Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 D4

Gene

UBE2D4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K27-linked ubiquitination Source: UniProtKB
  • protein K29-linked ubiquitination Source: UniProtKB
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein K6-linked ubiquitination Source: UniProtKB
  • protein ubiquitination Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ9Y2X8.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 D4 (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme D4
HBUCE1
Ubiquitin carrier protein D4
Ubiquitin-protein ligase D4
Gene namesi
Name:UBE2D4
Synonyms:UBCH5D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:21647. UBE2D4.

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000078967.
PharmGKBiPA134954568.

Polymorphism and mutation databases

BioMutaiUBE2D4.
DMDMi74753478.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002702021 – 147Ubiquitin-conjugating enzyme E2 D4Add BLAST147

Proteomic databases

EPDiQ9Y2X8.
MaxQBiQ9Y2X8.
PaxDbiQ9Y2X8.
PeptideAtlasiQ9Y2X8.
PRIDEiQ9Y2X8.

PTM databases

iPTMnetiQ9Y2X8.
PhosphoSitePlusiQ9Y2X8.

Expressioni

Gene expression databases

BgeeiENSG00000078967.
CleanExiHS_UBE2D4.
ExpressionAtlasiQ9Y2X8. baseline and differential.
GenevisibleiQ9Y2X8. HS.

Organism-specific databases

HPAiHPA003920.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi119641. 93 interactors.
IntActiQ9Y2X8. 98 interactors.
MINTiMINT-1192345.
STRINGi9606.ENSP00000222402.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2X8.
SMRiQ9Y2X8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ9Y2X8.
KOiK06689.
OMAiICCRTTS.
OrthoDBiEOG091G0GF8.
PhylomeDBiQ9Y2X8.
TreeFamiTF101108.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y2X8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM
Length:147
Mass (Da):16,649
Last modified:November 1, 1999 - v1
Checksum:i5F91A2C6BC667254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125044 mRNA. Translation: AAD31180.1.
AK001446 mRNA. Translation: BAA91697.1.
CH236951 Genomic DNA. Translation: EAL24010.1.
CH471073 Genomic DNA. Translation: EAW94182.1.
BC004104 mRNA. Translation: AAH04104.1.
CCDSiCCDS5474.1.
RefSeqiNP_057067.1. NM_015983.3.
UniGeneiHs.19196.

Genome annotation databases

EnsembliENST00000222402; ENSP00000222402; ENSG00000078967.
GeneIDi51619.
KEGGihsa:51619.
UCSCiuc003tja.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125044 mRNA. Translation: AAD31180.1.
AK001446 mRNA. Translation: BAA91697.1.
CH236951 Genomic DNA. Translation: EAL24010.1.
CH471073 Genomic DNA. Translation: EAW94182.1.
BC004104 mRNA. Translation: AAH04104.1.
CCDSiCCDS5474.1.
RefSeqiNP_057067.1. NM_015983.3.
UniGeneiHs.19196.

3D structure databases

ProteinModelPortaliQ9Y2X8.
SMRiQ9Y2X8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119641. 93 interactors.
IntActiQ9Y2X8. 98 interactors.
MINTiMINT-1192345.
STRINGi9606.ENSP00000222402.

PTM databases

iPTMnetiQ9Y2X8.
PhosphoSitePlusiQ9Y2X8.

Polymorphism and mutation databases

BioMutaiUBE2D4.
DMDMi74753478.

Proteomic databases

EPDiQ9Y2X8.
MaxQBiQ9Y2X8.
PaxDbiQ9Y2X8.
PeptideAtlasiQ9Y2X8.
PRIDEiQ9Y2X8.

Protocols and materials databases

DNASUi51619.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222402; ENSP00000222402; ENSG00000078967.
GeneIDi51619.
KEGGihsa:51619.
UCSCiuc003tja.3. human.

Organism-specific databases

CTDi51619.
GeneCardsiUBE2D4.
HGNCiHGNC:21647. UBE2D4.
HPAiHPA003920.
neXtProtiNX_Q9Y2X8.
OpenTargetsiENSG00000078967.
PharmGKBiPA134954568.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ9Y2X8.
KOiK06689.
OMAiICCRTTS.
OrthoDBiEOG091G0GF8.
PhylomeDBiQ9Y2X8.
TreeFamiTF101108.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ9Y2X8.

Miscellaneous databases

GenomeRNAii51619.
PROiPR:Q9Y2X8.

Gene expression databases

BgeeiENSG00000078967.
CleanExiHS_UBE2D4.
ExpressionAtlasiQ9Y2X8. baseline and differential.
GenevisibleiQ9Y2X8. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUB2D4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2X8
Secondary accession number(s): A4D1V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1999
Last modified: March 15, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.