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Protein

Netrin-G1

Gene

NTNG1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.1 Publication

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-G1
Alternative name(s):
Laminet-1
Gene namesi
Name:NTNG1
Synonyms:KIAA0976, LMNT1
ORF Names:UNQ571/PRO1133
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162631.18
HGNCiHGNC:23319 NTNG1
MIMi608818 gene
neXtProtiNX_Q9Y2I2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi22854
MalaCardsiNTNG1
OpenTargetsiENSG00000162631
Orphaneti3095 Atypical Rett syndrome
PharmGKBiPA164742200

Polymorphism and mutation databases

BioMutaiNTNG1
DMDMi57015420

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 281 PublicationAdd BLAST28
ChainiPRO_000001709129 – 510Netrin-G1Add BLAST482
PropeptideiPRO_0000017092511 – 539Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 50By similarity
Disulfide bondi72 ↔ 92By similarity
Disulfide bondi80 ↔ 88
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi182 ↔ 206By similarity
Disulfide bondi297 ↔ 306By similarity
Disulfide bondi299 ↔ 315By similarity
Disulfide bondi317 ↔ 326By similarity
Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi329 ↔ 354By similarity
Disulfide bondi364 ↔ 373By similarity
Disulfide bondi366 ↔ 384By similarity
Disulfide bondi387 ↔ 396By similarity
Disulfide bondi399 ↔ 417By similarity
Glycosylationi406N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi420 ↔ 432By similarity
Disulfide bondi422 ↔ 438By similarity
Glycosylationi433N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi440 ↔ 449By similarity
Disulfide bondi452 ↔ 462By similarity
Disulfide bondi467 ↔ 480By similarity
Disulfide bondi474 ↔ 486By similarity
Disulfide bondi488 ↔ 497By similarity
Lipidationi510GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9Y2I2
PeptideAtlasiQ9Y2I2
PRIDEiQ9Y2I2

PTM databases

iPTMnetiQ9Y2I2
PhosphoSitePlusiQ9Y2I2

Expressioni

Tissue specificityi

Highly expressed in the thalamus, with very low expression, if any, in other tissues.2 Publications

Gene expression databases

BgeeiENSG00000162631
CleanExiHS_NTNG1
ExpressionAtlasiQ9Y2I2 baseline and differential
GenevisibleiQ9Y2I2 HS

Organism-specific databases

HPAiHPA065954

Interactioni

Subunit structurei

Interacts with NGL1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi116525, 3 interactors
IntActiQ9Y2I2, 2 interactors
MINTiQ9Y2I2
STRINGi9606.ENSP00000359085

Structurei

Secondary structure

1539
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 36Combined sources4
Beta strandi45 – 48Combined sources4
Helixi57 – 59Combined sources3
Beta strandi62 – 67Combined sources6
Helixi68 – 70Combined sources3
Beta strandi94 – 96Combined sources3
Turni97 – 99Combined sources3
Helixi103 – 107Combined sources5
Beta strandi118 – 120Combined sources3
Beta strandi132 – 144Combined sources13
Beta strandi148 – 154Combined sources7
Beta strandi158 – 167Combined sources10
Beta strandi173 – 181Combined sources9
Helixi182 – 185Combined sources4
Helixi193 – 195Combined sources3
Turni209 – 211Combined sources3
Beta strandi213 – 215Combined sources3
Turni216 – 219Combined sources4
Beta strandi220 – 223Combined sources4
Helixi226 – 233Combined sources8
Helixi240 – 249Combined sources10
Helixi251 – 256Combined sources6
Beta strandi257 – 268Combined sources12
Beta strandi272 – 275Combined sources4
Helixi281 – 283Combined sources3
Beta strandi287 – 292Combined sources6
Beta strandi294 – 297Combined sources4
Beta strandi300 – 302Combined sources3
Beta strandi306 – 309Combined sources4
Beta strandi312 – 315Combined sources4
Beta strandi321 – 326Combined sources6
Beta strandi345 – 348Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZYJX-ray3.25B/D1-520[»]
ProteinModelPortaliQ9Y2I2
SMRiQ9Y2I2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 296Laminin N-terminalPROSITE-ProRule annotationAdd BLAST251
Domaini297 – 356Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST60
Domaini364 – 419Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST56
Domaini420 – 469Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 91NGL discriminant loop IAdd BLAST12
Regioni208 – 214NGL discriminant loop II7
Regioni273 – 275NGL discriminant loop III3

Domaini

The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4C/NGL1 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains.

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG1836 Eukaryota
KOG3512 Eukaryota
ENOG410XS7U LUCA
GeneTreeiENSGT00890000139348
HOVERGENiHBG052676
InParanoidiQ9Y2I2
KOiK07522
OMAiYCECFGH
OrthoDBiEOG091G05AV
PhylomeDBiQ9Y2I2
TreeFamiTF333945

Family and domain databases

Gene3Di2.60.120.1490, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR002049 Laminin_EGF
IPR008211 Laminin_N
IPR038684 Laminin_N_sf
PfamiView protein in Pfam
PF00053 Laminin_EGF, 3 hits
PF00055 Laminin_N, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00180 EGF_Lam, 3 hits
SM00136 LamNT, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 3 hits
PS50026 EGF_3, 1 hit
PS01248 EGF_LAM_1, 1 hit
PS50027 EGF_LAM_2, 3 hits
PS51117 LAMININ_NTER, 1 hit

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q9Y2I2-3) [UniParc]FASTAAdd to basket
Also known as: 1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYLSRFLSIH ALWVTVSSVM QPYPLVWGHY DLCKTQIYTE EGKVWDYMAC
60 70 80 90 100
QPESTDMTKY LKVKLDPPDI TCGDPPETFC AMGNPYMCNN ECDASTPELA
110 120 130 140 150
HPPELMFDFE GRHPSTFWQS ATWKEYPKPL QVNITLSWSK TIELTDNIVI
160 170 180 190 200
TFESGRPDQM ILEKSLDYGR TWQPYQYYAT DCLDAFHMDP KSVKDLSQHT
210 220 230 240 250
VLEIICTEEY STGYTTNSKI IHFEIKDRFA FFAGPRLRNM ASLYGQLDTT
260 270 280 290 300
KKLRDFFTVT DLRIRLLRPA VGEIFVDELH LARYFYAISD IKVRGRCKCN
310 320 330 340 350
LHATVCVYDN SKLTCECEHN TTGPDCGKCK KNYQGRPWSP GSYLPIPKGT
360 370 380 390 400
ANTCIPSISS IGNCECFGHS NRCSYIDLLN TVICVSCKHN TRGQHCELCR
410 420 430 440 450
LGYFRNASAQ LDDENVCIEC YCNPLGSIHD RCNGSGFCEC KTGTTGPKCD
460 470 480 490 500
ECLPGNSWHY GCQPNVCDNE LLHCQNGGTC HNNVRCLCPA AYTGILCEKL
510 520 530
RCEEAGSCGS DSGQGAPPHG SPALLLLTTL LGTASPLVF
Note: Mostly expressed in adult brain.
Length:539
Mass (Da):60,541
Last modified:January 4, 2005 - v3
Checksum:iC51F872D7A2C60A6
GO
Isoform 2 (identifier: Q9Y2I2-2) [UniParc]FASTAAdd to basket
Also known as: 1F

The sequence of this isoform differs from the canonical sequence as follows:
     363-364: NC → SK
     365-539: Missing.

Show »
Length:364
Mass (Da):41,759
Checksum:iB23DAF4BEDE9271C
GO
Isoform 1 (identifier: Q9Y2I2-1) [UniParc]FASTAAdd to basket
Also known as: 1C

The sequence of this isoform differs from the canonical sequence as follows:
     363-463: Missing.
     464-464: P → T

Note: Hi expression in Expressed in brain and.
Show »
Length:438
Mass (Da):49,340
Checksum:iBBBA6167FAC8DD71
GO
Isoform 4 (identifier: Q9Y2I2-4) [UniParc]FASTAAdd to basket
Also known as: 1D

The sequence of this isoform differs from the canonical sequence as follows:
     364-464: CECFGHSNRC...GNSWHYGCQP → PPKFNRIWPN...SVQVANHKRA

Note: Mostly expressed in kidney, also expressed in adult and fetal brain.
Show »
Length:480
Mass (Da):53,946
Checksum:i9099C3CF4D3F7B86
GO
Isoform 5 (identifier: Q9Y2I2-5) [UniParc]FASTAAdd to basket
Also known as: 1E

The sequence of this isoform differs from the canonical sequence as follows:
     364-385: CECFGHSNRCSYIDLLNTVICV → PPKFNRIWPNISSLEVSNPKQA
     386-464: Missing.

Note: Some expression in fetal brain.
Show »
Length:460
Mass (Da):51,858
Checksum:i8217255F4C8C4C65
GO
Isoform 6 (identifier: Q9Y2I2-6) [UniParc]FASTAAdd to basket
Also known as: 1G

The sequence of this isoform differs from the canonical sequence as follows:
     419-463: Missing.
     464-464: P → A

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):55,660
Checksum:iF6783893CC0FE0D8
GO

Sequence cautioni

The sequence BAA76820 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6F → S in AAQ88731 (PubMed:12975309).Curated1
Sequence conflicti231F → L in AAQ88731 (PubMed:12975309).Curated1
Sequence conflicti521S → T in AAQ88731 (PubMed:12975309).Curated1
Isoform 2 (identifier: Q9Y2I2-2)
Sequence conflicti364K → KQ in CAD98143 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012574363 – 463Missing in isoform 1. 2 PublicationsAdd BLAST101
Alternative sequenceiVSP_010429363 – 364NC → SK in isoform 2. 2 Publications2
Alternative sequenceiVSP_012575364 – 464CECFG…YGCQP → PPKFNRIWPNISSLEVSNPK QVAPKLALSTVSSVQVANHK RA in isoform 4. CuratedAdd BLAST101
Alternative sequenceiVSP_012576364 – 385CECFG…TVICV → PPKFNRIWPNISSLEVSNPK QA in isoform 5. CuratedAdd BLAST22
Alternative sequenceiVSP_010430365 – 539Missing in isoform 2. 2 PublicationsAdd BLAST175
Alternative sequenceiVSP_012577386 – 464Missing in isoform 5. CuratedAdd BLAST79
Alternative sequenceiVSP_012578419 – 463Missing in isoform 6. CuratedAdd BLAST45
Alternative sequenceiVSP_012579464P → T in isoform 1. 2 Publications1
Alternative sequenceiVSP_012580464P → A in isoform 6. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023193 mRNA Translation: BAA76820.2 Different initiation.
AY358365 mRNA Translation: AAQ88731.1
BX538348 mRNA Translation: CAD98143.1
AC114491 Genomic DNA No translation available.
AL513187 Genomic DNA No translation available.
AL590427 Genomic DNA No translation available.
BC030220 mRNA Translation: AAH30220.1
CCDSiCCDS30785.1 [Q9Y2I2-1]
CCDS44179.1 [Q9Y2I2-4]
CCDS44180.1 [Q9Y2I2-3]
CCDS81354.1 [Q9Y2I2-5]
RefSeqiNP_001106697.1, NM_001113226.2 [Q9Y2I2-3]
NP_001106699.1, NM_001113228.2 [Q9Y2I2-4]
NP_001317594.1, NM_001330665.1 [Q9Y2I2-5]
NP_055732.2, NM_014917.3 [Q9Y2I2-1]
XP_006710519.1, XM_006710456.3 [Q9Y2I2-1]
XP_011539323.1, XM_011541021.1 [Q9Y2I2-3]
XP_016856169.1, XM_017000680.1 [Q9Y2I2-3]
XP_016856170.1, XM_017000681.1 [Q9Y2I2-3]
XP_016856174.1, XM_017000685.1 [Q9Y2I2-1]
XP_016856175.1, XM_017000686.1 [Q9Y2I2-2]
UniGeneiHs.133046
Hs.732535

Genome annotation databases

EnsembliENST00000370065; ENSP00000359082; ENSG00000162631 [Q9Y2I2-6]
ENST00000370066; ENSP00000359083; ENSG00000162631 [Q9Y2I2-4]
ENST00000370067; ENSP00000359084; ENSG00000162631 [Q9Y2I2-5]
ENST00000370068; ENSP00000359085; ENSG00000162631 [Q9Y2I2-3]
ENST00000370071; ENSP00000359088; ENSG00000162631 [Q9Y2I2-4]
ENST00000370073; ENSP00000359090; ENSG00000162631 [Q9Y2I2-3]
ENST00000370074; ENSP00000359091; ENSG00000162631 [Q9Y2I2-1]
GeneIDi22854
KEGGihsa:22854
UCSCiuc001dvc.4 human [Q9Y2I2-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNTNG1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2I2
Secondary accession number(s): Q5VU86
, Q5VU87, Q5VU89, Q5VU90, Q5VU91, Q7Z2Y3, Q8N633
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: January 4, 2005
Last modified: May 23, 2018
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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