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Q9Y2H9

- MAST1_HUMAN

UniProt

Q9Y2H9 - MAST1_HUMAN

Protein

Microtubule-associated serine/threonine-protein kinase 1

Gene

MAST1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei403 – 4031ATPBy similarityPROSITE-ProRule annotation
    Active sitei497 – 4971Proton acceptorBy similarityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi380 – 3889ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cytoskeleton organization Source: UniProtKB
    2. intracellular signal transduction Source: UniProtKB
    3. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ9Y2H9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated serine/threonine-protein kinase 1 (EC:2.7.11.1)
    Alternative name(s):
    Syntrophin-associated serine/threonine-protein kinase
    Gene namesi
    Name:MAST1Imported
    Synonyms:KIAA0973Imported, SAST
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:19034. MAST1.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmcytoskeleton By similarity
    Note: Colocalizes with syntrophins at the cell membrane.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. cytoskeleton Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134946727.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15701570Microtubule-associated serine/threonine-protein kinase 1PRO_0000086309Add
    BLAST

    Proteomic databases

    MaxQBiQ9Y2H9.
    PaxDbiQ9Y2H9.
    PeptideAtlasiQ9Y2H9.
    PRIDEiQ9Y2H9.

    PTM databases

    PhosphoSiteiQ9Y2H9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Y2H9.
    BgeeiQ9Y2H9.
    CleanExiHS_MAST1.
    GenevestigatoriQ9Y2H9.

    Organism-specific databases

    HPAiHPA028181.

    Interactioni

    Subunit structurei

    Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EGFRP005332EBI-3385920,EBI-297353

    Protein-protein interaction databases

    BioGridi116632. 36 interactions.
    IntActiQ9Y2H9. 32 interactions.
    STRINGi9606.ENSP00000251472.

    Structurei

    Secondary structure

    1
    1570
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi189 – 20214
    Helixi216 – 23722
    Helixi242 – 26120
    Helixi268 – 28114
    Beta strandi968 – 9714
    Beta strandi973 – 9753
    Beta strandi978 – 98710
    Beta strandi994 – 100310
    Helixi1008 – 10125
    Beta strandi1019 – 10235
    Helixi1033 – 104210
    Turni1043 – 10453
    Beta strandi1046 – 10527

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2M9XNMR-A187-287[»]
    3PS4X-ray1.85A/B/C/D965-1057[»]
    ProteinModelPortaliQ9Y2H9.
    SMRiQ9Y2H9. Positions 187-713, 965-1057.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9Y2H9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini374 – 647274Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini648 – 71972AGC-kinase C-terminalAdd
    BLAST
    Domaini967 – 105589PDZPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000046662.
    HOVERGENiHBG052414.
    InParanoidiQ9Y2H9.
    KOiK08789.
    OMAiGCTRHQS.
    OrthoDBiEOG7PK8ZC.
    PhylomeDBiQ9Y2H9.
    TreeFamiTF313149.

    Family and domain databases

    Gene3Di1.20.1480.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR015022. MA_Ser/Thr_Kinase_dom.
    IPR028777. MAST1.
    IPR023142. MAST_pre-PK_dom.
    IPR001478. PDZ.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24356:SF150. PTHR24356:SF150. 1 hit.
    PfamiPF08926. DUF1908. 1 hit.
    PF00595. PDZ. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF140482. SSF140482. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Y2H9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH     50
    SPLPGHLGSS PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG 100
    TNTPSSTVSS SCSSQERLHQ LPYQPTVDEL HFLSKHFGST ESITDEDGGR 150
    RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH VYKERFPKAT AQMEEKLRDF 200
    TRAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI TTVYFYELQE 250
    NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY 300
    HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVH LEEQDSGGSN 350
    TPEQDDLSEG RSSKAKKPPG ENDFDTIKLI SNGAYGAVYL VRHRDTRQRF 400
    AMKKINKQNL ILRNQIQQAF VERDILTFAE NPFVVGMFCS FETRRHLCMV 450
    MEYVEGGDCA TLLKNIGALP VEMARMYFAE TVLALEYLHN YGIVHRDLKP 500
    DNLLITSMGH IKLTDFGLSK MGLMSLTTNL YEGHIEKDAR EFLDKQVCGT 550
    PEYIAPEVIL RQGYGKPVDW WAMGIILYEF LVGCVPFFGD TPEELFGQVI 600
    SDDILWPEGD EALPTEAQLL ISSLLQTNPL VRLGAGGAFE VKQHSFFRDL 650
    DWTGLLRQKA EFIPHLESED DTSYFDTRSD RYHHVNSYDE DDTTEEEPVE 700
    IRQFSSCSPR FSKVYSSMEQ LSQHEPKTPV AAAGSSKREP STKGPEEKVA 750
    GKREGLGGLT LREKTWRGGS PEIKRFSASE ASFLEGEASP PLGARRRFSA 800
    LLEPSRFSAP QEDEDEARLR RPPRPSSDPA GSLDARAPKE ETQGEGTSSA 850
    GDSEATDRPR PGDLCPPSKD GDASGPRATN DLVLRRARHQ QMSGDVAVEK 900
    RPSRTGGKVI KSASATALSV MIPAVDPHGS SPLASPMSPR SLSSNPSSRD 950
    SSPSRDYSPA VSGLRSPITI QRSGKKYGFT LRAIRVYMGD TDVYSVHHIV 1000
    WHVEEGGPAQ EAGLCAGDLI THVNGEPVHG MVHPEVVELI LKSGNKVAVT 1050
    TTPFENTSIR IGPARRSSYK AKMARRNKRP SAKEGQESKK RSSLFRKITK 1100
    QSNLLHTSRS LSSLNRSLSS SDSLPGSPTH GLPARSPTHS YRSTPDSAYL 1150
    GASSQSSSPA SSTPNSPASS ASHHIRPSTL HGLSPKLHRQ YRSARCKSAG 1200
    NIPLSPLAHT PSPTQASPPP LPGHTVGSSH TTQSFPAKLH SSPPVVRPRP 1250
    KSAEPPRSPL LKRVQSAEKL GASLSADKKG ALRKHSLEVG HPDFRKDFHG 1300
    ELALHSLAES DGETPPVEGL GAPRQVAVRR LGRQESPLSL GADPLLPEGA 1350
    SRPPVSSKEK ESPGGAEACT PPRATTPGGR TLERDVGCTR HQSVQTEDGT 1400
    GGMARAVAKA ALSPVQEHET GRRSSSGEAG TPLVPIVVEP ARPGAKAVVP 1450
    QPLGADSKGL QEPAPLAPSV PEAPRGRERW VLEVVEERTT LSGPRSKPAS 1500
    PKLSPEPQTP SLAPAKCSAP SSAVTPVPPA SLLGSGTKPQ VGLTSRCPAE 1550
    AVPPAGLTKK GVSSPAPPGP 1570
    Length:1,570
    Mass (Da):170,677
    Last modified:March 1, 2005 - v2
    Checksum:i189D24AE07B62A1E
    GO

    Sequence cautioni

    The sequence AAH27985.2 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA76817.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAB51171.1 differs from that shown. Reason: Erroneous gene model prediction.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti269 – 2691A → T in a metastatic melanoma sample; somatic mutation. 1 Publication
    VAR_040768
    Natural varianti1048 – 10481A → S.
    Corresponds to variant rs35052801 [ dbSNP | Ensembl ].
    VAR_051644
    Natural varianti1240 – 12401H → Y in an ovarian serous carcinoma sample; somatic mutation. 1 Publication
    VAR_040769
    Natural varianti1292 – 12921P → S.1 Publication
    Corresponds to variant rs35071862 [ dbSNP | Ensembl ].
    VAR_040770

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023190 mRNA. Translation: BAA76817.1. Different initiation.
    AC020934 Genomic DNA. No translation available.
    AD000092 Genomic DNA. Translation: AAB51171.1. Sequence problems.
    BC027985 mRNA. Translation: AAH27985.2. Different initiation.
    CCDSiCCDS32921.1.
    PIRiT45070.
    RefSeqiNP_055790.1. NM_014975.2.
    UniGeneiHs.227489.

    Genome annotation databases

    EnsembliENST00000251472; ENSP00000251472; ENSG00000105613.
    GeneIDi22983.
    KEGGihsa:22983.
    UCSCiuc002mvm.3. human.

    Polymorphism databases

    DMDMi60390226.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023190 mRNA. Translation: BAA76817.1 . Different initiation.
    AC020934 Genomic DNA. No translation available.
    AD000092 Genomic DNA. Translation: AAB51171.1 . Sequence problems.
    BC027985 mRNA. Translation: AAH27985.2 . Different initiation.
    CCDSi CCDS32921.1.
    PIRi T45070.
    RefSeqi NP_055790.1. NM_014975.2.
    UniGenei Hs.227489.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2M9X NMR - A 187-287 [» ]
    3PS4 X-ray 1.85 A/B/C/D 965-1057 [» ]
    ProteinModelPortali Q9Y2H9.
    SMRi Q9Y2H9. Positions 187-713, 965-1057.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116632. 36 interactions.
    IntActi Q9Y2H9. 32 interactions.
    STRINGi 9606.ENSP00000251472.

    Chemistry

    BindingDBi Q9Y2H9.
    ChEMBLi CHEMBL3038500.
    GuidetoPHARMACOLOGYi 1510.

    PTM databases

    PhosphoSitei Q9Y2H9.

    Polymorphism databases

    DMDMi 60390226.

    Proteomic databases

    MaxQBi Q9Y2H9.
    PaxDbi Q9Y2H9.
    PeptideAtlasi Q9Y2H9.
    PRIDEi Q9Y2H9.

    Protocols and materials databases

    DNASUi 22983.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000251472 ; ENSP00000251472 ; ENSG00000105613 .
    GeneIDi 22983.
    KEGGi hsa:22983.
    UCSCi uc002mvm.3. human.

    Organism-specific databases

    CTDi 22983.
    GeneCardsi GC19P012949.
    HGNCi HGNC:19034. MAST1.
    HPAi HPA028181.
    MIMi 612256. gene.
    neXtProti NX_Q9Y2H9.
    PharmGKBi PA134946727.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000046662.
    HOVERGENi HBG052414.
    InParanoidi Q9Y2H9.
    KOi K08789.
    OMAi GCTRHQS.
    OrthoDBi EOG7PK8ZC.
    PhylomeDBi Q9Y2H9.
    TreeFami TF313149.

    Enzyme and pathway databases

    SignaLinki Q9Y2H9.

    Miscellaneous databases

    ChiTaRSi MAST1. human.
    EvolutionaryTracei Q9Y2H9.
    GeneWikii MAST1.
    GenomeRNAii 22983.
    NextBioi 43815.
    PROi Q9Y2H9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y2H9.
    Bgeei Q9Y2H9.
    CleanExi HS_MAST1.
    Genevestigatori Q9Y2H9.

    Family and domain databases

    Gene3Di 1.20.1480.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR015022. MA_Ser/Thr_Kinase_dom.
    IPR028777. MAST1.
    IPR023142. MAST_pre-PK_dom.
    IPR001478. PDZ.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24356:SF150. PTHR24356:SF150. 1 hit.
    Pfami PF08926. DUF1908. 1 hit.
    PF00595. PDZ. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF140482. SSF140482. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: BrainImported.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 390-1570.
      Tissue: Brain.
    4. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-269; TYR-1240 AND SER-1292.

    Entry informationi

    Entry nameiMAST1_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y2H9
    Secondary accession number(s): O00114, Q8N6X0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3