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Q9Y2E5 (MA2B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epididymis-specific alpha-mannosidase

EC=3.2.1.24
Alternative name(s):
Mannosidase alpha class 2B member 2
Gene names
Name:MAN2B2
Synonyms:KIAA0935
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1009 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmannose metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosome

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionalpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y2E5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y2E5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     939-1009: AVLQALGSVV...RTFFIHFQQQ → VNFPTPIQTISQGSKP
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 1009986Epididymis-specific alpha-mannosidase
PRO_0000012077

Sites

Active site1511Nucleophile By similarity
Metal binding361Zinc By similarity
Metal binding381Zinc By similarity
Metal binding1511Zinc By similarity
Metal binding4201Zinc By similarity

Amino acid modifications

Glycosylation2261N-linked (GlcNAc...) Potential
Glycosylation2491N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential
Glycosylation5161N-linked (GlcNAc...) Ref.6
Glycosylation6081N-linked (GlcNAc...) Potential
Glycosylation6701N-linked (GlcNAc...) Potential
Glycosylation6751N-linked (GlcNAc...) Potential
Glycosylation7481N-linked (GlcNAc...) Potential
Glycosylation8081N-linked (GlcNAc...) Potential
Glycosylation8121N-linked (GlcNAc...) Potential
Glycosylation8901N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence939 – 100971AVLQA…HFQQQ → VNFPTPIQTISQGSKP in isoform 2.
VSP_013816
Natural variant2431Q → P. Ref.3 Ref.4
Corresponds to variant rs2301796 [ dbSNP | Ensembl ].
VAR_025328
Natural variant3201V → M. Ref.1
Corresponds to variant rs2301795 [ dbSNP | Ensembl ].
VAR_025329
Natural variant3651R → C.
Corresponds to variant rs6858328 [ dbSNP | Ensembl ].
VAR_055840
Natural variant4461M → V. Ref.3 Ref.4
Corresponds to variant rs2301790 [ dbSNP | Ensembl ].
VAR_025330
Natural variant5411N → S. Ref.3 Ref.4
Corresponds to variant rs2301788 [ dbSNP | Ensembl ].
VAR_025331

Experimental info

Sequence conflict791F → S in CAD89971. Ref.1
Sequence conflict9381E → Q in CAD89971. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 4.
Checksum: 21BB879400E0F387

FASTA1,009113,979
        10         20         30         40         50         60 
MGQLCWLPLL APLLLLRPPG VQSAGPIRAF VVPHSHMDVG WVYTVQESMR AYAANVYTSV 

        70         80         90        100        110        120 
VEELARGQQR RFIAVEQEFF RLWWDGVASD QQKYQVRQLL EEGRLEFVIG GQVMHDEAVT 

       130        140        150        160        170        180 
HLDDQILQLT EGHGFLYETF GIRPQFSWHV DPFGASATTP TLFALAGFNA HLGSRIDYDL 

       190        200        210        220        230        240 
KAAMQEARGL QFVWRGSPSL SERQEIFTHI MDQYSYCTPS HIPFSNRSGF YWNGVAVFPK 

       250        260        270        280        290        300 
PPQDGVYPNM SEPVTPANIN LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFA 

       310        320        330        340        350        360 
NMDPLLDHIN SHAAELGVSV QYATLGDYFR ALHALNVTWR VRDHHDFLPY STEPFQAWTG 

       370        380        390        400        410        420 
FYTSRSSLKG LARRASALLY AGESMFTRYL WPAPRGHLDP TWALQQLQQL RWAVSEVQHH 

       430        440        450        460        470        480 
DAITGTESPK VRDMYATHLA SGMLGMRKLM ASIVLDELQP QAPMAASSDA GPAGHFASVY 

       490        500        510        520        530        540 
NPLAWTVTTI VTLTVGFPGV RVTDEAGHPV PSQIQNSTET PSAYDLLILT TIPGLSYRHY 

       550        560        570        580        590        600 
NIRPTAGAQE GTQEPAATVA STLQFGRRLR RRTSHAGRYL VPVANDCYIV LLDQDTNLMH 

       610        620        630        640        650        660 
SIWERQSNRT VRVTQEFLEY HVNGDVKQGP ISDNYLFTPG KAAVPAWEAV EMEIVAGQLV 

       670        680        690        700        710        720 
TEIRQYFYRN MTAQNYTYAI RSRLTHVPQG HDGELLCHRI EQEYQAGPLE LNREAVLRTS 

       730        740        750        760        770        780 
TNLNSQQVIY SDNNGYQMQR RPYVSYVNNS IARNYYPMVQ SAFMEDGKSR LVLLSERAHG 

       790        800        810        820        830        840 
ISSQGNGQVE VMLHRRLWNN FDWDLGYNLT LNDTSVVHPV LWLLLGSWSL TTALRQRSAL 

       850        860        870        880        890        900 
ALQHRPVVLF GDLAGTAPKL PGPQQQEAVT LPPNLHLQIL SIPGWRYSSN HTEHSQNLRK 

       910        920        930        940        950        960 
GHRGEAQADL RRVLLRLYHL YEVGEDPVLS QPVTVNLEAV LQALGSVVAV EERSLTGTWD 

       970        980        990       1000 
LSMLHRWSWR TGPGRHRGDT TSPSRPPGGP IITVHPKEIR TFFIHFQQQ 

« Hide

Isoform 2 [UniParc].

Checksum: 9EF3191B5AA34B4F
Show »

FASTA954107,704

References

« Hide 'large scale' references
[1]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-320.
Tissue: Spinal cord.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS PRO-243; VAL-446 AND SER-541.
Tissue: Brain.
[4]"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1009 (ISOFORM 2), VARIANTS PRO-243; VAL-446 AND SER-541.
Tissue: Brain.
[5]Ohara O., Nagase T., Kikuno R.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[6]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-516.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL833071 mRNA. Translation: CAD89971.1.
AC004480 Genomic DNA. No translation available.
BC033307 mRNA. Translation: AAH33307.1.
AB023152 mRNA. Translation: BAA76779.2.
CCDSCCDS33951.1. [Q9Y2E5-1]
RefSeqNP_056089.1. NM_015274.2. [Q9Y2E5-1]
UniGeneHs.188464.

3D structure databases

ProteinModelPortalQ9Y2E5.
SMRQ9Y2E5. Positions 27-818.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116912. 2 interactions.

Chemistry

ChEMBLCHEMBL2682.

Protein family/group databases

CAZyGH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSiteQ9Y2E5.

Polymorphism databases

DMDM296439484.

Proteomic databases

MaxQBQ9Y2E5.
PaxDbQ9Y2E5.
PRIDEQ9Y2E5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000285599; ENSP00000285599; ENSG00000013288. [Q9Y2E5-1]
GeneID23324.
KEGGhsa:23324.
UCSCuc003gje.1. human. [Q9Y2E5-2]
uc003gjf.1. human. [Q9Y2E5-1]

Organism-specific databases

CTD23324.
GeneCardsGC04P006509.
H-InvDBHIX0200620.
HGNCHGNC:29623. MAN2B2.
HPAHPA037005.
neXtProtNX_Q9Y2E5.
PharmGKBPA128394625.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG306356.
HOGENOMHOG000113450.
HOVERGENHBG052392.
InParanoidQ9Y2E5.
KOK12312.
OMAMRAYAAN.
OrthoDBEOG7RFTGG.
PhylomeDBQ9Y2E5.
TreeFamTF332447.

Gene expression databases

ArrayExpressQ9Y2E5.
BgeeQ9Y2E5.
CleanExHS_MAN2B2.
GenevestigatorQ9Y2E5.

Family and domain databases

Gene3D1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Other

ChiTaRSMAN2B2. human.
GeneWikiMAN2B2.
GenomeRNAi23324.
NextBio45224.
PROQ9Y2E5.

Entry information

Entry nameMA2B2_HUMAN
AccessionPrimary (citable) accession number: Q9Y2E5
Secondary accession number(s): Q66MP2, Q86T67
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries