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Q9Y2E8

- SL9A8_HUMAN

UniProt

Q9Y2E8 - SL9A8_HUMAN

Protein

Sodium/hydrogen exchanger 8

Gene

SLC9A8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 4 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.1 Publication

    GO - Molecular functioni

    1. potassium:proton antiporter activity Source: Ensembl
    2. sodium:proton antiporter activity Source: Ensembl

    GO - Biological processi

    1. ion transport Source: Reactome
    2. regulation of intracellular pH Source: Ensembl
    3. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Antiport, Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiREACT_19314. Sodium/Proton exchangers.

    Protein family/group databases

    TCDBi2.A.36.1.9. the monovalent cation:proton antiporter-1 (cpa1) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/hydrogen exchanger 8
    Alternative name(s):
    Na(+)/H(+) exchanger 8
    Short name:
    NHE-8
    Solute carrier family 9 member 8
    Gene namesi
    Name:SLC9A8
    Synonyms:KIAA0939, NHE8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:20728. SLC9A8.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. Golgi membrane Source: Reactome
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134924114.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 581581Sodium/hydrogen exchanger 8PRO_0000052365Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei571 – 5711Phosphoserine1 Publication
    Modified residuei573 – 5731Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9Y2E8.
    PRIDEiQ9Y2E8.

    PTM databases

    PhosphoSiteiQ9Y2E8.

    Expressioni

    Tissue specificityi

    Ubiquitous. Strongly expressed in skeletal muscle and kidney.1 Publication

    Gene expression databases

    ArrayExpressiQ9Y2E8.
    BgeeiQ9Y2E8.
    CleanExiHS_SLC9A8.
    GenevestigatoriQ9Y2E8.

    Organism-specific databases

    HPAiCAB033682.

    Interactioni

    Protein-protein interaction databases

    BioGridi116906. 1 interaction.
    IntActiQ9Y2E8. 1 interaction.
    STRINGi9606.ENSP00000354966.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y2E8.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei60 – 8021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei84 – 10421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei156 – 17621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei191 – 21121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei264 – 28421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei354 – 37421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei379 – 39921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei417 – 43721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei451 – 47121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0025.
    HOGENOMiHOG000172307.
    HOVERGENiHBG056815.
    InParanoidiQ9Y2E8.
    KOiK14724.
    OMAiERFPNTT.
    OrthoDBiEOG7H4DT6.
    PhylomeDBiQ9Y2E8.
    TreeFamiTF354313.

    Family and domain databases

    InterProiIPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR004709. NaH_exchanger.
    [Graphical view]
    PANTHERiPTHR10110. PTHR10110. 1 hit.
    PfamiPF00999. Na_H_Exchanger. 1 hit.
    [Graphical view]
    PRINTSiPR01084. NAHEXCHNGR.
    TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9Y2E8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGEKMAEEER FPNTTHEGFN VTLHTTLVVT TKLVLPTPGK PILPVQTGEQ    50
    AQQEEQSSGM TIFFSLLVLA ICIILVHLLI RYRLHFLPES VAVVSLGILM 100
    GAVIKIIEFK KLANWKEEEM FRPNMFFLLL LPPIIFESGY SLHKGNFFQN 150
    IGSITLFAVF GTAISAFVVG GGIYFLGQAD VISKLNMTDS FAFGSLISAV 200
    DPVATIAIFN ALHVDPVLNM LVFGESILND AVSIVLTNTA EGLTRKNMSD 250
    VSGWQTFLQA LDYFLKMFFG SAALGTLTGL ISALVLKHID LRKTPSLEFG 300
    MMIIFAYLPY GLAEGISLSG IMAILFSGIV MSHYTHHNLS PVTQILMQQT 350
    LRTVAFLCET CVFAFLGLSI FSFPHKFEIS FVIWCIVLVL FGRAVNIFPL 400
    SYLLNFFRDH KITPKMMFIM WFSGLRGAIP YALSLHLDLE PMEKRQLIGT 450
    TTIVIVLFTI LLLGGSTMPL IRLMDIEDAK AHRRNKKDVN LSKTEKMGNT 500
    VESEHLSELT EEEYEAHYIR RQDLKGFVWL DAKYLNPFFT RRLTQEDLHH 550
    GRIQMKTLTN KWYEEVRQGP SGSEDDEQEL L 581
    Length:581
    Mass (Da):65,422
    Last modified:July 28, 2009 - v4
    Checksum:i7508B62B74D584B1
    GO
    Isoform 2 (identifier: Q9Y2E8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         178-178: Q → QGFFFVCVCVFVCFILQ

    Show »
    Length:597
    Mass (Da):67,275
    Checksum:iF82E64383B1E4192
    GO

    Sequence cautioni

    The sequence BAA76783.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141N → D in BAC85475. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei178 – 1781Q → QGFFFVCVCVFVCFILQ in isoform 2. 1 PublicationVSP_037686

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023156 mRNA. Translation: BAA76783.2. Different initiation.
    AK131001 mRNA. Translation: BAC85475.1. Different termination.
    AK300318 mRNA. Translation: BAG62070.1.
    CR749638 mRNA. Translation: CAH18432.1.
    AL162615, AL031685 Genomic DNA. Translation: CAI18970.1.
    AL031685, AL162615 Genomic DNA. Translation: CAI23460.1.
    CH471077 Genomic DNA. Translation: EAW75649.1.
    BC112213 mRNA. Translation: AAI12214.1.
    CCDSiCCDS13421.1. [Q9Y2E8-1]
    CCDS58774.1. [Q9Y2E8-2]
    RefSeqiNP_001247420.1. NM_001260491.1. [Q9Y2E8-2]
    NP_056081.1. NM_015266.2. [Q9Y2E8-1]
    UniGeneiHs.444202.

    Genome annotation databases

    EnsembliENST00000361573; ENSP00000354966; ENSG00000197818. [Q9Y2E8-1]
    ENST00000417961; ENSP00000416418; ENSG00000197818. [Q9Y2E8-2]
    GeneIDi23315.
    KEGGihsa:23315.
    UCSCiuc002xuv.2. human. [Q9Y2E8-1]
    uc010zyj.2. human. [Q9Y2E8-2]

    Polymorphism databases

    DMDMi254763448.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023156 mRNA. Translation: BAA76783.2 . Different initiation.
    AK131001 mRNA. Translation: BAC85475.1 . Different termination.
    AK300318 mRNA. Translation: BAG62070.1 .
    CR749638 mRNA. Translation: CAH18432.1 .
    AL162615 , AL031685 Genomic DNA. Translation: CAI18970.1 .
    AL031685 , AL162615 Genomic DNA. Translation: CAI23460.1 .
    CH471077 Genomic DNA. Translation: EAW75649.1 .
    BC112213 mRNA. Translation: AAI12214.1 .
    CCDSi CCDS13421.1. [Q9Y2E8-1 ]
    CCDS58774.1. [Q9Y2E8-2 ]
    RefSeqi NP_001247420.1. NM_001260491.1. [Q9Y2E8-2 ]
    NP_056081.1. NM_015266.2. [Q9Y2E8-1 ]
    UniGenei Hs.444202.

    3D structure databases

    ProteinModelPortali Q9Y2E8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116906. 1 interaction.
    IntActi Q9Y2E8. 1 interaction.
    STRINGi 9606.ENSP00000354966.

    Protein family/group databases

    TCDBi 2.A.36.1.9. the monovalent cation:proton antiporter-1 (cpa1) family.

    PTM databases

    PhosphoSitei Q9Y2E8.

    Polymorphism databases

    DMDMi 254763448.

    Proteomic databases

    PaxDbi Q9Y2E8.
    PRIDEi Q9Y2E8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361573 ; ENSP00000354966 ; ENSG00000197818 . [Q9Y2E8-1 ]
    ENST00000417961 ; ENSP00000416418 ; ENSG00000197818 . [Q9Y2E8-2 ]
    GeneIDi 23315.
    KEGGi hsa:23315.
    UCSCi uc002xuv.2. human. [Q9Y2E8-1 ]
    uc010zyj.2. human. [Q9Y2E8-2 ]

    Organism-specific databases

    CTDi 23315.
    GeneCardsi GC20P048429.
    HGNCi HGNC:20728. SLC9A8.
    HPAi CAB033682.
    MIMi 612730. gene.
    neXtProti NX_Q9Y2E8.
    PharmGKBi PA134924114.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0025.
    HOGENOMi HOG000172307.
    HOVERGENi HBG056815.
    InParanoidi Q9Y2E8.
    KOi K14724.
    OMAi ERFPNTT.
    OrthoDBi EOG7H4DT6.
    PhylomeDBi Q9Y2E8.
    TreeFami TF354313.

    Enzyme and pathway databases

    Reactomei REACT_19314. Sodium/Proton exchangers.

    Miscellaneous databases

    ChiTaRSi SLC9A8. human.
    GeneWikii SLC9A8.
    GenomeRNAii 23315.
    NextBioi 45196.
    PROi Q9Y2E8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y2E8.
    Bgeei Q9Y2E8.
    CleanExi HS_SLC9A8.
    Genevestigatori Q9Y2E8.

    Family and domain databases

    InterProi IPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR004709. NaH_exchanger.
    [Graphical view ]
    PANTHERi PTHR10110. PTHR10110. 1 hit.
    Pfami PF00999. Na_H_Exchanger. 1 hit.
    [Graphical view ]
    PRINTSi PR01084. NAHEXCHNGR.
    TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
      Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
      DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-155 (ISOFORM 1).
      Tissue: Gastric mucosa and Placenta.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Small intestine.
    5. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    8. "Four Na+/H+ exchanger isoforms are distributed to Golgi and post-Golgi Compartments and are involved in organelle pH regulation."
      Nakamura N., Tanaka S., Teko Y., Mitsui K., Kanazawa H.
      J. Biol. Chem. 280:1561-1572(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-571 AND SER-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiSL9A8_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y2E8
    Secondary accession number(s): B4DTQ8
    , Q2M1U9, Q68CZ8, Q9BX15, Q9Y507
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 120 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3