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Protein

Zinc finger protein 256

Gene

ZNF256

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor that plays a role in cell proliferation. Requires TRIM28 for its activity.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri90 – 11223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri239 – 26123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri267 – 28923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri295 – 31723C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri323 – 34523C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri351 – 37323C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri379 – 40123C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri407 – 42923C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri435 – 45723C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri463 – 48523C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri491 – 51323C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri519 – 54123C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri547 – 56923C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri575 – 59723C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri603 – 62523C2H2-type 15PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  • multicellular organismal development Source: ProtInc
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_12627. Generic Transcription Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 256
Alternative name(s):
Bone marrow zinc finger 3
Short name:
BMZF-3
Gene namesi
Name:ZNF256
Synonyms:BMZF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:13049. ZNF256.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37627.

Polymorphism and mutation databases

BioMutaiZNF256.
DMDMi254763378.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Zinc finger protein 256PRO_0000047489Add
BLAST

Proteomic databases

PaxDbiQ9Y2P7.
PRIDEiQ9Y2P7.

PTM databases

PhosphoSiteiQ9Y2P7.

Expressioni

Inductioni

By GDNF/glial cell line-derived neurotrophic factor.1 Publication

Gene expression databases

BgeeiQ9Y2P7.
CleanExiHS_ZNF256.
ExpressionAtlasiQ9Y2P7. baseline and differential.
GenevisibleiQ9Y2P7. HS.

Organism-specific databases

HPAiHPA055390.

Interactioni

Subunit structurei

Interacts with TRIM28.1 Publication

Protein-protein interaction databases

BioGridi115474. 1 interaction.
STRINGi9606.ENSP00000282308.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2P7.
SMRiQ9Y2P7. Positions 88-626.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 9683KRABPROSITE-ProRule annotationAdd
BLAST

Domaini

The KRAB domain mediates interaction with TRIM28 and is required for transcriptional repressor activity.1 Publication

Sequence similaritiesi

Contains 15 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri90 – 11223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri239 – 26123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri267 – 28923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri295 – 31723C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri323 – 34523C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri351 – 37323C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri379 – 40123C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri407 – 42923C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri435 – 45723C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri463 – 48523C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri491 – 51323C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri519 – 54123C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri547 – 56923C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri575 – 59723C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri603 – 62523C2H2-type 15PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000119107.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ9Y2P7.
KOiK09228.
OMAiYDLLIHQ.
OrthoDBiEOG7KSX7Q.
PhylomeDBiQ9Y2P7.
TreeFamiTF339848.

Family and domain databases

Gene3Di3.30.160.60. 14 hits.
InterProiIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 15 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 15 hits.
PS50157. ZINC_FINGER_C2H2_2. 15 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2P7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAELTAPA QGIVTFEDVA VYFSWKEWGL LDEAQKCLYH DVMLENLTLT
60 70 80 90 100
TSLGGSGAGD EEAPYQQSTS PQRVSQVRIP KALPSPQKTN PCEICGPVLR
110 120 130 140 150
QILHLVEHQG THHGQKLYTD GACRKQLQFT AYLHQHQKQH VGQKHFRSNG
160 170 180 190 200
GRDMFLSSCT FEVSGKPFTC KEVGKDFLVR SRFLQQQAAH TRKKSNRTKS
210 220 230 240 250
AVAFHSVKNH YNWGECVKAF SYKHVRVQHQ GDLIRERSYM CSECGKSFST
260 270 280 290 300
SCSLSDHLRV HTSEKPYTCG ECGKSYRQSS SLITHRRIHT GVRPHQCDEC
310 320 330 340 350
GKLFNRKYDL LIHQRVHTGE RPYKCSECGK SFSHSSSLIT HQRIHTGMRP
360 370 380 390 400
YECSECGKSF IHSSSLITHQ RVHTGTRPYM CSECGKSFSQ SCHLIKHRRL
410 420 430 440 450
HIGEGPYECS ECGKLFTYRS RFFQHQRVHT GVRSHECHEC GKLFSRKFDL
460 470 480 490 500
IVHERVHTGE RPYECSECGK SFTCKSYLIS HWKVHTGARP YECGECGKSF
510 520 530 540 550
THSSTLLQHQ RVHTGERPYE CNECGKFFSQ SSSLIRHRRS HTGERPYECS
560 570 580 590 600
ECWKSFSNHS SLVKHRRVHT GERPYECSEC GKSFSQSSNL TNHQRIHSGE
610 620
RPYECSDCGK FFTFNSNLLK HQNVHKG
Length:627
Mass (Da):71,871
Last modified:July 28, 2009 - v2
Checksum:iF03506B03E4A6E86
GO
Isoform 2 (identifier: Q9Y2P7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-153: Missing.

Show »
Length:474
Mass (Da):54,856
Checksum:i304E4C25742FD014
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti254 – 2541L → P in BAG36789 (PubMed:14702039).Curated
Sequence conflicti552 – 5521C → F in BAG36789 (PubMed:14702039).Curated
Sequence conflicti574 – 5741P → L in AAD32449 (PubMed:10585455).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti304 – 3041F → I.
Corresponds to variant rs953619 [ dbSNP | Ensembl ].
VAR_058326

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 153153Missing in isoform 2. 3 PublicationsVSP_037721Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067165 mRNA. Translation: AAD32449.1.
BT006853 mRNA. Translation: AAP35499.1.
AK314094 mRNA. Translation: BAG36789.1.
AC008969 Genomic DNA. No translation available.
AC010326 Genomic DNA. No translation available.
BC001438 mRNA. Translation: AAH01438.2.
CCDSiCCDS12966.1. [Q9Y2P7-1]
RefSeqiNP_005764.2. NM_005773.2. [Q9Y2P7-1]
UniGeneiHs.596242.

Genome annotation databases

EnsembliENST00000282308; ENSP00000282308; ENSG00000152454.
GeneIDi10172.
KEGGihsa:10172.
UCSCiuc002qqu.3. human. [Q9Y2P7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067165 mRNA. Translation: AAD32449.1.
BT006853 mRNA. Translation: AAP35499.1.
AK314094 mRNA. Translation: BAG36789.1.
AC008969 Genomic DNA. No translation available.
AC010326 Genomic DNA. No translation available.
BC001438 mRNA. Translation: AAH01438.2.
CCDSiCCDS12966.1. [Q9Y2P7-1]
RefSeqiNP_005764.2. NM_005773.2. [Q9Y2P7-1]
UniGeneiHs.596242.

3D structure databases

ProteinModelPortaliQ9Y2P7.
SMRiQ9Y2P7. Positions 88-626.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115474. 1 interaction.
STRINGi9606.ENSP00000282308.

PTM databases

PhosphoSiteiQ9Y2P7.

Polymorphism and mutation databases

BioMutaiZNF256.
DMDMi254763378.

Proteomic databases

PaxDbiQ9Y2P7.
PRIDEiQ9Y2P7.

Protocols and materials databases

DNASUi10172.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282308; ENSP00000282308; ENSG00000152454.
GeneIDi10172.
KEGGihsa:10172.
UCSCiuc002qqu.3. human. [Q9Y2P7-1]

Organism-specific databases

CTDi10172.
GeneCardsiGC19M058452.
HGNCiHGNC:13049. ZNF256.
HPAiHPA055390.
MIMi606956. gene.
neXtProtiNX_Q9Y2P7.
PharmGKBiPA37627.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000119107.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ9Y2P7.
KOiK09228.
OMAiYDLLIHQ.
OrthoDBiEOG7KSX7Q.
PhylomeDBiQ9Y2P7.
TreeFamiTF339848.

Enzyme and pathway databases

ReactomeiREACT_12627. Generic Transcription Pathway.

Miscellaneous databases

GenomeRNAii10172.
NextBioi38516.
PROiQ9Y2P7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y2P7.
CleanExiHS_ZNF256.
ExpressionAtlasiQ9Y2P7. baseline and differential.
GenevisibleiQ9Y2P7. HS.

Family and domain databases

Gene3Di3.30.160.60. 14 hits.
InterProiIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 15 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 15 hits.
PS50157. ZINC_FINGER_C2H2_2. 15 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of six novel Kruppel-like zinc finger genes from hematopoietic cells and identification of a novel transregulatory domain KRNB."
    Han Z.-G., Zhang Q.-H., Ye M., Kan L.-X., Gu B.-W., He K.-L., Shi S.-L., Zhou J., Fu G., Mao M., Chen S.-J., Yu L., Chen Z.
    J. Biol. Chem. 274:35741-35748(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Bone marrow.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Tongue.
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  6. "A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor associated with KAP-1."
    Suzuki C., Murakumo Y., Kawase Y., Sato T., Morinaga T., Fukuda N., Enomoto A., Ichihara M., Takahashi M.
    Biochem. Biophys. Res. Commun. 366:226-232(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH TRIM28.

Entry informationi

Entry nameiZN256_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2P7
Secondary accession number(s): B2RA92, Q53Y85, Q9BV71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.