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Protein

Rabphilin-3A

Gene

RPH3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi422Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi423Calcium 1By similarity1
Metal bindingi423Calcium 2By similarity1
Metal bindingi429Calcium 2By similarity1
Metal bindingi484Calcium 1By similarity1
Metal bindingi484Calcium 2By similarity1
Metal bindingi485Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi486Calcium 1By similarity1
Metal bindingi486Calcium 2By similarity1
Metal bindingi492Calcium 1By similarity1
Metal bindingi539Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi581Calcium 3By similarity1
Metal bindingi581Calcium 4By similarity1
Metal bindingi587Calcium 3By similarity1
Metal bindingi641Calcium 3By similarity1
Metal bindingi641Calcium 4By similarity1
Metal bindingi642Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi643Calcium 3By similarity1
Metal bindingi643Calcium 4By similarity1
Metal bindingi649Calcium 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri92 – 148FYVE-typePROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000089169-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Rabphilin-3A
Alternative name(s):
Exophilin-1
Gene namesi
Name:RPH3A
Synonyms:KIAA0985
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:17056. RPH3A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi22895.
OpenTargetsiENSG00000089169.
PharmGKBiPA134886118.

Polymorphism and mutation databases

BioMutaiRPH3A.
DMDMi13878745.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902271 – 694Rabphilin-3AAdd BLAST694

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226Omega-N-methylarginineBy similarity1
Modified residuei272PhosphoserineBy similarity1
Modified residuei692PhosphoserineBy similarity1
Modified residuei693PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Y2J0.
PeptideAtlasiQ9Y2J0.
PRIDEiQ9Y2J0.

PTM databases

iPTMnetiQ9Y2J0.
PhosphoSitePlusiQ9Y2J0.
SwissPalmiQ9Y2J0.

Expressioni

Gene expression databases

BgeeiENSG00000089169.
CleanExiHS_RPH3A.
ExpressionAtlasiQ9Y2J0. baseline and differential.
GenevisibleiQ9Y2J0. HS.

Organism-specific databases

HPAiHPA002475.

Interactioni

Subunit structurei

Monomer. Interacts with RAB3A, RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CASKO149363EBI-1216802,EBI-1215506

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116560. 15 interactors.
IntActiQ9Y2J0. 2 interactors.
MINTiMINT-199741.
STRINGi9606.ENSP00000374036.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2J0.
SMRiQ9Y2J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 160RabBDPROSITE-ProRule annotationAdd BLAST117
Domaini394 – 498C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini552 – 655C2 2PROSITE-ProRule annotationAdd BLAST104

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 375Pro-richAdd BLAST214

Domaini

Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers (By similarity).By similarity

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri92 – 148FYVE-typePROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000294226.
HOVERGENiHBG017739.
InParanoidiQ9Y2J0.
KOiK19938.
OMAiMLGSACV.
OrthoDBiEOG091G0FPP.
PhylomeDBiQ9Y2J0.
TreeFamiTF351844.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028698. RPH3A.
IPR001565. Synaptotagmin.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF118. PTHR10024:SF118. 1 hit.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2J0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDTVFSNSS NRWMYPSDRP LQSNDKEQLQ AGWSVHPGGQ PDRQRKQEEL
60 70 80 90 100
TDEEKEIINR VIARAEKMEE MEQERIGRLV DRLENMRKNV AGDGVNRCIL
110 120 130 140 150
CGEQLGMLGS ACVVCEDCKK NVCTKCGVET NNRLHSVWLC KICIEQREVW
160 170 180 190 200
KRSGAWFFKG FPKQVLPQPM PIKKTKPQQP VSEPAAPEQP APEPKHPARA
210 220 230 240 250
PARGDSEDRR GPGQKTGPDP ASAPGRGNYG PPVRRASEAR MSSSSRDSES
260 270 280 290 300
WDHSGGAGDS SRSPAGLRRA NSVQASRPAP GSVQSPAPPQ PGQPGTPGGS
310 320 330 340 350
RPGPGPAGRF PDQKPEVAPS DPGTTAPPRE ERTGGVGGYP AVGAREDRMS
360 370 380 390 400
HPSGPYSQAS AAAPQPAAAR QPPPPEEEEE EANSYDSDEA TTLGALEFSL
410 420 430 440 450
LYDQDNSSLQ CTIIKAKGLK PMDSNGLADP YVKLHLLPGA SKSNKLRTKT
460 470 480 490 500
LRNTRNPIWN ETLVYHGITD EDMQRKTLRI SVCDEDKFGH NEFIGETRFS
510 520 530 540 550
LKKLKPNQRK NFNICLERVI PMKRAGTTGS ARGMALYEEE QVERVGDIEE
560 570 580 590 600
RGKILVSLMY STQQGGLIVG IIRCVHLAAM DANGYSDPFV KLWLKPDMGK
610 620 630 640 650
KAKHKTQIKK KTLNPEFNEE FFYDIKHSDL AKKSLDISVW DYDIGKSNDY
660 670 680 690
IGGCQLGISA KGERLKHWYE CLKNKDKKIE RWHQLQNENH VSSD
Length:694
Mass (Da):76,872
Last modified:November 1, 1999 - v1
Checksum:iBD9C43F306A04D69
GO
Isoform 2 (identifier: Q9Y2J0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     24-28: NDKEQ → K

Show »
Length:690
Mass (Da):76,386
Checksum:i919E8AF287D0EBBA
GO

Sequence cautioni

The sequence BAA76829 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02101624 – 28NDKEQ → K in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023202 mRNA. Translation: BAA76829.2. Different initiation.
AK295696 mRNA. Translation: BAH12159.1.
BC017259 mRNA. Translation: AAH17259.1.
CCDSiCCDS31904.1. [Q9Y2J0-2]
CCDS44979.1. [Q9Y2J0-1]
RefSeqiNP_001137326.1. NM_001143854.1. [Q9Y2J0-1]
NP_055769.2. NM_014954.3. [Q9Y2J0-2]
XP_011536350.1. XM_011538048.1. [Q9Y2J0-1]
UniGeneiHs.21239.

Genome annotation databases

EnsembliENST00000389385; ENSP00000374036; ENSG00000089169. [Q9Y2J0-1]
ENST00000415485; ENSP00000405357; ENSG00000089169. [Q9Y2J0-1]
ENST00000543106; ENSP00000440384; ENSG00000089169. [Q9Y2J0-1]
ENST00000551052; ENSP00000448297; ENSG00000089169. [Q9Y2J0-2]
GeneIDi22895.
KEGGihsa:22895.
UCSCiuc001tty.4. human. [Q9Y2J0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023202 mRNA. Translation: BAA76829.2. Different initiation.
AK295696 mRNA. Translation: BAH12159.1.
BC017259 mRNA. Translation: AAH17259.1.
CCDSiCCDS31904.1. [Q9Y2J0-2]
CCDS44979.1. [Q9Y2J0-1]
RefSeqiNP_001137326.1. NM_001143854.1. [Q9Y2J0-1]
NP_055769.2. NM_014954.3. [Q9Y2J0-2]
XP_011536350.1. XM_011538048.1. [Q9Y2J0-1]
UniGeneiHs.21239.

3D structure databases

ProteinModelPortaliQ9Y2J0.
SMRiQ9Y2J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116560. 15 interactors.
IntActiQ9Y2J0. 2 interactors.
MINTiMINT-199741.
STRINGi9606.ENSP00000374036.

PTM databases

iPTMnetiQ9Y2J0.
PhosphoSitePlusiQ9Y2J0.
SwissPalmiQ9Y2J0.

Polymorphism and mutation databases

BioMutaiRPH3A.
DMDMi13878745.

Proteomic databases

PaxDbiQ9Y2J0.
PeptideAtlasiQ9Y2J0.
PRIDEiQ9Y2J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000389385; ENSP00000374036; ENSG00000089169. [Q9Y2J0-1]
ENST00000415485; ENSP00000405357; ENSG00000089169. [Q9Y2J0-1]
ENST00000543106; ENSP00000440384; ENSG00000089169. [Q9Y2J0-1]
ENST00000551052; ENSP00000448297; ENSG00000089169. [Q9Y2J0-2]
GeneIDi22895.
KEGGihsa:22895.
UCSCiuc001tty.4. human. [Q9Y2J0-1]

Organism-specific databases

CTDi22895.
DisGeNETi22895.
GeneCardsiRPH3A.
HGNCiHGNC:17056. RPH3A.
HPAiHPA002475.
MIMi612159. gene.
neXtProtiNX_Q9Y2J0.
OpenTargetsiENSG00000089169.
PharmGKBiPA134886118.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000294226.
HOVERGENiHBG017739.
InParanoidiQ9Y2J0.
KOiK19938.
OMAiMLGSACV.
OrthoDBiEOG091G0FPP.
PhylomeDBiQ9Y2J0.
TreeFamiTF351844.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000089169-MONOMER.

Miscellaneous databases

ChiTaRSiRPH3A. human.
GeneWikiiRPH3A.
GenomeRNAii22895.
PROiQ9Y2J0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000089169.
CleanExiHS_RPH3A.
ExpressionAtlasiQ9Y2J0. baseline and differential.
GenevisibleiQ9Y2J0. HS.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028698. RPH3A.
IPR001565. Synaptotagmin.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF118. PTHR10024:SF118. 1 hit.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRP3A_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2J0
Secondary accession number(s): B7Z3C3, Q96AE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.