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Q9Y2H2 (SAC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositide phosphatase SAC2

EC=3.1.3.-
Alternative name(s):
Inositol polyphosphate 5-phosphatase F
Sac domain-containing inositol phosphatase 2
Sac domain-containing phosphoinositide 5-phosphatase 2
Short name=hSAC2
Gene names
Name:INPP5F
Synonyms:KIAA0966, SAC2
ORF Names:MSTP007, MSTP047
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1132 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inositol 5-phosphatase which acts on phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Hydrolyzes phosphatidylinositol 4,5-bisphosphate most effectively. Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation. Ref.1 Ref.9

Tissue specificity

Ubiquitous. Ref.1

Miscellaneous

Inositol polyphosphate 5-phosphatases usually have 2 conserved motifs that are essential for 5-phosphatase activity. However, INPP5F does not contain such motifs, and phosphatase activity seems to exist in the SAC domain, suggesting a new type of phosphoinositide 5-phosphatase.

Sequence similarities

Contains 1 SAC domain.

Biophysicochemical properties

Kinetic parameters:

KM=14.3 µM for PtdIns(4,5)P2 (at pH 6.0) Ref.1

Sequence caution

The sequence BAA76810.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y2H2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y2H2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     373-375: VII → QQK
     376-1132: Missing.
Isoform 3 (identifier: Q9Y2H2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     206-219: DDRFFWNKYMIQDL → PLTARRAGFALGKK
     220-1132: Missing.
Isoform 4 (identifier: Q9Y2H2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MELFQAKDHYILQQGERAL → MCHDVIFMAWLKQQFSECT
     20-629: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11321132Phosphatidylinositide phosphatase SAC2
PRO_0000331621

Regions

Domain167 – 518352SAC

Natural variations

Alternative sequence1 – 1919MELFQ…GERAL → MCHDVIFMAWLKQQFSECT in isoform 4.
VSP_046366
Alternative sequence20 – 629610Missing in isoform 4.
VSP_046367
Alternative sequence206 – 21914DDRFF…MIQDL → PLTARRAGFALGKK in isoform 3.
VSP_033266
Alternative sequence220 – 1132913Missing in isoform 3.
VSP_033267
Alternative sequence373 – 3753VII → QQK in isoform 2.
VSP_033268
Alternative sequence376 – 1132757Missing in isoform 2.
VSP_033269
Natural variant4531I → V.
Corresponds to variant rs3736822 [ dbSNP | Ensembl ].
VAR_042907
Natural variant9971N → D. Ref.2 Ref.6
Corresponds to variant rs3188055 [ dbSNP | Ensembl ].
VAR_042908

Experimental info

Mutagenesis4601D → A: Loss of phosphatase activity. Ref.1
Sequence conflict3861I → V in AAI11494. Ref.6
Sequence conflict6541Y → N in BAG52363. Ref.3
Sequence conflict10081P → L in BAG52363. Ref.3
Sequence conflict10191S → P in BAG52363. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 29, 2008. Version 3.
Checksum: 853719FC0AD455CD

FASTA1,132128,407
        10         20         30         40         50         60 
MELFQAKDHY ILQQGERALW CSRRDGGLQL RPATDLLLAW NPICLGLVEG VIGKIQLHSD 

        70         80         90        100        110        120 
LPWWLILIRQ KALVGKLPGD HEVCKVTKIA VLSLSEMEPQ DLELELCKKH HFGINKPEKI 

       130        140        150        160        170        180 
IPSPDDSKFL LKTFTHIKSN VSAPNKKKVK ESKEKEKLER RLLEELLKMF MDSESFYYSL 

       190        200        210        220        230        240 
TYDLTNSVQR QSTGERDGRP LWQKVDDRFF WNKYMIQDLT EIGTPDVDFW IIPMIQGFVQ 

       250        260        270        280        290        300 
IEELVVNYTE SSDDEKSSPE TPPQESTCVD DIHPRFLVAL ISRRSRHRAG MRYKRRGVDK 

       310        320        330        340        350        360 
NGNVANYVET EQLIHVHNHT LSFVQTRGSV PVFWSQVGYR YNPRPRLDRS EKETVAYFCA 

       370        380        390        400        410        420 
HFEEQLNIYK KQVIINLVDQ AGREKIIGDA YLKQVLLFNN SHLTYVSFDF HEHCRGMKFE 

       430        440        450        460        470        480 
NVQTLTDAIY DIILDMKWCW VDEAGVICKQ EGIFRVNCMD CLDRTNVVQA AIARVVMEQQ 

       490        500        510        520        530        540 
LKKLGVMPPE QPLPVKCNRI YQIMWANNGD SISRQYAGTA ALKGDFTRTG ERKLAGVMKD 

       550        560        570        580        590        600 
GVNSANRYYL NRFKDAYRQA VIDLMQGIPV TEDLYSIFTK EKEHEALHKE NQRSHQELIS 

       610        620        630        640        650        660 
QLLQSYMKLL LPDDEKFHGG WALIDCDPSL IDATHRDVDV LLLLSNSAYY VAYYDDEVDK 

       670        680        690        700        710        720 
VNQYQRLSLE NLEKIEIGPE PTLFGKPKFS CMRLHYRYKE ASGYFHTLRA VMRNPEEDGK 

       730        740        750        760        770        780 
DTLQCIAEML QITKQAMGSD LPIIEKKLER KSSKPHEDII GIRSQNQGSL AQGKNFLMSK 

       790        800        810        820        830        840 
FSSLNQKVKQ TKSNVNIGNL RKLGNFTKPE MKVNFLKPNL KVNLWKSDSS LETMENTGVM 

       850        860        870        880        890        900 
DKVQAESDGD MSSDNDSYHS DEFLTNSKSD EDRQLANSLE SVGPIDYVLP SCGIIASAPR 

       910        920        930        940        950        960 
LGSRSQSLSS TDSSVHAPSE ITVAHGSGLG KGQESPLKKS PSAGDVHILT GFAKPMDIYC 

       970        980        990       1000       1010       1020 
HRFVQDAQNK VTHLSETRSV SQQASQERNQ MTNQVSNETQ SESTEQTPSR PSQLDVSLSA 

      1030       1040       1050       1060       1070       1080 
TGPQFLSVEP AHSVASQKTP TSASSMLELE TGLHVTPSPS ESSSSRAVSP FAKIRSSMVQ 

      1090       1100       1110       1120       1130 
VASITQAGLT HGINFAVSKV QKSPPEPEII NQVQQNELKK MFIQCQTRII QI 

« Hide

Isoform 2 [UniParc].

Checksum: E9A76393F10C6CC3
Show »

FASTA37543,817
Isoform 3 [UniParc].

Checksum: 8AAA498682414245
Show »

FASTA21925,174
Isoform 4 [UniParc].

Checksum: 8A15030B00C8E9A1
Show »

FASTA52257,800

References

« Hide 'large scale' references
[1]"Identification and characterization of a sac domain-containing phosphoinositide 5-phosphatase."
Minagawa T., Ijuin T., Mochizuki Y., Takenawa T.
J. Biol. Chem. 276:22011-22015(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ASP-460, TISSUE SPECIFICITY.
[2]"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-997.
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Brain.
[4]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), VARIANT ASP-997.
Tissue: Placenta and Skin.
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 274-1132 (ISOFORM 1).
Tissue: Testis.
[8]Liu B., Liu Y.Q., Wang X.Y., Zhao B., Sheng H., Zhao X.W., Liu S., Xu Y.Y., Ye J., Song L., Gao Y., Zhang C.L., Zhang J., Wei Y.J., Cao H.Q., Zhao Y., Liu L.S., Ding J.F. expand/collapse author list , Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 956-1132 (ISOFORM 1).
Tissue: Aorta.
[9]"Hdac2 regulates the cardiac hypertrophic response by modulating Gsk3 beta activity."
Trivedi C.M., Luo Y., Yin Z., Zhang M., Zhu W., Wang T., Floss T., Goettlicher M., Noppinger P.R., Wurst W., Ferrari V.A., Abrams C.S., Gruber P.J., Epstein J.A.
Nat. Med. 13:324-331(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023183 mRNA. Translation: BAA76810.2. Different initiation.
AK091448 mRNA. Translation: BAG52363.1.
AC027672 Genomic DNA. No translation available.
AL133461, AL158014 Genomic DNA. Translation: CAH72974.1.
AL158014, AL133461 Genomic DNA. Translation: CAI16957.1.
CH471066 Genomic DNA. Translation: EAW49379.1.
CH471066 Genomic DNA. Translation: EAW49380.1.
CH471066 Genomic DNA. Translation: EAW49381.1.
BC052367 mRNA. Translation: AAH52367.1.
BC067820 mRNA. Translation: AAH67820.1.
BC082755 mRNA. Translation: AAH82755.1.
BC111493 mRNA. Translation: AAI11494.1.
AL137528 mRNA. Translation: CAB70792.1.
AF113227 mRNA. Translation: AAG39298.1.
AF109361 mRNA. Translation: AAQ13509.1.
PIRT46372.
RefSeqNP_001230123.1. NM_001243194.1.
NP_001230124.1. NM_001243195.1.
NP_055752.1. NM_014937.3.
UniGeneHs.369755.

3D structure databases

ProteinModelPortalQ9Y2H2.
SMRQ9Y2H2. Positions 5-520.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116543. 1 interaction.
IntActQ9Y2H2. 1 interaction.
STRING9606.ENSP00000354519.

PTM databases

PhosphoSiteQ9Y2H2.

Polymorphism databases

DMDM187611527.

Proteomic databases

PaxDbQ9Y2H2.
PRIDEQ9Y2H2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361976; ENSP00000354519; ENSG00000198825. [Q9Y2H2-1]
ENST00000369080; ENSP00000358076; ENSG00000198825. [Q9Y2H2-4]
ENST00000369083; ENSP00000358079; ENSG00000198825. [Q9Y2H2-2]
GeneID22876.
KEGGhsa:22876.
UCSCuc001len.4. human. [Q9Y2H2-3]
uc001leo.3. human. [Q9Y2H2-1]

Organism-specific databases

CTD22876.
GeneCardsGC10P121475.
HGNCHGNC:17054. INPP5F.
HPAHPA042605.
MIM609389. gene.
neXtProtNX_Q9Y2H2.
PharmGKBPA134927878.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5329.
HOVERGENHBG095361.
InParanoidQ9Y2H2.
OMAENTGVMD.
OrthoDBEOG73803W.
PhylomeDBQ9Y2H2.
TreeFamTF313543.

Enzyme and pathway databases

BioCycMetaCyc:HS12255-MONOMER.

Gene expression databases

ArrayExpressQ9Y2H2.
BgeeQ9Y2H2.
CleanExHS_INPP5F.
GenevestigatorQ9Y2H2.

Family and domain databases

InterProIPR022158. Inositol_phosphatase.
IPR002013. Syja_N.
[Graphical view]
PfamPF12456. hSac2. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
PROSITEPS50275. SAC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi22876.
NextBio35469521.
PROQ9Y2H2.
SOURCESearch...

Entry information

Entry nameSAC2_HUMAN
AccessionPrimary (citable) accession number: Q9Y2H2
Secondary accession number(s): B3KRF1 expand/collapse secondary AC list , D3DRD1, Q2T9J4, Q5W135, Q5W136, Q6NVY2, Q86U97, Q9H3D9, Q9NT51
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM