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Protein

Sodium/hydrogen exchanger 8

Gene

SLC9A8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_19314. Sodium/Proton exchangers.

Protein family/group databases

TCDBi2.A.36.1.9. the monovalent cation:proton antiporter-1 (cpa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 8
Alternative name(s):
Na(+)/H(+) exchanger 8
Short name:
NHE-8
Solute carrier family 9 member 8
Gene namesi
Name:SLC9A8
Synonyms:KIAA0939, NHE8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:20728. SLC9A8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Transmembranei84 – 10421HelicalSequence AnalysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST
Transmembranei156 – 17621HelicalSequence AnalysisAdd
BLAST
Transmembranei191 – 21121HelicalSequence AnalysisAdd
BLAST
Transmembranei264 – 28421HelicalSequence AnalysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence AnalysisAdd
BLAST
Transmembranei354 – 37421HelicalSequence AnalysisAdd
BLAST
Transmembranei379 – 39921HelicalSequence AnalysisAdd
BLAST
Transmembranei417 – 43721HelicalSequence AnalysisAdd
BLAST
Transmembranei451 – 47121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134924114.

Polymorphism and mutation databases

BioMutaiSLC9A8.
DMDMi254763448.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Sodium/hydrogen exchanger 8PRO_0000052365Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei571 – 5711Phosphoserine1 Publication
Modified residuei573 – 5731Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Y2E8.
PaxDbiQ9Y2E8.
PRIDEiQ9Y2E8.

PTM databases

PhosphoSiteiQ9Y2E8.

Expressioni

Tissue specificityi

Ubiquitous. Strongly expressed in skeletal muscle and kidney.1 Publication

Gene expression databases

BgeeiQ9Y2E8.
CleanExiHS_SLC9A8.
GenevisibleiQ9Y2E8. HS.

Organism-specific databases

HPAiCAB033682.

Interactioni

Protein-protein interaction databases

BioGridi116906. 1 interaction.
IntActiQ9Y2E8. 1 interaction.
STRINGi9606.ENSP00000354966.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2E8.
SMRiQ9Y2E8. Positions 97-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000172307.
HOVERGENiHBG056815.
InParanoidiQ9Y2E8.
KOiK14724.
OMAiDVIYKMT.
OrthoDBiEOG7H4DT6.
PhylomeDBiQ9Y2E8.
TreeFamiTF354313.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2E8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEKMAEEER FPNTTHEGFN VTLHTTLVVT TKLVLPTPGK PILPVQTGEQ
60 70 80 90 100
AQQEEQSSGM TIFFSLLVLA ICIILVHLLI RYRLHFLPES VAVVSLGILM
110 120 130 140 150
GAVIKIIEFK KLANWKEEEM FRPNMFFLLL LPPIIFESGY SLHKGNFFQN
160 170 180 190 200
IGSITLFAVF GTAISAFVVG GGIYFLGQAD VISKLNMTDS FAFGSLISAV
210 220 230 240 250
DPVATIAIFN ALHVDPVLNM LVFGESILND AVSIVLTNTA EGLTRKNMSD
260 270 280 290 300
VSGWQTFLQA LDYFLKMFFG SAALGTLTGL ISALVLKHID LRKTPSLEFG
310 320 330 340 350
MMIIFAYLPY GLAEGISLSG IMAILFSGIV MSHYTHHNLS PVTQILMQQT
360 370 380 390 400
LRTVAFLCET CVFAFLGLSI FSFPHKFEIS FVIWCIVLVL FGRAVNIFPL
410 420 430 440 450
SYLLNFFRDH KITPKMMFIM WFSGLRGAIP YALSLHLDLE PMEKRQLIGT
460 470 480 490 500
TTIVIVLFTI LLLGGSTMPL IRLMDIEDAK AHRRNKKDVN LSKTEKMGNT
510 520 530 540 550
VESEHLSELT EEEYEAHYIR RQDLKGFVWL DAKYLNPFFT RRLTQEDLHH
560 570 580
GRIQMKTLTN KWYEEVRQGP SGSEDDEQEL L
Length:581
Mass (Da):65,422
Last modified:July 28, 2009 - v4
Checksum:i7508B62B74D584B1
GO
Isoform 2 (identifier: Q9Y2E8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-178: Q → QGFFFVCVCVFVCFILQ

Show »
Length:597
Mass (Da):67,275
Checksum:iF82E64383B1E4192
GO

Sequence cautioni

The sequence BAA76783.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141N → D in BAC85475 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei178 – 1781Q → QGFFFVCVCVFVCFILQ in isoform 2. 1 PublicationVSP_037686

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023156 mRNA. Translation: BAA76783.2. Different initiation.
AK131001 mRNA. Translation: BAC85475.1. Different termination.
AK300318 mRNA. Translation: BAG62070.1.
CR749638 mRNA. Translation: CAH18432.1.
AL162615, AL031685 Genomic DNA. Translation: CAI18970.1.
AL031685, AL162615 Genomic DNA. Translation: CAI23460.1.
CH471077 Genomic DNA. Translation: EAW75649.1.
BC112213 mRNA. Translation: AAI12214.1.
CCDSiCCDS13421.1. [Q9Y2E8-1]
CCDS58774.1. [Q9Y2E8-2]
RefSeqiNP_001247420.1. NM_001260491.1. [Q9Y2E8-2]
NP_056081.1. NM_015266.2. [Q9Y2E8-1]
UniGeneiHs.444202.

Genome annotation databases

EnsembliENST00000361573; ENSP00000354966; ENSG00000197818.
ENST00000417961; ENSP00000416418; ENSG00000197818. [Q9Y2E8-2]
GeneIDi23315.
KEGGihsa:23315.
UCSCiuc002xuv.2. human. [Q9Y2E8-1]
uc010zyj.2. human. [Q9Y2E8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023156 mRNA. Translation: BAA76783.2. Different initiation.
AK131001 mRNA. Translation: BAC85475.1. Different termination.
AK300318 mRNA. Translation: BAG62070.1.
CR749638 mRNA. Translation: CAH18432.1.
AL162615, AL031685 Genomic DNA. Translation: CAI18970.1.
AL031685, AL162615 Genomic DNA. Translation: CAI23460.1.
CH471077 Genomic DNA. Translation: EAW75649.1.
BC112213 mRNA. Translation: AAI12214.1.
CCDSiCCDS13421.1. [Q9Y2E8-1]
CCDS58774.1. [Q9Y2E8-2]
RefSeqiNP_001247420.1. NM_001260491.1. [Q9Y2E8-2]
NP_056081.1. NM_015266.2. [Q9Y2E8-1]
UniGeneiHs.444202.

3D structure databases

ProteinModelPortaliQ9Y2E8.
SMRiQ9Y2E8. Positions 97-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116906. 1 interaction.
IntActiQ9Y2E8. 1 interaction.
STRINGi9606.ENSP00000354966.

Protein family/group databases

TCDBi2.A.36.1.9. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

PhosphoSiteiQ9Y2E8.

Polymorphism and mutation databases

BioMutaiSLC9A8.
DMDMi254763448.

Proteomic databases

MaxQBiQ9Y2E8.
PaxDbiQ9Y2E8.
PRIDEiQ9Y2E8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361573; ENSP00000354966; ENSG00000197818.
ENST00000417961; ENSP00000416418; ENSG00000197818. [Q9Y2E8-2]
GeneIDi23315.
KEGGihsa:23315.
UCSCiuc002xuv.2. human. [Q9Y2E8-1]
uc010zyj.2. human. [Q9Y2E8-2]

Organism-specific databases

CTDi23315.
GeneCardsiGC20P048429.
HGNCiHGNC:20728. SLC9A8.
HPAiCAB033682.
MIMi612730. gene.
neXtProtiNX_Q9Y2E8.
PharmGKBiPA134924114.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000172307.
HOVERGENiHBG056815.
InParanoidiQ9Y2E8.
KOiK14724.
OMAiDVIYKMT.
OrthoDBiEOG7H4DT6.
PhylomeDBiQ9Y2E8.
TreeFamiTF354313.

Enzyme and pathway databases

ReactomeiREACT_19314. Sodium/Proton exchangers.

Miscellaneous databases

ChiTaRSiSLC9A8. human.
GeneWikiiSLC9A8.
GenomeRNAii23315.
NextBioi45196.
PROiQ9Y2E8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y2E8.
CleanExiHS_SLC9A8.
GenevisibleiQ9Y2E8. HS.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-155 (ISOFORM 1).
    Tissue: Gastric mucosa and Placenta.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Small intestine.
  5. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  8. "Four Na+/H+ exchanger isoforms are distributed to Golgi and post-Golgi Compartments and are involved in organelle pH regulation."
    Nakamura N., Tanaka S., Teko Y., Mitsui K., Kanazawa H.
    J. Biol. Chem. 280:1561-1572(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-571 AND SER-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiSL9A8_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2E8
Secondary accession number(s): B4DTQ8
, Q2M1U9, Q68CZ8, Q9BX15, Q9Y507
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 126 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.