Q9Y2P7 (ZN256_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 256

Alternative name(s):
Bone marrow zinc finger 3
Short name=BMZF-3

Gene names

Name:	ZNF256
Synonyms:	BMZF3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor that plays a role in cell proliferation. Requires TRIM28 for its activity. Ref.6
Subunit structure	Interacts with TRIM28. Ref.6
Subcellular location	Nucleus Ref.6.
Induction	By GDNF/glial cell line-derived neurotrophic factor. Ref.6
Domain	The KRAB domain mediates interaction with TRIM28 and is required for transcriptional repressor activity. Ref.6
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 15 C2H2-type zinc fingers. Contains 1 KRAB domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Traceable author statement Ref.1. Source: ProtInc negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.6. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement Ref.1. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2P7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2P7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-153: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 627	627	Zinc finger protein 256		PRO_0000047489
Regions
	Domain	14 – 96	83	KRAB
	Zinc finger	90 – 112	23	C2H2-type 1
	Zinc finger	239 – 261	23	C2H2-type 2
	Zinc finger	267 – 289	23	C2H2-type 3
	Zinc finger	295 – 317	23	C2H2-type 4
	Zinc finger	323 – 345	23	C2H2-type 5
	Zinc finger	351 – 373	23	C2H2-type 6
	Zinc finger	379 – 401	23	C2H2-type 7
	Zinc finger	407 – 429	23	C2H2-type 8
	Zinc finger	435 – 457	23	C2H2-type 9
	Zinc finger	463 – 485	23	C2H2-type 10
	Zinc finger	491 – 513	23	C2H2-type 11
	Zinc finger	519 – 541	23	C2H2-type 12
	Zinc finger	547 – 569	23	C2H2-type 13
	Zinc finger	575 – 597	23	C2H2-type 14
	Zinc finger	603 – 625	23	C2H2-type 15
Natural variations
	Alternative sequence	1 – 153	153	Missing in isoform 2.		VSP_037721
	Natural variant	304	1	F → I. Corresponds to variant rs953619 [ dbSNP | Ensembl ].		VAR_058326
Experimental info
	Sequence conflict	254	1	L → P in BAG36789. Ref.3
	Sequence conflict	552	1	C → F in BAG36789. Ref.3
	Sequence conflict	574	1	P → L in AAD32449. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: F03506B03E4A6E86	FASTA	627	71,871
	10 20 30 40 50 60 MAAAELTAPA QGIVTFEDVA VYFSWKEWGL LDEAQKCLYH DVMLENLTLT TSLGGSGAGD 70 80 90 100 110 120 EEAPYQQSTS PQRVSQVRIP KALPSPQKTN PCEICGPVLR QILHLVEHQG THHGQKLYTD 130 140 150 160 170 180 GACRKQLQFT AYLHQHQKQH VGQKHFRSNG GRDMFLSSCT FEVSGKPFTC KEVGKDFLVR 190 200 210 220 230 240 SRFLQQQAAH TRKKSNRTKS AVAFHSVKNH YNWGECVKAF SYKHVRVQHQ GDLIRERSYM 250 260 270 280 290 300 CSECGKSFST SCSLSDHLRV HTSEKPYTCG ECGKSYRQSS SLITHRRIHT GVRPHQCDEC 310 320 330 340 350 360 GKLFNRKYDL LIHQRVHTGE RPYKCSECGK SFSHSSSLIT HQRIHTGMRP YECSECGKSF 370 380 390 400 410 420 IHSSSLITHQ RVHTGTRPYM CSECGKSFSQ SCHLIKHRRL HIGEGPYECS ECGKLFTYRS 430 440 450 460 470 480 RFFQHQRVHT GVRSHECHEC GKLFSRKFDL IVHERVHTGE RPYECSECGK SFTCKSYLIS 490 500 510 520 530 540 HWKVHTGARP YECGECGKSF THSSTLLQHQ RVHTGERPYE CNECGKFFSQ SSSLIRHRRS 550 560 570 580 590 600 HTGERPYECS ECWKSFSNHS SLVKHRRVHT GERPYECSEC GKSFSQSSNL TNHQRIHSGE 610 620 RPYECSDCGK FFTFNSNLLK HQNVHKG
	Isoform 2 [UniParc]. Checksum: 304E4C25742FD014	FASTA	474	54,856
	10 20 30 40 50 60 MFLSSCTFEV SGKPFTCKEV GKDFLVRSRF LQQQAAHTRK KSNRTKSAVA FHSVKNHYNW 70 80 90 100 110 120 GECVKAFSYK HVRVQHQGDL IRERSYMCSE CGKSFSTSCS LSDHLRVHTS EKPYTCGECG 130 140 150 160 170 180 KSYRQSSSLI THRRIHTGVR PHQCDECGKL FNRKYDLLIH QRVHTGERPY KCSECGKSFS 190 200 210 220 230 240 HSSSLITHQR IHTGMRPYEC SECGKSFIHS SSLITHQRVH TGTRPYMCSE CGKSFSQSCH 250 260 270 280 290 300 LIKHRRLHIG EGPYECSECG KLFTYRSRFF QHQRVHTGVR SHECHECGKL FSRKFDLIVH 310 320 330 340 350 360 ERVHTGERPY ECSECGKSFT CKSYLISHWK VHTGARPYEC GECGKSFTHS STLLQHQRVH 370 380 390 400 410 420 TGERPYECNE CGKFFSQSSS LIRHRRSHTG ERPYECSECW KSFSNHSSLV KHRRVHTGER 430 440 450 460 470 PYECSECGKS FSQSSNLTNH QRIHSGERPY ECSDCGKFFT FNSNLLKHQN VHKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of six novel Kruppel-like zinc finger genes from hematopoietic cells and identification of a novel transregulatory domain KRNB." Han Z.-G., Zhang Q.-H., Ye M., Kan L.-X., Gu B.-W., He K.-L., Shi S.-L., Zhou J., Fu G., Mao M., Chen S.-J., Yu L., Chen Z. J. Biol. Chem. 274:35741-35748(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Bone marrow.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Tongue.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	"A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor associated with KAP-1." Suzuki C., Murakumo Y., Kawase Y., Sato T., Morinaga T., Fukuda N., Enomoto A., Ichihara M., Takahashi M. Biochem. Biophys. Res. Commun. 366:226-232(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH TRIM28.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF067165 mRNA. Translation: AAD32449.1. BT006853 mRNA. Translation: AAP35499.1. AK314094 mRNA. Translation: BAG36789.1. AC008969 Genomic DNA. No translation available. AC010326 Genomic DNA. No translation available. BC001438 mRNA. Translation: AAH01438.2.
IPI	IPI00032392. IPI00941825.
RefSeq	NP_005764.2. NM_005773.2.
UniGene	Hs.596242.
3D structure databases
ProteinModelPortal	Q9Y2P7.
ModBase	Search...
PTM databases
PhosphoSite	Q9Y2P7.
Polymorphism databases
DMDM	254763378.
Proteomic databases
PaxDb	Q9Y2P7.
PRIDE	Q9Y2P7.
Protocols and materials databases
DNASU	10172.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282308; ENSP00000282308; ENSG00000152454.
GeneID	10172.
KEGG	hsa:10172.
UCSC	uc002qqu.3. human.
Organism-specific databases
CTD	10172.
GeneCards	GC19M058452.
HGNC	HGNC:13049. ZNF256.
HPA	HPA055390.
MIM	606956. gene.
neXtProt	NX_Q9Y2P7.
PharmGKB	PA37627.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000234617.
HOVERGEN	HBG018163.
InParanoid	Q9Y2P7.
KO	K09228.
OMA	HTGVRSH.
OrthoDB	EOG4JHCF4.
PhylomeDB	Q9Y2P7.
Enzyme and pathway databases
Reactome	REACT_71. Gene Expression.
Gene expression databases
Bgee	Q9Y2P7.
CleanEx	HS_ZNF256.
Genevestigator	Q9Y2P7.
GermOnline	ENSG00000152454. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 14 hits.
InterPro	IPR001909. Krueppel-associated_box. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF01352. KRAB. 1 hit. [Graphical view]
SMART	SM00349. KRAB. 1 hit. SM00355. ZnF_C2H2. 15 hits. [Graphical view]
SUPFAM	SSF109640. Krueppel-associated_box. 1 hit.
PROSITE	PS50805. KRAB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 15 hits. PS50157. ZINC_FINGER_C2H2_2. 15 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10172.
NextBio	38516.
SOURCE	Search...

Entry information

Entry name

ZN256_HUMAN

Accession

Primary (citable) accession number: Q9Y2P7
Secondary accession number(s): B2RA92, Q53Y85, Q9BV71

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.