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Q9Y2I2 (NTNG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Netrin-G1
Alternative name(s):
Laminet-1
Gene names
Name:NTNG1
Synonyms:KIAA0976, LMNT1
ORF Names:UNQ571/PRO1133
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length539 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites. Ref.9

Subunit structure

Interacts with NGL1. Ref.7

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor; Extracellular side Ref.9.

Tissue specificity

Highly expressed in the thalamus, with very low expression, if any, in other tissues. Ref.7 Ref.8

Domain

The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4C/NGL1 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains.

Post-translational modification

N-glycosylated By similarity. UniProtKB Q8R4G0

Sequence similarities

Contains 3 laminin EGF-like domains.

Contains 1 laminin N-terminal domain.

Sequence caution

The sequence BAA76820.2 differs from that shown. Reason: Erroneous initiation.

Binary interactions

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 3 (identifier: Q9Y2I2-3)

Also known as: 1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Mostly expressed in adult brain.
Isoform 2 (identifier: Q9Y2I2-2)

Also known as: 1F;

The sequence of this isoform differs from the canonical sequence as follows:
     363-364: NC → SK
     365-539: Missing.
Isoform 1 (identifier: Q9Y2I2-1)

Also known as: 1C;

The sequence of this isoform differs from the canonical sequence as follows:
     363-463: Missing.
     464-464: P → T
Note: Hi expression in Expressed in brain and.
Isoform 4 (identifier: Q9Y2I2-4)

Also known as: 1D;

The sequence of this isoform differs from the canonical sequence as follows:
     364-464: CECFGHSNRC...GNSWHYGCQP → PPKFNRIWPN...SVQVANHKRA
Note: Mostly expressed in kidney, also expressed in adult and fetal brain.
Isoform 5 (identifier: Q9Y2I2-5)

Also known as: 1E;

The sequence of this isoform differs from the canonical sequence as follows:
     364-385: CECFGHSNRCSYIDLLNTVICV → PPKFNRIWPNISSLEVSNPKQA
     386-464: Missing.
Note: Some expression in fetal brain.
Isoform 6 (identifier: Q9Y2I2-6)

Also known as: 1G;

The sequence of this isoform differs from the canonical sequence as follows:
     419-463: Missing.
     464-464: P → A
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.6
Chain29 – 510482Netrin-G1
PRO_0000017091
Propeptide511 – 53929Removed in mature form Potential
PRO_0000017092

Regions

Domain46 – 296251Laminin N-terminal
Domain297 – 35660Laminin EGF-like 1
Domain364 – 41956Laminin EGF-like 2
Domain420 – 46950Laminin EGF-like 3
Region80 – 9112NGL discriminant loop I
Region208 – 2147NGL discriminant loop II
Region273 – 2753NGL discriminant loop III

Amino acid modifications

Lipidation5101GPI-anchor amidated serine Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation3201N-linked (GlcNAc...) Potential
Glycosylation4061N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 50 By similarity
Disulfide bond72 ↔ 92 By similarity
Disulfide bond80 ↔ 88
Disulfide bond182 ↔ 206 By similarity
Disulfide bond297 ↔ 306 By similarity
Disulfide bond299 ↔ 315 By similarity
Disulfide bond317 ↔ 326 By similarity
Disulfide bond329 ↔ 354 By similarity
Disulfide bond364 ↔ 373 By similarity
Disulfide bond366 ↔ 384 By similarity
Disulfide bond387 ↔ 396 By similarity
Disulfide bond399 ↔ 417 By similarity
Disulfide bond420 ↔ 432 By similarity
Disulfide bond422 ↔ 438 By similarity
Disulfide bond440 ↔ 449 By similarity
Disulfide bond452 ↔ 462 By similarity
Disulfide bond467 ↔ 480 By similarity
Disulfide bond474 ↔ 486 By similarity
Disulfide bond488 ↔ 497 By similarity

Natural variations

Alternative sequence363 – 463101Missing in isoform 1.
VSP_012574
Alternative sequence363 – 3642NC → SK in isoform 2.
VSP_010429
Alternative sequence364 – 464101CECFG…YGCQP → PPKFNRIWPNISSLEVSNPK QVAPKLALSTVSSVQVANHK RA in isoform 4.
VSP_012575
Alternative sequence364 – 38522CECFG…TVICV → PPKFNRIWPNISSLEVSNPK QA in isoform 5.
VSP_012576
Alternative sequence365 – 539175Missing in isoform 2.
VSP_010430
Alternative sequence386 – 46479Missing in isoform 5.
VSP_012577
Alternative sequence419 – 46345Missing in isoform 6.
VSP_012578
Alternative sequence4641P → T in isoform 1.
VSP_012579
Alternative sequence4641P → A in isoform 6.
VSP_012580

Experimental info

Sequence conflict61F → S in AAQ88731. Ref.2
Sequence conflict2311F → L in AAQ88731. Ref.2
Sequence conflict5211S → T in AAQ88731. Ref.2
Isoform 2:
Sequence conflict3641K → KQ in CAD98143. Ref.3

Secondary structure

........................................................... 539
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 3 (1A) [UniParc].

Last modified January 4, 2005. Version 3.
Checksum: C51F872D7A2C60A6

FASTA53960,541
        10         20         30         40         50         60 
MYLSRFLSIH ALWVTVSSVM QPYPLVWGHY DLCKTQIYTE EGKVWDYMAC QPESTDMTKY 

        70         80         90        100        110        120 
LKVKLDPPDI TCGDPPETFC AMGNPYMCNN ECDASTPELA HPPELMFDFE GRHPSTFWQS 

       130        140        150        160        170        180 
ATWKEYPKPL QVNITLSWSK TIELTDNIVI TFESGRPDQM ILEKSLDYGR TWQPYQYYAT 

       190        200        210        220        230        240 
DCLDAFHMDP KSVKDLSQHT VLEIICTEEY STGYTTNSKI IHFEIKDRFA FFAGPRLRNM 

       250        260        270        280        290        300 
ASLYGQLDTT KKLRDFFTVT DLRIRLLRPA VGEIFVDELH LARYFYAISD IKVRGRCKCN 

       310        320        330        340        350        360 
LHATVCVYDN SKLTCECEHN TTGPDCGKCK KNYQGRPWSP GSYLPIPKGT ANTCIPSISS 

       370        380        390        400        410        420 
IGNCECFGHS NRCSYIDLLN TVICVSCKHN TRGQHCELCR LGYFRNASAQ LDDENVCIEC 

       430        440        450        460        470        480 
YCNPLGSIHD RCNGSGFCEC KTGTTGPKCD ECLPGNSWHY GCQPNVCDNE LLHCQNGGTC 

       490        500        510        520        530 
HNNVRCLCPA AYTGILCEKL RCEEAGSCGS DSGQGAPPHG SPALLLLTTL LGTASPLVF 

« Hide

Isoform 2 (1F) [UniParc].

Checksum: B23DAF4BEDE9271C
Show »

FASTA36441,759
Isoform 1 (1C) [UniParc].

Checksum: BBBA6167FAC8DD71
Show »

FASTA43849,340
Isoform 4 (1D) [UniParc].

Checksum: 9099C3CF4D3F7B86
Show »

FASTA48053,946
Isoform 5 (1E) [UniParc].

Checksum: 8217255F4C8C4C65
Show »

FASTA46051,858
Isoform 6 (1G) [UniParc].

Checksum: F6783893CC0FE0D8
Show »

FASTA49455,660

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Cerebellum.
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 2; 3; 4; 5 AND 6).
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[6]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-43.
[7]"The netrin-G1 ligand NGL-1 promotes the outgrowth of thalamocortical axons."
Lin J.C., Ho W.-H., Gurney A.L., Rosenthal A.
Nat. Neurosci. 6:1270-1276(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NGL1, TISSUE SPECIFICITY.
[8]"Human netrin-G1 isoforms show evidence of differential expression."
Meerabux J.M., Ohba H., Fukasawa M., Suto Y., Aoki-Suzuki M., Nakashiba T., Nishimura S., Itohara S., Yoshikawa T.
Genomics 86:112-116(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 3; 4 AND 5), TISSUE SPECIFICITY.
[9]"Structural basis for cell surface patterning through NetrinG-NGL interactions."
Seiradake E., Coles C.H., Perestenko P.V., Harlos K., McIlhinney R.A., Aricescu A.R., Jones E.Y.
EMBO J. 30:4479-4488(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 1-520 IN COMPLEX WITH LRRC4C/NGL1, FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023193 mRNA. Translation: BAA76820.2. Different initiation.
AY358365 mRNA. Translation: AAQ88731.1.
BX538348 mRNA. Translation: CAD98143.1.
AL590427, AL513187 Genomic DNA. Translation: CAH73380.1.
AL590427, AC114491, AL513187 Genomic DNA. Translation: CAH73381.1.
AL590427, AC114491, AL513187 Genomic DNA. Translation: CAH73382.1.
AL590427, AC114491, AL513187 Genomic DNA. Translation: CAH73383.1.
AL590427, AC114491, AL513187 Genomic DNA. Translation: CAH73384.1.
AL590427, AC114491, AL513187 Genomic DNA. Translation: CAH73385.1.
AL513187, AL590427 Genomic DNA. Translation: CAH73828.1.
AL513187, AC114491, AL590427 Genomic DNA. Translation: CAH73829.1.
AL513187, AC114491, AL590427 Genomic DNA. Translation: CAH73830.1.
AL513187, AC114491, AL590427 Genomic DNA. Translation: CAH73831.1.
AL513187, AC114491, AL590427 Genomic DNA. Translation: CAH73832.1.
AL513187, AC114491, AL590427 Genomic DNA. Translation: CAH73833.1.
BC030220 mRNA. Translation: AAH30220.1.
RefSeqNP_001106697.1. NM_001113226.1.
NP_001106699.1. NM_001113228.1.
NP_055732.2. NM_014917.2.
UniGeneHs.732535.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZYJX-ray3.25B/D1-520[»]
ProteinModelPortalQ9Y2I2.
SMRQ9Y2I2. Positions 30-533.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116525. 1 interaction.
IntActQ9Y2I2. 2 interactions.
MINTMINT-8300827.
STRING9606.ENSP00000359085.

PTM databases

PhosphoSiteQ9Y2I2.

Polymorphism databases

DMDM57015420.

Proteomic databases

PaxDbQ9Y2I2.
PRIDEQ9Y2I2.

Protocols and materials databases

DNASU22854.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370065; ENSP00000359082; ENSG00000162631. [Q9Y2I2-6]
ENST00000370066; ENSP00000359083; ENSG00000162631. [Q9Y2I2-4]
ENST00000370067; ENSP00000359084; ENSG00000162631. [Q9Y2I2-5]
ENST00000370068; ENSP00000359085; ENSG00000162631. [Q9Y2I2-3]
ENST00000370071; ENSP00000359088; ENSG00000162631. [Q9Y2I2-4]
ENST00000370073; ENSP00000359090; ENSG00000162631. [Q9Y2I2-3]
ENST00000370074; ENSP00000359091; ENSG00000162631. [Q9Y2I2-1]
GeneID22854.
KEGGhsa:22854.
UCSCuc001dvc.4. human. [Q9Y2I2-1]
uc001dvd.1. human. [Q9Y2I2-2]
uc001dvf.4. human. [Q9Y2I2-4]
uc001dvh.4. human. [Q9Y2I2-3]

Organism-specific databases

CTD22854.
GeneCardsGC01P107682.
HGNCHGNC:23319. NTNG1.
MIM608818. gene.
neXtProtNX_Q9Y2I2.
Orphanet3095. Atypical Rett syndrome.
PharmGKBPA164742200.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG286598.
HOVERGENHBG052676.
KOK07522.
OMAYCECFGH.
OrthoDBEOG7HQN7W.
PhylomeDBQ9Y2I2.
TreeFamTF333945.

Gene expression databases

ArrayExpressQ9Y2I2.
BgeeQ9Y2I2.
CleanExHS_NTNG1.
GenevestigatorQ9Y2I2.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002049. EGF_laminin.
IPR008211. Laminin_N.
[Graphical view]
PfamPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 3 hits.
PS50026. EGF_3. 1 hit.
PS01248. EGF_LAM_1. 1 hit.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNTNG1. human.
GeneWikiNTNG1.
GenomeRNAi22854.
NextBio43337.
PROQ9Y2I2.
SOURCESearch...

Entry information

Entry nameNTNG1_HUMAN
AccessionPrimary (citable) accession number: Q9Y2I2
Secondary accession number(s): Q5VU86 expand/collapse secondary AC list , Q5VU87, Q5VU89, Q5VU90, Q5VU91, Q7Z2Y3, Q8N633
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: January 4, 2005
Last modified: April 16, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM