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Protein

G-protein coupled receptor 55

Gene

GPR55

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca2+ release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.By similarity1 Publication

GO - Molecular functioni

  • cannabinoid receptor activity Source: UniProtKB
  • G-protein coupled receptor activity Source: ProtInc

GO - Biological processi

  • activation of phospholipase C activity Source: UniProtKB
  • bone resorption Source: UniProtKB
  • cannabinoid signaling pathway Source: GOC
  • G-protein coupled receptor signaling pathway Source: ProtInc
  • negative regulation of osteoclast differentiation Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of Rho protein signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_14828. Class A/1 (Rhodopsin-like receptors).
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 55
Gene namesi
Name:GPR55
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:4511. GPR55.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121ExtracellularSequence AnalysisAdd
BLAST
Transmembranei22 – 4221Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini43 – 5816CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei59 – 7921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini80 – 9415ExtracellularSequence AnalysisAdd
BLAST
Transmembranei95 – 11521Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini116 – 13722CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei138 – 15821Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini159 – 18022ExtracellularSequence AnalysisAdd
BLAST
Transmembranei181 – 20121Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini202 – 23130CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei232 – 25221Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini253 – 27119ExtracellularSequence AnalysisAdd
BLAST
Transmembranei272 – 29221Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini293 – 31927CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28900.

Polymorphism and mutation databases

BioMutaiGPR55.
DMDMi71159390.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319G-protein coupled receptor 55PRO_0000069577Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9Y2T6.
PRIDEiQ9Y2T6.

PTM databases

PhosphoSiteiQ9Y2T6.

Expressioni

Tissue specificityi

Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.2 Publications

Gene expression databases

BgeeiQ9Y2T6.
CleanExiHS_GPR55.
ExpressionAtlasiQ9Y2T6. baseline and differential.
GenevisibleiQ9Y2T6. HS.

Interactioni

Protein-protein interaction databases

BioGridi114705. 3 interactions.
STRINGi9606.ENSP00000375893.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2T6.
SMRiQ9Y2T6. Positions 25-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145477.
GeneTreeiENSGT00730000110768.
HOGENOMiHOG000013155.
HOVERGENiHBG096388.
InParanoidiQ9Y2T6.
KOiK08413.
OMAiCFHNMSD.
OrthoDBiEOG7327PC.
PhylomeDBiQ9Y2T6.
TreeFamiTF335700.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR028334. GPR55.
[Graphical view]
PANTHERiPTHR24232:SF8. PTHR24232:SF8. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y2T6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQNTSGDC LFDGVNELMK TLQFAVHIPT FVLGLLLNLL AIHGFSTFLK
60 70 80 90 100
NRWPDYAATS IYMINLAVFD LLLVLSLPFK MVLSQVQSPF PSLCTLVECL
110 120 130 140 150
YFVSMYGSVF TICFISMDRF LAIRYPLLVS HLRSPRKIFG ICCTIWVLVW
160 170 180 190 200
TGSIPIYSFH GKVEKYMCFH NMSDDTWSAK VFFPLEVFGF LLPMGIMGFC
210 220 230 240 250
CSRSIHILLG RRDHTQDWVQ QKACIYSIAA SLAVFVVSFL PVHLGFFLQF
260 270 280 290 300
LVRNSFIVEC RAKQSISFFL QLSMCFSNVN CCLDVFCYYF VIKEFRMNIR
310
AHRPSRVQLV LQDTTISRG
Length:319
Mass (Da):36,637
Last modified:May 10, 2005 - v2
Checksum:iD6E5C6CA8426E7D5
GO

Sequence cautioni

The sequence AAD22410.1 differs from that shown. Reason: Frameshift at positions 132 and 142. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti195 – 1951G → V.
Corresponds to variant rs3749073 [ dbSNP | Ensembl ].
VAR_024257
Natural varianti215 – 2151T → N.
Corresponds to variant rs34229723 [ dbSNP | Ensembl ].
VAR_049395

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096786 Genomic DNA. Translation: AAD22410.1. Frameshift.
CR541776 mRNA. Translation: CAG46575.1.
BC032694 mRNA. Translation: AAH32694.1.
CCDSiCCDS2480.1.
RefSeqiNP_005674.2. NM_005683.3.
XP_005247009.1. XM_005246952.2.
XP_011510477.1. XM_011512175.1.
XP_011510478.1. XM_011512176.1.
XP_011510479.1. XM_011512177.1.
UniGeneiHs.114545.

Genome annotation databases

EnsembliENST00000392039; ENSP00000375893; ENSG00000135898.
ENST00000392040; ENSP00000375894; ENSG00000135898.
ENST00000444078; ENSP00000410267; ENSG00000135898.
ENST00000622008; ENSP00000482381; ENSG00000135898.
GeneIDi9290.
KEGGihsa:9290.
UCSCiuc002vrg.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096786 Genomic DNA. Translation: AAD22410.1. Frameshift.
CR541776 mRNA. Translation: CAG46575.1.
BC032694 mRNA. Translation: AAH32694.1.
CCDSiCCDS2480.1.
RefSeqiNP_005674.2. NM_005683.3.
XP_005247009.1. XM_005246952.2.
XP_011510477.1. XM_011512175.1.
XP_011510478.1. XM_011512176.1.
XP_011510479.1. XM_011512177.1.
UniGeneiHs.114545.

3D structure databases

ProteinModelPortaliQ9Y2T6.
SMRiQ9Y2T6. Positions 25-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114705. 3 interactions.
STRINGi9606.ENSP00000375893.

Chemistry

BindingDBiQ9Y2T6.
ChEMBLiCHEMBL1075322.
GuidetoPHARMACOLOGYi109.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9Y2T6.

Polymorphism and mutation databases

BioMutaiGPR55.
DMDMi71159390.

Proteomic databases

PaxDbiQ9Y2T6.
PRIDEiQ9Y2T6.

Protocols and materials databases

DNASUi9290.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000392039; ENSP00000375893; ENSG00000135898.
ENST00000392040; ENSP00000375894; ENSG00000135898.
ENST00000444078; ENSP00000410267; ENSG00000135898.
ENST00000622008; ENSP00000482381; ENSG00000135898.
GeneIDi9290.
KEGGihsa:9290.
UCSCiuc002vrg.3. human.

Organism-specific databases

CTDi9290.
GeneCardsiGC02M231736.
HGNCiHGNC:4511. GPR55.
MIMi604107. gene.
neXtProtiNX_Q9Y2T6.
PharmGKBiPA28900.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG145477.
GeneTreeiENSGT00730000110768.
HOGENOMiHOG000013155.
HOVERGENiHBG096388.
InParanoidiQ9Y2T6.
KOiK08413.
OMAiCFHNMSD.
OrthoDBiEOG7327PC.
PhylomeDBiQ9Y2T6.
TreeFamiTF335700.

Enzyme and pathway databases

ReactomeiREACT_14828. Class A/1 (Rhodopsin-like receptors).
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

GeneWikiiGPR55.
GenomeRNAii9290.
NextBioi34813.
PROiQ9Y2T6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y2T6.
CleanExiHS_GPR55.
ExpressionAtlasiQ9Y2T6. baseline and differential.
GenevisibleiQ9Y2T6. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR028334. GPR55.
[Graphical view]
PANTHERiPTHR24232:SF8. PTHR24232:SF8. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and cloning of three novel human G protein-coupled receptor genes GPR52, PsiGPR53 and GPR55: GPR55 is extensively expressed in human brain."
    Sawzdargo M., Nguyen T., Lee D.K., Lynch K.R., Cheng R., Heng H.H.Q., George S.R., O'Dowd B.F.
    Brain Res. Mol. Brain Res. 64:193-198(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  4. "Identification of GPR55 as a lysophosphatidylinositol receptor."
    Oka S., Nakajima K., Yamashita A., Kishimoto S., Sugiura T.
    Biochem. Biophys. Res. Commun. 362:928-934(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: LIGAND-BINDING.
  5. "The GPR55 ligand L-alpha-lysophosphatidylinositol promotes RhoA-dependent Ca2+ signaling and NFAT activation."
    Henstridge C.M., Balenga N.A., Ford L.A., Ross R.A., Waldhoer M., Irving A.J.
    FASEB J. 23:183-193(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, CHARACTERIZATION, LIGAND-BINDING.
  6. "Atypical responsiveness of the orphan receptor GPR55 to cannabinoid ligands."
    Kapur A., Zhao P., Sharir H., Bai Y., Caron M.G., Barak L.S., Abood M.E.
    J. Biol. Chem. 284:29817-29827(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: LIGAND-BINDING.
  7. "The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo."
    Whyte L.S., Ryberg E., Sims N.A., Ridge S.A., Mackie K., Greasley P.J., Ross R.A., Rogers M.J.
    Proc. Natl. Acad. Sci. U.S.A. 106:16511-16516(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION.

Entry informationi

Entry nameiGPR55_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2T6
Secondary accession number(s): Q8N580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: July 22, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The classification of this protein as a cannabinoid receptor remains a contentious issue due to conflicting pharmacological results.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.