Q9Y2T6 (GPR55_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor 55

Gene names

Name:

GPR55

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in hyperalgesia associated with inflammatory and neuropathic pain By similarity. Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca2+ release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function. Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.5.
Tissue specificity	Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts. Ref.1 Ref.7
Miscellaneous	The classification of this protein as a cannabinoid receptor remains a contentious issue due to conflicting pharmacological results.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAD22410.1 differs from that shown. Reason: Frameshift at positions 132 and 142.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of phospholipase C activity Inferred from direct assay Ref.5. Source: UniProtKB bone resorption Inferred from direct assay Ref.7. Source: UniProtKB negative regulation of osteoclast differentiation Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of ERK1 and ERK2 cascade Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of Rho protein signal transduction Inferred from direct assay Ref.5Ref.7. Source: UniProtKB
Cellular_component	integral to plasma membrane Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	cannabinoid receptor activity Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	G-protein coupled receptor 55		PRO_0000069577
Regions
	Topological domain	1 – 21	21	Extracellular Potential
	Transmembrane	22 – 42	21	Helical; Name=1; Potential
	Topological domain	43 – 58	16	Cytoplasmic Potential
	Transmembrane	59 – 79	21	Helical; Name=2; Potential
	Topological domain	80 – 94	15	Extracellular Potential
	Transmembrane	95 – 115	21	Helical; Name=3; Potential
	Topological domain	116 – 137	22	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Helical; Name=4; Potential
	Topological domain	159 – 180	22	Extracellular Potential
	Transmembrane	181 – 201	21	Helical; Name=5; Potential
	Topological domain	202 – 231	30	Cytoplasmic Potential
	Transmembrane	232 – 252	21	Helical; Name=6; Potential
	Topological domain	253 – 271	19	Extracellular Potential
	Transmembrane	272 – 292	21	Helical; Name=7; Potential
	Topological domain	293 – 319	27	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	195	1	G → V. Corresponds to variant rs3749073 [ dbSNP | Ensembl ].		VAR_024257
	Natural variant	215	1	T → N. Corresponds to variant rs34229723 [ dbSNP | Ensembl ].		VAR_049395

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2T6 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: D6E5C6CA8426E7D5	FASTA	319	36,637
	10 20 30 40 50 60 MSQQNTSGDC LFDGVNELMK TLQFAVHIPT FVLGLLLNLL AIHGFSTFLK NRWPDYAATS 70 80 90 100 110 120 IYMINLAVFD LLLVLSLPFK MVLSQVQSPF PSLCTLVECL YFVSMYGSVF TICFISMDRF 130 140 150 160 170 180 LAIRYPLLVS HLRSPRKIFG ICCTIWVLVW TGSIPIYSFH GKVEKYMCFH NMSDDTWSAK 190 200 210 220 230 240 VFFPLEVFGF LLPMGIMGFC CSRSIHILLG RRDHTQDWVQ QKACIYSIAA SLAVFVVSFL 250 260 270 280 290 300 PVHLGFFLQF LVRNSFIVEC RAKQSISFFL QLSMCFSNVN CCLDVFCYYF VIKEFRMNIR 310 AHRPSRVQLV LQDTTISRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and cloning of three novel human G protein-coupled receptor genes GPR52, PsiGPR53 and GPR55: GPR55 is extensively expressed in human brain." Sawzdargo M., Nguyen T., Lee D.K., Lynch K.R., Cheng R., Heng H.H.Q., George S.R., O'Dowd B.F. Brain Res. Mol. Brain Res. 64:193-198(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[4]	"Identification of GPR55 as a lysophosphatidylinositol receptor." Oka S., Nakajima K., Yamashita A., Kishimoto S., Sugiura T. Biochem. Biophys. Res. Commun. 362:928-934(2007) [PubMed] [Europe PMC] [Abstract] Cited for: LIGAND-BINDING.
[5]	"The GPR55 ligand L-alpha-lysophosphatidylinositol promotes RhoA-dependent Ca2+ signaling and NFAT activation." Henstridge C.M., Balenga N.A., Ford L.A., Ross R.A., Waldhoer M., Irving A.J. FASEB J. 23:183-193(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, CHARACTERIZATION, LIGAND-BINDING.
[6]	"Atypical responsiveness of the orphan receptor GPR55 to cannabinoid ligands." Kapur A., Zhao P., Sharir H., Bai Y., Caron M.G., Barak L.S., Abood M.E. J. Biol. Chem. 284:29817-29827(2009) [PubMed] [Europe PMC] [Abstract] Cited for: LIGAND-BINDING.
[7]	"The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo." Whyte L.S., Ryberg E., Sims N.A., Ridge S.A., Mackie K., Greasley P.J., Ross R.A., Rogers M.J. Proc. Natl. Acad. Sci. U.S.A. 106:16511-16516(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF096786 Genomic DNA. Translation: AAD22410.1. Frameshift. CR541776 mRNA. Translation: CAG46575.1. BC032694 mRNA. Translation: AAH32694.1.
IPI	IPI00328871.
RefSeq	NP_005674.2. NM_005683.3.
UniGene	Hs.114545.
3D structure databases
ProteinModelPortal	Q9Y2T6.
SMR	Q9Y2T6. Positions 25-300.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000375893.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9Y2T6.
Polymorphism databases
DMDM	71159390.
Proteomic databases
PaxDb	Q9Y2T6.
PRIDE	Q9Y2T6.
Protocols and materials databases
DNASU	9290.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000392039; ENSP00000375893; ENSG00000135898. ENST00000392040; ENSP00000375894; ENSG00000135898. ENST00000444078; ENSP00000410267; ENSG00000135898.
GeneID	9290.
KEGG	hsa:9290.
UCSC	uc002vrg.3. human.
Organism-specific databases
CTD	9290.
GeneCards	GC02M231736.
HGNC	HGNC:4511. GPR55.
MIM	604107. gene.
neXtProt	NX_Q9Y2T6.
PharmGKB	PA28900.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG145477.
HOGENOM	HOG000013155.
HOVERGEN	HBG096388.
InParanoid	Q9Y2T6.
KO	K08413.
OMA	CFHNMSD.
OrthoDB	EOG4JT063.
PhylomeDB	Q9Y2T6.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9Y2T6.
Bgee	Q9Y2T6.
CleanEx	HS_GPR55.
Genevestigator	Q9Y2T6.
GermOnline	ENSG00000135898. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9Y2T6.
ChEMBL	CHEMBL1075322.
GenomeRNAi	9290.
NextBio	34813.
SOURCE	Search...

Entry information

Entry name

GPR55_HUMAN

Accession

Primary (citable) accession number: Q9Y2T6
Secondary accession number(s): Q8N580

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.