Reviewed, UniProtKB/Swiss-Prot Q9Y2H0 (DLGP4_HUMAN)

Names and origin

Protein names

Recommended name:
    Disks large-associated protein 4
      Short name=DAP-4
Alternative name(s):
    SAP90/PSD-95-associated protein 4
      Short name=SAPAP-4
    PSD-95/SAP90-binding protein 4

Gene names

Name:	DLGAP4
Synonyms:	DAP4, KIAA0964, SAPAP4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	992 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.
Subunit structure	Interacts with DLG1 and DLG4/PSD-95 By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the SAPAP family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Inferred from electronic annotation. Source: InterPro
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABL1	P00519	1	EBI-722139,EBI-375543
BIN1	O00499	3	EBI-722139,EBI-719094
CRK	P46108	1	EBI-722139,EBI-886
FYN	P06241	1	EBI-722139,EBI-515315
GRB2	P62993	1	EBI-722139,EBI-401755
NCK1	P16333	1	EBI-722139,EBI-389883
PIK3R1	P27986	1	EBI-722139,EBI-79464
SRC	P12931	1	EBI-722139,EBI-621482

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2H0-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2H0-1)

The sequence of this isoform differs from the canonical sequence as follows:
     671-700: VDCIQPVPKEEPSPATKFQSIGVQVEDDWR → ERTRRNGSHLSEDNGPKAIDVMAPSSE

Isoform 3 (identifier: Q9Y2H0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-539: Missing.
     540-550: GSLSNSRTLPS → MALCLELLKQC

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 992	992	Disks large-associated protein 4		PRO_0000174297
Regions
	Compositional bias	267 – 274	8	Poly-Pro
Amino acid modifications
	Modified residue	368	1	Phosphoserine By similarity
	Modified residue	369	1	Phosphotyrosine Ref.6
	Modified residue	378	1	Phosphoserine By similarity
	Modified residue	397	1	Phosphoserine By similarity
	Modified residue	421	1	Phosphoserine By similarity
	Modified residue	512	1	Phosphoserine By similarity
	Modified residue	665	1	Phosphoserine By similarity
	Modified residue	915	1	Phosphothreonine Ref.7
	Modified residue	973	1	Phosphoserine By similarity
	Modified residue	984	1	Phosphotyrosine Ref.5
Natural variations
	Alternative sequence	1 – 539	539	Missing in isoform 3.		VSP_034910
	Alternative sequence	540 – 550	11	GSLSNSRTLPS → MALCLELLKQC in isoform 3.		VSP_034911
	Alternative sequence	671 – 700	30	VDCIQ…EDDWR → ERTRRNGSHLSEDNGPKAID VMAPSSE in isoform 2.		VSP_006013
	Natural variant	486	1	A → T: dbSNP rs6019652.		VAR_057716
	Natural variant	861	1	R → Q: dbSNP rs2275807.		VAR_057717
Experimental info
	Sequence conflict	229	1	T → I in BAA76808. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 22, 2008. Version 3. Checksum: F0EBFBE0DE1AC814	FASTA	992	108,012
	10 20 30 40 50 60 MKGLGDSRPR HLSDSLDPPH EPLFAGTDRN PYLLSPTEAF AREARFPGQN TLPGDGLFPL 70 80 90 100 110 120 NNQLPPPSST FPRIHYNSHF EVPEESPFPS HAQATKINRL PANLLDQFEK QLPIHRDGFS 130 140 150 160 170 180 TLQFPRGEAK ARGESPGRIR HLVHSVQRLF FTKAPSLEGT AGKVGGNGSK KGGMEDGKGR 190 200 210 220 230 240 RAKSKERAKA GEPKRRSRSN ISGWWSSDDN LDGEAGAFRS SGPASGLMTL GRQAERSQPR 250 260 270 280 290 300 YFMHAYNTIS GHMLKTTKNN TTELTAPPPP PAPPATCPSL GVGTDTNYVK RGSWSTLTLS 310 320 330 340 350 360 HAHEVCQKTS ATLDKSLLKS KSCHQGLAYH YLQVPGGGGE WSTTLLSPRE TDAAAEGPIP 370 380 390 400 410 420 CRRMRSGSYI KAMGDEDSDE SGGSPKPSPK TAARRQSYLR ATQQSLGEQS NPRRSLDRLD 430 440 450 460 470 480 SVDMLLPSKC PSWEEDYTPV SDSLNDSSCI SQIFGQASLI PQLFGHEQQV REAELSDQYE 490 500 510 520 530 540 AACESACSEA ESTAAETLDL PLPSYFRSRS HSYLRAIQAG CSQEEDSVSL QSLSPPPSTG 550 560 570 580 590 600 SLSNSRTLPS SSCLVAYKKT PPPVPPRTTS KPFISVTVQS STESAQDTYL DSQDHKSEVT 610 620 630 640 650 660 SQSGLSNSSD SLDSSTRPPS VTRGGVAPAP EAPEPPPKHA ALKSEQGTLT SSESHPEAAP 670 680 690 700 710 720 KRKLSSIGIQ VDCIQPVPKE EPSPATKFQS IGVQVEDDWR SSVPSHSMSS RRDTDSDTQD 730 740 750 760 770 780 ANDSSCKSSE RSLPDCTPHP NSISIDAGPR QAPKIAQIKR NLSYGDNSDP ALEASSLPPP 790 800 810 820 830 840 DPWLETSSSS PAEPAQPGAC RRDGYWFLKL LQAETERLEG WCCQMDKETK ENNLSEEVLG 850 860 870 880 890 900 KVLSAVGSAQ LLMSQKFQQF RGLCEQNLNP DANPRPTAQD LAGFWDLLQL SIEDISMKFD 910 920 930 940 950 960 ELYHLKANSW QLVETPEKRK EEKKPPPPVP KKPAKSKPAV SRDKASDASD KQRQEARKRL 970 980 990 LAAKRAASVR QNSATESADS IEIYVPEAQT RL
	Isoform 2. Checksum: B2FD9A2103E052EB	FASTA	989	107,567
	10 20 30 40 50 60 MKGLGDSRPR HLSDSLDPPH EPLFAGTDRN PYLLSPTEAF AREARFPGQN TLPGDGLFPL 70 80 90 100 110 120 NNQLPPPSST FPRIHYNSHF EVPEESPFPS HAQATKINRL PANLLDQFEK QLPIHRDGFS 130 140 150 160 170 180 TLQFPRGEAK ARGESPGRIR HLVHSVQRLF FTKAPSLEGT AGKVGGNGSK KGGMEDGKGR 190 200 210 220 230 240 RAKSKERAKA GEPKRRSRSN ISGWWSSDDN LDGEAGAFRS SGPASGLMTL GRQAERSQPR 250 260 270 280 290 300 YFMHAYNTIS GHMLKTTKNN TTELTAPPPP PAPPATCPSL GVGTDTNYVK RGSWSTLTLS 310 320 330 340 350 360 HAHEVCQKTS ATLDKSLLKS KSCHQGLAYH YLQVPGGGGE WSTTLLSPRE TDAAAEGPIP 370 380 390 400 410 420 CRRMRSGSYI KAMGDEDSDE SGGSPKPSPK TAARRQSYLR ATQQSLGEQS NPRRSLDRLD 430 440 450 460 470 480 SVDMLLPSKC PSWEEDYTPV SDSLNDSSCI SQIFGQASLI PQLFGHEQQV REAELSDQYE 490 500 510 520 530 540 AACESACSEA ESTAAETLDL PLPSYFRSRS HSYLRAIQAG CSQEEDSVSL QSLSPPPSTG 550 560 570 580 590 600 SLSNSRTLPS SSCLVAYKKT PPPVPPRTTS KPFISVTVQS STESAQDTYL DSQDHKSEVT 610 620 630 640 650 660 SQSGLSNSSD SLDSSTRPPS VTRGGVAPAP EAPEPPPKHA ALKSEQGTLT SSESHPEAAP 670 680 690 700 710 720 KRKLSSIGIQ ERTRRNGSHL SEDNGPKAID VMAPSSESSV PSHSMSSRRD TDSDTQDAND 730 740 750 760 770 780 SSCKSSERSL PDCTPHPNSI SIDAGPRQAP KIAQIKRNLS YGDNSDPALE ASSLPPPDPW 790 800 810 820 830 840 LETSSSSPAE PAQPGACRRD GYWFLKLLQA ETERLEGWCC QMDKETKENN LSEEVLGKVL 850 860 870 880 890 900 SAVGSAQLLM SQKFQQFRGL CEQNLNPDAN PRPTAQDLAG FWDLLQLSIE DISMKFDELY 910 920 930 940 950 960 HLKANSWQLV ETPEKRKEEK KPPPPVPKKP AKSKPAVSRD KASDASDKQR QEARKRLLAA 970 980 KRAASVRQNS ATESADSIEI YVPEAQTRL
	Isoform 3. Checksum: 877C20CA747800EB	FASTA	453	49,478
	10 20 30 40 50 60 MALCLELLKQ CSSCLVAYKK TPPPVPPRTT SKPFISVTVQ SSTESAQDTY LDSQDHKSEV 70 80 90 100 110 120 TSQSGLSNSS DSLDSSTRPP SVTRGGVAPA PEAPEPPPKH AALKSEQGTL TSSESHPEAA 130 140 150 160 170 180 PKRKLSSIGI QVDCIQPVPK EEPSPATKFQ SIGVQVEDDW RSSVPSHSMS SRRDTDSDTQ 190 200 210 220 230 240 DANDSSCKSS ERSLPDCTPH PNSISIDAGP RQAPKIAQIK RNLSYGDNSD PALEASSLPP 250 260 270 280 290 300 PDPWLETSSS SPAEPAQPGA CRRDGYWFLK LLQAETERLE GWCCQMDKET KENNLSEEVL 310 320 330 340 350 360 GKVLSAVGSA QLLMSQKFQQ FRGLCEQNLN PDANPRPTAQ DLAGFWDLLQ LSIEDISMKF 370 380 390 400 410 420 DELYHLKANS WQLVETPEKR KEEKKPPPPV PKKPAKSKPA VSRDKASDAS DKQRQEARKR 430 440 450 LLAAKRAASV RQNSATESAD SIEIYVPEAQ TRL

References

[1]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed: 10231032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: PNS.
[5]	"Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling." Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A., Lottspeich F., Chen Z. EMBO J. 25:5058-5070(2006) [PubMed: 17053785] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-984, MASS SPECTROMETRY.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-369, MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-915, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB023181 mRNA. Translation: BAA76808.2. Different initiation. AL390374, AL050318 Genomic DNA. Translation: CAI17927.1. AL390374, AL050318 Genomic DNA. Translation: CAI17928.1. AL050318, AL390374 Genomic DNA. Translation: CAI23506.1. AL050318, AL390374 Genomic DNA. Translation: CAI23507.1. AL390374, AL050318 Genomic DNA. Translation: CAI17929.1. AL050318, AL390374 Genomic DNA. Translation: CAI23509.1. CH471077 Genomic DNA. Translation: EAW76140.1. BC108706 mRNA. Translation: AAI08707.1. BC153874 mRNA. Translation: AAI53875.1.
IPI	IPI00007138. IPI00220581. IPI00411461.
RefSeq	NP_001035951.1. NP_055717.2. NP_892118.1.
UniGene	Hs.249600
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y2H0. 11 interactions.
STRING	Q9Y2H0.
PTM databases
PhosphoSite	Q9Y2H0.
Proteomic databases
PRIDE	Q9Y2H0.
Genome annotation databases
Ensembl	ENST00000339266; ENSP00000341633; ENSG00000080845; Homo sapiens. [Genome view] ENST00000340491; ENSP00000345700; ENSG00000080845; Homo sapiens. [Genome view] ENST00000341277; ENSP00000345630; ENSG00000080845; Homo sapiens. [Genome view] ENST00000373907; ENSP00000363014; ENSG00000080845; Homo sapiens. [Genome view] ENST00000373913; ENSP00000363023; ENSG00000080845; Homo sapiens. [Genome view] ENST00000401952; ENSP00000384954; ENSG00000080845; Homo sapiens. [Genome view] ENST00000427554; ENSP00000413923; ENSG00000080845; Homo sapiens. [Genome view]
GeneID	22839.
UCSC	uc002xff.1. human. uc002xfg.1. human.
Organism-specific databases
CTD	22839.
GeneCards	GC20P034429.
H-InvDB	HIX0015778.
HGNC	HGNC:24476. DLGAP4.
PharmGKB	PA134891458.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9Y2H0.
OMA	AYHYLQV.
Gene expression databases
ArrayExpress	Q9Y2H0.
Bgee	Q9Y2H0.
CleanEx	HS_DLGAP4.
Genevestigator	Q9Y2H0.
GermOnline	ENSG00000080845. Homo sapiens.
Family and domain databases
InterPro	IPR005026. GKAP. [Graphical view]
Pfam	PF03359. GKAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	43281.

Entry information

Entry name

DLGP4_HUMAN

Accession

Primary (citable) accession number: Q9Y2H0
Secondary accession number(s): Q5QPG4 , Q5T2Y4, Q5T2Y5, Q9H137, Q9H138, Q9H1L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.