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Protein

Epididymis-specific alpha-mannosidase

Gene

MAN2B2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36ZincBy similarity1
Metal bindingi38ZincBy similarity1
Active sitei151NucleophileBy similarity1
Metal bindingi151ZincBy similarity1
Metal bindingi420ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS00341-MONOMER.
ReactomeiR-HSA-8853383. Lysosomal oligosaccharide catabolism.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
Alternative name(s):
Mannosidase alpha class 2B member 2
Gene namesi
Name:MAN2B2
Synonyms:KIAA0935
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:29623. MAN2B2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • lysosomal lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi23324.
OpenTargetsiENSG00000013288.
PharmGKBiPA128394625.

Chemistry databases

ChEMBLiCHEMBL2682.

Polymorphism and mutation databases

BioMutaiMAN2B2.
DMDMi296439484.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001207724 – 1009Epididymis-specific alpha-mannosidaseAdd BLAST986

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi226N-linked (GlcNAc...)Sequence analysis1
Glycosylationi249N-linked (GlcNAc...)Sequence analysis1
Glycosylationi294N-linked (GlcNAc...)Sequence analysis1
Glycosylationi336N-linked (GlcNAc...)Sequence analysis1
Glycosylationi516N-linked (GlcNAc...)1 Publication1
Glycosylationi608N-linked (GlcNAc...)Sequence analysis1
Glycosylationi670N-linked (GlcNAc...)Sequence analysis1
Glycosylationi675N-linked (GlcNAc...)Sequence analysis1
Glycosylationi748N-linked (GlcNAc...)Sequence analysis1
Glycosylationi808N-linked (GlcNAc...)Sequence analysis1
Glycosylationi812N-linked (GlcNAc...)Sequence analysis1
Glycosylationi890N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9Y2E5.
PaxDbiQ9Y2E5.
PeptideAtlasiQ9Y2E5.
PRIDEiQ9Y2E5.

PTM databases

iPTMnetiQ9Y2E5.
PhosphoSitePlusiQ9Y2E5.

Expressioni

Gene expression databases

BgeeiENSG00000013288.
CleanExiHS_MAN2B2.
ExpressionAtlasiQ9Y2E5. baseline and differential.
GenevisibleiQ9Y2E5. HS.

Organism-specific databases

HPAiHPA037005.

Interactioni

Protein-protein interaction databases

BioGridi116912. 6 interactors.
IntActiQ9Y2E5. 1 interactor.
STRINGi9606.ENSP00000285599.

Chemistry databases

BindingDBiQ9Y2E5.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2E5.
SMRiQ9Y2E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000113450.
HOVERGENiHBG052392.
InParanoidiQ9Y2E5.
KOiK12312.
OMAiYSTEPFQ.
OrthoDBiEOG091G019Q.
PhylomeDBiQ9Y2E5.
TreeFamiTF332447.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y2E5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQLCWLPLL APLLLLRPPG VQSAGPIRAF VVPHSHMDVG WVYTVQESMR
60 70 80 90 100
AYAANVYTSV VEELARGQQR RFIAVEQEFF RLWWDGVASD QQKYQVRQLL
110 120 130 140 150
EEGRLEFVIG GQVMHDEAVT HLDDQILQLT EGHGFLYETF GIRPQFSWHV
160 170 180 190 200
DPFGASATTP TLFALAGFNA HLGSRIDYDL KAAMQEARGL QFVWRGSPSL
210 220 230 240 250
SERQEIFTHI MDQYSYCTPS HIPFSNRSGF YWNGVAVFPK PPQDGVYPNM
260 270 280 290 300
SEPVTPANIN LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFA
310 320 330 340 350
NMDPLLDHIN SHAAELGVSV QYATLGDYFR ALHALNVTWR VRDHHDFLPY
360 370 380 390 400
STEPFQAWTG FYTSRSSLKG LARRASALLY AGESMFTRYL WPAPRGHLDP
410 420 430 440 450
TWALQQLQQL RWAVSEVQHH DAITGTESPK VRDMYATHLA SGMLGMRKLM
460 470 480 490 500
ASIVLDELQP QAPMAASSDA GPAGHFASVY NPLAWTVTTI VTLTVGFPGV
510 520 530 540 550
RVTDEAGHPV PSQIQNSTET PSAYDLLILT TIPGLSYRHY NIRPTAGAQE
560 570 580 590 600
GTQEPAATVA STLQFGRRLR RRTSHAGRYL VPVANDCYIV LLDQDTNLMH
610 620 630 640 650
SIWERQSNRT VRVTQEFLEY HVNGDVKQGP ISDNYLFTPG KAAVPAWEAV
660 670 680 690 700
EMEIVAGQLV TEIRQYFYRN MTAQNYTYAI RSRLTHVPQG HDGELLCHRI
710 720 730 740 750
EQEYQAGPLE LNREAVLRTS TNLNSQQVIY SDNNGYQMQR RPYVSYVNNS
760 770 780 790 800
IARNYYPMVQ SAFMEDGKSR LVLLSERAHG ISSQGNGQVE VMLHRRLWNN
810 820 830 840 850
FDWDLGYNLT LNDTSVVHPV LWLLLGSWSL TTALRQRSAL ALQHRPVVLF
860 870 880 890 900
GDLAGTAPKL PGPQQQEAVT LPPNLHLQIL SIPGWRYSSN HTEHSQNLRK
910 920 930 940 950
GHRGEAQADL RRVLLRLYHL YEVGEDPVLS QPVTVNLEAV LQALGSVVAV
960 970 980 990 1000
EERSLTGTWD LSMLHRWSWR TGPGRHRGDT TSPSRPPGGP IITVHPKEIR

TFFIHFQQQ
Length:1,009
Mass (Da):113,979
Last modified:May 18, 2010 - v4
Checksum:i21BB879400E0F387
GO
Isoform 2 (identifier: Q9Y2E5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     939-1009: AVLQALGSVV...RTFFIHFQQQ → VNFPTPIQTISQGSKP

Note: No experimental confirmation available.
Show »
Length:954
Mass (Da):107,704
Checksum:i9EF3191B5AA34B4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79F → S in CAD89971 (PubMed:17974005).Curated1
Sequence conflicti938E → Q in CAD89971 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025328243Q → P.2 PublicationsCorresponds to variant rs2301796dbSNPEnsembl.1
Natural variantiVAR_025329320V → M.1 PublicationCorresponds to variant rs2301795dbSNPEnsembl.1
Natural variantiVAR_055840365R → C.Corresponds to variant rs6858328dbSNPEnsembl.1
Natural variantiVAR_025330446M → V.2 PublicationsCorresponds to variant rs2301790dbSNPEnsembl.1
Natural variantiVAR_025331541N → S.2 PublicationsCorresponds to variant rs2301788dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013816939 – 1009AVLQA…HFQQQ → VNFPTPIQTISQGSKP in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL833071 mRNA. Translation: CAD89971.1.
AC004480 Genomic DNA. No translation available.
BC033307 mRNA. Translation: AAH33307.1.
AB023152 mRNA. Translation: BAA76779.2.
CCDSiCCDS33951.1. [Q9Y2E5-1]
RefSeqiNP_001278967.1. NM_001292038.1.
NP_056089.1. NM_015274.2. [Q9Y2E5-1]
UniGeneiHs.188464.

Genome annotation databases

EnsembliENST00000285599; ENSP00000285599; ENSG00000013288. [Q9Y2E5-1]
GeneIDi23324.
KEGGihsa:23324.
UCSCiuc003gjf.2. human. [Q9Y2E5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL833071 mRNA. Translation: CAD89971.1.
AC004480 Genomic DNA. No translation available.
BC033307 mRNA. Translation: AAH33307.1.
AB023152 mRNA. Translation: BAA76779.2.
CCDSiCCDS33951.1. [Q9Y2E5-1]
RefSeqiNP_001278967.1. NM_001292038.1.
NP_056089.1. NM_015274.2. [Q9Y2E5-1]
UniGeneiHs.188464.

3D structure databases

ProteinModelPortaliQ9Y2E5.
SMRiQ9Y2E5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116912. 6 interactors.
IntActiQ9Y2E5. 1 interactor.
STRINGi9606.ENSP00000285599.

Chemistry databases

BindingDBiQ9Y2E5.
ChEMBLiCHEMBL2682.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

PTM databases

iPTMnetiQ9Y2E5.
PhosphoSitePlusiQ9Y2E5.

Polymorphism and mutation databases

BioMutaiMAN2B2.
DMDMi296439484.

Proteomic databases

EPDiQ9Y2E5.
PaxDbiQ9Y2E5.
PeptideAtlasiQ9Y2E5.
PRIDEiQ9Y2E5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000285599; ENSP00000285599; ENSG00000013288. [Q9Y2E5-1]
GeneIDi23324.
KEGGihsa:23324.
UCSCiuc003gjf.2. human. [Q9Y2E5-1]

Organism-specific databases

CTDi23324.
DisGeNETi23324.
GeneCardsiMAN2B2.
H-InvDBHIX0200620.
HGNCiHGNC:29623. MAN2B2.
HPAiHPA037005.
neXtProtiNX_Q9Y2E5.
OpenTargetsiENSG00000013288.
PharmGKBiPA128394625.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000113450.
HOVERGENiHBG052392.
InParanoidiQ9Y2E5.
KOiK12312.
OMAiYSTEPFQ.
OrthoDBiEOG091G019Q.
PhylomeDBiQ9Y2E5.
TreeFamiTF332447.

Enzyme and pathway databases

BioCyciZFISH:HS00341-MONOMER.
ReactomeiR-HSA-8853383. Lysosomal oligosaccharide catabolism.

Miscellaneous databases

ChiTaRSiMAN2B2. human.
GeneWikiiMAN2B2.
GenomeRNAii23324.
PROiQ9Y2E5.

Gene expression databases

BgeeiENSG00000013288.
CleanExiHS_MAN2B2.
ExpressionAtlasiQ9Y2E5. baseline and differential.
GenevisibleiQ9Y2E5. HS.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMA2B2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2E5
Secondary accession number(s): Q66MP2, Q86T67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.