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Q9Y2E5

- MA2B2_HUMAN

UniProt

Q9Y2E5 - MA2B2_HUMAN

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Protein

Epididymis-specific alpha-mannosidase

Gene

MAN2B2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361ZincBy similarity
Metal bindingi38 – 381ZincBy similarity
Active sitei151 – 1511NucleophileBy similarity
Metal bindingi151 – 1511ZincBy similarity
Metal bindingi420 – 4201ZincBy similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
Alternative name(s):
Mannosidase alpha class 2B member 2
Gene namesi
Name:MAN2B2
Synonyms:KIAA0935
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:29623. MAN2B2.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProtKB
  2. lysosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394625.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 1009986Epididymis-specific alpha-mannosidasePRO_0000012077Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi294 – 2941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi516 – 5161N-linked (GlcNAc...)1 Publication
Glycosylationi608 – 6081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi670 – 6701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi675 – 6751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi748 – 7481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi808 – 8081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi812 – 8121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi890 – 8901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9Y2E5.
PaxDbiQ9Y2E5.
PRIDEiQ9Y2E5.

PTM databases

PhosphoSiteiQ9Y2E5.

Expressioni

Gene expression databases

BgeeiQ9Y2E5.
CleanExiHS_MAN2B2.
ExpressionAtlasiQ9Y2E5. baseline and differential.
GenevestigatoriQ9Y2E5.

Organism-specific databases

HPAiHPA037005.

Interactioni

Protein-protein interaction databases

BioGridi116912. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9Y2E5.
SMRiQ9Y2E5. Positions 27-818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG306356.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000113450.
HOVERGENiHBG052392.
InParanoidiQ9Y2E5.
KOiK12312.
OMAiMRAYAAN.
OrthoDBiEOG7RFTGG.
PhylomeDBiQ9Y2E5.
TreeFamiTF332447.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Y2E5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQLCWLPLL APLLLLRPPG VQSAGPIRAF VVPHSHMDVG WVYTVQESMR
60 70 80 90 100
AYAANVYTSV VEELARGQQR RFIAVEQEFF RLWWDGVASD QQKYQVRQLL
110 120 130 140 150
EEGRLEFVIG GQVMHDEAVT HLDDQILQLT EGHGFLYETF GIRPQFSWHV
160 170 180 190 200
DPFGASATTP TLFALAGFNA HLGSRIDYDL KAAMQEARGL QFVWRGSPSL
210 220 230 240 250
SERQEIFTHI MDQYSYCTPS HIPFSNRSGF YWNGVAVFPK PPQDGVYPNM
260 270 280 290 300
SEPVTPANIN LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFA
310 320 330 340 350
NMDPLLDHIN SHAAELGVSV QYATLGDYFR ALHALNVTWR VRDHHDFLPY
360 370 380 390 400
STEPFQAWTG FYTSRSSLKG LARRASALLY AGESMFTRYL WPAPRGHLDP
410 420 430 440 450
TWALQQLQQL RWAVSEVQHH DAITGTESPK VRDMYATHLA SGMLGMRKLM
460 470 480 490 500
ASIVLDELQP QAPMAASSDA GPAGHFASVY NPLAWTVTTI VTLTVGFPGV
510 520 530 540 550
RVTDEAGHPV PSQIQNSTET PSAYDLLILT TIPGLSYRHY NIRPTAGAQE
560 570 580 590 600
GTQEPAATVA STLQFGRRLR RRTSHAGRYL VPVANDCYIV LLDQDTNLMH
610 620 630 640 650
SIWERQSNRT VRVTQEFLEY HVNGDVKQGP ISDNYLFTPG KAAVPAWEAV
660 670 680 690 700
EMEIVAGQLV TEIRQYFYRN MTAQNYTYAI RSRLTHVPQG HDGELLCHRI
710 720 730 740 750
EQEYQAGPLE LNREAVLRTS TNLNSQQVIY SDNNGYQMQR RPYVSYVNNS
760 770 780 790 800
IARNYYPMVQ SAFMEDGKSR LVLLSERAHG ISSQGNGQVE VMLHRRLWNN
810 820 830 840 850
FDWDLGYNLT LNDTSVVHPV LWLLLGSWSL TTALRQRSAL ALQHRPVVLF
860 870 880 890 900
GDLAGTAPKL PGPQQQEAVT LPPNLHLQIL SIPGWRYSSN HTEHSQNLRK
910 920 930 940 950
GHRGEAQADL RRVLLRLYHL YEVGEDPVLS QPVTVNLEAV LQALGSVVAV
960 970 980 990 1000
EERSLTGTWD LSMLHRWSWR TGPGRHRGDT TSPSRPPGGP IITVHPKEIR

TFFIHFQQQ
Length:1,009
Mass (Da):113,979
Last modified:May 18, 2010 - v4
Checksum:i21BB879400E0F387
GO
Isoform 2 (identifier: Q9Y2E5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     939-1009: AVLQALGSVV...RTFFIHFQQQ → VNFPTPIQTISQGSKP

Note: No experimental confirmation available.

Show »
Length:954
Mass (Da):107,704
Checksum:i9EF3191B5AA34B4F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791F → S in CAD89971. (PubMed:17974005)Curated
Sequence conflicti938 – 9381E → Q in CAD89971. (PubMed:17974005)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti243 – 2431Q → P.2 Publications
Corresponds to variant rs2301796 [ dbSNP | Ensembl ].
VAR_025328
Natural varianti320 – 3201V → M.1 Publication
Corresponds to variant rs2301795 [ dbSNP | Ensembl ].
VAR_025329
Natural varianti365 – 3651R → C.
Corresponds to variant rs6858328 [ dbSNP | Ensembl ].
VAR_055840
Natural varianti446 – 4461M → V.2 Publications
Corresponds to variant rs2301790 [ dbSNP | Ensembl ].
VAR_025330
Natural varianti541 – 5411N → S.2 Publications
Corresponds to variant rs2301788 [ dbSNP | Ensembl ].
VAR_025331

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei939 – 100971AVLQA…HFQQQ → VNFPTPIQTISQGSKP in isoform 2. 1 PublicationVSP_013816Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL833071 mRNA. Translation: CAD89971.1.
AC004480 Genomic DNA. No translation available.
BC033307 mRNA. Translation: AAH33307.1.
AB023152 mRNA. Translation: BAA76779.2.
CCDSiCCDS33951.1. [Q9Y2E5-1]
RefSeqiNP_001278967.1. NM_001292038.1.
NP_056089.1. NM_015274.2. [Q9Y2E5-1]
UniGeneiHs.188464.

Genome annotation databases

EnsembliENST00000285599; ENSP00000285599; ENSG00000013288. [Q9Y2E5-1]
GeneIDi23324.
KEGGihsa:23324.
UCSCiuc003gje.1. human. [Q9Y2E5-2]
uc003gjf.1. human. [Q9Y2E5-1]

Polymorphism databases

DMDMi296439484.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL833071 mRNA. Translation: CAD89971.1 .
AC004480 Genomic DNA. No translation available.
BC033307 mRNA. Translation: AAH33307.1 .
AB023152 mRNA. Translation: BAA76779.2 .
CCDSi CCDS33951.1. [Q9Y2E5-1 ]
RefSeqi NP_001278967.1. NM_001292038.1.
NP_056089.1. NM_015274.2. [Q9Y2E5-1 ]
UniGenei Hs.188464.

3D structure databases

ProteinModelPortali Q9Y2E5.
SMRi Q9Y2E5. Positions 27-818.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116912. 4 interactions.

Chemistry

ChEMBLi CHEMBL2682.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSitei Q9Y2E5.

Polymorphism databases

DMDMi 296439484.

Proteomic databases

MaxQBi Q9Y2E5.
PaxDbi Q9Y2E5.
PRIDEi Q9Y2E5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000285599 ; ENSP00000285599 ; ENSG00000013288 . [Q9Y2E5-1 ]
GeneIDi 23324.
KEGGi hsa:23324.
UCSCi uc003gje.1. human. [Q9Y2E5-2 ]
uc003gjf.1. human. [Q9Y2E5-1 ]

Organism-specific databases

CTDi 23324.
GeneCardsi GC04P006509.
H-InvDB HIX0200620.
HGNCi HGNC:29623. MAN2B2.
HPAi HPA037005.
neXtProti NX_Q9Y2E5.
PharmGKBi PA128394625.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG306356.
GeneTreei ENSGT00510000046304.
HOGENOMi HOG000113450.
HOVERGENi HBG052392.
InParanoidi Q9Y2E5.
KOi K12312.
OMAi MRAYAAN.
OrthoDBi EOG7RFTGG.
PhylomeDBi Q9Y2E5.
TreeFami TF332447.

Miscellaneous databases

ChiTaRSi MAN2B2. human.
GeneWikii MAN2B2.
GenomeRNAii 23324.
NextBioi 45224.
PROi Q9Y2E5.

Gene expression databases

Bgeei Q9Y2E5.
CleanExi HS_MAN2B2.
ExpressionAtlasi Q9Y2E5. baseline and differential.
Genevestigatori Q9Y2E5.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-320.
    Tissue: Spinal cord.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS PRO-243; VAL-446 AND SER-541.
    Tissue: Brain.
  4. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1009 (ISOFORM 2), VARIANTS PRO-243; VAL-446 AND SER-541.
    Tissue: Brain.
  5. Ohara O., Nagase T., Kikuno R.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  6. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-516.
    Tissue: Liver.

Entry informationi

Entry nameiMA2B2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y2E5
Secondary accession number(s): Q66MP2, Q86T67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 18, 2010
Last modified: October 29, 2014
This is version 121 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3