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Q9Y2X0 (MED16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 16
Alternative name(s):
Mediator complex subunit 16
Thyroid hormone receptor-associated protein 5
Thyroid hormone receptor-associated protein complex 95 kDa component
Short name=Trap95
Vitamin D3 receptor-interacting protein complex 92 kDa component
Short name=DRIP92
Gene names
Name:MED16
Synonyms:DRIP92, THRAP5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length877 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.1 Ref.2

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Ref.1 Ref.2 Ref.4 Ref.5

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the Mediator complex subunit 16 family.

Contains 5 WD repeats.

Caution

It is uncertain whether Met-1 or Met-13 is the initiator.

Sequence caution

The sequence AAD31087.1 differs from that shown. Reason: Frameshift at position 609.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
WD repeat
   Molecular functionActivator
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processandrogen receptor signaling pathway

Inferred from direct assay PubMed 12218053. Source: UniProtKB

gene expression

Traceable author statement. Source: Reactome

intracellular steroid hormone receptor signaling pathway

Inferred from direct assay PubMed 11867769. Source: UniProtKB

positive regulation of receptor activity

Inferred from direct assay Ref.1. Source: GOC

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.1. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Non-traceable author statement Ref.1. Source: UniProtKB

transcription from RNA polymerase II promoter

Traceable author statement Ref.1. Source: ProtInc

transcription initiation from RNA polymerase II promoter

Inferred from direct assay PubMed 12218053. Source: UniProtKB

   Cellular_componentmediator complex

Inferred from direct assay Ref.1. Source: UniProtKB

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Non-traceable author statement Ref.1. Source: UniProtKB

   Molecular_functioncatalytic activity

Inferred from electronic annotation. Source: InterPro

receptor activity

Inferred from direct assay PubMed 12218053. Source: UniProtKB

thyroid hormone receptor binding

Inferred from direct assay Ref.1. Source: UniProtKB

thyroid hormone receptor coactivator activity

Inferred from direct assay Ref.1. Source: UniProtKB

transcription coactivator activity

Traceable author statement Ref.1. Source: ProtInc

transcription cofactor activity

Inferred from direct assay PubMed 12218053. Source: UniProtKB

vitamin D receptor binding

Non-traceable author statement Ref.2. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q9Y2X0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y2X0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     829-877: AVEGRGPDACVTSRASEEAPAFVQLGPQSTHHSPRTPRSLDHLHPEDRP → CGGLWWRVPLSYP
Isoform 3 (identifier: Q9Y2X0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     700-700: C → FPSTGPCSVWVLLGWQPLPG
     829-877: AVEGRGPDACVTSRASEEAPAFVQLGPQSTHHSPRTPRSLDHLHPEDRP → CGGLWWRVPLSYP
Note: No experimental confirmation available.
Isoform 4 (identifier: Q9Y2X0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     636-751: GSLLRPGHSF...LQPKQPLRLQ → VAMRAQRASR...CSSRPTEPRR
     752-877: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q9Y2X0-5)

The sequence of this isoform differs from the canonical sequence as follows:
     636-663: GSLLRPGHSFLRDGTSLGMLRELMVVIR → PCPTSEPCPTSEPSPTSEPSPTSEPSSP
     664-877: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 877877Mediator of RNA polymerase II transcription subunit 16
PRO_0000051292

Regions

Repeat79 – 11840WD 1
Repeat210 – 25243WD 2
Repeat275 – 31945WD 3
Repeat634 – 67542WD 4
Repeat790 – 82940WD 5

Natural variations

Alternative sequence636 – 751116GSLLR…PLRLQ → VAMRAQRASRTRRWWMNAAC CPASCLSPAWTGCQPATAWL AACSPSSPFVCSLAGRPRCL AVLPPCSSTASPGPQASPRS TTCGGCTLALAPRRNARPAP GAAVSPCSSRPTEPRR in isoform 4.
VSP_051721
Alternative sequence636 – 66328GSLLR…MVVIR → PCPTSEPCPTSEPSPTSEPS PTSEPSSP in isoform 5.
VSP_028749
Alternative sequence664 – 877214Missing in isoform 5.
VSP_028750
Alternative sequence7001C → FPSTGPCSVWVLLGWQPLPG in isoform 3.
VSP_051722
Alternative sequence752 – 877126Missing in isoform 4.
VSP_051723
Alternative sequence829 – 87749AVEGR…PEDRP → CGGLWWRVPLSYP in isoform 2 and isoform 3. Ref.2
VSP_051724
Natural variant7701L → F.
Corresponds to variant rs34859566 [ dbSNP | Ensembl ].
VAR_053958
Natural variant8741E → K.
Corresponds to variant rs13090 [ dbSNP | Ensembl ].
VAR_053959

Experimental info

Sequence conflict461A → T in AAD30032. Ref.1
Sequence conflict2111G → A in AAD31087. Ref.2
Sequence conflict4121R → K in AAD30032. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 12, 2005. Version 2.
Checksum: 15173F237A75D230

FASTA87796,793
        10         20         30         40         50         60 
MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR 

        70         80         90        100        110        120 
MIHILDTEHP WDLHSIPSEH HEAITCLEWD QSGSRLLSAD ADGQIKCWSM ADHLANSWES 

       130        140        150        160        170        180 
SVGSLVEGDP IVALSWLHNG VKLALHVEKS GASSFGEKFS RVKFSPSLTL FGGKPMEGWI 

       190        200        210        220        230        240 
AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAT ADGSSASPVQ 

       250        260        270        280        290        300 
FYKVCVSVVS EKCRIDTEIL PSLFMRCTTD LNRKDKFPAI THLKFLARDM SEQVLLCASS 

       310        320        330        340        350        360 
QTSSIVECWS LRKEGLPVNN IFQQISPVVG DKQPTILKWR ILSATNDLDR VSAVALPKLP 

       370        380        390        400        410        420 
ISLTNTDLKV ASDTQFYPGL GLALAFHDGS VHIVHRLSLQ TMAVFYSSAA PRPVDEPAMK 

       430        440        450        460        470        480 
RPRTAGPAVH LKAMQLSWTS LALVGIDSHG KLSVLRLSPS MGHPLEVGLA LRHLLFLLEY 

       490        500        510        520        530        540 
CMVTGYDWWD ILLHVQPSMV QSLVEKLHEE YTRQTAALQQ VLSTRILAMK ASLCKLSPCT 

       550        560        570        580        590        600 
VTRVCDYHTK LFLIAISSTL KSLLRPHFLN TPDKSPGDRL TEICTKITDV DIDKVMINLK 

       610        620        630        640        650        660 
TEEFVLDMNT LQALQQLLQW VGDFVLYLLA SLPNQGSLLR PGHSFLRDGT SLGMLRELMV 

       670        680        690        700        710        720 
VIRIWGLLKP SCLPVYTATS DTQDSMSLLF RLLTKLWICC RDEGPASEPD EALVDECCLL 

       730        740        750        760        770        780 
PSQLLIPSLD WLPASDGLVS RLQPKQPLRL QFGRAPTLPG SAATLQLDGL ARAPGQPKID 

       790        800        810        820        830        840 
HLRRLHLGAC PTEECKACTR CGCVTMLKSP NRTTAVKQWE QRWIKNCLAV EGRGPDACVT 

       850        860        870 
SRASEEAPAF VQLGPQSTHH SPRTPRSLDH LHPEDRP 

« Hide

Isoform 2 [UniParc].

Checksum: 2D9CAF68001320C3
Show »

FASTA84193,029
Isoform 3 [UniParc].

Checksum: 546EA25BE3AB56CF
Show »

FASTA86095,050
Isoform 4 [UniParc].

Checksum: 6B77C11B864902BD
Show »

FASTA75182,179
Isoform 5 [UniParc].

Checksum: 311F7B98C3912283
Show »

FASTA66372,967

References

« Hide 'large scale' references
[1]"Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators."
Ito M., Yuan C.-X., Malik S., Gu W., Fondell J.D., Yamamura S., Fu Z.-Y., Zhang X., Qin J., Roeder R.G.
Mol. Cell 3:361-370(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 277-283, IDENTIFICATION IN TRAP COMPLEX, FUNCTION OF TRAP COMPLEX.
Tissue: Cervix carcinoma.
[2]"Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex."
Rachez C., Lemon B.D., Suldan Z., Bromleigh V., Gamble M., Naeaer A.M., Erdjument-Bromage H., Tempst P., Freedman L.P.
Nature 398:824-828(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), PROTEIN SEQUENCE OF 27-42 AND 755-772, IDENTIFICATION IN ARC COMPLEX, FUNCTION OF ARC COMPLEX.
Tissue: B-cell.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 248-877 (ISOFORM 4).
Tissue: Skin and Uterus.
[4]"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[5]"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MED1; MED10; MED21 AND MED30, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF121228 mRNA. Translation: AAD30032.1.
AF106934 mRNA. Translation: AAD31087.1. Frameshift.
BC004554 mRNA. Translation: AAH04554.1.
BC007853 mRNA. Translation: AAH07853.2.
BC011841 mRNA. Translation: AAH11841.1.
BC017282 mRNA. Translation: AAH17282.1.
CCDSCCDS12047.1. [Q9Y2X0-1]
RefSeqNP_005472.2. NM_005481.2. [Q9Y2X0-1]
XP_006722663.1. XM_006722600.1. [Q9Y2X0-1]
UniGeneHs.365207.

3D structure databases

ProteinModelPortalQ9Y2X0.
SMRQ9Y2X0. Positions 71-113.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115342. 46 interactions.
DIPDIP-31463N.
IntActQ9Y2X0. 12 interactions.
MINTMINT-6744281.
STRING9606.ENSP00000325612.

PTM databases

PhosphoSiteQ9Y2X0.

Polymorphism databases

DMDM62511180.

Proteomic databases

MaxQBQ9Y2X0.
PaxDbQ9Y2X0.
PRIDEQ9Y2X0.

Protocols and materials databases

DNASU10025.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000269814; ENSP00000269814; ENSG00000175221. [Q9Y2X0-4]
ENST00000312090; ENSP00000308528; ENSG00000175221. [Q9Y2X0-3]
ENST00000325464; ENSP00000325612; ENSG00000175221. [Q9Y2X0-1]
ENST00000395808; ENSP00000379153; ENSG00000175221. [Q9Y2X0-2]
ENST00000589119; ENSP00000464810; ENSG00000175221. [Q9Y2X0-1]
GeneID10025.
KEGGhsa:10025.
UCSCuc002lqd.1. human. [Q9Y2X0-1]
uc002lqe.3. human. [Q9Y2X0-2]
uc002lqf.3. human. [Q9Y2X0-3]
uc010drw.2. human. [Q9Y2X0-4]

Organism-specific databases

CTD10025.
GeneCardsGC19M000889.
H-InvDBHIX0015548.
HGNCHGNC:17556. MED16.
HPAHPA030685.
HPA046495.
MIM604062. gene.
neXtProtNX_Q9Y2X0.
PharmGKBPA162395406.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG85731.
HOGENOMHOG000113526.
HOVERGENHBG054317.
InParanoidQ9Y2X0.
KOK15159.
OMAMRCTTDP.
OrthoDBEOG7NCV3M.
PhylomeDBQ9Y2X0.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
REACT_71. Gene Expression.
SignaLinkQ9Y2X0.

Gene expression databases

ArrayExpressQ9Y2X0.
BgeeQ9Y2X0.
CleanExHS_MED16.
GenevestigatorQ9Y2X0.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR021665. Mediator_Med16.
IPR011041. Quinoprot_gluc/sorb_DH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF11635. Med16. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 1 hit.
[Graphical view]
SUPFAMSSF50952. SSF50952. 4 hits.
SSF50978. SSF50978. 1 hit.
PROSITEPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMED16.
GenomeRNAi10025.
NextBio37883.
PROQ9Y2X0.
SOURCESearch...

Entry information

Entry nameMED16_HUMAN
AccessionPrimary (citable) accession number: Q9Y2X0
Secondary accession number(s): Q6PJT2 expand/collapse secondary AC list , Q96AD4, Q96I35, Q9Y652
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM