS SGLVVTSLCK SSSLPASQNN LRH
	Isoform 2. Checksum: 6CC6B4C0A11D35F4	FASTA	152	17,711
	10 20 30 40 50 60 MRSSASDAEF DAVVGYLEDI IMDDEFQLLQ RNFMDKYYLE FEDTEENKLI YTPIFNEYIS 70 80 90 100 110 120 LVEKYIEEQL LQRIPEFNMA AFTTTLQHHK DEVAGDIFDM LLTFTDFLAF KEMFLDYRAE 130 140 150 KEGRGLDLSS GLVVTSLCKS SSLPASQNNL RH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ARF-like 2 (ARL2)-binding protein, BART. Purification, cloning, and initial characterization." Sharer J.D., Kahn R.A. J. Biol. Chem. 274:27553-27561(1999) [PubMed: 10488091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH ARL2, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Mammary gland.
[5]	"ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins." Van Valkenburgh H., Shern J.F., Sharer J.D., Zhu X., Kahn R.A. J. Biol. Chem. 276:22826-22837(2001) [PubMed: 11303027] [Abstract] Cited for: INTERACTION WITH ARL2 AND ARL3.
[6]	"ARL2 and BART enter mitochondria and bind the adenine nucleotide transporter." Sharer J.D., Shern J.F., Van Valkenburgh H., Wallace D.C., Kahn R.A. Mol. Biol. Cell 13:71-83(2002) [PubMed: 11809823] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ANT1.
[7]	"Arl2 and Arl3 regulate different microtubule-dependent processes." Zhou C., Cunningham L., Marcus A.I., Li Y., Kahn R.A. Mol. Biol. Cell 17:2476-2487(2006) [PubMed: 16525022] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF126062 mRNA. Translation: AAD20633.1.
AK075050 mRNA. Translation: BAG52057.1.
CH471092 Genomic DNA. Translation: EAW82913.1.
BC003087 mRNA. Translation: AAH03087.1.
BC094878 mRNA. Translation: AAH94878.1.

IPI

IPI00015866.
IPI00845377.

RefSeq

NP_036238.1.

UniGene

Hs.632873

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2K9A	NMR	-	A	1-136	[»]
3DOE	X-ray	2.25	B	1-163	[»]
3DOF	X-ray	3.30	B	1-163	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q9Y2Y0.

PTM databases

PhosphoSite

Q9Y2Y0.

Proteomic databases

PRIDE

Q9Y2Y0.

Genome annotation databases

Ensembl

ENST00000219204; ENSP00000219204; ENSG00000102931; Homo sapiens. [Genome view]

GeneID

23568.

KEGG

hsa:23568.

UCSC

uc002elf.1. human.

Organism-specific databases

CTD

23568.

GeneCards

GC16P055836.

HGNC

HGNC:17146. ARL2BP.

PharmGKB

PA134904608.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9Y2Y0.

HOVERGEN

Q9Y2Y0.

OMA

CLEDIIM

OrthoDB

EOG9B5RQV

Gene expression databases

ArrayExpress

Q9Y2Y0.

Bgee

Q9Y2Y0.

CleanEx

HS_ARL2BP.

Genevestigator

Q9Y2Y0.

Family and domain databases

ProtoNet

Search...

Other Resources

NextBio

46168.

Entry information

Entry name

AR2BP_HUMAN

Accession

Primary (citable) accession number: Q9Y2Y0
Secondary accession number(s): B3KQJ5, Q504R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	November 1, 1999
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.