Q9Y2H9 (MAST1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated serine/threonine-protein kinase 1
EC=2.7.11.1

Alternative name(s):
Syntrophin-associated serine/threonine-protein kinase

Gene names

Name:	MAST1
Synonyms:	KIAA0973, SAST

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1570 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins By similarity. UniProtKB Q9R1L5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9BXM7
Cofactor	Magnesium By similarity. UniProtKB Q9BXM7
Subunit structure	Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2 By similarity. UniProtKB Q9R1L5
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with syntrophins at the cell membrane By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain.
Sequence caution	The sequence AAB51171.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAH27985.2 differs from that shown. Reason: Erroneous initiation. The sequence BAA76817.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB intracellular protein kinase cascade Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EGFR	P00533	2	EBI-3385920,EBI-297353

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1570

1570

Microtubule-associated serine/threonine-protein kinase 1

PRO_0000086309

Regions

Domain

374 – 647

274

Protein kinase

Domain

648 – 719

72

AGC-kinase C-terminal

Domain

967 – 1055

89

PDZ

Nucleotide binding

380 – 388

9

ATP By similarity UniProtKB Q9BXM7

Sites

Active site

497

1

Proton acceptor By similarity UniProtKB Q9BXM7

Binding site

403

1

ATP By similarity UniProtKB Q9BXM7

Natural variations

Natural variant

269

1

A → T in a metastatic melanoma sample; somatic mutation. Ref.4

VAR_040768

Natural variant

1048

1

A → S.
Corresponds to variant rs35052801 [ dbSNP | Ensembl ].

VAR_051644

Natural variant

1240

1

H → Y in an ovarian serous carcinoma sample; somatic mutation. Ref.4

VAR_040769

Natural variant

1292

1

P → S. Ref.4
Corresponds to variant rs35071862 [ dbSNP | Ensembl ].

VAR_040770

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1570

Helix Strand Turn

Details...

Beta strand

968 – 971

4

Beta strand

973 – 975

3

Beta strand

978 – 987

10

Beta strand

994 – 1003

10

Helix

1008 – 1012

5

Beta strand

1019 – 1023

5

Helix

1033 – 1042

10

Turn

1043 – 1045

3

Beta strand

1046 – 1052

7

Sequences

	Sequence		Length	Mass (Da)
	Q9Y2H9 [UniParc]. Last modified March 1, 2005. Version 2. Checksum: 189D24AE07B62A1E	FASTA	1,570	170,677
	10 20 30 40 50 60 MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH SPLPGHLGSS 70 80 90 100 110 120 PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG TNTPSSTVSS SCSSQERLHQ 130 140 150 160 170 180 LPYQPTVDEL HFLSKHFGST ESITDEDGGR RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH 190 200 210 220 230 240 VYKERFPKAT AQMEEKLRDF TRAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI 250 260 270 280 290 300 TTVYFYELQE NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY 310 320 330 340 350 360 HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVH LEEQDSGGSN TPEQDDLSEG 370 380 390 400 410 420 RSSKAKKPPG ENDFDTIKLI SNGAYGAVYL VRHRDTRQRF AMKKINKQNL ILRNQIQQAF 430 440 450 460 470 480 VERDILTFAE NPFVVGMFCS FETRRHLCMV MEYVEGGDCA TLLKNIGALP VEMARMYFAE 490 500 510 520 530 540 TVLALEYLHN YGIVHRDLKP DNLLITSMGH IKLTDFGLSK MGLMSLTTNL YEGHIEKDAR 550 560 570 580 590 600 EFLDKQVCGT PEYIAPEVIL RQGYGKPVDW WAMGIILYEF LVGCVPFFGD TPEELFGQVI 610 620 630 640 650 660 SDDILWPEGD EALPTEAQLL ISSLLQTNPL VRLGAGGAFE VKQHSFFRDL DWTGLLRQKA 670 680 690 700 710 720 EFIPHLESED DTSYFDTRSD RYHHVNSYDE DDTTEEEPVE IRQFSSCSPR FSKVYSSMEQ 730 740 750 760 770 780 LSQHEPKTPV AAAGSSKREP STKGPEEKVA GKREGLGGLT LREKTWRGGS PEIKRFSASE 790 800 810 820 830 840 ASFLEGEASP PLGARRRFSA LLEPSRFSAP QEDEDEARLR RPPRPSSDPA GSLDARAPKE 850 860 870 880 890 900 ETQGEGTSSA GDSEATDRPR PGDLCPPSKD GDASGPRATN DLVLRRARHQ QMSGDVAVEK 910 920 930 940 950 960 RPSRTGGKVI KSASATALSV MIPAVDPHGS SPLASPMSPR SLSSNPSSRD SSPSRDYSPA 970 980 990 1000 1010 1020 VSGLRSPITI QRSGKKYGFT LRAIRVYMGD TDVYSVHHIV WHVEEGGPAQ EAGLCAGDLI 1030 1040 1050 1060 1070 1080 THVNGEPVHG MVHPEVVELI LKSGNKVAVT TTPFENTSIR IGPARRSSYK AKMARRNKRP 1090 1100 1110 1120 1130 1140 SAKEGQESKK RSSLFRKITK QSNLLHTSRS LSSLNRSLSS SDSLPGSPTH GLPARSPTHS 1150 1160 1170 1180 1190 1200 YRSTPDSAYL GASSQSSSPA SSTPNSPASS ASHHIRPSTL HGLSPKLHRQ YRSARCKSAG 1210 1220 1230 1240 1250 1260 NIPLSPLAHT PSPTQASPPP LPGHTVGSSH TTQSFPAKLH SSPPVVRPRP KSAEPPRSPL 1270 1280 1290 1300 1310 1320 LKRVQSAEKL GASLSADKKG ALRKHSLEVG HPDFRKDFHG ELALHSLAES DGETPPVEGL 1330 1340 1350 1360 1370 1380 GAPRQVAVRR LGRQESPLSL GADPLLPEGA SRPPVSSKEK ESPGGAEACT PPRATTPGGR 1390 1400 1410 1420 1430 1440 TLERDVGCTR HQSVQTEDGT GGMARAVAKA ALSPVQEHET GRRSSSGEAG TPLVPIVVEP 1450 1460 1470 1480 1490 1500 ARPGAKAVVP QPLGADSKGL QEPAPLAPSV PEAPRGRERW VLEVVEERTT LSGPRSKPAS 1510 1520 1530 1540 1550 1560 PKLSPEPQTP SLAPAKCSAP SSAVTPVPPA SLLGSGTKPQ VGLTSRCPAE AVPPAGLTKK 1570 GVSSPAPPGP

References

[1]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 390-1570. Tissue: Brain.
[4]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-269; TYR-1240 AND SER-1292.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB023190 mRNA. Translation: BAA76817.1. Different initiation.
AC020934 Genomic DNA. No translation available.
AD000092 Genomic DNA. Translation: AAB51171.1. Sequence problems.
BC027985 mRNA. Translation: AAH27985.2. Different initiation.

IPI

IPI00027883.

PIR

T45070.

RefSeq

NP_055790.1. NM_014975.2.

UniGene

Hs.227489.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3PS4	X-ray	1.85	A/B/C/D	965-1057	[»]

ProteinModelPortal

Q9Y2H9.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y2H9. 32 interactions.

STRING

9606.ENSP00000251472.

PTM databases

PhosphoSite

Q9Y2H9.

Polymorphism databases

DMDM

60390226.

Proteomic databases

PaxDb

Q9Y2H9.

PeptideAtlas

Q9Y2H9.

PRIDE

Q9Y2H9.

Protocols and materials databases

DNASU

22983.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000251472; ENSP00000251472; ENSG00000105613.

GeneID

22983.

KEGG

hsa:22983.

UCSC

uc002mvm.3. human.

Organism-specific databases

CTD

22983.

GeneCards

GC19P012949.

HGNC

HGNC:19034. MAST1.

HPA

HPA028181.

MIM

612256. gene.

neXtProt

NX_Q9Y2H9.

PharmGKB

PA134946727.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0515.

HOGENOM

HOG000046662.

HOVERGEN

HBG052414.

InParanoid

Q9Y2H9.

KO

K08789.

OMA

FTRAYEP.

OrthoDB

EOG44F68B.

Gene expression databases

Bgee

Q9Y2H9.

CleanEx

HS_MAST1.

Genevestigator

Q9Y2H9.

GermOnline

ENSG00000105613. Homo sapiens.

Family and domain databases

Gene3D

1.20.1480.20. 1 hit.

InterPro

IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

Pfam

PF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.
SSF50156. PDZ. 1 hit.
SSF140482. SSF140482. 1 hit.

PROSITE

PS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q9Y2H9.

ChEMBL

CHEMBL1163128.

ChiTaRS

MAST1. human.

EvolutionaryTrace

Q9Y2H9.

GenomeRNAi

22983.

NextBio

43815.

SOURCE

Search...

Entry information

Entry name

MAST1_HUMAN

Accession

Primary (citable) accession number: Q9Y2H9
Secondary accession number(s): O00114, Q8N6X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.