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Protein

Zinc finger protein 281

Gene

ZNF281

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.By similarity2 Publications

Caution

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri261 – 283C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri289 – 311C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri317 – 339C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri345 – 367C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • core promoter binding Source: UniProtKB
  • DNA binding transcription factor activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: UniProtKB
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcription corepressor activity Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • embryonic body morphogenesis Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: NTNU_SB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • stem cell differentiation Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processDifferentiation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 281
Alternative name(s):
GC-box-binding zinc finger protein 1
Transcription factor ZBP-99
Zinc finger DNA-binding protein 99
Gene namesi
Name:ZNF281
Synonyms:GZP1, ZBP99
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162702.7
HGNCiHGNC:13075 ZNF281
neXtProtiNX_Q9Y2X9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi23528
OpenTargetsiENSG00000162702
PharmGKBiPA37651

Polymorphism and mutation databases

BioMutaiZNF281
DMDMi13124664

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000475051 – 895Zinc finger protein 281Add BLAST895

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki2Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki101Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki128Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki232Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki242Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki259Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki301Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki325Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki373Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei395PhosphoserineCombined sources1
Cross-linki409Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki416Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki460Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki477Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei484PhosphoserineCombined sources1
Cross-linki493Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki498Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki599Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki617Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki622Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei651PhosphoserineCombined sources1
Cross-linki661Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki670Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei785PhosphoserineCombined sources1
Cross-linki787Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki792Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki795Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei807PhosphoserineCombined sources1
Cross-linki818Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki840Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei888PhosphothreonineCombined sources1
Isoform 2 (identifier: Q9Y2X9-2)
Cross-linki65Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y2X9
PaxDbiQ9Y2X9
PeptideAtlasiQ9Y2X9
PRIDEiQ9Y2X9

PTM databases

iPTMnetiQ9Y2X9
PhosphoSitePlusiQ9Y2X9

Expressioni

Gene expression databases

BgeeiENSG00000162702
CleanExiHS_ZNF281
GenevisibleiQ9Y2X9 HS

Organism-specific databases

HPAiHPA051228

Interactioni

Protein-protein interaction databases

BioGridi11707456 interactors.
CORUMiQ9Y2X9
ELMiQ9Y2X9
IntActiQ9Y2X9 44 interactors.
STRINGi9606.ENSP00000294740

Structurei

3D structure databases

ProteinModelPortaliQ9Y2X9
SMRiQ9Y2X9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 37Gly-richAdd BLAST34
Compositional biasi90 – 96Poly-Pro7
Compositional biasi141 – 148Poly-His8

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri261 – 283C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri289 – 311C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri317 – 339C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri345 – 367C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00530000063238
HOGENOMiHOG000293204
HOVERGENiHBG055321
InParanoidiQ9Y2X9
OMAiQKDIEPR
OrthoDBiEOG091G06OI
PhylomeDBiQ9Y2X9
TreeFamiTF331779

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 4 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2X9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIGSGFLSG GGGTGSSGGS GSGGGGSGGG GGGGSSGRRA EMEPTFPQGM
60 70 80 90 100
VMFNHRLPPV TSFTRPAGSA APPPQCVLSS STSAAPAAEP PPPPAPDMTF
110 120 130 140 150
KKEPAASAAA FPSQRTSWGF LQSLVSIKQE KPADPEEQQS HHHHHHHHYG
160 170 180 190 200
GLFAGAEERS PGLGGGEGGS HGVIQDLSIL HQHVQQQPAQ HHRDVLLSSS
210 220 230 240 250
SRTDDHHGTE EPKQDTNVKK AKRPKPESQG IKAKRKPSAS SKPSLVGDGE
260 270 280 290 300
GAILSPSQKP HICDHCSAAF RSSYHLRRHV LIHTGERPFQ CSQCSMGFIQ
310 320 330 340 350
KYLLQRHEKI HSREKPFGCD QCSMKFIQKY HMERHKRTHS GEKPYKCDTC
360 370 380 390 400
QQYFSRTDRL LKHRRTCGEV IVKGATSAEP GSSNHTNMGN LAVLSQGNTS
410 420 430 440 450
SSRRKTKSKS IAIENKEQKT GKTNESQISN NINMQSYSVE MPTVSSSGGI
460 470 480 490 500
IGTGIDELQK RVPKLIFKKG SRKNTDKNYL NFVSPLPDIV GQKSLSGKPS
510 520 530 540 550
GSLGIVSNNS VETIGLLQST SGKQGQISSN YDDAMQFSKK RRYLPTASSN
560 570 580 590 600
SAFSINVGHM VSQQSVIQSA GVSVLDNEAP LSLIDSSALN AEIKSCHDKS
610 620 630 640 650
GIPDEVLQSI LDQYSNKSES QKEDPFNIAE PRVDLHTSGE HSELVQEENL
660 670 680 690 700
SPGTQTPSND KASMLQEYSK YLQQAFEKST NASFTLGHGF QFVSLSSPLH
710 720 730 740 750
NHTLFPEKQI YTTSPLECGF GQSVTSVLPS SLPKPPFGML FGSQPGLYLS
760 770 780 790 800
ALDATHQQLT PSQELDDLID SQKNLETSSA FQSSSQKLTS QKEQKNLESS
810 820 830 840 850
TGFQIPSQEL ASQIDPQKDI EPRTTYQIEN FAQAFGSQFK SGSRVPMTFI
860 870 880 890
TNSNGEVDHR VRTSVSDFSG YTNMMSDVSE PCSTRVKTPT SQSYR
Length:895
Mass (Da):96,915
Last modified:November 1, 1999 - v1
Checksum:i556FAAEA8D095FB7
GO
Isoform 2 (identifier: Q9Y2X9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     49-84: Missing.

Note: Gene prediction based on EST data.Combined sources
Show »
Length:859
Mass (Da):93,232
Checksum:i180A44DE154DD4FD
GO

Sequence cautioni

The sequence CAB70967 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63F → L in BAG51134 (PubMed:14702039).Curated1
Sequence conflicti206H → Y in AAH51905 (PubMed:15489334).Curated1
Sequence conflicti482F → L in BAG51134 (PubMed:14702039).Curated1
Sequence conflicti635L → I in BAG51134 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035576527I → T in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05481549 – 84Missing in isoform 2. CuratedAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125158 mRNA Translation: AAD21084.1
AJ132591 mRNA Translation: CAB70967.1 Different initiation.
AJ132592 mRNA Translation: CAB70968.1
AK022916 mRNA Translation: BAG51134.1
AC104461 Genomic DNA No translation available.
BC051905 mRNA Translation: AAH51905.1
BC060820 mRNA Translation: AAH60820.1
CCDSiCCDS1402.1 [Q9Y2X9-1]
CCDS60384.1 [Q9Y2X9-2]
PIRiJC7089
RefSeqiNP_001268222.1, NM_001281293.1 [Q9Y2X9-1]
NP_001268223.1, NM_001281294.1 [Q9Y2X9-2]
NP_036614.1, NM_012482.4 [Q9Y2X9-1]
XP_016856376.1, XM_017000887.1 [Q9Y2X9-1]
XP_016856377.1, XM_017000888.1 [Q9Y2X9-1]
UniGeneiHs.59757
Hs.703449
Hs.735801

Genome annotation databases

EnsembliENST00000294740; ENSP00000294740; ENSG00000162702 [Q9Y2X9-1]
ENST00000367352; ENSP00000356321; ENSG00000162702 [Q9Y2X9-2]
ENST00000367353; ENSP00000356322; ENSG00000162702 [Q9Y2X9-1]
GeneIDi23528
KEGGihsa:23528
UCSCiuc001gve.5 human [Q9Y2X9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiZN281_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2X9
Secondary accession number(s): A6NF48
, B3KMX2, Q5RKW5, Q9NY92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome