Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9Y2E8 (SL9A8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/hydrogen exchanger 8
Alternative name(s):
Na(+)/H(+) exchanger 8
Short name=NHE-8
Solute carrier family 9 member 8
Gene names
Name:SLC9A8
Synonyms:KIAA0939, NHE8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length581 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Ref.8

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein Ref.8.

Tissue specificity

Ubiquitous. Strongly expressed in skeletal muscle and kidney. Ref.8

Sequence similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Sequence caution

The sequence BAA76783.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y2E8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y2E8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     178-178: Q → QGFFFVCVCVFVCFILQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 581581Sodium/hydrogen exchanger 8
PRO_0000052365

Regions

Transmembrane60 – 8021Helical; Potential
Transmembrane84 – 10421Helical; Potential
Transmembrane123 – 14321Helical; Potential
Transmembrane156 – 17621Helical; Potential
Transmembrane191 – 21121Helical; Potential
Transmembrane264 – 28421Helical; Potential
Transmembrane311 – 33121Helical; Potential
Transmembrane354 – 37421Helical; Potential
Transmembrane379 – 39921Helical; Potential
Transmembrane417 – 43721Helical; Potential
Transmembrane451 – 47121Helical; Potential

Amino acid modifications

Modified residue5711Phosphoserine Ref.9
Modified residue5731Phosphoserine Ref.9

Natural variations

Alternative sequence1781Q → QGFFFVCVCVFVCFILQ in isoform 2.
VSP_037686

Experimental info

Sequence conflict1141N → D in BAC85475. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 28, 2009. Version 4.
Checksum: 7508B62B74D584B1

FASTA58165,422
        10         20         30         40         50         60 
MGEKMAEEER FPNTTHEGFN VTLHTTLVVT TKLVLPTPGK PILPVQTGEQ AQQEEQSSGM 

        70         80         90        100        110        120 
TIFFSLLVLA ICIILVHLLI RYRLHFLPES VAVVSLGILM GAVIKIIEFK KLANWKEEEM 

       130        140        150        160        170        180 
FRPNMFFLLL LPPIIFESGY SLHKGNFFQN IGSITLFAVF GTAISAFVVG GGIYFLGQAD 

       190        200        210        220        230        240 
VISKLNMTDS FAFGSLISAV DPVATIAIFN ALHVDPVLNM LVFGESILND AVSIVLTNTA 

       250        260        270        280        290        300 
EGLTRKNMSD VSGWQTFLQA LDYFLKMFFG SAALGTLTGL ISALVLKHID LRKTPSLEFG 

       310        320        330        340        350        360 
MMIIFAYLPY GLAEGISLSG IMAILFSGIV MSHYTHHNLS PVTQILMQQT LRTVAFLCET 

       370        380        390        400        410        420 
CVFAFLGLSI FSFPHKFEIS FVIWCIVLVL FGRAVNIFPL SYLLNFFRDH KITPKMMFIM 

       430        440        450        460        470        480 
WFSGLRGAIP YALSLHLDLE PMEKRQLIGT TTIVIVLFTI LLLGGSTMPL IRLMDIEDAK 

       490        500        510        520        530        540 
AHRRNKKDVN LSKTEKMGNT VESEHLSELT EEEYEAHYIR RQDLKGFVWL DAKYLNPFFT 

       550        560        570        580 
RRLTQEDLHH GRIQMKTLTN KWYEEVRQGP SGSEDDEQEL L 

« Hide

Isoform 2 [UniParc].

Checksum: F82E64383B1E4192
Show »

FASTA59767,275

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-155 (ISOFORM 1).
Tissue: Gastric mucosa and Placenta.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Small intestine.
[5]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]"Four Na+/H+ exchanger isoforms are distributed to Golgi and post-Golgi Compartments and are involved in organelle pH regulation."
Nakamura N., Tanaka S., Teko Y., Mitsui K., Kanazawa H.
J. Biol. Chem. 280:1561-1572(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-571 AND SER-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023156 mRNA. Translation: BAA76783.2. Different initiation.
AK131001 mRNA. Translation: BAC85475.1. Different termination.
AK300318 mRNA. Translation: BAG62070.1.
CR749638 mRNA. Translation: CAH18432.1.
AL162615, AL031685 Genomic DNA. Translation: CAI18970.1.
AL031685, AL162615 Genomic DNA. Translation: CAI23460.1.
CH471077 Genomic DNA. Translation: EAW75649.1.
BC112213 mRNA. Translation: AAI12214.1.
RefSeqNP_001247420.1. NM_001260491.1.
NP_056081.1. NM_015266.2.
UniGeneHs.444202.

3D structure databases

ProteinModelPortalQ9Y2E8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116906. 1 interaction.
IntActQ9Y2E8. 1 interaction.
STRING9606.ENSP00000354966.

Protein family/group databases

TCDB2.A.36.1.9. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

PhosphoSiteQ9Y2E8.

Polymorphism databases

DMDM254763448.

Proteomic databases

PaxDbQ9Y2E8.
PRIDEQ9Y2E8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361573; ENSP00000354966; ENSG00000197818. [Q9Y2E8-1]
ENST00000417961; ENSP00000416418; ENSG00000197818. [Q9Y2E8-2]
GeneID23315.
KEGGhsa:23315.
UCSCuc002xuv.2. human. [Q9Y2E8-1]
uc010zyj.2. human. [Q9Y2E8-2]

Organism-specific databases

CTD23315.
GeneCardsGC20P048429.
HGNCHGNC:20728. SLC9A8.
HPACAB033682.
MIM612730. gene.
neXtProtNX_Q9Y2E8.
PharmGKBPA134924114.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0025.
HOGENOMHOG000172307.
HOVERGENHBG056815.
InParanoidQ9Y2E8.
KOK14724.
OMAIKLSGIM.
OrthoDBEOG7H4DT6.
PhylomeDBQ9Y2E8.
TreeFamTF354313.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ9Y2E8.
BgeeQ9Y2E8.
CleanExHS_SLC9A8.
GenevestigatorQ9Y2E8.

Family and domain databases

InterProIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018409. Na/H_exchanger_8_eu.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF9. PTHR10110:SF9. 1 hit.
PfamPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSPR01084. NAHEXCHNGR.
TIGRFAMsTIGR00840. b_cpa1. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSLC9A8. human.
GeneWikiSLC9A8.
GenomeRNAi23315.
NextBio45196.
PROQ9Y2E8.
SOURCESearch...

Entry information

Entry nameSL9A8_HUMAN
AccessionPrimary (citable) accession number: Q9Y2E8
Secondary accession number(s): B4DTQ8 expand/collapse secondary AC list , Q2M1U9, Q68CZ8, Q9BX15, Q9Y507
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: July 28, 2009
Last modified: April 16, 2014
This is version 115 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM