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Protein

Disks large-associated protein 4

Gene

DLGAP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 4
Short name:
DAP-4
Alternative name(s):
PSD-95/SAP90-binding protein 4
SAP90/PSD-95-associated protein 4
Short name:
SAPAP-4
Gene namesi
Name:DLGAP4
Synonyms:DAP4, KIAA0964, SAPAP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:24476. DLGAP4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134891458.

Polymorphism and mutation databases

BioMutaiDLGAP4.
DMDMi205831580.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 992992Disks large-associated protein 4PRO_0000174297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei378 – 3781PhosphoserineBy similarity
Modified residuei915 – 9151Phosphothreonine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Y2H0.
PaxDbiQ9Y2H0.
PRIDEiQ9Y2H0.

PTM databases

PhosphoSiteiQ9Y2H0.

Expressioni

Gene expression databases

BgeeiQ9Y2H0.
CleanExiHS_DLGAP4.
ExpressionAtlasiQ9Y2H0. baseline and differential.
GenevisibleiQ9Y2H0. HS.

Organism-specific databases

HPAiHPA054105.
HPA056378.

Interactioni

Subunit structurei

Interacts with DLG1 and DLG4/PSD-95.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BIN1O004994EBI-722139,EBI-719094
GRB2P629932EBI-722139,EBI-401755
NCK1P163335EBI-722139,EBI-389883
PIK3R1P279862EBI-722139,EBI-79464

Protein-protein interaction databases

BioGridi116513. 10 interactions.
IntActiQ9Y2H0. 14 interactions.
MINTiMINT-8105041.
STRINGi9606.ENSP00000363023.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2H0.
SMRiQ9Y2H0. Positions 803-908.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi267 – 2748Poly-Pro

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Phylogenomic databases

eggNOGiNOG331770.
GeneTreeiENSGT00550000074473.
HOVERGENiHBG018957.
InParanoidiQ9Y2H0.
OMAiPSWEEDY.
PhylomeDBiQ9Y2H0.
TreeFamiTF321382.

Family and domain databases

InterProiIPR030527. DLGAP4.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF19. PTHR12353:SF19. 1 hit.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2H0-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGLGDSRPR HLSDSLDPPH EPLFAGTDRN PYLLSPTEAF AREARFPGQN
60 70 80 90 100
TLPGDGLFPL NNQLPPPSST FPRIHYNSHF EVPEESPFPS HAQATKINRL
110 120 130 140 150
PANLLDQFEK QLPIHRDGFS TLQFPRGEAK ARGESPGRIR HLVHSVQRLF
160 170 180 190 200
FTKAPSLEGT AGKVGGNGSK KGGMEDGKGR RAKSKERAKA GEPKRRSRSN
210 220 230 240 250
ISGWWSSDDN LDGEAGAFRS SGPASGLMTL GRQAERSQPR YFMHAYNTIS
260 270 280 290 300
GHMLKTTKNN TTELTAPPPP PAPPATCPSL GVGTDTNYVK RGSWSTLTLS
310 320 330 340 350
HAHEVCQKTS ATLDKSLLKS KSCHQGLAYH YLQVPGGGGE WSTTLLSPRE
360 370 380 390 400
TDAAAEGPIP CRRMRSGSYI KAMGDEDSDE SGGSPKPSPK TAARRQSYLR
410 420 430 440 450
ATQQSLGEQS NPRRSLDRLD SVDMLLPSKC PSWEEDYTPV SDSLNDSSCI
460 470 480 490 500
SQIFGQASLI PQLFGHEQQV REAELSDQYE AACESACSEA ESTAAETLDL
510 520 530 540 550
PLPSYFRSRS HSYLRAIQAG CSQEEDSVSL QSLSPPPSTG SLSNSRTLPS
560 570 580 590 600
SSCLVAYKKT PPPVPPRTTS KPFISVTVQS STESAQDTYL DSQDHKSEVT
610 620 630 640 650
SQSGLSNSSD SLDSSTRPPS VTRGGVAPAP EAPEPPPKHA ALKSEQGTLT
660 670 680 690 700
SSESHPEAAP KRKLSSIGIQ VDCIQPVPKE EPSPATKFQS IGVQVEDDWR
710 720 730 740 750
SSVPSHSMSS RRDTDSDTQD ANDSSCKSSE RSLPDCTPHP NSISIDAGPR
760 770 780 790 800
QAPKIAQIKR NLSYGDNSDP ALEASSLPPP DPWLETSSSS PAEPAQPGAC
810 820 830 840 850
RRDGYWFLKL LQAETERLEG WCCQMDKETK ENNLSEEVLG KVLSAVGSAQ
860 870 880 890 900
LLMSQKFQQF RGLCEQNLNP DANPRPTAQD LAGFWDLLQL SIEDISMKFD
910 920 930 940 950
ELYHLKANSW QLVETPEKRK EEKKPPPPVP KKPAKSKPAV SRDKASDASD
960 970 980 990
KQRQEARKRL LAAKRAASVR QNSATESADS IEIYVPEAQT RL
Length:992
Mass (Da):108,012
Last modified:July 22, 2008 - v3
Checksum:iF0EBFBE0DE1AC814
GO
Isoform 2 (identifier: Q9Y2H0-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     671-700: VDCIQPVPKEEPSPATKFQSIGVQVEDDWR → ERTRRNGSHLSEDNGPKAIDVMAPSSE

Show »
Length:989
Mass (Da):107,567
Checksum:iB2FD9A2103E052EB
GO
Isoform 3 (identifier: Q9Y2H0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-539: Missing.
     540-550: GSLSNSRTLPS → MALCLELLKQC

Show »
Length:453
Mass (Da):49,478
Checksum:i877C20CA747800EB
GO

Sequence cautioni

The sequence BAA76808.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti229 – 2291T → I in BAA76808 (PubMed:10231032).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti486 – 4861A → T.
Corresponds to variant rs6019652 [ dbSNP | Ensembl ].
VAR_057716
Natural varianti861 – 8611R → Q.
Corresponds to variant rs2275807 [ dbSNP | Ensembl ].
VAR_057717

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 539539Missing in isoform 3. 1 PublicationVSP_034910Add
BLAST
Alternative sequencei540 – 55011GSLSNSRTLPS → MALCLELLKQC in isoform 3. 1 PublicationVSP_034911Add
BLAST
Alternative sequencei671 – 70030VDCIQ…EDDWR → ERTRRNGSHLSEDNGPKAID VMAPSSE in isoform 2. 1 PublicationVSP_006013Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023181 mRNA. Translation: BAA76808.2. Different initiation.
AL390374, AL050318 Genomic DNA. Translation: CAI17927.1.
AL390374, AL050318 Genomic DNA. Translation: CAI17928.1.
AL050318, AL390374 Genomic DNA. Translation: CAI23506.1.
AL050318, AL390374 Genomic DNA. Translation: CAI23507.1.
AL390374, AL050318 Genomic DNA. Translation: CAI17929.1.
AL050318, AL390374 Genomic DNA. Translation: CAI23509.1.
CH471077 Genomic DNA. Translation: EAW76140.1.
CH471077 Genomic DNA. Translation: EAW76135.1.
CH471077 Genomic DNA. Translation: EAW76136.1.
BC108706 mRNA. Translation: AAI08707.1.
BC153874 mRNA. Translation: AAI53875.1.
CCDSiCCDS13274.1. [Q9Y2H0-1]
CCDS13275.1. [Q9Y2H0-3]
RefSeqiNP_001035951.1. NM_001042486.3.
NP_055717.2. NM_014902.5. [Q9Y2H0-1]
NP_892118.1. NM_183006.3. [Q9Y2H0-3]
XP_011526990.1. XM_011528688.1. [Q9Y2H0-2]
UniGeneiHs.249600.

Genome annotation databases

EnsembliENST00000339266; ENSP00000341633; ENSG00000080845.
ENST00000340491; ENSP00000345700; ENSG00000080845. [Q9Y2H0-3]
ENST00000373907; ENSP00000363014; ENSG00000080845.
ENST00000373913; ENSP00000363023; ENSG00000080845. [Q9Y2H0-1]
ENST00000401952; ENSP00000384954; ENSG00000080845. [Q9Y2H0-1]
GeneIDi22839.
KEGGihsa:22839.
UCSCiuc002xff.3. human. [Q9Y2H0-1]
uc002xfg.3. human. [Q9Y2H0-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023181 mRNA. Translation: BAA76808.2. Different initiation.
AL390374, AL050318 Genomic DNA. Translation: CAI17927.1.
AL390374, AL050318 Genomic DNA. Translation: CAI17928.1.
AL050318, AL390374 Genomic DNA. Translation: CAI23506.1.
AL050318, AL390374 Genomic DNA. Translation: CAI23507.1.
AL390374, AL050318 Genomic DNA. Translation: CAI17929.1.
AL050318, AL390374 Genomic DNA. Translation: CAI23509.1.
CH471077 Genomic DNA. Translation: EAW76140.1.
CH471077 Genomic DNA. Translation: EAW76135.1.
CH471077 Genomic DNA. Translation: EAW76136.1.
BC108706 mRNA. Translation: AAI08707.1.
BC153874 mRNA. Translation: AAI53875.1.
CCDSiCCDS13274.1. [Q9Y2H0-1]
CCDS13275.1. [Q9Y2H0-3]
RefSeqiNP_001035951.1. NM_001042486.3.
NP_055717.2. NM_014902.5. [Q9Y2H0-1]
NP_892118.1. NM_183006.3. [Q9Y2H0-3]
XP_011526990.1. XM_011528688.1. [Q9Y2H0-2]
UniGeneiHs.249600.

3D structure databases

ProteinModelPortaliQ9Y2H0.
SMRiQ9Y2H0. Positions 803-908.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116513. 10 interactions.
IntActiQ9Y2H0. 14 interactions.
MINTiMINT-8105041.
STRINGi9606.ENSP00000363023.

PTM databases

PhosphoSiteiQ9Y2H0.

Polymorphism and mutation databases

BioMutaiDLGAP4.
DMDMi205831580.

Proteomic databases

MaxQBiQ9Y2H0.
PaxDbiQ9Y2H0.
PRIDEiQ9Y2H0.

Protocols and materials databases

DNASUi22839.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339266; ENSP00000341633; ENSG00000080845.
ENST00000340491; ENSP00000345700; ENSG00000080845. [Q9Y2H0-3]
ENST00000373907; ENSP00000363014; ENSG00000080845.
ENST00000373913; ENSP00000363023; ENSG00000080845. [Q9Y2H0-1]
ENST00000401952; ENSP00000384954; ENSG00000080845. [Q9Y2H0-1]
GeneIDi22839.
KEGGihsa:22839.
UCSCiuc002xff.3. human. [Q9Y2H0-1]
uc002xfg.3. human. [Q9Y2H0-3]

Organism-specific databases

CTDi22839.
GeneCardsiGC20P034894.
HGNCiHGNC:24476. DLGAP4.
HPAiHPA054105.
HPA056378.
neXtProtiNX_Q9Y2H0.
PharmGKBiPA134891458.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG331770.
GeneTreeiENSGT00550000074473.
HOVERGENiHBG018957.
InParanoidiQ9Y2H0.
OMAiPSWEEDY.
PhylomeDBiQ9Y2H0.
TreeFamiTF321382.

Miscellaneous databases

ChiTaRSiDLGAP4. human.
GeneWikiiDLGAP4.
GenomeRNAii22839.
NextBioi43281.
PROiQ9Y2H0.

Gene expression databases

BgeeiQ9Y2H0.
CleanExiHS_DLGAP4.
ExpressionAtlasiQ9Y2H0. baseline and differential.
GenevisibleiQ9Y2H0. HS.

Family and domain databases

InterProiIPR030527. DLGAP4.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF19. PTHR12353:SF19. 1 hit.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: PNS.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-915, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-915, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-915, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDLGP4_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2H0
Secondary accession number(s): E1P5T5
, Q5QPG4, Q5T2Y4, Q5T2Y5, Q9H137, Q9H138, Q9H1L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 22, 2008
Last modified: July 22, 2015
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.