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Protein

Nucleolar protein 58

Gene

NOP58

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for 60S ribosomal subunit biogenesis (By similarity). Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs.By similarity3 Publications

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • snoRNA binding Source: BHF-UCL
  • TFIID-class transcription factor binding Source: UniProtKB

GO - Biological processi

  • cell growth Source: UniProtKB
  • protein sumoylation Source: Reactome
  • rRNA modification Source: GO_Central
  • rRNA processing Source: UniProtKB
  • snoRNA localization Source: UniProtKB
  • snRNP protein import into nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000055044-MONOMER.
ReactomeiR-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-6790901. rRNA modification in the nucleus and cytosol.
R-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 58
Alternative name(s):
Nucleolar protein 5
Gene namesi
Name:NOP58
Synonyms:NOL5, NOP5
ORF Names:HSPC120
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:29926. NOP58.

Subcellular locationi

GO - Cellular componenti

  • box C/D snoRNP complex Source: BHF-UCL
  • Cajal body Source: BHF-UCL
  • cytoplasm Source: HPA
  • membrane Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
  • pre-snoRNP complex Source: BHF-UCL
  • small nucleolar ribonucleoprotein complex Source: BHF-UCL
  • small-subunit processome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000055044.
PharmGKBiPA164724092.

Polymorphism and mutation databases

BioMutaiNOP58.
DMDMi17380155.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190231 – 529Nucleolar protein 58Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphothreonineCombined sources1
Modified residuei109PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Cross-linki415Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki465Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linki467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei483PhosphoserineCombined sources1
Cross-linki497Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linki497Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei502PhosphoserineCombined sources1
Modified residuei514PhosphoserineCombined sources1

Post-translational modificationi

Sumoylation is essential for high-affinity binding to snoRNAs.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y2X3.
MaxQBiQ9Y2X3.
PaxDbiQ9Y2X3.
PeptideAtlasiQ9Y2X3.
PRIDEiQ9Y2X3.

2D gel databases

SWISS-2DPAGEQ9Y2X3.

PTM databases

iPTMnetiQ9Y2X3.
PhosphoSitePlusiQ9Y2X3.
SwissPalmiQ9Y2X3.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000055044.
ExpressionAtlasiQ9Y2X3. baseline and differential.
GenevisibleiQ9Y2X3. HS.

Organism-specific databases

HPAiHPA018472.
HPA021062.

Interactioni

Subunit structurei

Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL assemble stepwise onto the snoRNA. Interacts with NOLC1/Nopp140. Interacts with NUFIP1, RUVBL1 AND RUVBL2; RUVBL1:RUVBL2 seem to bridge the association of NOP58 with NUFIP1. Interacts with PIH1D1 (PubMed:17636026).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TGS1Q96RS02EBI-395469,EBI-949244

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • TFIID-class transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119631. 113 interactors.
DIPiDIP-32926N.
IntActiQ9Y2X3. 40 interactors.
MINTiMINT-3085086.
STRINGi9606.ENSP00000264279.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2X3.
SMRiQ9Y2X3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini282 – 400NopPROSITE-ProRule annotationAdd BLAST119

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi441 – 524Lys-richAdd BLAST84

Sequence similaritiesi

Belongs to the NOP5/NOP56 family.Curated
Contains 1 Nop domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2572. Eukaryota.
COG1498. LUCA.
GeneTreeiENSGT00550000074998.
HOGENOMiHOG000196310.
InParanoidiQ9Y2X3.
KOiK14565.
OMAiGLAHXLL.
OrthoDBiEOG091G07GD.
PhylomeDBiQ9Y2X3.
TreeFamiTF105688.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y2X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVLFETSVG YAIFKVLNEK KLQEVDSLWK EFETPEKANK IVKLKHFEKF
60 70 80 90 100
QDTAEALAAF TALMEGKINK QLKKVLKKIV KEAHEPLAVA DAKLGGVIKE
110 120 130 140 150
KLNLSCIHSP VVNELMRGIR SQMDGLIPGV EPREMAAMCL GLAHSLSRYR
160 170 180 190 200
LKFSADKVDT MIVQAISLLD DLDKELNNYI MRCREWYGWH FPELGKIISD
210 220 230 240 250
NLTYCKCLQK VGDRKNYASA KLSELLPEEV EAEVKAAAEI SMGTEVSEED
260 270 280 290 300
ICNILHLCTQ VIEISEYRTQ LYEYLQNRMM AIAPNVTVMV GELVGARLIA
310 320 330 340 350
HAGSLLNLAK HAASTVQILG AEKALFRALK SRRDTPKYGL IYHASLVGQT
360 370 380 390 400
SPKHKGKISR MLAAKTVLAI RYDAFGEDSS SAMGVENRAK LEARLRTLED
410 420 430 440 450
RGIRKISGTG KALAKTEKYE HKSEVKTYDP SGDSTLPTCS KKRKIEQVDK
460 470 480 490 500
EDEITEKKAK KAKIKVKVEE EEEEKVAEEE ETSVKKKKKR GKKKHIKEEP
510 520
LSEEEPCTST AIASPEKKKK KKKKRENED
Length:529
Mass (Da):59,578
Last modified:November 1, 1999 - v1
Checksum:i27CD73CFF5B9A556
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2L → M in CAB55989 (PubMed:17974005).Curated1
Sequence conflicti129G → V in CAB55989 (PubMed:17974005).Curated1
Sequence conflicti202 – 221LTYCK…YASAK → YHTASVYRKLAIGRLCLCQ in AAF29084 (PubMed:11042152).CuratedAdd BLAST20
Sequence conflicti235 – 260KAAAE…HLCTQ → EGSCRDIHGNRGFRRRYLQY SASLHP in AAF29084 (PubMed:11042152).CuratedAdd BLAST26
Sequence conflicti280M → V in CAB55989 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059461387N → T.Corresponds to variant rs34748654dbSNPEnsembl.1
Natural variantiVAR_059462389A → P.Corresponds to variant rs34458926dbSNPEnsembl.1
Natural variantiVAR_059463400D → A.Corresponds to variant rs35900977dbSNPEnsembl.1
Natural variantiVAR_059464508T → P.Corresponds to variant rs34523815dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123534 mRNA. Translation: AAD27610.1.
AF263608 mRNA. Translation: AAF91394.1.
AC064836 Genomic DNA. Translation: AAY24145.1.
BC032592 mRNA. Translation: AAH32592.1.
BC009306 mRNA. Translation: AAH09306.1.
AL117554 mRNA. Translation: CAB55989.2.
AF161469 mRNA. Translation: AAF29084.1.
CCDSiCCDS2353.1.
PIRiT17299.
RefSeqiNP_057018.1. NM_015934.4.
UniGeneiHs.471104.

Genome annotation databases

EnsembliENST00000264279; ENSP00000264279; ENSG00000055044.
GeneIDi51602.
KEGGihsa:51602.
UCSCiuc002uzb.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123534 mRNA. Translation: AAD27610.1.
AF263608 mRNA. Translation: AAF91394.1.
AC064836 Genomic DNA. Translation: AAY24145.1.
BC032592 mRNA. Translation: AAH32592.1.
BC009306 mRNA. Translation: AAH09306.1.
AL117554 mRNA. Translation: CAB55989.2.
AF161469 mRNA. Translation: AAF29084.1.
CCDSiCCDS2353.1.
PIRiT17299.
RefSeqiNP_057018.1. NM_015934.4.
UniGeneiHs.471104.

3D structure databases

ProteinModelPortaliQ9Y2X3.
SMRiQ9Y2X3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119631. 113 interactors.
DIPiDIP-32926N.
IntActiQ9Y2X3. 40 interactors.
MINTiMINT-3085086.
STRINGi9606.ENSP00000264279.

PTM databases

iPTMnetiQ9Y2X3.
PhosphoSitePlusiQ9Y2X3.
SwissPalmiQ9Y2X3.

Polymorphism and mutation databases

BioMutaiNOP58.
DMDMi17380155.

2D gel databases

SWISS-2DPAGEQ9Y2X3.

Proteomic databases

EPDiQ9Y2X3.
MaxQBiQ9Y2X3.
PaxDbiQ9Y2X3.
PeptideAtlasiQ9Y2X3.
PRIDEiQ9Y2X3.

Protocols and materials databases

DNASUi51602.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264279; ENSP00000264279; ENSG00000055044.
GeneIDi51602.
KEGGihsa:51602.
UCSCiuc002uzb.4. human.

Organism-specific databases

CTDi51602.
GeneCardsiNOP58.
HGNCiHGNC:29926. NOP58.
HPAiHPA018472.
HPA021062.
MIMi616742. gene.
neXtProtiNX_Q9Y2X3.
OpenTargetsiENSG00000055044.
PharmGKBiPA164724092.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2572. Eukaryota.
COG1498. LUCA.
GeneTreeiENSGT00550000074998.
HOGENOMiHOG000196310.
InParanoidiQ9Y2X3.
KOiK14565.
OMAiGLAHXLL.
OrthoDBiEOG091G07GD.
PhylomeDBiQ9Y2X3.
TreeFamiTF105688.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000055044-MONOMER.
ReactomeiR-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-6790901. rRNA modification in the nucleus and cytosol.
R-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

ChiTaRSiNOP58. human.
GeneWikiiNOP5/NOP58.
GenomeRNAii51602.
PROiQ9Y2X3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000055044.
ExpressionAtlasiQ9Y2X3. baseline and differential.
GenevisibleiQ9Y2X3. HS.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOP58_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2X3
Secondary accession number(s): Q53SA4
, Q6PK08, Q9P036, Q9UFN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.