Q9Y2T7 (YBOX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Y-box-binding protein 2

Alternative name(s):
Contrin
DNA-binding protein C
Short name=Dbpc
Germ cell-specific Y-box-binding protein
MSY2 homolog

Gene names

Name:	YBX2
Synonyms:	CSDA3, MSY2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay By similarity.
Subunit structure	Found in a mRNP complex with PABPC1 and YBX3 By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.4.
Tissue specificity	Expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Also observed placental trophoblasts, as well as in vascular smooth muscle cells in the pulmonary artery, myocardium, and skeletal muscle. Undetectable in epithelial cells in respiratory, gastrointestinal, and urogenital tracts. Up-regulated in various carcinomas and germ cell tumors (at protein level). Ref.1 Ref.4
Post-translational modification	Phosphorylated during oocyte maturation and dephosphorylated following egg activation. Phosphorylated in vitro by a kinase activity associated with testicular mRNPs. Dephosphorylation leads to a decrease in its affinity to bind RNA in vitro By similarity.
Sequence similarities	Contains 1 CSD (cold-shock) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA stabilization Inferred from electronic annotation. Source: Compara negative regulation of binding Inferred from electronic annotation. Source: Compara negative regulation of translation Inferred from electronic annotation. Source: Compara oocyte development Inferred from electronic annotation. Source: Compara regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro spermatid development Inferred from electronic annotation. Source: Compara spermatogenesis Traceable author statement Ref.1. Source: ProtInc transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc translational attenuation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Traceable author statement Ref.1. Source: ProtInc nucleus Traceable author statement Ref.1. Source: ProtInc polysome Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: Compara lipid binding Inferred from electronic annotation. Source: Compara mRNA 3'-UTR binding Inferred from electronic annotation. Source: Compara ribonucleoprotein complex binding Inferred from electronic annotation. Source: Compara translation regulator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Y-box-binding protein 2		PRO_0000100225
Regions
	Domain	93 – 163	71	CSD
	Region	87 – 169	83	Required for cytoplasmic retention By similarity
	Region	217 – 364	148	Required for mRNA-binding
	Compositional bias	35 – 67	33	Gly-rich
	Compositional bias	185 – 352	168	Pro-rich
Natural variations
	Natural variant	9	1	G → V. Ref.1 Ref.2 Ref.3 Corresponds to variant rs222859 [ dbSNP | Ensembl ].		VAR_027916
	Natural variant	63	1	S → P. Corresponds to variant rs8069533 [ dbSNP | Ensembl ].		VAR_027917

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2T7 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 0B16DEF46F4634FA	FASTA	364	38,518
	10 20 30 40 50 60 MSEVEAAAGA TAVPAATVPA TAAGVVAVVV PVPAGEPQKG GGAGGGGGAA SGPAAGTPSA 70 80 90 100 110 120 PGSRTPGNPA TAVSGTPAPP ARSQADKPVL AIQVLGTVKW FNVRNGYGFI NRNDTKEDVF 130 140 150 160 170 180 VHQTAIKRNN PRKFLRSVGD GETVEFDVVE GEKGAEATNV TGPGGVPVKG SRYAPNRRKS 190 200 210 220 230 240 RRFIPRPPSV APPPMVAEIP SAGTGPGSKG ERAEDSGQRP RRWCPPPFFY RRRFVRGPRP 250 260 270 280 290 300 PNQQQPIEGT DRVEPKETAP LEGHQQQGDE RVPPPRFRPR YRRPFRPRPR QQPTTEGGDG 310 320 330 340 350 360 ETKPSQGPAD GSRPEPQRPR NRPYFQRRRQ QAPGPQQAPG PRQPAAPETS APVNSGDPTT TILE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Contrin, the human homologue of a germ-cell Y-box-binding protein: cloning, expression, and chromosomal localization." Tekur S., Pawlak A., Guellaen G., Hecht N.B. J. Androl. 20:135-144(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT VAL-9.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-9.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-9. Tissue: Brain.
[4]	"Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen." Kohno Y., Matsuki Y., Tanimoto A., Izumi H., Uchiumi T., Kohno K., Shimajiri S., Sasaguri Y. Br. J. Cancer 94:710-716(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF096834 mRNA. Translation: AAD30662.1. CH471108 Genomic DNA. Translation: EAW90223.1. CH471108 Genomic DNA. Translation: EAW90224.1. BC033800 mRNA. Translation: AAH33800.1. BC047760 mRNA. Translation: AAH47760.1.
IPI	IPI00250153.
RefSeq	NP_057066.2. NM_015982.3.
UniGene	Hs.567494.
3D structure databases
ProteinModelPortal	Q9Y2T7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000007699.
PTM databases
PhosphoSite	Q9Y2T7.
Polymorphism databases
DMDM	116242847.
Proteomic databases
PaxDb	Q9Y2T7.
PRIDE	Q9Y2T7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000007699; ENSP00000007699; ENSG00000006047.
GeneID	51087.
KEGG	hsa:51087.
UCSC	uc002gfq.2. human.
Organism-specific databases
CTD	51087.
GeneCards	GC17M007191.
HGNC	HGNC:17948. YBX2.
HPA	CAB012332.
MIM	611447. gene.
neXtProt	NX_Q9Y2T7.
PharmGKB	PA142670560.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1278.
HOGENOM	HOG000116439.
HOVERGEN	HBG079361.
InParanoid	Q9Y2T7.
KO	K09277.
OMA	MEGTDGV.
OrthoDB	EOG46DM3W.
PhylomeDB	Q9Y2T7.
Gene expression databases
Bgee	Q9Y2T7.
CleanEx	HS_YBX2.
Genevestigator	Q9Y2T7.
GermOnline	ENSG00000006047. Homo sapiens.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
InterPro	IPR019844. Cold-shock_CS. IPR011129. Cold_shock_prot. IPR002059. CSP_DNA-bd. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF00313. CSD. 1 hit. [Graphical view]
PRINTS	PR00050. COLDSHOCK.
SMART	SM00357. CSP. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS00352. COLD_SHOCK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	YBX2. human.
GenomeRNAi	51087.
NextBio	53755.
SOURCE	Search...

Entry information

Entry name

YBOX2_HUMAN

Accession

Primary (citable) accession number: Q9Y2T7
Secondary accession number(s): D3DTP1, Q8N4P0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 17, 2006
Last modified:	May 29, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.