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Protein

ARF GTPase-activating protein GIT1

Gene

GIT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • ephrin receptor signaling pathway Source: Reactome
  • regulation of cytokinesis Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108262-MONOMER.
ReactomeiR-HSA-3928664. Ephrin signaling.
SignaLinkiQ9Y2X7.
SIGNORiQ9Y2X7.

Names & Taxonomyi

Protein namesi
Recommended name:
ARF GTPase-activating protein GIT1
Short name:
ARF GAP GIT1
Alternative name(s):
Cool-associated and tyrosine-phosphorylated protein 1
Short name:
CAT-1
Short name:
CAT1
G protein-coupled receptor kinase-interactor 1
GRK-interacting protein 1
Gene namesi
Name:GIT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:4272. GIT1.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.

GO - Cellular componenti

  • aggresome Source: HPA
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • focal adhesion Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi28964.
OpenTargetsiENSG00000108262.
PharmGKBiPA28683.

Polymorphism and mutation databases

BioMutaiGIT1.
DMDMi45645212.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742001 – 761ARF GTPase-activating protein GIT1Add BLAST761

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineBy similarity1
Modified residuei359PhosphoserineBy similarity1
Modified residuei362PhosphoserineCombined sources1
Modified residuei364PhosphothreonineCombined sources1
Modified residuei370PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei383PhosphotyrosineCombined sources1
Modified residuei385PhosphoserineCombined sources1
Modified residuei388PhosphoserineCombined sources1
Modified residuei392PhosphothreonineCombined sources1
Modified residuei410PhosphoserineCombined sources1
Modified residuei413PhosphoserineBy similarity1
Modified residuei417PhosphoserineBy similarity1
Modified residuei480PhosphothreonineCombined sources1
Modified residuei498PhosphoserineCombined sources1
Modified residuei536PhosphoserineBy similarity1
Modified residuei537PhosphothreonineBy similarity1
Modified residuei545PhosphotyrosineCombined sources1
Modified residuei554PhosphotyrosineCombined sources1
Modified residuei561PhosphoserineBy similarity1
Modified residuei571PhosphoserineBy similarity1
Modified residuei592PhosphoserineCombined sources1
Modified residuei596PhosphoserineCombined sources1
Modified residuei601PhosphothreonineCombined sources1
Modified residuei630PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y2X7.
PaxDbiQ9Y2X7.
PeptideAtlasiQ9Y2X7.
PRIDEiQ9Y2X7.

PTM databases

iPTMnetiQ9Y2X7.
PhosphoSitePlusiQ9Y2X7.
SwissPalmiQ9Y2X7.

Miscellaneous databases

PMAP-CutDBQ9Y2X7.

Expressioni

Gene expression databases

BgeeiENSG00000108262.
CleanExiHS_GIT1.
ExpressionAtlasiQ9Y2X7. baseline and differential.
GenevisibleiQ9Y2X7. HS.

Organism-specific databases

HPAiHPA004059.
HPA004186.

Interactioni

Subunit structurei

Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4. Interacts with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1 (By similarity). Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1. Interacts with SCRIB. Interacts with SDCCAG3 (PubMed:23108400).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADRBK1P250985EBI-466061,EBI-3904795
ARHGEF7Q141554EBI-466061,EBI-717515
BARD1Q997282EBI-466061,EBI-473181
CEP126Q9P2H02EBI-466061,EBI-473176
CHD3Q128732EBI-466061,EBI-523590
HTTP4285810EBI-466061,EBI-466029
LRIF1Q5T3J32EBI-466061,EBI-473196
PAK1Q131533EBI-466061,EBI-1307
PXNP490243EBI-466061,EBI-2896280From a different organism.

Protein-protein interaction databases

BioGridi118789. 69 interactors.
IntActiQ9Y2X7. 41 interactors.
MINTiMINT-243639.
STRINGi9606.ENSP00000378338.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2X7.
SMRiQ9Y2X7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 124Arf-GAPPROSITE-ProRule annotationAdd BLAST124
Repeati132 – 161ANK 1Add BLAST30
Repeati166 – 195ANK 2Add BLAST30
Repeati199 – 228ANK 3Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 415PTK2/FAK1-bindingBy similarityAdd BLAST165
Regioni254 – 367ARHGEF6-bindingBy similarityAdd BLAST114
Regioni637 – 761Interaction with PXN and TGFB1I1By similarityAdd BLAST125

Domaini

The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.By similarity

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0818. Eukaryota.
ENOG410XR8U. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000232135.
HOVERGENiHBG012506.
InParanoidiQ9Y2X7.
KOiK05737.
OMAiQKCMSQS.
OrthoDBiEOG091G03VD.
PhylomeDBiQ9Y2X7.
TreeFamiTF317762.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR032352. GIT1/2_CC.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF16559. GIT_CC. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2X7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH
60 70 80 90 100
LRHSAWPPTL LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH
110 120 130 140 150
PIKSEFIRAK YQMLAFVHKL PCRDDDGVTA KDLSKQLHSS VRTGNLETCL
160 170 180 190 200
RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT LQAELLVVYG ADPGSPDVNG
210 220 230 240 250
RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD HKNGHYIIPQ
260 270 280 290 300
MADSLDLSEL AKAAKKKLQA LSNRLFEELA MDVYDEVDRR ENDAVWLATQ
310 320 330 340 350
NHSTLVTERS AVPFLPVNPE YSATRNQGRQ KLARFNAREF ATLIIDILSE
360 370 380 390 400
AKRRQQGKSL SSPTDNLELS LRSQSDLDDQ HDYDSVASDE DTDQEPLRST
410 420 430 440 450
GATRSNRARS MDSSDLSDGA VTLQEYLELK KALATSEAKV QQLMKVNSSL
460 470 480 490 500
SDELRRLQRE IHKLQAENLQ LRQPPGPVPT PPLPSERAEH TPMAPGGSTH
510 520 530 540 550
RRDRQAFSMY EPGSALKPFG GPPGDELTTR LQPFHSTELE DDAIYSVHVP
560 570 580 590 600
AGLYRIRKGV SASAVPFTPS SPLLSCSQEG SRHTSKLSRH GSGADSDYEN
610 620 630 640 650
TQSGDPLLGL EGKRFLELGK EEDFHPELES LDGDLDPGLP STEDVILKTE
660 670 680 690 700
QVTKNIQELL RAAQEFKHDS FVPCSEKIHL AVTEMASLFP KRPALEPVRS
710 720 730 740 750
SLRLLNASAY RLQSECRKTV PPEPGAPVDF QLLTQQVIQC AYDIAKAAKQ
760
LVTITTREKK Q
Length:761
Mass (Da):84,341
Last modified:March 15, 2004 - v2
Checksum:i702744942A796399
GO
Isoform 2 (identifier: Q9Y2X7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-176: SKQLHSSVRT...GTTPLHVAAK → TGHRSWATPD...VPWCPPLSSP
     177-761: Missing.

Note: No experimental confirmation available.
Show »
Length:176
Mass (Da):19,160
Checksum:i2B5BB45555AB5201
GO
Isoform 3 (identifier: Q9Y2X7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     253-253: D → DRSRQKCMSQ

Show »
Length:770
Mass (Da):85,446
Checksum:i278AD8D706C32EE0
GO

Sequence cautioni

The sequence AAH48196 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121P → S in AAD28046 (PubMed:10896954).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009666134 – 176SKQLH…HVAAK → TGHRSWATPDINPHPNRATG TSALSAATLPGSQVPWCPPL SSP in isoform 2. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_009667177 – 761Missing in isoform 2. 1 PublicationAdd BLAST585
Alternative sequenceiVSP_040984253D → DRSRQKCMSQ in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124490 mRNA. Translation: AAD28046.1.
AK294785 mRNA. Translation: BAG57910.1.
AK299932 mRNA. Translation: BAG61765.1.
AC104564 Genomic DNA. No translation available.
BC006227 mRNA. Translation: AAH06227.2.
BC048196 mRNA. Translation: AAH48196.1. Different initiation.
CCDSiCCDS11250.1. [Q9Y2X7-1]
CCDS42290.1. [Q9Y2X7-3]
RefSeqiNP_001078923.1. NM_001085454.1. [Q9Y2X7-3]
NP_054749.2. NM_014030.3. [Q9Y2X7-1]
UniGeneiHs.514051.

Genome annotation databases

EnsembliENST00000225394; ENSP00000225394; ENSG00000108262. [Q9Y2X7-1]
ENST00000394869; ENSP00000378338; ENSG00000108262. [Q9Y2X7-3]
GeneIDi28964.
KEGGihsa:28964.
UCSCiuc002hef.3. human. [Q9Y2X7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124490 mRNA. Translation: AAD28046.1.
AK294785 mRNA. Translation: BAG57910.1.
AK299932 mRNA. Translation: BAG61765.1.
AC104564 Genomic DNA. No translation available.
BC006227 mRNA. Translation: AAH06227.2.
BC048196 mRNA. Translation: AAH48196.1. Different initiation.
CCDSiCCDS11250.1. [Q9Y2X7-1]
CCDS42290.1. [Q9Y2X7-3]
RefSeqiNP_001078923.1. NM_001085454.1. [Q9Y2X7-3]
NP_054749.2. NM_014030.3. [Q9Y2X7-1]
UniGeneiHs.514051.

3D structure databases

ProteinModelPortaliQ9Y2X7.
SMRiQ9Y2X7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118789. 69 interactors.
IntActiQ9Y2X7. 41 interactors.
MINTiMINT-243639.
STRINGi9606.ENSP00000378338.

PTM databases

iPTMnetiQ9Y2X7.
PhosphoSitePlusiQ9Y2X7.
SwissPalmiQ9Y2X7.

Polymorphism and mutation databases

BioMutaiGIT1.
DMDMi45645212.

Proteomic databases

EPDiQ9Y2X7.
PaxDbiQ9Y2X7.
PeptideAtlasiQ9Y2X7.
PRIDEiQ9Y2X7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225394; ENSP00000225394; ENSG00000108262. [Q9Y2X7-1]
ENST00000394869; ENSP00000378338; ENSG00000108262. [Q9Y2X7-3]
GeneIDi28964.
KEGGihsa:28964.
UCSCiuc002hef.3. human. [Q9Y2X7-1]

Organism-specific databases

CTDi28964.
DisGeNETi28964.
GeneCardsiGIT1.
H-InvDBHIX0013673.
HGNCiHGNC:4272. GIT1.
HPAiHPA004059.
HPA004186.
MIMi608434. gene.
neXtProtiNX_Q9Y2X7.
OpenTargetsiENSG00000108262.
PharmGKBiPA28683.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0818. Eukaryota.
ENOG410XR8U. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000232135.
HOVERGENiHBG012506.
InParanoidiQ9Y2X7.
KOiK05737.
OMAiQKCMSQS.
OrthoDBiEOG091G03VD.
PhylomeDBiQ9Y2X7.
TreeFamiTF317762.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108262-MONOMER.
ReactomeiR-HSA-3928664. Ephrin signaling.
SignaLinkiQ9Y2X7.
SIGNORiQ9Y2X7.

Miscellaneous databases

ChiTaRSiGIT1. human.
GeneWikiiGIT1.
GenomeRNAii28964.
PMAP-CutDBQ9Y2X7.
PROiQ9Y2X7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108262.
CleanExiHS_GIT1.
ExpressionAtlasiQ9Y2X7. baseline and differential.
GenevisibleiQ9Y2X7. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR032352. GIT1/2_CC.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF16559. GIT_CC. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGIT1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2X7
Secondary accession number(s): B4DGU9
, B4DSV3, Q86SS0, Q9BRJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 15, 2004
Last modified: November 30, 2016
This is version 174 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.