Reviewed, UniProtKB/Swiss-Prot Q9Y2T7 (YBOX2_HUMAN)

Names and origin

Protein names

Recommended name:
    Y-box-binding protein 2
Alternative name(s):
    Germ cell-specific Y-box-binding protein
    Contrin
    MSY2 homolog
    DNA-binding protein C
      Short name=Dbpc

Gene names

Name:	YBX2
Synonyms:	CSDA3, MSY2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay By similarity.
Subunit structure	Found in a mRNP complex with PABPC1 and CSDA By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.3.
Tissue specificity	Expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Also observed placental trophoblasts, as well as in vascular smooth muscle cells in the pulmonary artery, myocardium, and skeletal muscle. Undetectable in epithelial cells in respiratory, gastrointestinal, and urogenital tracts. Up-regulated in various carcinomas and germ cell tumors (at protein level). Ref.3 Ref.1
Post-translational modification	Phosphorylated during oocyte maturation and dephosphorylated following egg activation. Phosphorylated in vitro by a kinase activity associated with testicular mRNPs. Dephosphorylation leads to a decrease in its affinity to bind RNA in vitro By similarity.
Sequence similarities	Contains 1 CSD (cold-shock) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc translational attenuation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Y-box-binding protein 2		PRO_0000100225
Regions
	Domain	93 – 163	71	CSD
	Region	87 – 169	83	Required for cytoplasmic retention By similarity
	Region	217 – 364	148	Required for mRNA-binding
	Compositional bias	35 – 67	33	Gly-rich
	Compositional bias	185 – 352	168	Pro-rich
Amino acid modifications
	Modified residue	116	1	N6-acetyllysine Ref.4
Natural variations
	Natural variant	9	1	G → V: dbSNP rs222859. Ref.1 Ref.2		VAR_027916
	Natural variant	63	1	S → P: dbSNP rs8069533.		VAR_027917

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2T7-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 0B16DEF46F4634FA	FASTA	364	38,518
	10 20 30 40 50 60 MSEVEAAAGA TAVPAATVPA TAAGVVAVVV PVPAGEPQKG GGAGGGGGAA SGPAAGTPSA 70 80 90 100 110 120 PGSRTPGNPA TAVSGTPAPP ARSQADKPVL AIQVLGTVKW FNVRNGYGFI NRNDTKEDVF 130 140 150 160 170 180 VHQTAIKRNN PRKFLRSVGD GETVEFDVVE GEKGAEATNV TGPGGVPVKG SRYAPNRRKS 190 200 210 220 230 240 RRFIPRPPSV APPPMVAEIP SAGTGPGSKG ERAEDSGQRP RRWCPPPFFY RRRFVRGPRP 250 260 270 280 290 300 PNQQQPIEGT DRVEPKETAP LEGHQQQGDE RVPPPRFRPR YRRPFRPRPR QQPTTEGGDG 310 320 330 340 350 360 ETKPSQGPAD GSRPEPQRPR NRPYFQRRRQ QAPGPQQAPG PRQPAAPETS APVNSGDPTT TILE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Contrin, the human homologue of a germ-cell Y-box-binding protein: cloning, expression, and chromosomal localization." Tekur S., Pawlak A., Guellaen G., Hecht N.B. J. Androl. 20:135-144(1999) [PubMed: 10100484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT VAL-9.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-9. Tissue: Brain.
[3]	"Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen." Kohno Y., Matsuki Y., Tanimoto A., Izumi H., Uchiumi T., Kohno K., Shimajiri S., Sasaguri Y. Br. J. Cancer 94:710-716(2006) [PubMed: 16479255] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[4]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-116, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF096834 mRNA. Translation: AAD30662.1. BC033800 mRNA. Translation: AAH33800.1. BC047760 mRNA. Translation: AAH47760.1.
IPI	IPI00250153.
RefSeq	NP_057066.2.
UniGene	Hs.567494
3D structure databases
SMR	Q9Y2T7. Positions 87-164.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y2T7.
PTM databases
PhosphoSite	Q9Y2T7.
Proteomic databases
PRIDE	Q9Y2T7.
Genome annotation databases
Ensembl	ENST00000007699; ENSP00000007699; ENSG00000006047; Homo sapiens. [Genome view]
GeneID	51087.
KEGG	hsa:51087.
UCSC	uc002gfq.2. human.
Organism-specific databases
CTD	51087.
GeneCards	GC17M007132.
H-InvDB	HIX0013491.
HGNC	HGNC:17948. YBX2.
HPA	CAB012332.
MIM	611447. gene.
PharmGKB	PA142670560.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG125145.
HOVERGEN	Q9Y2T7.
InParanoid	Q9Y2T7.
OMA	NQQQPIE.
OrthoDB	EOG98PQ4Q.
PhylomeDB	Q9Y2T7.
Gene expression databases
ArrayExpress	Q9Y2T7.
Bgee	Q9Y2T7.
CleanEx	HS_YBX2.
Genevestigator	Q9Y2T7.
GermOnline	ENSG00000006047. Homo sapiens.
Family and domain databases
InterPro	IPR019844. Cold-shock_CS. IPR011129. Cold_shock_prot. IPR002059. CSP_DNA_bd. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00313. CSD. 1 hit. [Graphical view]
PRINTS	PR00050. COLDSHOCK.
SMART	SM00357. CSP. 1 hit. [Graphical view]
PROSITE	PS00352. COLD_SHOCK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	53755.
SOURCE	Search...

Entry information

Entry name

YBOX2_HUMAN

Accession

Primary (citable) accession number: Q9Y2T7
Secondary accession number(s): Q8N4P0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 17, 2006
Last modified:	January 19, 2010
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.