Q9Y2E6 (DTX4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase DTX4
EC=6.3.2.-

Alternative name(s):
Protein deltex-4
Short name=Deltex4
RING finger protein 155

Gene names

Name:	DTX4
Synonyms:	KIAA0937, RNF155

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations By similarity. Functions as an ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4. Ref.4
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with NLRP4. Ref.4
Subcellular location	Cytoplasm By similarity.
Domain	The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases By similarity.
Sequence similarities	Belongs to the Deltex family. Contains 1 RING-type zinc finger. Contains 2 WWE domains.
Sequence caution	The sequence BAA76781.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Notch signaling pathway Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y2E6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y2E6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	E3 ubiquitin-protein ligase DTX4		PRO_0000280555
Regions
	Domain	1 – 78	78	WWE 1
	Domain	79 – 155	77	WWE 2
	Zinc finger	409 – 468	60	RING-type; atypical
Natural variations
	Alternative sequence	1 – 106	106	Missing in isoform 2.		VSP_023784

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: 5C851845B42630BD	FASTA	619	67,258
	10 20 30 40 50 60 MLLASAVVVW EWLNEHGRWR PYSPAVSHHI EAVVRAGPRA GGSVVLGQVD SRLAPYIIDL 70 80 90 100 110 120 QSMNQFRQDT GTLRPVRRNY YDPSSAPGKG VVWEWENDNG SWTPYDMEVG ITIQHAYEKQ 130 140 150 160 170 180 HPWIDLTSIG FSYVIDFNTM GQINRQTQRQ RRVRRRLDLI YPMVTGTLPK AQSWPVSPGP 190 200 210 220 230 240 ATSPPMSPCS CPQCVLVMSV KAAVVNGSTG PLQLPVTRKN MPPPGVVKLP PLPGSGAKPL 250 260 270 280 290 300 DSTGTIRGPL KTAPSQVIRR QASSMPTGTT MGSPASPPGP NSKTGRVALA TLNRTNLQRL 310 320 330 340 350 360 AIAQSRVLIA SGVPTVPVKN LNGSSPVNPA LAGITGILMS AAGLPVCLTR PPKLVLHPPP 370 380 390 400 410 420 VSKSEIKSIP GVSNTSRKTT KKQAKKGKTP EEVLKKYLQK VRHPPDEDCT ICMERLTAPS 430 440 450 460 470 480 GYKGPQPTVK PDLVGKLSRC GHVYHIYCLV AMYNNGNKDG SLQCPTCKTI YGVKTGTQPP 490 500 510 520 530 540 GKMEYHLIPH SLPGHPDCKT IRIIYSIPPG IQGPEHPNPG KSFSARGFPR HCYLPDSEKG 550 560 570 580 590 600 RKVLKLLLVA WDRRLIFAIG TSSTTGESDT VIWNEVHHKT EFGSNLTGHG YPDANYLDNV 610 LAELAAQGIS EDSTAQEKD
	Isoform 2 [UniParc]. Checksum: CBC72BC1D20CDD5C	FASTA	513	55,284
	10 20 30 40 50 60 MEVGITIQHA YEKQHPWIDL TSIGFSYVID FNTMGQINRQ TQRQRRVRRR LDLIYPMVTG 70 80 90 100 110 120 TLPKAQSWPV SPGPATSPPM SPCSCPQCVL VMSVKAAVVN GSTGPLQLPV TRKNMPPPGV 130 140 150 160 170 180 VKLPPLPGSG AKPLDSTGTI RGPLKTAPSQ VIRRQASSMP TGTTMGSPAS PPGPNSKTGR 190 200 210 220 230 240 VALATLNRTN LQRLAIAQSR VLIASGVPTV PVKNLNGSSP VNPALAGITG ILMSAAGLPV 250 260 270 280 290 300 CLTRPPKLVL HPPPVSKSEI KSIPGVSNTS RKTTKKQAKK GKTPEEVLKK YLQKVRHPPD 310 320 330 340 350 360 EDCTICMERL TAPSGYKGPQ PTVKPDLVGK LSRCGHVYHI YCLVAMYNNG NKDGSLQCPT 370 380 390 400 410 420 CKTIYGVKTG TQPPGKMEYH LIPHSLPGHP DCKTIRIIYS IPPGIQGPEH PNPGKSFSAR 430 440 450 460 470 480 GFPRHCYLPD SEKGRKVLKL LLVAWDRRLI FAIGTSSTTG ESDTVIWNEV HHKTEFGSNL 490 500 510 TGHGYPDANY LDNVLAELAA QGISEDSTAQ EKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"Normal immune system development in mice lacking the Deltex-1 RING finger domain." Storck S., Delbos F., Stadler N., Thirion-Delalande C., Bernex F., Verthuy C., Ferrier P., Weill J.-C., Reynaud C.-A. Mol. Cell. Biol. 25:1437-1445(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[4]	"NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4." Cui J., Li Y., Zhu L., Liu D., Songyang Z., Wang H.Y., Wang R.F. Nat. Immunol. 13:387-395(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS E3 LIGASE, INTERACTION WITH NLRP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023154 mRNA. Translation: BAA76781.1. Different initiation. BC119011 mRNA. Translation: AAI19012.1. BC122861 mRNA. Translation: AAI22862.1.
IPI	IPI00847948. IPI00848268.
RefSeq	NP_055992.1. NM_015177.1.
UniGene	Hs.523696.
3D structure databases
ProteinModelPortal	Q9Y2E6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y2E6. 1 interaction.
STRING	9606.ENSP00000227451.
PTM databases
PhosphoSite	Q9Y2E6.
Polymorphism databases
DMDM	134034097.
Proteomic databases
PaxDb	Q9Y2E6.
PRIDE	Q9Y2E6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000227451; ENSP00000227451; ENSG00000110042. ENST00000532982; ENSP00000434055; ENSG00000110042.
GeneID	23220.
KEGG	hsa:23220.
UCSC	uc001nnr.2. human.
Organism-specific databases
CTD	23220.
GeneCards	GC11P058939.
HGNC	HGNC:29151. DTX4.
neXtProt	NX_Q9Y2E6.
PharmGKB	PA134930720.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG84763.
HOGENOM	HOG000007352.
HOVERGEN	HBG007213.
InParanoid	Q9Y2E6.
KO	K06058.
OMA	MNQFRQD.
OrthoDB	EOG4QC152.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
SignaLink	Q9Y2E6.
UniPathway	UPA00143.
Gene expression databases
Bgee	Q9Y2E6.
CleanEx	HS_DTX4.
Genevestigator	Q9Y2E6.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR004170. WWE-dom. IPR018123. WWE-dom_subgr. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF02825. WWE. 2 hits. PF13639. zf-RING_2. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00678. WWE. 2 hits. [Graphical view]
PROSITE	PS50918. WWE. 2 hits. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DTX4. human.
GenomeRNAi	23220.
NextBio	44807.

Entry information

Entry name

DTX4_HUMAN

Accession

Primary (citable) accession number: Q9Y2E6
Secondary accession number(s): Q0VF38

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	May 29, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.