Q9Y2I1 (NISCH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nischarin

Alternative name(s):
Imidazoline receptor 1
Short name=I-1
Short name=IR1
Imidazoline receptor antisera-selected protein
Short name=hIRAS
Imidazoline-1 receptor
Short name=I1R
Imidazoline-1 receptor candidate protein
Short name=I-1 receptor candidate protein
Short name=I1R candidate protein

Gene names

Name:	NISCH
Synonyms:	IRAS, KIAA0975

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension By similarity. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons By similarity. Acts as a modulator of Rac-regulated signal transduction pathways By similarity. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity By similarity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation By similarity. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation By similarity. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells By similarity. Inhibits lamellipodia formation, when overexpressed By similarity. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures. Ref.1 Ref.2 Ref.11 Ref.12 Ref.13
Subunit structure	Homooligomer. Interacts with GRB2. Interacts with PIK3R1; probably associates with the PI3-kinase complex. Interacts with IRS4. Found in a complex with ITGA5 and PAK1. Found in a complex with LIMK1 and PAK1. Interacts with ITGA5 (via cytoplasmic domain); this interaction is direct. Interacts with PAK1 (via kinase domain); this interaction is direct and is increased upon activation of PAK1 By similarity. Interacts with LIMK1 (via PDZ and kinase domain); this interaction is direct By similarity. Interacts with LIMK2; this interaction depends on LIMK2 activity By similarity. Interacts with RAC1 (activated state) By similarity. Ref.10 Ref.13
Subcellular location	Cell membrane. Cytoplasm. Early endosome. Recycling endosome. Note: Enriched in the early/sorting and recycling endosomes. Colocalized in early/sorting endosomes with EEA1 and SNX2 and in recycling endosomes with transferrin receptor. Detected in the perinuclear region partially associated with punctate structures By similarity. Colocalizes with PAK1 in cytoplasm, vesicular structures in the perinuclear area and membrane ruffles By similarity. Colocalizes with RAC1 in the cytoplasm and vesicles structures By similarity. Colocalized with MAPK1 and MAPK3 in RVLM neurons By similarity. Ref.1 Ref.10 Ref.12 Ref.13
Tissue specificity	Isoform 1, isoform 3 and isoform 4 are expressed in brain. Isoform 1 is expressed in endocrine tissues. Ref.2 Ref.9
Domain	Both the presence of the PX domain and the coiled coil region are necessary for its endosomal targeting.
Sequence similarities	Contains 6 LRR (leucine-rich) repeats. Contains 1 PX (phox homology) domain.
Sequence caution	The sequence BAA76819.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell membrane Cytoplasm Endosome Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Leucine-rich repeat Repeat
Molecular function	Receptor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rac protein signal transduction Inferred from electronic annotation. Source: Compara actin cytoskeleton organization Inferred from electronic annotation. Source: Compara apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW glucose metabolic process Inferred from electronic annotation. Source: Compara negative regulation of cell migration Inferred from electronic annotation. Source: Compara norepinephrine secretion Inferred from electronic annotation. Source: Compara regulation of blood pressure Inferred from electronic annotation. Source: Compara regulation of synaptic transmission, GABAergic Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from direct assay PubMed 15028622. Source: BHF-UCL early endosome Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 15028622. Source: BHF-UCL recycling endosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled amine receptor activity Inferred from electronic annotation. Source: Compara phosphatidylinositol binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2I1-1)

Also known as: IRAS-1; IRAS-M;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2I1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-511: Missing.

Isoform 3 (identifier: Q9Y2I1-3)

Also known as: IRAS-L;

The sequence of this isoform differs from the canonical sequence as follows:
     511-583: IMFVQEEALA...PEVQVVPGSG → NRVCTLLLVE...CLLGEDSQLL
     584-1504: Missing.

Isoform 4 (identifier: Q9Y2I1-4)

Also known as: IRAS-S;

The sequence of this isoform differs from the canonical sequence as follows:
     512-515: MFVQ → LGDE
     516-1504: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1504

1504

Nischarin

PRO_0000348265

Regions

Domain

11 – 121

111

PX

Repeat

288 – 309

22

LRR 1

Repeat

311 – 332

22

LRR 2

Repeat

333 – 354

22

LRR 3

Repeat

356 – 377

22

LRR 4

Repeat

378 – 399

22

LRR 5

Repeat

403 – 424

22

LRR 6

Region

1 – 133

133

Necessary for binding to phosphoinositide-3-P; not sufficient for targeting to endosomes

Region

120 – 695

576

Necessary for homooligomerization and targeting to endosomes

Region

245 – 869

625

Interaction with PAK1 By similarity

Region

660 – 869

210

Interaction with LIMK By similarity

Region

709 – 807

99

Interaction with ITGA5 By similarity

Coiled coil

634 – 695

62

Potential

Compositional bias

629 – 691

63

Glu-rich

Compositional bias

1048 – 1104

57

Ala/Pro-rich

Amino acid modifications

Modified residue

1284

1

Phosphoserine Ref.14

Natural variations

Alternative sequence

1 – 511

511

Missing in isoform 2.

VSP_035131

Alternative sequence

511 – 583

73

IMFVQ…VPGSG → NRVCTLLLVEPHSPAWAPWL GWGWGRGASTCFQQGTQGGG QCLLQAGPRGGTHGRGAWPD ASCCLLGEDSQLL in isoform 3.

VSP_035132

Alternative sequence

512 – 515

4

MFVQ → LGDE in isoform 4.

VSP_035133

Alternative sequence

516 – 1504

989

Missing in isoform 4.

VSP_035134

Alternative sequence

584 – 1504

921

Missing in isoform 3.

VSP_035135

Natural variant

299

1

V → I. Ref.1 Ref.2 Ref.3 Ref.4 Ref.7 Ref.8
Corresponds to variant rs9856575 [ dbSNP | Ensembl ].

VAR_046130

Natural variant

1056

1

A → V. Ref.1 Ref.3 Ref.4 Ref.5 Ref.7 Ref.8 Ref.9
Corresponds to variant rs887515 [ dbSNP | Ensembl ].

VAR_046131

Experimental info

Mutagenesis

49

1

R → A: Inhibits targeting to endosomes. Ref.13

Mutagenesis

50

1

Y → A: Inhibits targeting to endosomes. Ref.13

Sequence conflict

927

1

M → I in AAH56900. Ref.8

Sequence conflict

1023

1

P → A in BAA76819. Ref.3

Sequence conflict

1123

1

S → P in AAH38102. Ref.8

Sequence conflict

1382

1

L → F in AAH54494. Ref.8

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1504

Helix Strand Turn

Details...

Beta strand

18 – 25

8

Beta strand

27 – 30

4

Beta strand

32 – 38

7

Beta strand

43 – 49

7

Helix

50 – 62

13

Helix

85 – 101

17

Beta strand

105 – 107

3

Helix

109 – 116

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (IRAS-1) (IRAS-M) [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 7761C7DA1FDC4E53	FASTA	1,504	166,629
	10 20 30 40 50 60 MATARTFGPE REAEPAKEAR VVGSELVDTY TVYIIQVTDG SHEWTVKHRY SDFHDLHEKL 70 80 90 100 110 120 VAERKIDKNL LPPKKIIGKN SRSLVEKREK DLEVYLQKLL AAFPGVTPRV LAHFLHFHFY 130 140 150 160 170 180 EINGITAALA EELFEKGEQL LGAGEVFAIG PLQLYAVTEQ LQQGKPTCAS GDAKTDLGHI 190 200 210 220 230 240 LDFTCRLKYL KVSGTEGPFG TSNIQEQLLP FDLSIFKSLH QVEISHCDAK HIRGLVASKP 250 260 270 280 290 300 TLATLSVRFS ATSMKEVLVP EASEFDEWEP EGTTLEGPVT AVIPTWQALT TLDLSHNSVS 310 320 330 340 350 360 EIDESVKLIP KIEFLDLSHN GLLVVDNLQH LYNLVHLDLS YNKLSSLEGL HTKLGNIKTL 370 380 390 400 410 420 NLAGNLLESL SGLHKLYSLV NLDLRDNRIE QMEEVRSIGS LPCLEHVSLL NNPLSIIPDY 430 440 450 460 470 480 RTKVLAQFGE RASEVCLDDT VTTEKELDTV EVLKAIQKAK EVKSKLSNPE KKGGEDSRLS 490 500 510 520 530 540 AAPCIRPSSS PPTVAPASAS LPQPILSNQG IMFVQEEALA SSLSSTDSLT PEHQPIAQGC 550 560 570 580 590 600 SDSLESIPAG QAASDDLRDV PGAVGGASPE HAEPEVQVVP GSGQIIFLPF TCIGYTATNQ 610 620 630 640 650 660 DFIQRLSTLI RQAIERQLPA WIEAANQREE GQGEQGEEED EEEEEEEDVA ENRYFEMGPP 670 680 690 700 710 720 DVEEEEGGGQ GEEEEEEEED EEAEEERLAL EWALGADEDF LLEHIRILKV LWCFLIHVQG 730 740 750 760 770 780 SIRQFAACLV LTDFGIAVFE IPHQESRGSS QHILSSLRFV FCFPHGDLTE FGFLMPELCL 790 800 810 820 830 840 VLKVRHSENT LFIISDAANL HEFHADLRSC FAPQHMAMLC SPILYGSHTS LQEFLRQLLT 850 860 870 880 890 900 FYKVAGGCQE RSQGCFPVYL VYSDKRMVQT AAGDYSGNIE WASCTLCSAV RRSCCAPSEA 910 920 930 940 950 960 VKSAAIPYWL LLTPQHLNVI KADFNPMPNR GTHNCRNRNS FKLSRVPLST VLLDPTRSCT 970 980 990 1000 1010 1020 QPRGAFADGH VLELLVGYRF VTAIFVLPHE KFHFLRVYNQ LRASLQDLKT VVIAKTPGTG 1030 1040 1050 1060 1070 1080 GSPQGSFADG QPAERRASND QRPQEVPAEA LAPAPAEVPA PAPAAASASG PAKTPAPAEA 1090 1100 1110 1120 1130 1140 STSALVPEET PVEAPAPPPA EAPAQYPSEH LIQATSEENQ IPSHLPACPS LRHVASLRGS 1150 1160 1170 1180 1190 1200 AIIELFHSSI AEVENEELRH LMWSSVVFYQ TPGLEVTACV LLSTKAVYFV LHDGLRRYFS 1210 1220 1230 1240 1250 1260 EPLQDFWHQK NTDYNNSPFH ISQCFVLKLS DLQSVNVGLF DQHFRLTGST PMQVVTCLTR 1270 1280 1290 1300 1310 1320 DSYLTHCFLQ HLMVVLSSLE RTPSPEPVDK DFYSEFGNKT TGKMENYELI HSSRVKFTYP 1330 1340 1350 1360 1370 1380 SEEEIGDLTF TVAQKMAEPE KAPALSILLY VQAFQVGMPP PGCCRGPLRP KTLLLTSSEI 1390 1400 1410 1420 1430 1440 FLLDEDCVHY PLPEFAKEPP QRDRYRLDDG RRVRDLDRVL MGYQTYPQAL TLVFDDVQGH 1450 1460 1470 1480 1490 1500 DLMGSVTLDH FGEVPGGPAR ASQGREVQWQ VFVPSAESRE KLISLLARQW EALCGRELPV ELTG
	Isoform 2 [UniParc]. Checksum: E0415818937AA523	FASTA	993	110,194
	10 20 30 40 50 60 MFVQEEALAS SLSSTDSLTP EHQPIAQGCS DSLESIPAGQ AASDDLRDVP GAVGGASPEH 70 80 90 100 110 120 AEPEVQVVPG SGQIIFLPFT CIGYTATNQD FIQRLSTLIR QAIERQLPAW IEAANQREEG 130 140 150 160 170 180 QGEQGEEEDE EEEEEEDVAE NRYFEMGPPD VEEEEGGGQG EEEEEEEEDE EAEEERLALE 190 200 210 220 230 240 WALGADEDFL LEHIRILKVL WCFLIHVQGS IRQFAACLVL TDFGIAVFEI PHQESRGSSQ 250 260 270 280 290 300 HILSSLRFVF CFPHGDLTEF GFLMPELCLV LKVRHSENTL FIISDAANLH EFHADLRSCF 310 320 330 340 350 360 APQHMAMLCS PILYGSHTSL QEFLRQLLTF YKVAGGCQER SQGCFPVYLV YSDKRMVQTA 370 380 390 400 410 420 AGDYSGNIEW ASCTLCSAVR RSCCAPSEAV KSAAIPYWLL LTPQHLNVIK ADFNPMPNRG 430 440 450 460 470 480 THNCRNRNSF KLSRVPLSTV LLDPTRSCTQ PRGAFADGHV LELLVGYRFV TAIFVLPHEK 490 500 510 520 530 540 FHFLRVYNQL RASLQDLKTV VIAKTPGTGG SPQGSFADGQ PAERRASNDQ RPQEVPAEAL 550 560 570 580 590 600 APAPAEVPAP APAAASASGP AKTPAPAEAS TSALVPEETP VEAPAPPPAE APAQYPSEHL 610 620 630 640 650 660 IQATSEENQI PSHLPACPSL RHVASLRGSA IIELFHSSIA EVENEELRHL MWSSVVFYQT 670 680 690 700 710 720 PGLEVTACVL LSTKAVYFVL HDGLRRYFSE PLQDFWHQKN TDYNNSPFHI SQCFVLKLSD 730 740 750 760 770 780 LQSVNVGLFD QHFRLTGSTP MQVVTCLTRD SYLTHCFLQH LMVVLSSLER TPSPEPVDKD 790 800 810 820 830 840 FYSEFGNKTT GKMENYELIH SSRVKFTYPS EEEIGDLTFT VAQKMAEPEK APALSILLYV 850 860 870 880 890 900 QAFQVGMPPP GCCRGPLRPK TLLLTSSEIF LLDEDCVHYP LPEFAKEPPQ RDRYRLDDGR 910 920 930 940 950 960 RVRDLDRVLM GYQTYPQALT LVFDDVQGHD LMGSVTLDHF GEVPGGPARA SQGREVQWQV 970 980 990 FVPSAESREK LISLLARQWE ALCGRELPVE LTG
	Isoform 3 (IRAS-L) [UniParc]. Checksum: 83CED19440961191	FASTA	583	63,997
	10 20 30 40 50 60 MATARTFGPE REAEPAKEAR VVGSELVDTY TVYIIQVTDG SHEWTVKHRY SDFHDLHEKL 70 80 90 100 110 120 VAERKIDKNL LPPKKIIGKN SRSLVEKREK DLEVYLQKLL AAFPGVTPRV LAHFLHFHFY 130 140 150 160 170 180 EINGITAALA EELFEKGEQL LGAGEVFAIG PLQLYAVTEQ LQQGKPTCAS GDAKTDLGHI 190 200 210 220 230 240 LDFTCRLKYL KVSGTEGPFG TSNIQEQLLP FDLSIFKSLH QVEISHCDAK HIRGLVASKP 250 260 270 280 290 300 TLATLSVRFS ATSMKEVLVP EASEFDEWEP EGTTLEGPVT AVIPTWQALT TLDLSHNSVS 310 320 330 340 350 360 EIDESVKLIP KIEFLDLSHN GLLVVDNLQH LYNLVHLDLS YNKLSSLEGL HTKLGNIKTL 370 380 390 400 410 420 NLAGNLLESL SGLHKLYSLV NLDLRDNRIE QMEEVRSIGS LPCLEHVSLL NNPLSIIPDY 430 440 450 460 470 480 RTKVLAQFGE RASEVCLDDT VTTEKELDTV EVLKAIQKAK EVKSKLSNPE KKGGEDSRLS 490 500 510 520 530 540 AAPCIRPSSS PPTVAPASAS LPQPILSNQG NRVCTLLLVE PHSPAWAPWL GWGWGRGAST 550 560 570 580 CFQQGTQGGG QCLLQAGPRG GTHGRGAWPD ASCCLLGEDS QLL
	Isoform 4 (IRAS-S) [UniParc]. Checksum: B623BE4D42BBA51C	FASTA	515	56,867
	10 20 30 40 50 60 MATARTFGPE REAEPAKEAR VVGSELVDTY TVYIIQVTDG SHEWTVKHRY SDFHDLHEKL 70 80 90 100 110 120 VAERKIDKNL LPPKKIIGKN SRSLVEKREK DLEVYLQKLL AAFPGVTPRV LAHFLHFHFY 130 140 150 160 170 180 EINGITAALA EELFEKGEQL LGAGEVFAIG PLQLYAVTEQ LQQGKPTCAS GDAKTDLGHI 190 200 210 220 230 240 LDFTCRLKYL KVSGTEGPFG TSNIQEQLLP FDLSIFKSLH QVEISHCDAK HIRGLVASKP 250 260 270 280 290 300 TLATLSVRFS ATSMKEVLVP EASEFDEWEP EGTTLEGPVT AVIPTWQALT TLDLSHNSVS 310 320 330 340 350 360 EIDESVKLIP KIEFLDLSHN GLLVVDNLQH LYNLVHLDLS YNKLSSLEGL HTKLGNIKTL 370 380 390 400 410 420 NLAGNLLESL SGLHKLYSLV NLDLRDNRIE QMEEVRSIGS LPCLEHVSLL NNPLSIIPDY 430 440 450 460 470 480 RTKVLAQFGE RASEVCLDDT VTTEKELDTV EVLKAIQKAK EVKSKLSNPE KKGGEDSRLS 490 500 510 AAPCIRPSSS PPTVAPASAS LPQPILSNQG ILGDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Imidazoline receptor antisera-selected (IRAS) cDNA: cloning and characterization." Piletz J.E., Ivanov T.R., Sharp J.D., Ernsberger P., Chang C.-H., Pickard R.T., Gold G., Roth B., Zhu H., Jones J.C., Baldwin J., Reis D.J. DNA Cell Biol. 19:319-329(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, VARIANTS ILE-299 AND VAL-1056.
[2]	"IRAS splice variants." Piletz J.E., Deleersnijder W., Roth B.L., Ernsberger P., Zhu H., Ziegler D. Ann. N. Y. Acad. Sci. 1009:419-426(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3 AND 4), FUNCTION, TISSUE SPECIFICITY, VARIANT ILE-299.
[3]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ILE-299 AND VAL-1056. Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ILE-299 AND VAL-1056. Tissue: Brain.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT VAL-1056. Tissue: Testis.
[6]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS ILE-299 AND VAL-1056.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ILE-299 AND VAL-1056. Tissue: Eye, PNS and Testis.
[9]	"Characterization of a partial cDNA clone detected by imidazoline receptor-selective antisera." Ivanov T.R., Jones J.C., Dontenwill M., Bousquet P., Piletz J.E. J. Auton. Nerv. Syst. 72:98-110(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 469-1063 (ISOFORM 1), TISSUE SPECIFICITY, VARIANT VAL-1056. Tissue: Hippocampus.
[10]	"Insulin receptor substrate 4 associates with the protein IRAS." Sano H., Liu S.C.H., Lane W.S., Piletz J.E., Lienhard G.E. J. Biol. Chem. 277:19439-19447(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GRB2; IRS4 AND PIK3R1, SUBCELLULAR LOCATION.
[11]	"IRAS is an anti-apoptotic protein." Dontenwill M., Piletz J.E., Chen M., Baldwin J., Pascal G., Ronde P., Dupuy L., Greney H., Takeda K., Bousquetd P. Ann. N. Y. Acad. Sci. 1009:400-412(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"IRAS, the human homologue of Nischarin, prolongs survival of transfected PC12 cells." Dontenwill M., Pascal G., Piletz J.E., Chen M., Baldwin J., Ronde P., Dupuy L., Urosevic D., Greney H., Takeda K., Bousquet P. Cell Death Differ. 10:933-935(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]	"Human Nischarin/imidazoline receptor antisera-selected protein is targeted to the endosomes by a combined action of a PX domain and a coiled-coil region." Lim K.-P., Hong W. J. Biol. Chem. 279:54770-54782(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH ITGA5, MUTAGENESIS OF ARG-49 AND TYR-50, SUBCELLULAR LOCATION.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1284, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"Crystal structure of nischarin PX-domain." Structural genomics consortium (SGC) Submitted (OCT-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.27 ANGSTROMS) OF 18-124.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF082516 mRNA. Translation: AAC33104.1.
AB023192 mRNA. Translation: BAA76819.1. Different initiation.
AK291398 mRNA. Translation: BAF84087.1.
AL117432 mRNA. Translation: CAB55920.1.
AC006208 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW65229.1.
BC038102 mRNA. Translation: AAH38102.1.
BC054494 mRNA. Translation: AAH54494.1.
BC056900 mRNA. Translation: AAH56900.1.
AF058290 mRNA. Translation: AAC33321.1.

IPI

IPI00032187.
IPI00792206.
IPI00795420.
IPI00902963.

PIR

T17230.

RefSeq

NP_009115.2. NM_007184.3.

UniGene

Hs.435290.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P0C	X-ray	2.27	A/B	18-124	[»]

ProteinModelPortal

Q9Y2I1.

SMR

Q9Y2I1. Positions 18-123, 289-468.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y2I1. 4 interactions.

STRING

9606.ENSP00000339958.

PTM databases

PhosphoSite

Q9Y2I1.

Polymorphism databases

DMDM

296439287.

Proteomic databases

PaxDb

Q9Y2I1.

PRIDE

Q9Y2I1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000345716; ENSP00000339958; ENSG00000010322.
ENST00000420808; ENSP00000392484; ENSG00000010322.
ENST00000479054; ENSP00000418232; ENSG00000010322.

GeneID

11188.

KEGG

hsa:11188.

UCSC

uc003ded.4. human.

Organism-specific databases

CTD

11188.

GeneCards

GC03P052489.

HGNC

HGNC:18006. NISCH.

HPA

HPA023189.

neXtProt

NX_Q9Y2I1.

PharmGKB

PA31635.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG269110.

HOVERGEN

HBG108189.

InParanoid

Q9Y2I1.

OMA

TEFGFLM.

OrthoDB

EOG4N5VW2.

Gene expression databases

ArrayExpress

Q9Y2I1.

Bgee

Q9Y2I1.

CleanEx

HS_NISCH.

Genevestigator

Q9Y2I1.

Family and domain databases

Gene3D

3.30.1520.10. 1 hit.

InterPro

IPR001611. Leu-rich_rpt.
IPR001683. Phox.
[Graphical view]

Pfam

PF00787. PX. 1 hit.
[Graphical view]

SMART

SM00312. PX. 1 hit.
[Graphical view]

SUPFAM

SSF64268. PX. 1 hit.

PROSITE

PS51450. LRR. 6 hits.
PS50195. PX. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL3923.

ChiTaRS

NISCH. human.

GenomeRNAi

11188.

NextBio

42587.

Entry information

Entry name

NISCH_HUMAN

Accession

Primary (citable) accession number: Q9Y2I1
Secondary accession number(s): C9J245 , Q6PGP3, Q6PIB4, Q7L8M3, Q7Z2X6, Q9UES6, Q9UEU4, Q9UFW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.