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Protein

Guanine deaminase

Gene

GDA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.By similarity

Catalytic activityi

Guanine + H2O = xanthine + NH3.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: guanine degradation

This protein is involved in step 1 of the subpathway that synthesizes xanthine from guanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanine deaminase (GDA)
This subpathway is part of the pathway guanine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes xanthine from guanine, the pathway guanine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Zinc; via tele nitrogen1
Metal bindingi84Zinc; via tele nitrogen1
Binding sitei87Substrate1
Binding sitei213Substrate1
Metal bindingi240Zinc; via tele nitrogen1
Binding sitei243Substrate1
Binding sitei279Substrate1
Metal bindingi330ZincBy similarity1

GO - Molecular functioni

  • guanine deaminase activity Source: Reactome
  • zinc ion binding Source: ProtInc

GO - Biological processi

  • guanine catabolic process Source: UniProtKB-UniPathway
  • guanine metabolic process Source: GO_Central
  • nervous system development Source: ProtInc
  • nucleobase-containing compound metabolic process Source: ProtInc
  • purine nucleotide catabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS04276-MONOMER.
ZFISH:HS04276-MONOMER.
BRENDAi3.5.4.3. 2681.
ReactomeiR-HSA-74259. Purine catabolism.
SABIO-RKQ9Y2T3.
UniPathwayiUPA00603; UER00660.

Protein family/group databases

MEROPSiM38.981.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine deaminase (EC:3.5.4.3)
Short name:
Guanase
Short name:
Guanine aminase
Alternative name(s):
Guanine aminohydrolase
Short name:
GAH
p51-nedasin
Gene namesi
Name:GDA
Synonyms:KIAA1258
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:4212. GDA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • intracellular Source: ProtInc
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi9615.
OpenTargetsiENSG00000119125.
PharmGKBiPA28625.

Chemistry databases

ChEMBLiCHEMBL3129.

Polymorphism and mutation databases

BioMutaiGDA.
DMDMi9910736.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001222981 – 454Guanine deaminaseAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei453PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y2T3.
PaxDbiQ9Y2T3.
PeptideAtlasiQ9Y2T3.
PRIDEiQ9Y2T3.

PTM databases

iPTMnetiQ9Y2T3.
PhosphoSitePlusiQ9Y2T3.

Expressioni

Gene expression databases

BgeeiENSG00000119125.
CleanExiHS_GDA.
ExpressionAtlasiQ9Y2T3. baseline and differential.
GenevisibleiQ9Y2T3. HS.

Organism-specific databases

HPAiHPA019352.
HPA024099.
HPA030387.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi114976. 36 interactors.
MINTiMINT-109340.
STRINGi9606.ENSP00000238018.

Chemistry databases

BindingDBiQ9Y2T3.

Structurei

Secondary structure

1454
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 19Combined sources9
Beta strandi27 – 36Combined sources10
Beta strandi40 – 47Combined sources8
Helixi48 – 50Combined sources3
Helixi51 – 57Combined sources7
Helixi62 – 64Combined sources3
Beta strandi65 – 67Combined sources3
Beta strandi73 – 76Combined sources4
Beta strandi78 – 84Combined sources7
Helixi85 – 90Combined sources6
Helixi99 – 105Combined sources7
Helixi107 – 112Combined sources6
Helixi113 – 115Combined sources3
Helixi117 – 133Combined sources17
Beta strandi136 – 142Combined sources7
Helixi147 – 160Combined sources14
Beta strandi163 – 167Combined sources5
Beta strandi175 – 177Combined sources3
Helixi184 – 201Combined sources18
Beta strandi204 – 208Combined sources5
Beta strandi210 – 212Combined sources3
Turni215 – 217Combined sources3
Helixi220 – 232Combined sources13
Beta strandi237 – 242Combined sources6
Helixi245 – 254Combined sources10
Beta strandi258 – 260Combined sources3
Helixi261 – 266Combined sources6
Turni267 – 269Combined sources3
Beta strandi275 – 279Combined sources5
Helixi285 – 294Combined sources10
Beta strandi297 – 300Combined sources4
Helixi302 – 307Combined sources6
Helixi315 – 320Combined sources6
Beta strandi324 – 327Combined sources4
Turni331 – 333Combined sources3
Helixi339 – 355Combined sources17
Beta strandi358 – 361Combined sources4
Helixi365 – 372Combined sources8
Helixi374 – 379Combined sources6
Turni383 – 385Combined sources3
Beta strandi386 – 388Combined sources3
Beta strandi397 – 400Combined sources4
Helixi414 – 417Combined sources4
Beta strandi418 – 420Combined sources3
Helixi423 – 431Combined sources9
Helixi434 – 436Combined sources3
Beta strandi437 – 442Combined sources6
Beta strandi445 – 448Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UZ9X-ray2.30A1-454[»]
3E0LX-ray2.37A/B1-454[»]
4AQLX-ray1.99A1-454[»]
ProteinModelPortaliQ9Y2T3.
SMRiQ9Y2T3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y2T3.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATZ/TRZ family.Curated

Phylogenomic databases

eggNOGiKOG3968. Eukaryota.
COG0402. LUCA.
GeneTreeiENSGT00390000017130.
HOGENOMiHOG000257692.
HOVERGENiHBG005930.
InParanoidiQ9Y2T3.
KOiK01487.
OMAiDFFGDIS.
OrthoDBiEOG091G07AM.
PhylomeDBiQ9Y2T3.
TreeFamiTF324539.

Family and domain databases

CDDicd01303. GDEase. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR014311. Guanine_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11271:SF6. PTHR11271:SF6. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR02967. guan_deamin. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2T3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCAAQMPPLA HIFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEEASQ
60 70 80 90 100
QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHASQYSF AGSSIDLPLL
110 120 130 140 150
EWLTKYTFPA EHRFQNIDFA EEVYTRVVRR TLKNGTTTAC YFATIHTDSS
160 170 180 190 200
LLLADITDKF GQRAFVGKVC MDLNDTFPEY KETTEESIKE TERFVSEMLQ
210 220 230 240 250
KNYSRVKPIV TPRFSLSCSE TLMGELGNIA KTRDLHIQSH ISENRDEVEA
260 270 280 290 300
VKNLYPSYKN YTSVYDKNNL LTNKTVMAHG CYLSAEELNV FHERGASIAH
310 320 330 340 350
CPNSNLSLSS GFLNVLEVLK HEVKIGLGTD VAGGYSYSML DAIRRAVMVS
360 370 380 390 400
NILLINKVNE KSLTLKEVFR LATLGGSQAL GLDGEIGNFE VGKEFDAILI
410 420 430 440 450
NPKASDSPID LFYGDFFGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF

SSSV
Length:454
Mass (Da):51,003
Last modified:November 1, 1999 - v1
Checksum:iA45C868E6EEA7380
GO
Isoform 2 (identifier: Q9Y2T3-2) [UniParc]FASTAAdd to basket
Also known as: c

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Show »
Length:380
Mass (Da):42,445
Checksum:i6AD0F99117A467CE
GO
Isoform 3 (identifier: Q9Y2T3-3) [UniParc]FASTAAdd to basket
Also known as: a

The sequence of this isoform differs from the canonical sequence as follows:
     454-454: V → VKETIHLPASSPHPPPFP

Show »
Length:471
Mass (Da):52,837
Checksum:i5B9BF9B0E1111F88
GO

Sequence cautioni

The sequence BAA86572 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316 – 318LEV → ARI in AAG40469 (Ref. 3) Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0420751 – 74Missing in isoform 2. 2 PublicationsAdd BLAST74
Alternative sequenceiVSP_042076454V → VKETIHLPASSPHPPPFP in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095286 mRNA. Translation: AAD25978.1.
AF019638 mRNA. Translation: AAF13301.1.
AF144745 mRNA. Translation: AAG40469.1.
AB033084 mRNA. Translation: BAA86572.1. Different initiation.
AK300418 mRNA. Translation: BAG62147.1.
AK315988 mRNA. Translation: BAH14359.1.
AL583829 Genomic DNA. No translation available.
AL590311, AL135924 Genomic DNA. Translation: CAI12631.1.
AL135924, AL590311 Genomic DNA. Translation: CAI16261.1.
CH471089 Genomic DNA. Translation: EAW62529.1.
BC053584 mRNA. Translation: AAH53584.1.
CCDSiCCDS56576.1. [Q9Y2T3-3]
CCDS56577.1. [Q9Y2T3-2]
CCDS6641.1. [Q9Y2T3-1]
RefSeqiNP_001229434.1. NM_001242505.2. [Q9Y2T3-3]
NP_001229435.1. NM_001242506.2. [Q9Y2T3-2]
NP_001229436.1. NM_001242507.2. [Q9Y2T3-2]
NP_004284.1. NM_004293.4. [Q9Y2T3-1]
XP_005252374.1. XM_005252317.1. [Q9Y2T3-3]
XP_016870825.1. XM_017015336.1. [Q9Y2T3-3]
UniGeneiHs.494163.

Genome annotation databases

EnsembliENST00000238018; ENSP00000238018; ENSG00000119125. [Q9Y2T3-3]
ENST00000358399; ENSP00000351170; ENSG00000119125. [Q9Y2T3-1]
ENST00000475764; ENSP00000436619; ENSG00000119125. [Q9Y2T3-1]
ENST00000545168; ENSP00000437972; ENSG00000119125. [Q9Y2T3-2]
GeneIDi9615.
KEGGihsa:9615.
UCSCiuc004aiq.4. human. [Q9Y2T3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095286 mRNA. Translation: AAD25978.1.
AF019638 mRNA. Translation: AAF13301.1.
AF144745 mRNA. Translation: AAG40469.1.
AB033084 mRNA. Translation: BAA86572.1. Different initiation.
AK300418 mRNA. Translation: BAG62147.1.
AK315988 mRNA. Translation: BAH14359.1.
AL583829 Genomic DNA. No translation available.
AL590311, AL135924 Genomic DNA. Translation: CAI12631.1.
AL135924, AL590311 Genomic DNA. Translation: CAI16261.1.
CH471089 Genomic DNA. Translation: EAW62529.1.
BC053584 mRNA. Translation: AAH53584.1.
CCDSiCCDS56576.1. [Q9Y2T3-3]
CCDS56577.1. [Q9Y2T3-2]
CCDS6641.1. [Q9Y2T3-1]
RefSeqiNP_001229434.1. NM_001242505.2. [Q9Y2T3-3]
NP_001229435.1. NM_001242506.2. [Q9Y2T3-2]
NP_001229436.1. NM_001242507.2. [Q9Y2T3-2]
NP_004284.1. NM_004293.4. [Q9Y2T3-1]
XP_005252374.1. XM_005252317.1. [Q9Y2T3-3]
XP_016870825.1. XM_017015336.1. [Q9Y2T3-3]
UniGeneiHs.494163.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UZ9X-ray2.30A1-454[»]
3E0LX-ray2.37A/B1-454[»]
4AQLX-ray1.99A1-454[»]
ProteinModelPortaliQ9Y2T3.
SMRiQ9Y2T3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114976. 36 interactors.
MINTiMINT-109340.
STRINGi9606.ENSP00000238018.

Chemistry databases

BindingDBiQ9Y2T3.
ChEMBLiCHEMBL3129.

Protein family/group databases

MEROPSiM38.981.

PTM databases

iPTMnetiQ9Y2T3.
PhosphoSitePlusiQ9Y2T3.

Polymorphism and mutation databases

BioMutaiGDA.
DMDMi9910736.

Proteomic databases

EPDiQ9Y2T3.
PaxDbiQ9Y2T3.
PeptideAtlasiQ9Y2T3.
PRIDEiQ9Y2T3.

Protocols and materials databases

DNASUi9615.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000238018; ENSP00000238018; ENSG00000119125. [Q9Y2T3-3]
ENST00000358399; ENSP00000351170; ENSG00000119125. [Q9Y2T3-1]
ENST00000475764; ENSP00000436619; ENSG00000119125. [Q9Y2T3-1]
ENST00000545168; ENSP00000437972; ENSG00000119125. [Q9Y2T3-2]
GeneIDi9615.
KEGGihsa:9615.
UCSCiuc004aiq.4. human. [Q9Y2T3-1]

Organism-specific databases

CTDi9615.
DisGeNETi9615.
GeneCardsiGDA.
HGNCiHGNC:4212. GDA.
HPAiHPA019352.
HPA024099.
HPA030387.
MIMi139260. gene.
neXtProtiNX_Q9Y2T3.
OpenTargetsiENSG00000119125.
PharmGKBiPA28625.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3968. Eukaryota.
COG0402. LUCA.
GeneTreeiENSGT00390000017130.
HOGENOMiHOG000257692.
HOVERGENiHBG005930.
InParanoidiQ9Y2T3.
KOiK01487.
OMAiDFFGDIS.
OrthoDBiEOG091G07AM.
PhylomeDBiQ9Y2T3.
TreeFamiTF324539.

Enzyme and pathway databases

UniPathwayiUPA00603; UER00660.
BioCyciMetaCyc:HS04276-MONOMER.
ZFISH:HS04276-MONOMER.
BRENDAi3.5.4.3. 2681.
ReactomeiR-HSA-74259. Purine catabolism.
SABIO-RKQ9Y2T3.

Miscellaneous databases

ChiTaRSiGDA. human.
EvolutionaryTraceiQ9Y2T3.
GenomeRNAii9615.
PROiQ9Y2T3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000119125.
CleanExiHS_GDA.
ExpressionAtlasiQ9Y2T3. baseline and differential.
GenevisibleiQ9Y2T3. HS.

Family and domain databases

CDDicd01303. GDEase. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR014311. Guanine_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11271:SF6. PTHR11271:SF6. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR02967. guan_deamin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGUAD_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2T3
Secondary accession number(s): B4DTY5
, Q5SZC7, Q9H335, Q9ULG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.