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Q9Y2X0

- MED16_HUMAN

UniProt

Q9Y2X0 - MED16_HUMAN

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Protein

Mediator of RNA polymerase II transcription subunit 16

Gene
MED16, DRIP92, THRAP5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

  1. catalytic activity Source: InterPro
  2. receptor activity Source: UniProtKB
  3. thyroid hormone receptor binding Source: UniProtKB
  4. thyroid hormone receptor coactivator activity Source: UniProtKB
  5. transcription coactivator activity Source: ProtInc
  6. transcription cofactor activity Source: UniProtKB
  7. vitamin D receptor binding Source: UniProtKB

GO - Biological processi

  1. androgen receptor signaling pathway Source: UniProtKB
  2. gene expression Source: Reactome
  3. intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  4. positive regulation of receptor activity Source: GOC
  5. positive regulation of transcription, DNA-templated Source: UniProtKB
  6. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  7. transcription from RNA polymerase II promoter Source: ProtInc
  8. transcription initiation from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.
SignaLinkiQ9Y2X0.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 16
Alternative name(s):
Mediator complex subunit 16
Thyroid hormone receptor-associated protein 5
Thyroid hormone receptor-associated protein complex 95 kDa component
Short name:
Trap95
Vitamin D3 receptor-interacting protein complex 92 kDa component
Short name:
DRIP92
Gene namesi
Name:MED16
Synonyms:DRIP92, THRAP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:17556. MED16.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. mediator complex Source: UniProtKB
  2. nucleoplasm Source: Reactome
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395406.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877Mediator of RNA polymerase II transcription subunit 16PRO_0000051292Add
BLAST

Proteomic databases

MaxQBiQ9Y2X0.
PaxDbiQ9Y2X0.
PRIDEiQ9Y2X0.

PTM databases

PhosphoSiteiQ9Y2X0.

Expressioni

Gene expression databases

ArrayExpressiQ9Y2X0.
BgeeiQ9Y2X0.
CleanExiHS_MED16.
GenevestigatoriQ9Y2X0.

Organism-specific databases

HPAiHPA030685.
HPA046495.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.4 Publications

Protein-protein interaction databases

BioGridi115342. 46 interactions.
DIPiDIP-31463N.
IntActiQ9Y2X0. 12 interactions.
MINTiMINT-6744281.
STRINGi9606.ENSP00000325612.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2X0.
SMRiQ9Y2X0. Positions 71-113.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati79 – 11840WD 1Add
BLAST
Repeati210 – 25243WD 2Add
BLAST
Repeati275 – 31945WD 3Add
BLAST
Repeati634 – 67542WD 4Add
BLAST
Repeati790 – 82940WD 5Add
BLAST

Sequence similaritiesi

Contains 5 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiNOG85731.
HOGENOMiHOG000113526.
HOVERGENiHBG054317.
InParanoidiQ9Y2X0.
KOiK15159.
OMAiMRCTTDP.
OrthoDBiEOG7NCV3M.
PhylomeDBiQ9Y2X0.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR021665. Mediator_Med16.
IPR011041. Quinoprot_gluc/sorb_DH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11635. Med16. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 1 hit.
[Graphical view]
SUPFAMiSSF50952. SSF50952. 4 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q9Y2X0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD    50
LRSDDQDLTR MIHILDTEHP WDLHSIPSEH HEAITCLEWD QSGSRLLSAD 100
ADGQIKCWSM ADHLANSWES SVGSLVEGDP IVALSWLHNG VKLALHVEKS 150
GASSFGEKFS RVKFSPSLTL FGGKPMEGWI AVTVSGLVTV SLLKPSGQVL 200
TSTESLCRLR GRVALADIAF TGGGNIVVAT ADGSSASPVQ FYKVCVSVVS 250
EKCRIDTEIL PSLFMRCTTD LNRKDKFPAI THLKFLARDM SEQVLLCASS 300
QTSSIVECWS LRKEGLPVNN IFQQISPVVG DKQPTILKWR ILSATNDLDR 350
VSAVALPKLP ISLTNTDLKV ASDTQFYPGL GLALAFHDGS VHIVHRLSLQ 400
TMAVFYSSAA PRPVDEPAMK RPRTAGPAVH LKAMQLSWTS LALVGIDSHG 450
KLSVLRLSPS MGHPLEVGLA LRHLLFLLEY CMVTGYDWWD ILLHVQPSMV 500
QSLVEKLHEE YTRQTAALQQ VLSTRILAMK ASLCKLSPCT VTRVCDYHTK 550
LFLIAISSTL KSLLRPHFLN TPDKSPGDRL TEICTKITDV DIDKVMINLK 600
TEEFVLDMNT LQALQQLLQW VGDFVLYLLA SLPNQGSLLR PGHSFLRDGT 650
SLGMLRELMV VIRIWGLLKP SCLPVYTATS DTQDSMSLLF RLLTKLWICC 700
RDEGPASEPD EALVDECCLL PSQLLIPSLD WLPASDGLVS RLQPKQPLRL 750
QFGRAPTLPG SAATLQLDGL ARAPGQPKID HLRRLHLGAC PTEECKACTR 800
CGCVTMLKSP NRTTAVKQWE QRWIKNCLAV EGRGPDACVT SRASEEAPAF 850
VQLGPQSTHH SPRTPRSLDH LHPEDRP 877
Length:877
Mass (Da):96,793
Last modified:April 12, 2005 - v2
Checksum:i15173F237A75D230
GO
Isoform 2 (identifier: Q9Y2X0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     829-877: AVEGRGPDACVTSRASEEAPAFVQLGPQSTHHSPRTPRSLDHLHPEDRP → CGGLWWRVPLSYP

Show »
Length:841
Mass (Da):93,029
Checksum:i2D9CAF68001320C3
GO
Isoform 3 (identifier: Q9Y2X0-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     700-700: C → FPSTGPCSVWVLLGWQPLPG
     829-877: AVEGRGPDACVTSRASEEAPAFVQLGPQSTHHSPRTPRSLDHLHPEDRP → CGGLWWRVPLSYP

Note: No experimental confirmation available.

Show »
Length:860
Mass (Da):95,050
Checksum:i546EA25BE3AB56CF
GO
Isoform 4 (identifier: Q9Y2X0-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     636-751: GSLLRPGHSF...LQPKQPLRLQ → VAMRAQRASR...CSSRPTEPRR
     752-877: Missing.

Note: No experimental confirmation available.

Show »
Length:751
Mass (Da):82,179
Checksum:i6B77C11B864902BD
GO
Isoform 5 (identifier: Q9Y2X0-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     636-663: GSLLRPGHSFLRDGTSLGMLRELMVVIR → PCPTSEPCPTSEPSPTSEPSPTSEPSSP
     664-877: Missing.

Note: No experimental confirmation available.

Show »
Length:663
Mass (Da):72,967
Checksum:i311F7B98C3912283
GO

Sequence cautioni

The sequence AAD31087.1 differs from that shown. Reason: Frameshift at position 609.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti770 – 7701L → F.
Corresponds to variant rs34859566 [ dbSNP | Ensembl ].
VAR_053958
Natural varianti874 – 8741E → K.
Corresponds to variant rs13090 [ dbSNP | Ensembl ].
VAR_053959

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei636 – 751116GSLLR…PLRLQ → VAMRAQRASRTRRWWMNAAC CPASCLSPAWTGCQPATAWL AACSPSSPFVCSLAGRPRCL AVLPPCSSTASPGPQASPRS TTCGGCTLALAPRRNARPAP GAAVSPCSSRPTEPRR in isoform 4. VSP_051721Add
BLAST
Alternative sequencei636 – 66328GSLLR…MVVIR → PCPTSEPCPTSEPSPTSEPS PTSEPSSP in isoform 5. VSP_028749Add
BLAST
Alternative sequencei664 – 877214Missing in isoform 5. VSP_028750Add
BLAST
Alternative sequencei700 – 7001C → FPSTGPCSVWVLLGWQPLPG in isoform 3. VSP_051722
Alternative sequencei752 – 877126Missing in isoform 4. VSP_051723Add
BLAST
Alternative sequencei829 – 87749AVEGR…PEDRP → CGGLWWRVPLSYP in isoform 2 and isoform 3. 1 PublicationVSP_051724Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461A → T in AAD30032. 1 Publication
Sequence conflicti211 – 2111G → A in AAD31087. 1 Publication
Sequence conflicti412 – 4121R → K in AAD30032. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF121228 mRNA. Translation: AAD30032.1.
AF106934 mRNA. Translation: AAD31087.1. Frameshift.
BC004554 mRNA. Translation: AAH04554.1.
BC007853 mRNA. Translation: AAH07853.2.
BC011841 mRNA. Translation: AAH11841.1.
BC017282 mRNA. Translation: AAH17282.1.
CCDSiCCDS12047.1. [Q9Y2X0-1]
RefSeqiNP_005472.2. NM_005481.2. [Q9Y2X0-1]
XP_006722663.1. XM_006722600.1. [Q9Y2X0-1]
UniGeneiHs.365207.

Genome annotation databases

EnsembliENST00000269814; ENSP00000269814; ENSG00000175221. [Q9Y2X0-4]
ENST00000312090; ENSP00000308528; ENSG00000175221. [Q9Y2X0-3]
ENST00000325464; ENSP00000325612; ENSG00000175221. [Q9Y2X0-1]
ENST00000395808; ENSP00000379153; ENSG00000175221. [Q9Y2X0-2]
ENST00000589119; ENSP00000464810; ENSG00000175221. [Q9Y2X0-1]
GeneIDi10025.
KEGGihsa:10025.
UCSCiuc002lqd.1. human. [Q9Y2X0-1]
uc002lqe.3. human. [Q9Y2X0-2]
uc002lqf.3. human. [Q9Y2X0-3]
uc010drw.2. human. [Q9Y2X0-4]

Polymorphism databases

DMDMi62511180.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF121228 mRNA. Translation: AAD30032.1 .
AF106934 mRNA. Translation: AAD31087.1 . Frameshift.
BC004554 mRNA. Translation: AAH04554.1 .
BC007853 mRNA. Translation: AAH07853.2 .
BC011841 mRNA. Translation: AAH11841.1 .
BC017282 mRNA. Translation: AAH17282.1 .
CCDSi CCDS12047.1. [Q9Y2X0-1 ]
RefSeqi NP_005472.2. NM_005481.2. [Q9Y2X0-1 ]
XP_006722663.1. XM_006722600.1. [Q9Y2X0-1 ]
UniGenei Hs.365207.

3D structure databases

ProteinModelPortali Q9Y2X0.
SMRi Q9Y2X0. Positions 71-113.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115342. 46 interactions.
DIPi DIP-31463N.
IntActi Q9Y2X0. 12 interactions.
MINTi MINT-6744281.
STRINGi 9606.ENSP00000325612.

PTM databases

PhosphoSitei Q9Y2X0.

Polymorphism databases

DMDMi 62511180.

Proteomic databases

MaxQBi Q9Y2X0.
PaxDbi Q9Y2X0.
PRIDEi Q9Y2X0.

Protocols and materials databases

DNASUi 10025.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000269814 ; ENSP00000269814 ; ENSG00000175221 . [Q9Y2X0-4 ]
ENST00000312090 ; ENSP00000308528 ; ENSG00000175221 . [Q9Y2X0-3 ]
ENST00000325464 ; ENSP00000325612 ; ENSG00000175221 . [Q9Y2X0-1 ]
ENST00000395808 ; ENSP00000379153 ; ENSG00000175221 . [Q9Y2X0-2 ]
ENST00000589119 ; ENSP00000464810 ; ENSG00000175221 . [Q9Y2X0-1 ]
GeneIDi 10025.
KEGGi hsa:10025.
UCSCi uc002lqd.1. human. [Q9Y2X0-1 ]
uc002lqe.3. human. [Q9Y2X0-2 ]
uc002lqf.3. human. [Q9Y2X0-3 ]
uc010drw.2. human. [Q9Y2X0-4 ]

Organism-specific databases

CTDi 10025.
GeneCardsi GC19M000889.
H-InvDB HIX0015548.
HGNCi HGNC:17556. MED16.
HPAi HPA030685.
HPA046495.
MIMi 604062. gene.
neXtProti NX_Q9Y2X0.
PharmGKBi PA162395406.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG85731.
HOGENOMi HOG000113526.
HOVERGENi HBG054317.
InParanoidi Q9Y2X0.
KOi K15159.
OMAi MRCTTDP.
OrthoDBi EOG7NCV3M.
PhylomeDBi Q9Y2X0.

Enzyme and pathway databases

Reactomei REACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.
SignaLinki Q9Y2X0.

Miscellaneous databases

GeneWikii MED16.
GenomeRNAii 10025.
NextBioi 37883.
PROi Q9Y2X0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Y2X0.
Bgeei Q9Y2X0.
CleanExi HS_MED16.
Genevestigatori Q9Y2X0.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
InterProi IPR021665. Mediator_Med16.
IPR011041. Quinoprot_gluc/sorb_DH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF11635. Med16. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view ]
SMARTi SM00320. WD40. 1 hit.
[Graphical view ]
SUPFAMi SSF50952. SSF50952. 4 hits.
SSF50978. SSF50978. 1 hit.
PROSITEi PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators."
    Ito M., Yuan C.-X., Malik S., Gu W., Fondell J.D., Yamamura S., Fu Z.-Y., Zhang X., Qin J., Roeder R.G.
    Mol. Cell 3:361-370(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 277-283, IDENTIFICATION IN TRAP COMPLEX, FUNCTION OF TRAP COMPLEX.
    Tissue: Cervix carcinoma.
  2. "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex."
    Rachez C., Lemon B.D., Suldan Z., Bromleigh V., Gamble M., Naeaer A.M., Erdjument-Bromage H., Tempst P., Freedman L.P.
    Nature 398:824-828(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), PROTEIN SEQUENCE OF 27-42 AND 755-772, IDENTIFICATION IN ARC COMPLEX, FUNCTION OF ARC COMPLEX.
    Tissue: B-cell.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 248-877 (ISOFORM 4).
    Tissue: Skin and Uterus.
  4. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  5. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED1; MED10; MED21 AND MED30, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

Entry informationi

Entry nameiMED16_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2X0
Secondary accession number(s): Q6PJT2
, Q96AD4, Q96I35, Q9Y652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: September 3, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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