Q9Y2U9 (KLDC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kelch domain-containing protein 2

Alternative name(s):
Hepatocellular carcinoma-associated antigen 33
Host cell factor homolog LCP
Host cell factor-like protein 1
Short name=HCLP-1

Gene names

Name:	KLHDC2
Synonyms:	HCA33

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses CREB3-mediated transcription by interfering with CREB3-DNA binding. Ref.1
Subunit structure	Directly interacts with CREB3, but not with CREB1, ATF4, ATF6, JUN, FOS, CEBPA and herpes simplex virus transactivator VP16. Ref.1
Subcellular location	Nucleus Ref.1.
Tissue specificity	Widely expressed, with high levels in skeletal muscle, heart, pancreas and liver. Undetectable in peripheral blood leukocytes. Ref.1 Ref.8
Sequence similarities	Contains 6 Kelch repeats.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Kelch repeat Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2U9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2U9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     239-249: DARMNDLHYLN → VSIQTTSMTCF
     250-406: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Kelch domain-containing protein 2		PRO_0000119127
Regions
	Repeat	43 – 89	47	Kelch 1
	Repeat	102 – 155	54	Kelch 2
	Repeat	157 – 202	46	Kelch 3
	Repeat	229 – 278	50	Kelch 4
	Repeat	280 – 329	50	Kelch 5
	Repeat	331 – 377	47	Kelch 6
Natural variations
	Alternative sequence	239 – 249	11	DARMNDLHYLN → VSIQTTSMTCF in isoform 2.		VSP_030165
	Alternative sequence	250 – 406	157	Missing in isoform 2.		VSP_030166
Experimental info
	Sequence conflict	25	1	E → D in CAG33486. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 07486B06AC4E97FF	FASTA	406	46,099
	10 20 30 40 50 60 MADGNEDLRA DDLPGPAFES YESMELACPA ERSGHVAVSD GRHMFVWGGY KSNQVRGLYD 70 80 90 100 110 120 FYLPREELWI YNMETGRWKK INTEGDVPPS MSGSCAVCVD RVLYLFGGHH SRGNTNKFYM 130 140 150 160 170 180 LDSRSTDRVL QWERIDCQGI PPSSKDKLGV WVYKNKLIFF GGYGYLPEDK VLGTFEFDET 190 200 210 220 230 240 SFWNSSHPRG WNDHVHILDT ETFTWSQPIT TGKAPSPRAA HACATVGNRG FVFGGRYRDA 250 260 270 280 290 300 RMNDLHYLNL DTWEWNELIP QGICPVGRSW HSLTPVSSDH LFLFGGFTTD KQPLSDAWTY 310 320 330 340 350 360 CISKNEWIQF NHPYTEKPRL WHTACASDEG EVIVFGGCAN NLLVHHRAAH SNEILIFSVQ 370 380 390 400 PKSLVRLSLE AVICFKEMLA NSWNCLPKHL LHSVNQRFGS NNTSGS
	Isoform 2 [UniParc]. Checksum: 19ED55962024271A	FASTA	249	28,198
	10 20 30 40 50 60 MADGNEDLRA DDLPGPAFES YESMELACPA ERSGHVAVSD GRHMFVWGGY KSNQVRGLYD 70 80 90 100 110 120 FYLPREELWI YNMETGRWKK INTEGDVPPS MSGSCAVCVD RVLYLFGGHH SRGNTNKFYM 130 140 150 160 170 180 LDSRSTDRVL QWERIDCQGI PPSSKDKLGV WVYKNKLIFF GGYGYLPEDK VLGTFEFDET 190 200 210 220 230 240 SFWNSSHPRG WNDHVHILDT ETFTWSQPIT TGKAPSPRAA HACATVGNRG FVFGGRYRVS IQTTSMTCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inhibition of LZIP-mediated transcription through direct interaction with a novel host cell factor-like protein." Zhou H.-J., Wong C.-M., Chen J.-H., Qiang B.-Q., Yuan J.-G., Jin D.-Y. J. Biol. Chem. 276:28933-28938(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH CREB3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies." Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F. J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hepatoma.
[3]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovarian carcinoma.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Kidney.
[8]	"Differential subcellular localization and activity of kelch repeat proteins KLHDC1 and KLHDC2." Chin K.-T., Xu H.-T., Ching Y.-P., Jin D.-Y. Mol. Cell. Biochem. 296:109-119(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF113131 mRNA. Translation: AAD21038.1. AF244137 mRNA. Translation: AAF66246.1. BX161429 mRNA. Translation: CAD61901.1. AK001771 mRNA. Translation: BAA91898.1. AK056298 mRNA. Translation: BAG51671.1. CR457205 mRNA. Translation: CAG33486.1. CH471078 Genomic DNA. Translation: EAW65749.1. CH471078 Genomic DNA. Translation: EAW65750.1. BC002335 mRNA. Translation: AAH02335.1. BC024192 mRNA. Translation: AAH24192.1.
IPI	IPI00003793. IPI00878805.
RefSeq	NP_055130.1. NM_014315.2.
UniGene	Hs.509264.
3D structure databases
ProteinModelPortal	Q9Y2U9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y2U9. 8 interactions.
MINT	MINT-1371568.
STRING	9606.ENSP00000298307.
PTM databases
PhosphoSite	Q9Y2U9.
Polymorphism databases
DMDM	28380093.
Proteomic databases
PaxDb	Q9Y2U9.
PRIDE	Q9Y2U9.
Protocols and materials databases
DNASU	23588.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000298307; ENSP00000298307; ENSG00000165516. ENST00000555443; ENSP00000450944; ENSG00000165516. ENST00000555739; ENSP00000450539; ENSG00000165516.
GeneID	23588.
KEGG	hsa:23588.
UCSC	uc001wwx.3. human.
Organism-specific databases
CTD	23588.
GeneCards	GC14P050234.
HGNC	HGNC:20231. KLHDC2.
HPA	HPA000628.
MIM	611280. gene.
neXtProt	NX_Q9Y2U9.
PharmGKB	PA134924955.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG321152.
HOGENOM	HOG000006587.
HOVERGEN	HBG067471.
InParanoid	Q9Y2U9.
OMA	LDTETFI.
OrthoDB	EOG45756Q.
PhylomeDB	Q9Y2U9.
Gene expression databases
ArrayExpress	Q9Y2U9.
Bgee	Q9Y2U9.
CleanEx	HS_KLHDC2.
Genevestigator	Q9Y2U9.
GermOnline	ENSG00000165516. Homo sapiens.
Family and domain databases
Gene3D	2.120.10.80. 2 hits.
InterPro	IPR015915. Kelch-typ_b-propeller. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	23588.
NextBio	46222.
SOURCE	Search...

Entry information

Entry name

KLDC2_HUMAN

Accession

Primary (citable) accession number: Q9Y2U9
Secondary accession number(s): B3KPF9, Q6IAF0, Q86TY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.