Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bile acyl-CoA synthetase

Gene

SLC27A5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.2 Publications

Catalytic activityi

ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.
ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi292 – 303AMPSequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-159418 Recycling of bile acids and salts
R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol

Protein family/group databases

TCDBi4.C.1.1.13 the fatty acid transporter (fat) family

Chemistry databases

SwissLipidsiSLP:000000429
SLP:000001315 [Q9Y2P5-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Bile acyl-CoA synthetase (EC:6.2.1.7)
Short name:
BACS
Alternative name(s):
Bile acid-CoA ligase
Short name:
BA-CoA ligase
Short name:
BAL
Cholate--CoA ligase
Fatty acid transport protein 5
Short name:
FATP-5
Fatty-acid-coenzyme A ligase, very long-chain 3
Solute carrier family 27 member 5
Very long-chain acyl-CoA synthetase homolog 2
Short name:
VLCS-H2
Short name:
VLCSH2
Very long-chain acyl-CoA synthetase-related protein
Short name:
VLACS-related
Short name:
VLACSR
Gene namesi
Name:SLC27A5
Synonyms:ACSB, ACSVL6, FACVL3, FATP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000083807.9
HGNCiHGNC:10999 SLC27A5
MIMi603314 gene
neXtProtiNX_Q9Y2P5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30Cytoplasmic1 PublicationAdd BLAST30
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Transmembranei56 – 76HelicalSequence analysisAdd BLAST21
Topological domaini77 – 690Cytoplasmic1 PublicationAdd BLAST614

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10998
MalaCardsiSLC27A5
OpenTargetsiENSG00000083807
PharmGKBiPA35873

Chemistry databases

ChEMBLiCHEMBL5196

Polymorphism and mutation databases

BioMutaiSLC27A5
DMDMi74739456

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932131 – 690Bile acyl-CoA synthetaseAdd BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei501PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Y2P5
PeptideAtlasiQ9Y2P5
PRIDEiQ9Y2P5

PTM databases

iPTMnetiQ9Y2P5
PhosphoSitePlusiQ9Y2P5

Expressioni

Tissue specificityi

Predominantly expressed in liver.1 Publication

Gene expression databases

BgeeiENSG00000083807
CleanExiHS_SLC27A5
ExpressionAtlasiQ9Y2P5 baseline and differential
GenevisibleiQ9Y2P5 HS

Organism-specific databases

HPAiCAB056160
CAB068239
HPA007292

Interactioni

Protein-protein interaction databases

BioGridi116191, 1 interactor
IntActiQ9Y2P5, 1 interactor
STRINGi9606.ENSP00000263093

Structurei

3D structure databases

ProteinModelPortaliQ9Y2P5
SMRiQ9Y2P5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179 Eukaryota
ENOG410XQ8T LUCA
GeneTreeiENSGT00550000074420
HOGENOMiHOG000044189
HOVERGENiHBG005642
InParanoidiQ9Y2P5
KOiK08748
OMAiDCRQHGV
OrthoDBiEOG091G0B76
PhylomeDBiQ9Y2P5
TreeFamiTF313430

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR030302 FATP5
PANTHERiPTHR43107:SF4 PTHR43107:SF4, 1 hit
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2P5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVRQQLALL LLLLLLLWGL GQPVWPVAVA LTLRWLLGDP TCCVLLGLAM
60 70 80 90 100
LARPWLGPWV PHGLSLAAAA LALTLLPARL PPGLRWLPAD VIFLAKILHL
110 120 130 140 150
GLKIRGCLSR QPPDTFVDAF ERRARAQPGR ALLVWTGPGA GSVTFGELDA
160 170 180 190 200
RACQAAWALK AELGDPASLC AGEPTALLVL ASQAVPALCM WLGLAKLGCP
210 220 230 240 250
TAWINPHGRG MPLAHSVLSS GARVLVVDPD LRESLEEILP KLQAENIRCF
260 270 280 290 300
YLSHTSPTPG VGALGAALDA APSHPVPADL RAGITWRSPA LFIYTSGTTG
310 320 330 340 350
LPKPAILTHE RVLQMSKMLS LSGATADDVV YTVLPLYHVM GLVVGILGCL
360 370 380 390 400
DLGATCVLAP KFSTSCFWDD CRQHGVTVIL YVGELLRYLC NIPQQPEDRT
410 420 430 440 450
HTVRLAMGNG LRADVWETFQ QRFGPIRIWE VYGSTEGNMG LVNYVGRCGA
460 470 480 490 500
LGKMSCLLRM LSPFELVQFD MEAAEPVRDN QGFCIPVGLG EPGLLLTKVV
510 520 530 540 550
SQQPFVGYRG PRELSERKLV RNVRQSGDVY YNTGDVLAMD REGFLYFRDR
560 570 580 590 600
LGDTFRWKGE NVSTHEVEGV LSQVDFLQQV NVYGVCVPGC EGKVGMAAVQ
610 620 630 640 650
LAPGQTFDGE KLYQHVRAWL PAYATPHFIR IQDAMEVTST FKLMKTRLVR
660 670 680 690
EGFNVGIVVD PLFVLDNRAQ SFRPLTAEMY QAVCEGTWRL
Length:690
Mass (Da):75,385
Last modified:November 1, 1999 - v1
Checksum:i011313424D794546
GO
Isoform 2 (identifier: Q9Y2P5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-229: Missing.

Note: No experimental confirmation available.
Show »
Length:606
Mass (Da):66,846
Checksum:i4A49B281561FF8D6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04824350M → T. Corresponds to variant dbSNP:rs35350976EnsemblClinVar.1
Natural variantiVAR_04824453R → W. Corresponds to variant dbSNP:rs34415062EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055810146 – 229Missing in isoform 2. 1 PublicationAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064255 mRNA Translation: AAD29444.1
AK123036 mRNA Translation: BAG53858.1
AK298446 mRNA Translation: BAG60663.1
AC012313 Genomic DNA No translation available.
CH471135 Genomic DNA Translation: EAW72601.1
CCDSiCCDS12983.1 [Q9Y2P5-1]
CCDS82415.1 [Q9Y2P5-2]
RefSeqiNP_001308125.1, NM_001321196.1 [Q9Y2P5-2]
NP_036386.1, NM_012254.2 [Q9Y2P5-1]
UniGeneiHs.292177

Genome annotation databases

EnsembliENST00000263093; ENSP00000263093; ENSG00000083807 [Q9Y2P5-1]
ENST00000601355; ENSP00000470368; ENSG00000083807 [Q9Y2P5-2]
GeneIDi10998
KEGGihsa:10998
UCSCiuc002qtc.3 human [Q9Y2P5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiS27A5_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2P5
Secondary accession number(s): B3KVP6, B4DPQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health